The aim of the present work was to investigate the phytochemical components of an antidiabetic p... more The aim of the present work was to investigate the phytochemical components of an antidiabetic polyherbal formulation. The formulation was prepared using ingredients of Azadirachta indica A. Juss, Commiphora mukul, Curcuma longa L, Emblica officinalis Gaertn, Garcinia cambogia Gaertn, Gymnema sylvestre Retz, Momordica charantia L, Ocimum sanctum L and Trigonella foenum-graecum L. The formulation was evaluated for phytochemical properties by following standard protocols. The colour intensity or the precipitate formation was used as analytical responses to these tests. The formulation revealed the presence of active phytoconstituents such as carbohydrates, amino acid, proteins, phenols, flavonoids, tannins, terpenoids, steroids and insulin. The HPTLC fingerprint analysis revealed sixteen bioactive compounds when scanned at 520 nm and thirteen bioactive compounds at 254 nm. The study confirms that the formulation contains many phytoconstituents making it a prospective candidate for further development
The aim of this study was to investigate the phytochemical profile, heavy metal contents and anti... more The aim of this study was to investigate the phytochemical profile, heavy metal contents and antioxidant activities of an antidiabetic polyherbal formulation (PHF) containing Azadirachta indicaA. Juss, Commiphora mukul, Curcuma longaL, Emblica officinalisGaertn, Garcinia cambogiaGaertn, Gymnema sylvestreRetz, Momordica charantiaL, Ocimum sanctumL and Trigonella foenum-graecum L. Total phenolic content, flavonoids and flavonols present in the formulation were determined by standard methods. Antioxidant potential was evaluated using total antioxidant activity, ferric reducing antioxidant power and 2, 2-diphenylpicrylhydrazyl(DPPH) assays. The chemical composition of the formulation was studied using Gas Chromatography–Mass Spectrometry (GC-MS). Heavy metals were analysed using Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES). The total phenolic content of the PHF was 232.2 mg gallic acid equivalent/g. Total flavonols and total flavonoid contents of the PHF were 180.7 and 135.3 mg quercetin equivalent/g, respectively. The PHF showed a reducing power of 26.8 mg ascorbic acid equivalent/g. Total antioxidant capacity was found to be 144.4 mg ascorbic acid equivalent/g. The ferric reducing antioxidant power (FRAP) of the PHF was 1260.9 (μM Fe (II)/g). The DPPH radical scavenging activity expressed as IC50value was 2.6 µg/mL for gallic acid and 3.1 µg/mL for the formulation. The levelsof heavy metals, such as arsenic, cadmium, chromium, lead and mercury in the PHF were within the World Health Organization (WHO) limits. Out of the eight bioactive compounds identified inthe formulation, the major compounds were 10-Octadecenoic acid methyl ester, Octadecanoic acid methyl ester and Hexadecanoic acid methyl ester. The results show that the polyherbal formulation possesses good antioxidant activity and could be a potential candidate for antidiabetic studies.
The aim of the present work was to investigate the phytochemical components of an antidiabetic p... more The aim of the present work was to investigate the phytochemical components of an antidiabetic polyherbal formulation. The formulation was prepared using ingredients of Azadirachta indica A. Juss, Commiphora mukul, Curcuma longa L, Emblica officinalis Gaertn, Garcinia cambogia Gaertn, Gymnema sylvestre Retz, Momordica charantia L, Ocimum sanctum L and Trigonella foenum-graecum L. The formulation was evaluated for phytochemical properties by following standard protocols. The colour intensity or the precipitate formation was used as analytical responses to these tests. The formulation revealed the presence of active phytoconstituents such as carbohydrates, amino acid, proteins, phenols, flavonoids, tannins, terpenoids, steroids and insulin. The HPTLC fingerprint analysis revealed sixteen bioactive compounds when scanned at 520 nm and thirteen bioactive compounds at 254 nm. The study confirms that the formulation contains many phytoconstituents making it a prospective candidate for further development
The aim of this study was to investigate the phytochemical profile, heavy metal contents and anti... more The aim of this study was to investigate the phytochemical profile, heavy metal contents and antioxidant activities of an antidiabetic polyherbal formulation (PHF) containing Azadirachta indicaA. Juss, Commiphora mukul, Curcuma longaL, Emblica officinalisGaertn, Garcinia cambogiaGaertn, Gymnema sylvestreRetz, Momordica charantiaL, Ocimum sanctumL and Trigonella foenum-graecum L. Total phenolic content, flavonoids and flavonols present in the formulation were determined by standard methods. Antioxidant potential was evaluated using total antioxidant activity, ferric reducing antioxidant power and 2, 2-diphenylpicrylhydrazyl(DPPH) assays. The chemical composition of the formulation was studied using Gas Chromatography–Mass Spectrometry (GC-MS). Heavy metals were analysed using Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES). The total phenolic content of the PHF was 232.2 mg gallic acid equivalent/g. Total flavonols and total flavonoid contents of the PHF were 180.7 and 135.3 mg quercetin equivalent/g, respectively. The PHF showed a reducing power of 26.8 mg ascorbic acid equivalent/g. Total antioxidant capacity was found to be 144.4 mg ascorbic acid equivalent/g. The ferric reducing antioxidant power (FRAP) of the PHF was 1260.9 (μM Fe (II)/g). The DPPH radical scavenging activity expressed as IC50value was 2.6 µg/mL for gallic acid and 3.1 µg/mL for the formulation. The levelsof heavy metals, such as arsenic, cadmium, chromium, lead and mercury in the PHF were within the World Health Organization (WHO) limits. Out of the eight bioactive compounds identified inthe formulation, the major compounds were 10-Octadecenoic acid methyl ester, Octadecanoic acid methyl ester and Hexadecanoic acid methyl ester. The results show that the polyherbal formulation possesses good antioxidant activity and could be a potential candidate for antidiabetic studies.
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Papers by Pawan Kumar
activities of an antidiabetic polyherbal formulation (PHF) containing Azadirachta indicaA. Juss,
Commiphora mukul, Curcuma longaL, Emblica officinalisGaertn, Garcinia cambogiaGaertn,
Gymnema sylvestreRetz, Momordica charantiaL, Ocimum sanctumL and Trigonella foenum-graecum
L. Total phenolic content, flavonoids and flavonols present in the formulation were determined by
standard methods. Antioxidant potential was evaluated using total antioxidant activity, ferric reducing
antioxidant power and 2, 2-diphenylpicrylhydrazyl(DPPH) assays. The chemical composition of the
formulation was studied using Gas Chromatography–Mass Spectrometry (GC-MS). Heavy metals were
analysed using Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES). The total
phenolic content of the PHF was 232.2 mg gallic acid equivalent/g. Total flavonols and total flavonoid
contents of the PHF were 180.7 and 135.3 mg quercetin equivalent/g, respectively. The PHF showed a
reducing power of 26.8 mg ascorbic acid equivalent/g. Total antioxidant capacity was found to be 144.4
mg ascorbic acid equivalent/g. The ferric reducing antioxidant power (FRAP) of the PHF was 1260.9
(μM Fe (II)/g). The DPPH radical scavenging activity expressed as IC50value was 2.6 µg/mL for gallic
acid and 3.1 µg/mL for the formulation. The levelsof heavy metals, such as arsenic, cadmium,
chromium, lead and mercury in the PHF were within the World Health Organization (WHO) limits. Out
of the eight bioactive compounds identified inthe formulation, the major compounds were 10-Octadecenoic acid methyl ester, Octadecanoic acid methyl ester and Hexadecanoic acid methyl ester. The
results show that the polyherbal formulation possesses good antioxidant activity and could be a potential
candidate for antidiabetic studies.
activities of an antidiabetic polyherbal formulation (PHF) containing Azadirachta indicaA. Juss,
Commiphora mukul, Curcuma longaL, Emblica officinalisGaertn, Garcinia cambogiaGaertn,
Gymnema sylvestreRetz, Momordica charantiaL, Ocimum sanctumL and Trigonella foenum-graecum
L. Total phenolic content, flavonoids and flavonols present in the formulation were determined by
standard methods. Antioxidant potential was evaluated using total antioxidant activity, ferric reducing
antioxidant power and 2, 2-diphenylpicrylhydrazyl(DPPH) assays. The chemical composition of the
formulation was studied using Gas Chromatography–Mass Spectrometry (GC-MS). Heavy metals were
analysed using Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES). The total
phenolic content of the PHF was 232.2 mg gallic acid equivalent/g. Total flavonols and total flavonoid
contents of the PHF were 180.7 and 135.3 mg quercetin equivalent/g, respectively. The PHF showed a
reducing power of 26.8 mg ascorbic acid equivalent/g. Total antioxidant capacity was found to be 144.4
mg ascorbic acid equivalent/g. The ferric reducing antioxidant power (FRAP) of the PHF was 1260.9
(μM Fe (II)/g). The DPPH radical scavenging activity expressed as IC50value was 2.6 µg/mL for gallic
acid and 3.1 µg/mL for the formulation. The levelsof heavy metals, such as arsenic, cadmium,
chromium, lead and mercury in the PHF were within the World Health Organization (WHO) limits. Out
of the eight bioactive compounds identified inthe formulation, the major compounds were 10-Octadecenoic acid methyl ester, Octadecanoic acid methyl ester and Hexadecanoic acid methyl ester. The
results show that the polyherbal formulation possesses good antioxidant activity and could be a potential
candidate for antidiabetic studies.