pegRNA designer

Knock a tag into the C-terminus of a human gene, by prime editing. You supply the target (a genome window, the insert site and the strand) and the cargo to insert. The pipeline designs the guides for you, with PRIDICT2 proposing the pegRNA spacer, primer binding site and reverse transcriptase template.

Every candidate then passes a cascade of checks: off-target search, recoding and microhomology logic, cryptic splice sites, splicing prediction, structural importance from AlphaFold-DB, RNA secondary structure and docking. The first candidate that passes every check is the design.

Run it as a fast dry run with stand-in scorers, or with the real models. For heterozygous cells, supply phased SNPs to ask for an allele-specific design. Designs are engineering previews, so validate them at the bench before ordering. This page needs JavaScript to open the design form.

Built by Mrinal Joshi.