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Toxoplasmosis is a zoonotic disease caused by the intracellular parasite Toxoplasma gondii. Although the definitive hosts are cats, other species can act as reservoirs, including livestock. In pigs, the infection is subclinical, so its relevance as a silent reservoir could be greater. The data related to the seroprevalence of T. gondii in pigs differ according to different factors, including methodology used, country, farm type, and location, and epidemiological factors, such as sex or age. The seroprevalence of T. gondii in pigs housed in intensive systems, specifically medium-sized farms with animal entry control systems, was evaluated, as well as epidemiological data that could be risk factors, such as age or sex. For this purpose, serum samples were obtained from a total of 92 pigs with ages ranging from 0 to 24 months, housed in sixteen intensive system farms located in eastern Spain. The seroprevalence was estimated as the number of positive animals by the ELISA method. The results obtained were a low seroprevalence (3.26%), and only age was associated with higher seroprevalence, being higher in animals older than 12 months. Further studies are necessary to elucidate the factors associated with seroprevalence and the security measures to eliminate the presence of this parasite in porcine farms.

Celiac disease (CD) is an autoimmune enteropathy triggered by dietary gluten, affecting 1% of the global population. The only currently available treatment is the gluten-free (GF) diet, which is often associated with nutritional imbalances and deficiencies in both macronutrients and micronutrients, particularly folate. This study aimed first to assess the folate intake of children and adolescents with CD (n = 68) compared to healthy controls (n = 65) using three 24-hour dietary recalls and a food frequency questionnaire. Secondly, to evaluate the vitamin composition of cereal-based GF products available in the Spanish market, using a previously developed GF food database (GLUTENFREE-2019). And finally, simulate two scenarios of GF product fortification with folic acid (low (60 μg/100 g) and high (280 μg/100 g) as a strategy to prevent folate deficiency in children and adolescents with CD. Dietary assessments showed that folate intake among CD patients was significantly lower than that of controls (p = 0.006) and fell below 80% of the Dietary Reference Values (DRV), suggesting a higher risk of folate deficiency within youngsters with CD. The analysis of 629 GF products revealed that only ten were fortified with folic acid. The fortification model at a concentration of 60 μg/100 g proved to be adequate to cover DRV of children and adolescents with CD without exceeding tolerable upper intake levels. In conclusion, possible folate deficiencies in the young population with CD could be reduced by fortification of commercially available GF products.










