Papers by Dipeshkumar Patel
International Journal of Current Microbiology and Applied Sciences, Oct 10, 2021
International Journal of Current Microbiology and Applied Sciences, Sep 10, 2021
European Chemical Bulletin , 2023
Biofertilizers are microorganisms that come from the soil's rhizosphere or root nodules. They enr... more Biofertilizers are microorganisms that come from the soil's rhizosphere or root nodules. They enrich the soil by increasing the crop's access to nutrients and they decrease the use of chemical fertilizers, which protects the environment. The two main plant nutrients known as the master essential elements in crop production are nitrogen and phosphorus. Beneficial microbes that can fix atmospheric nitrogen or solubilize phosphorus in the soil are some of the beneficial microbes utilized in biofertilizers. Other beneficial microbes include N2 fixing bacteria. In the current investigation, the rhizosphere of chilies in Anand District yielded fifteen Azotobacter isolates and five phosphate solubilizing fungal (PSF) isolates. All isolates were recognized using morphological, cultural, microscopic, and several biochemical assays. The results of yield parameters revealed that the treatment, 100% RDF + commercial Azotobacter + commercial PSF, showed the highest number of fruits (41.67/ plant), number of flowers (55.67 / plant), length of fruit (9.60 cm) and green Chili yield (25.23 t/ ha) was on par with treatment, 100% RDF + Efficient Azotobacter + Efficient PSF i.e., number of flowers (52.87 / plant), number of fruits (39.00 / plant), length of fruit (8.80 cm) and green chili yield (21.69 t/ ha). Whereas, highest dry matter weight (102.21 g), available N (178.75 Kg/ha), P (22.03 Kg/ha) and K (187.22 Kg/ha) was showed by treatment, 100% RDF + commercial Azotobacter + commercial PSF which was on par with treatment, 100% RDF + Efficient Azotobacter + Efficient PSF i.e., dry matter weight (98.00 g), available N (176.66 Kg/ha), P (21.59 Kg/ha) and K (186.40 Kg/ha) in soil after harvest.
With recent technological advancements, the amount of personal user data that is being generated ... more With recent technological advancements, the amount of personal user data that is being generated is immense. Due to the large volume of data, machine learning algorithms such as neural networks are serving as the backbone to derive patterns from this data quickly. This need for big data analytics comes at the cost of the privacy of user data. The second challenge that must be solved relates to the scalability of the machine learning algorithm. Neural networks are known to deteriorate as the volume of the data increases due to complex sum and sigmoid calculations. Therefore in this thesis, an attempt to parallelize the neural network while also maintaining the privacy of user data is made. This model would provide a viable option for big data analytics without sacrificing the privacy of individual users while also maintaining precision and the classification accuracy of the model. The implementation of the parallelized privacy preserving neural network will be based on the MapReduce ...
Progress in Geography, 2021
资源科学, 2020
The relationship between economic growth and resource-environmental pressure is an important rese... more The relationship between economic growth and resource-environmental pressure is an important research area for regional sustainable development. However, few studies have systematically summarized relevant research results within different theoretical contexts, and few have compared the pros and cons of different theories, methods, models, indicators, and evaluation systems. Therefore, it is difficult to determine their theoretical contributions. This article reviewed five kinds of theoretical models and empirical studies in this field. It also compared their theoretical connotations, methods, evaluation systems, and applicability. In addition, this article proposed the basic framework and key content of analysis on the relationship between regional economic growth and resource-environmental pressures. We aimed to provide some theoretical reference and technical support for advancing related research. The results show that: (1) The theories, methods, and indicators of this field have different advantages, disadvantages, and application scope. Relevant research should be carried out under the basic framework of testing the direction of causality → identifying the EKC turning point/decoupling state → assessing the ecoefficiency intensity → determining the coupling coordination phase. Regional sustainable development policies should be developed based on the idea of"direction regulation → turning point/state regulation → intensity regulation → system regulation." (2) We should focus on method, model, and evaluation system optimization. Studies on discovering driving factors, mechanisms, and path selection should be emphasized. Furthermore, it is necessary to implement research on multi-case comparison, remote coupling, and theoretical association in the future.
International Journal of Pharmacy Research & Technology, 2019
FRED COOLBORThe development of resistance in many microorganisms to synthetic drugs, inability of... more FRED COOLBORThe development of resistance in many microorganisms to synthetic drugs, inability of these synthetic drugs to provide cure for some diseases and infections calls for serious attention. Due to this development, the antimicrobial activity, phytochemical analysis and antioxidant properties of aqueous and ethanol leaf extracts of Phyllanthus muellerianus was studied. Well in agar diffusion method was used for the antimicrobial test. Chemical methods was used for the preliminary phytochemical screening and the in vitro antioxidant tests. The aqueous leaf extract possessed higher antibacterial potency (14.0±0.16Â37.3±0.03 mm) on the test bacteria species and less of antifungal activity (5.0±0.2Â16.0±0.0 mm) than the antibacterial (6.2±0.5Â20.8±0.3 mm) and antifungal antibiotics (6.5±0.07-17.7±0.4 mm) respectively. Methanol extract only inhibited Candida krusei with inhibition of 4.5±0.3 mm. In both the qualitative and quantitative phytochemicals N AKHARAIYI* AND ABAYOMI OLABANJO DINA screened, aqueous extract was of higher yield thanmethanol extract. The in vitro antioxidant assay of the leaf extract in free radical scavenging (DPPH), ferric reducing antioxidant and hydroxyl radical scavenging properties yielded values able to represent antimicrobial potentials which may make the plant serve as alternative medicine for cure of the many microbial disease origins that plaque around properties was exhibited by P. muellerianus leaf extract, it may enhance a large segment of the world population to rely upon the use of traditional system of medicine for disease healing with novel plants. screened, aqueous extract was of higher yield than the world. As valuable antimicrobial potential, phytochemical constituents and in vitro antioxidant
International Journal of Advanced Research in Science, Communication and Technology, 2021
Medicaments, plants and plant-based are the basis of many of the modern pharmaceuticals we use to... more Medicaments, plants and plant-based are the basis of many of the modern pharmaceuticals we use today for our various purposes. The aim of the present study was to evaluate the antioxidant, phytochemical and antibacterial and antifungal activities of the Achyranthes aspera plant extract in different organic solvents. The radical scavenging activity of the different extracts of root, stem, leaf, and seed were evaluated by DPPH assay and the antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa, and antifungal activity against Fusarium sp. and Aspergillus nigerwas studied by Agar well cut diffusion method. All of the extracts exhibited different antioxidant and antibacterial activities and the activities varied from solvent to solvent, and the activities are concentrated. The antioxidant and antimicrobial activities were compared with the positive control Ascorbic acid and Cefuroxime. A qualitative phytochemical analysis was carried out and found to possess bi...
Chinese Journal of Integrative Medicine, 2013
International Journal of Current Microbiology and applied Sciences , 2022
Annals of Plant Sciences, 2022
As the microorganisms have developed the inherent ability to develop and adopt a mechanism of res... more As the microorganisms have developed the inherent ability to develop and adopt a mechanism of resistance against antibiotics. The harmful side effects of antibiotic including their cost of drug development have slowly shifted toward the plant derived phytochemical based medicines. The screening of antimicrobial property of this medicinal plant shows a positive result against the different species of bacteria (S. aureus, P. aeruginosa and fungi (A. Niger, Fusarium). This study of phytochemicals shows the important part to prevent and protect the plant against the microorganisms as well as phytochemicals to provide the information about the compound which are responsible for the antimicrobial activity like Alkaloids, Phenolics, and Flavonoids etc. This plant has enough amount of Total phenolics and hence, it has a high anti-oxidant activity. The anti-oxidant activity involve in the prevention of plant cell tissue damage. Anti-oxidant activity is measured by DPPH. The L. acidissima plant have many applications such as in bale diarrhea, dysentery, used for liver, cardiac tonic and effective for high cough, sore throat.
Medicaments, plants and plant-based are the basis of many of the modern pharmaceuticals we use to... more Medicaments, plants and plant-based are the basis of many of the modern pharmaceuticals we use today for our various purposes. The aim of the present study was to evaluate the antioxidant, phytochemical and antibacterial and antifungal activities of the Achyranthes aspera plant extract in different organic solvents. The radical scavenging activity of the different extracts of root, stem, leaf, and seed were evaluated by DPPH assay and the antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa, and antifungal activity against Fusarium sp. and Aspergillus niger was studied by Agar well cut diffusion method. All of the extracts exhibited different antioxidant and antibacterial activities and the activities varied from solvent to solvent, and the activities are concentrated. The antioxidant and antimicrobial activities were compared with the positive control Ascorbic acid and Cefuroxime. A qualitative phytochemical analysis was carried out and found to possess bioactive compounds like alkaloids, glycosides, terpenoids, steroids, flavonoids, tannins.
Natural Volatiles and Essential Oils, Nov 30, 2021
To determine the antioxidant, antimicrobial activity and phytochemical analysis of Psidium guajav... more To determine the antioxidant, antimicrobial activity and phytochemical analysis of Psidium guajava leaves and stems extracts against bacteria (Staphylococcus aureus and Pseudomonas aeruginosa) and fungi (Fusarium sp. and Aspergillus niger). The Guava leaves and stems were extracted by aqueous (hot and cold water) as well as solvent (methanol and n-hexane) extraction. The efficacy of these extracts was tested for antimicrobial activity against those bacteria and fungi through a welldiffusion method employing 50 µl leaf and stem extract solution per well. The quantitative analysis of phytochemical was done by using desire solvent; antioxidant activity was determined by 2, 2'-1-picrylhyrazyl (DPPH) radical scavenging method. According to the findings of above assays, all extracts of the guava leaves and stems showed inhibitory activity against such microorganisms. The hot extract of leaves had an antibacterial activity with mean zoon of inhibition of 13 mm. Cold extract of leaves had 4 mm inhibition whereas, absence of inhibition in stems' cold extraction, against Staphylococcus aureus and Pseudomonas aeruginosa. The phytochemical analysis saw the presence of alkaloids, flavonoids, steroids, terpenoids, tannins, phenolics. This plant contains the high amount of phenolics. This study provides scientific understanding to further determine the phytochemicals, antioxidant and antimicrobial values, investigate other pharmacological properties.
International Conference on Researches in Science and Technology (ICRST-21), 2021
Bacteriocins are small ribosomally synthesized antimicrobial peptides, that is produced by some m... more Bacteriocins are small ribosomally synthesized antimicrobial peptides, that is produced by some microorganisms
including lactic acid bacteria (LAB), a group of Gram-positive bacteria (cocci, rods) expressing high tolerance for wide range of
pH. The bacteriocin shows broad range of thermo stability from 37°C to 121°C for 30 minutes. The well diffusion assay showed the
bacteriocin produced by LAB is inhibiting the growth of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and
Bacillus subtilis presenting a wide spectrum of inhibition. The molecular weight of bacteriocin is 6 kDa and it was determined by
SDS-PAGE. This bacteriocins kill bacteria rapidly and are biologically active at very low concentrations. There is many
bacteriocins have been investigated with respect to their potential use in promoting human, plant, and animal health, and also, as
food biopreservatives. Bacteriocins produced by LAB are particularly interesting since several LAB have been granted as GRAS
(Generally Recognized as Safe) status from FDA. Because it is not always possible to extract active bacteriocins secreted from cells
grown in liquid medium, we developed a simple and inexpensive extraction procedure, using ammonium sulphate precipitation and
DEAE Sephadex A-25 Column. By using Folin’s Lowery method, the concentration can be finding out. I hereby present a detailed
procedure that leads to the rapid extraction of secreted bioactive bacteriocin peptides from the Lactic acid bacteria, isolated from
curd sample. Also present a simple method for the optimization of bacteriocin for their detection of activity from the purified
bacteriocin sample.
International Conference on Researches in Science and Technology (ICRST-21), 2021
From the isolated species of LAB (Lactic Acid Bacteria) shows pronounced antibacterial activity b... more From the isolated species of LAB (Lactic Acid Bacteria) shows pronounced antibacterial activity between the initial
logarithmic and stationary phase. The most enormous activity in the composed medium was achieved at pH of 7, incubation
temperature 37°C and incubation period of 48 h under static condition. Supplementation of nutrients demonstrated that the huge
quantities of bacteriocin and could be produced by addition of glucose (2%) manipulating the dextrose (2%) and NaCl (0.5%-
2.0%). Since it displayed the increase in the activity from 981.25 AU/mL to 1139.74 AU/mL. The compound produced by LAB was
wholly/partially inactivated by some of the proteolytic enzymes, which confirms their proteinaceous nature. The activity of
bacteriocin producing, the organism is not dependent on the glycosylation because the antimicrobial activity of bacteriocin was
unaffected by the α-amylase and other enzymes. The bacteriocin shows broad range of thermo stability from 37°C to 121°C for 30
minutes. The Folin’s Lowery method describes their concentration. The well diffusion assay showed the bacteriocin produced by
LAB is inhibiting the growth of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis presenting
a wide spectrum of inhibition. The antimicrobial activity of the proteinaceous compound namely bacteriocin or BLIS (Bacteriocin
like inhibitory substance) produced by Lactobacilli isolated in this work are largely identified and have been found to have
potential antimicrobial activities toward closely related bacteria and undesirable harmful microorganisms. On this account, they
are helpful in the field of food preservation/safety or pharmaceutical applications and healthcare.
International Journal of Current Microbiology and Applied Sciences, 2021
International Journal of Science, Engineering and Management , 2021
Bacteriocins are small ribosomally synthesized antimicrobial peptides, that is produced by some m... more Bacteriocins are small ribosomally synthesized antimicrobial peptides, that is produced by some microorganisms including lactic acid bacteria (LAB), a group of Gram-positive bacteria (cocci, rods) expressing high tolerance for wide range of pH. The bacteriocin shows broad range of thermo stability from 37°C to 121°C for 30 minutes. The well diffusion assay showed the bacteriocin produced by LAB is inhibiting the growth of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis presenting a wide spectrum of inhibition. The molecular weight of bacteriocin is 6 kDa and it was determined by SDS-PAGE. This bacteriocins kill bacteria rapidly and are biologically active at very low concentrations. There is many bacteriocins have been investigated with respect to their potential use in promoting human, plant, and animal health, and also, as food biopreservatives. Bacteriocins produced by LAB are particularly interesting since several LAB have been granted as GRAS (Generally Recognized as Safe) status from FDA. Because it is not always possible to extract active bacteriocins secreted from cells grown in liquid medium, we developed a simple and inexpensive extraction procedure, using ammonium sulphate precipitation and DEAE Sephadex A-25 Column. By using Folin's Lowery method, the concentration can be finding out. I hereby present a detailed procedure that leads to the rapid extraction of secreted bioactive bacteriocin peptides from the Lactic acid bacteria, isolated from curd sample. Also present a simple method for the optimization of bacteriocin for their detection of activity from the purified bacteriocin sample.
International Journal of Science, Engineering and Management (IJSEM), 2021
From the isolated species of LAB (Lactic Acid Bacteria) shows pronounced antibacterial activity b... more From the isolated species of LAB (Lactic Acid Bacteria) shows pronounced antibacterial activity between the initial logarithmic and stationary phase. The most enormous activity in the composed medium was achieved at pH of 7, incubation temperature 37°C and incubation period of 48 h under static condition. Supplementation of nutrients demonstrated that the huge quantities of bacteriocin and could be produced by addition of glucose (2%) manipulating the dextrose (2%) and NaCl (0.5%-2.0%). Since it displayed the increase in the activity from 981.25 AU/mL to 1139.74 AU/mL. The compound produced by LAB was wholly/partially inactivated by some of the proteolytic enzymes, which confirms their proteinaceous nature. The activity of bacteriocin producing, the organism is not dependent on the glycosylation because the antimicrobial activity of bacteriocin was unaffected by the α-amylase and other enzymes. The bacteriocin shows broad range of thermo stability from 37°C to 121°C for 30 minutes. The Folin's Lowery method describes their concentration. The well diffusion assay showed the bacteriocin produced by LAB is inhibiting the growth of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis presenting a wide spectrum of inhibition. The antimicrobial activity of the proteinaceous compound namely bacteriocin or BLIS (Bacteriocin like inhibitory substance) produced by Lactobacilli isolated in this work are largely identified and have been found to have potential antimicrobial activities toward closely related bacteria and undesirable harmful microorganisms. On this account, they are helpful in the field of food preservation/safety or pharmaceutical applications and healthcare.
Uploads
Papers by Dipeshkumar Patel
including lactic acid bacteria (LAB), a group of Gram-positive bacteria (cocci, rods) expressing high tolerance for wide range of
pH. The bacteriocin shows broad range of thermo stability from 37°C to 121°C for 30 minutes. The well diffusion assay showed the
bacteriocin produced by LAB is inhibiting the growth of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and
Bacillus subtilis presenting a wide spectrum of inhibition. The molecular weight of bacteriocin is 6 kDa and it was determined by
SDS-PAGE. This bacteriocins kill bacteria rapidly and are biologically active at very low concentrations. There is many
bacteriocins have been investigated with respect to their potential use in promoting human, plant, and animal health, and also, as
food biopreservatives. Bacteriocins produced by LAB are particularly interesting since several LAB have been granted as GRAS
(Generally Recognized as Safe) status from FDA. Because it is not always possible to extract active bacteriocins secreted from cells
grown in liquid medium, we developed a simple and inexpensive extraction procedure, using ammonium sulphate precipitation and
DEAE Sephadex A-25 Column. By using Folin’s Lowery method, the concentration can be finding out. I hereby present a detailed
procedure that leads to the rapid extraction of secreted bioactive bacteriocin peptides from the Lactic acid bacteria, isolated from
curd sample. Also present a simple method for the optimization of bacteriocin for their detection of activity from the purified
bacteriocin sample.
logarithmic and stationary phase. The most enormous activity in the composed medium was achieved at pH of 7, incubation
temperature 37°C and incubation period of 48 h under static condition. Supplementation of nutrients demonstrated that the huge
quantities of bacteriocin and could be produced by addition of glucose (2%) manipulating the dextrose (2%) and NaCl (0.5%-
2.0%). Since it displayed the increase in the activity from 981.25 AU/mL to 1139.74 AU/mL. The compound produced by LAB was
wholly/partially inactivated by some of the proteolytic enzymes, which confirms their proteinaceous nature. The activity of
bacteriocin producing, the organism is not dependent on the glycosylation because the antimicrobial activity of bacteriocin was
unaffected by the α-amylase and other enzymes. The bacteriocin shows broad range of thermo stability from 37°C to 121°C for 30
minutes. The Folin’s Lowery method describes their concentration. The well diffusion assay showed the bacteriocin produced by
LAB is inhibiting the growth of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis presenting
a wide spectrum of inhibition. The antimicrobial activity of the proteinaceous compound namely bacteriocin or BLIS (Bacteriocin
like inhibitory substance) produced by Lactobacilli isolated in this work are largely identified and have been found to have
potential antimicrobial activities toward closely related bacteria and undesirable harmful microorganisms. On this account, they
are helpful in the field of food preservation/safety or pharmaceutical applications and healthcare.
including lactic acid bacteria (LAB), a group of Gram-positive bacteria (cocci, rods) expressing high tolerance for wide range of
pH. The bacteriocin shows broad range of thermo stability from 37°C to 121°C for 30 minutes. The well diffusion assay showed the
bacteriocin produced by LAB is inhibiting the growth of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and
Bacillus subtilis presenting a wide spectrum of inhibition. The molecular weight of bacteriocin is 6 kDa and it was determined by
SDS-PAGE. This bacteriocins kill bacteria rapidly and are biologically active at very low concentrations. There is many
bacteriocins have been investigated with respect to their potential use in promoting human, plant, and animal health, and also, as
food biopreservatives. Bacteriocins produced by LAB are particularly interesting since several LAB have been granted as GRAS
(Generally Recognized as Safe) status from FDA. Because it is not always possible to extract active bacteriocins secreted from cells
grown in liquid medium, we developed a simple and inexpensive extraction procedure, using ammonium sulphate precipitation and
DEAE Sephadex A-25 Column. By using Folin’s Lowery method, the concentration can be finding out. I hereby present a detailed
procedure that leads to the rapid extraction of secreted bioactive bacteriocin peptides from the Lactic acid bacteria, isolated from
curd sample. Also present a simple method for the optimization of bacteriocin for their detection of activity from the purified
bacteriocin sample.
logarithmic and stationary phase. The most enormous activity in the composed medium was achieved at pH of 7, incubation
temperature 37°C and incubation period of 48 h under static condition. Supplementation of nutrients demonstrated that the huge
quantities of bacteriocin and could be produced by addition of glucose (2%) manipulating the dextrose (2%) and NaCl (0.5%-
2.0%). Since it displayed the increase in the activity from 981.25 AU/mL to 1139.74 AU/mL. The compound produced by LAB was
wholly/partially inactivated by some of the proteolytic enzymes, which confirms their proteinaceous nature. The activity of
bacteriocin producing, the organism is not dependent on the glycosylation because the antimicrobial activity of bacteriocin was
unaffected by the α-amylase and other enzymes. The bacteriocin shows broad range of thermo stability from 37°C to 121°C for 30
minutes. The Folin’s Lowery method describes their concentration. The well diffusion assay showed the bacteriocin produced by
LAB is inhibiting the growth of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis presenting
a wide spectrum of inhibition. The antimicrobial activity of the proteinaceous compound namely bacteriocin or BLIS (Bacteriocin
like inhibitory substance) produced by Lactobacilli isolated in this work are largely identified and have been found to have
potential antimicrobial activities toward closely related bacteria and undesirable harmful microorganisms. On this account, they
are helpful in the field of food preservation/safety or pharmaceutical applications and healthcare.