Papers by Laxmipriya Padhi
Background: Recent interest in plant-based medicine is justified, as synthetic antioxidants and a... more Background: Recent interest in plant-based medicine is justified, as synthetic antioxidants and antibiotics suffer from several drawbacks. Plant extracts have been shown to possess health promoting properties, and they exert no selective physiological pressure on the pathogens that can result in development of drug resistance. The aim of this study was to investigate the antibacterial activity of Eleutherine bulbosa plant extracts against a series of multidrug-resistant (MDR) bacteria by calculating zones of inhibition, minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs) and timeekill kinetics. Methods: Dried bulb powder of E. bulbosa was extracted with five solvents (ethyl acetate, chloroform, butanol, ethanol and water) to select the best extractant for subsequent isolation of bioactive compounds to use against a battery of MDR bacteria. Results: Butanol was found to be the best extractant with an MIC ranging from 46 mg/mL to 187 mg/mL. Butanol and aqueous extracts showed higher zones of inhibition in comparison with standard antibiotics gentamicin and ciprofloxacin. Thin layer chromatography (TLC)-bioautography was carried out with the butanol extract against Staphylococcus aureus and Shigella boydii. A synergistic effect was established against S. boydii by combining the chloroform extract with gentamicin. Conclusion: The bulb extracts of E. bulbosa exhibited significant antibacterial activity. Further study is necessary to characterize the antibacterial compounds of this plant for use in clinical applications.
The global burden of bacterial infections is very high and has been exacerbated by increasing res... more The global burden of bacterial infections is very high and has been exacerbated by increasing resistance to multiple antibiotics. Antibiotic resistance leads to failed treatment of infections, which can ultimately lead to death. To overcome antibiotic resistance, it is necessary to identify new antibacterial agents. In this study, a total of 662 plant extracts (diverse parts) from 222 plant species (82 families, 177 genera) were screened for antibacterial activity using the agar cup plate method. The aqueous and methanolic extracts were prepared from diverse plant parts and screened against eight bacterial (two Gram-positive and six Gram-negative) species, most of which are involved in common infections with multiple antibiotic resistance. The methanolic extracts of several plants were shown to have zones of inhibition ě 12 mm against both Gram-positive and Gram-negative bacteria. The minimum inhibitory concentration was calculated only with methanolic extracts of selected plants, those showed zone of inhibition ě 12 mm against both Gram-positive and Gram-negative bacteria. Several extracts had minimum inhibitory concentration ď 1 mg/mL. Vitex negundo were shown to have significant antimicrobial activity. The species listed here were shown to have anti-infective activity against both Gram-positive and Gram-negative bacteria. These results may serve as a guide for selecting plant species that could yield the highest probability of finding promising compounds responsible for the antibacterial activities against a broad spectrum of bacterial species. Further investigation of the phytochemicals from these plants will help to identify the lead compounds for drug discovery.
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Papers by Laxmipriya Padhi