We tested the feasibility and efficacy of a novel high-dose sequential chemoradiotherapy programm... more We tested the feasibility and efficacy of a novel high-dose sequential chemoradiotherapy programme (HDS) in 14 relapsed or refractory non-Hodgkin's lymphoma patients with very poor prognostic features, i.e. transformed histology, marrow invasion, low performance status. This regimen included the sequential administration of high-dose cyclophosphamide (CY) 7 g/m2 followed by high-dose methotrexate (MTX) 8 g/m2 and high-dose VP16 2 g/m2 and finally by total body irradiation (TBI)-melphalan and autograft of bone marrow and peripheral blood progenitor cells. No hemopoietic growth factor support was employed in any phase. There was one treatment-related death during the high-dose phase; three other patients did not complete the programme. All 10 patients concluding the programme achieved complete remission, with four patients in complete clinical remission at a median follow up of 34 months. Median overall survival was 27 months and median failure-free survival (FFS) was 12 months. T...
Bollettino della Società italiana di biologia sperimentale, Jan 30, 1982
Blast cells from peripheral blood of 32 patients with acute leukemia were tested for their abilit... more Blast cells from peripheral blood of 32 patients with acute leukemia were tested for their ability to react with a monoclonal antibody (D1.B6) specific for HLA-DR surface antigen. In order to evaluate the degree of leukemic cell differentiation a monoclonal antibody (R1.B19) specific for the granulocytopoietic lineage was also employed. The results demonstrated that a considerable proportion of blast cell populations expressed the HLA-DR antigen, while only a small fraction of cells expressed the myeloid antigen.
161 cases of CML have been studied. Clinically significant adenopathies were present in 3,2% of t... more 161 cases of CML have been studied. Clinically significant adenopathies were present in 3,2% of the patients at the moment of diagnosis, and in the subsequent course they appeared in 7% of them. The behaviour of adenopathies showed to be unrelated to: --splenomegaly; --blastic metamorphosis in the peripheral blood or in the marrow (which they often preceded from 3 to 26 months); --hematological sensibility to cytostatic therapy; and furthermore they often acted as the most important clinical and therapeutic problem. From the cyto-histological point of view three features have been observed: 1) blastic metamorphosis in a lymphnode showing features of myeloid metaplasia; 2) blastic invasion in a lymphnode without any sign of myeloid metaplasia; 3) malignant lymphoma. Cytological examination of imprints and ultrastructural studies, besides the usual histological investigations, proved to be useful for the definition of the above mentioned features. In lymphomatous forms, together with ...
The expression of HLA class I- like molecules was analyzed on human acute and chronic leukemic ce... more The expression of HLA class I- like molecules was analyzed on human acute and chronic leukemic cells. The presence on leukemic cells of class I- like molecules, absent on the patient's normal lymphocytes, was examined by complement- dependent lymphocytotoxicity using platelet absorbed alloantisera that recognize HLA-linked, 45-12 kd, beta-2-microglobulin associated molecules, selectively expressed on PHA-activated cells. A positive reactivity of the anti- class I- like alloantisera was found in 50% of the acute leukemias (cALL, T-ALL and AML), independently of the lineage of differentiation, while chronic lymphocytic leukemias (B-CLL) were constantly negative. It is suggested that beta-2-microglobulin associated HLA molecules may represent markers of leukemic blast activation and/or maturation state.
Questionnaire ; THI ; Handicap ; Qualité de vie Résumé Notre adaptation française du Tinnitus Han... more Questionnaire ; THI ; Handicap ; Qualité de vie Résumé Notre adaptation française du Tinnitus Handicap Inventory (THIf) a été validée sur 174 patients âgés de 54,2 ± 16,4 ans, présentant des acouphènes chroniques comme motif principal de consultation. Le coefficient ␣ de Cronbach a montré une consistance interne de 0,90 pour l'ensemble de THIf et respectivement de 0,85, 0,80 et 0,49 pour les sous-échelles émotionnelle, fonctionnelle et catastrophique. Les corrélations items/total ont été significatives (p < 0,05) et ont varié entre 0,21 (t = 3,08) et 0,71 (t = 12,43). Des corrélations significatives (p < 0,001) ont également été retrouvées entre le score total du THIf et les sous-échelles fonctionnelles (r = 0,91 ; t = 29,72), émotionnelles (r = 0,90 ; t = 27,37) et catastrophiques (r = 0,69 ; t = 12,54). Les scores du THIf ont été corrélés significativement (p < 0,001) avec ceux des échelles d'autoévaluation de la sévérité de l'acouphène et de son impact sur la qualité de vie des patients. Ainsi, le THIf a une excellente fiabilité interne, équivalente à celle de la version originale. Il est robuste du point de vue psychométrique, compréhensible, de passation facile et rapide. Le THIf peut donc être utilisé pour la prise en charge des patients francophones souffrant d'acouphènes, car il permet une quantification des composants psycho-émotionnels et fonctionnels de l'handicap lié à l'acouphène. Il peut également servir d'outil d'évaluation de l'efficacité d'une thérapie et contribuera ainsi à la standardisation des méthodes d'évaluation des acouphènes à l'échelle mondiale.
We tested the feasibility and efficacy of a novel high-dose sequential chemoradiotherapy programm... more We tested the feasibility and efficacy of a novel high-dose sequential chemoradiotherapy programme (HDS) in 14 relapsed or refractory non-Hodgkin's lymphoma patients with very poor prognostic features, i.e. transformed histology, marrow invasion, low performance status. This regimen included the sequential administration of high-dose cyclophosphamide (CY) 7 g/m2 followed by high-dose methotrexate (MTX) 8 g/m2 and high-dose VP16 2 g/m2 and finally by total body irradiation (TBI)-melphalan and autograft of bone marrow and peripheral blood progenitor cells. No hemopoietic growth factor support was employed in any phase. There was one treatment-related death during the high-dose phase; three other patients did not complete the programme. All 10 patients concluding the programme achieved complete remission, with four patients in complete clinical remission at a median follow up of 34 months. Median overall survival was 27 months and median failure-free survival (FFS) was 12 months. T...
Bollettino della Società italiana di biologia sperimentale, Jan 30, 1982
Blast cells from peripheral blood of 32 patients with acute leukemia were tested for their abilit... more Blast cells from peripheral blood of 32 patients with acute leukemia were tested for their ability to react with a monoclonal antibody (D1.B6) specific for HLA-DR surface antigen. In order to evaluate the degree of leukemic cell differentiation a monoclonal antibody (R1.B19) specific for the granulocytopoietic lineage was also employed. The results demonstrated that a considerable proportion of blast cell populations expressed the HLA-DR antigen, while only a small fraction of cells expressed the myeloid antigen.
161 cases of CML have been studied. Clinically significant adenopathies were present in 3,2% of t... more 161 cases of CML have been studied. Clinically significant adenopathies were present in 3,2% of the patients at the moment of diagnosis, and in the subsequent course they appeared in 7% of them. The behaviour of adenopathies showed to be unrelated to: --splenomegaly; --blastic metamorphosis in the peripheral blood or in the marrow (which they often preceded from 3 to 26 months); --hematological sensibility to cytostatic therapy; and furthermore they often acted as the most important clinical and therapeutic problem. From the cyto-histological point of view three features have been observed: 1) blastic metamorphosis in a lymphnode showing features of myeloid metaplasia; 2) blastic invasion in a lymphnode without any sign of myeloid metaplasia; 3) malignant lymphoma. Cytological examination of imprints and ultrastructural studies, besides the usual histological investigations, proved to be useful for the definition of the above mentioned features. In lymphomatous forms, together with ...
The expression of HLA class I- like molecules was analyzed on human acute and chronic leukemic ce... more The expression of HLA class I- like molecules was analyzed on human acute and chronic leukemic cells. The presence on leukemic cells of class I- like molecules, absent on the patient&#39;s normal lymphocytes, was examined by complement- dependent lymphocytotoxicity using platelet absorbed alloantisera that recognize HLA-linked, 45-12 kd, beta-2-microglobulin associated molecules, selectively expressed on PHA-activated cells. A positive reactivity of the anti- class I- like alloantisera was found in 50% of the acute leukemias (cALL, T-ALL and AML), independently of the lineage of differentiation, while chronic lymphocytic leukemias (B-CLL) were constantly negative. It is suggested that beta-2-microglobulin associated HLA molecules may represent markers of leukemic blast activation and/or maturation state.
Questionnaire ; THI ; Handicap ; Qualité de vie Résumé Notre adaptation française du Tinnitus Han... more Questionnaire ; THI ; Handicap ; Qualité de vie Résumé Notre adaptation française du Tinnitus Handicap Inventory (THIf) a été validée sur 174 patients âgés de 54,2 ± 16,4 ans, présentant des acouphènes chroniques comme motif principal de consultation. Le coefficient ␣ de Cronbach a montré une consistance interne de 0,90 pour l'ensemble de THIf et respectivement de 0,85, 0,80 et 0,49 pour les sous-échelles émotionnelle, fonctionnelle et catastrophique. Les corrélations items/total ont été significatives (p < 0,05) et ont varié entre 0,21 (t = 3,08) et 0,71 (t = 12,43). Des corrélations significatives (p < 0,001) ont également été retrouvées entre le score total du THIf et les sous-échelles fonctionnelles (r = 0,91 ; t = 29,72), émotionnelles (r = 0,90 ; t = 27,37) et catastrophiques (r = 0,69 ; t = 12,54). Les scores du THIf ont été corrélés significativement (p < 0,001) avec ceux des échelles d'autoévaluation de la sévérité de l'acouphène et de son impact sur la qualité de vie des patients. Ainsi, le THIf a une excellente fiabilité interne, équivalente à celle de la version originale. Il est robuste du point de vue psychométrique, compréhensible, de passation facile et rapide. Le THIf peut donc être utilisé pour la prise en charge des patients francophones souffrant d'acouphènes, car il permet une quantification des composants psycho-émotionnels et fonctionnels de l'handicap lié à l'acouphène. Il peut également servir d'outil d'évaluation de l'efficacité d'une thérapie et contribuera ainsi à la standardisation des méthodes d'évaluation des acouphènes à l'échelle mondiale.
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