Paper, pulp and plywood industries of north Indian states viz. Uttarakhand, Haryana and Punjab he... more Paper, pulp and plywood industries of north Indian states viz. Uttarakhand, Haryana and Punjab heavily depend on Eucalyptus plantations for raw material. However, Cylindrocladium leaf and seedling blight cause large scale mortality in nurseries thus jeopardizing plantation programs. In the present study 71 isolates of the fungal pathogen Cylindrocladium quinqueseptatum collected from three north Indian states were screened for their relative virulence. Representative pathogen isolates were screened on different culture media for variability in cultural and micro-morphological traits.
There is heavy biotic pressure on the Himalayan alpine meadows of Uttarakhand in India for the ex... more There is heavy biotic pressure on the Himalayan alpine meadows of Uttarakhand in India for the extraction of Cordyceps sinensis, a medicinally important fungus. Molecular characterization of Cordyceps sinensis germplasm of Indian origin has not yet been established. In the current study isolates of C. sinensis collected from different parts of Uttarakhand have been analyzed by Random Amplified Polymorphic DNA for assessing genetic diversity among the isolates.
Mohanty PS, Harsh NSK, Pandey A. 2011 -First report of Ganoderma resinaceum and G. weberianum fro... more Mohanty PS, Harsh NSK, Pandey A. 2011 -First report of Ganoderma resinaceum and G. weberianum from north India based on ITS sequence analysis and micromorphology. Mycosphere 2(4), 469-474.
We developed PCR primers to detect Cylindrocladium quinqueseptatum which infect Eucalyptus causin... more We developed PCR primers to detect Cylindrocladium quinqueseptatum which infect Eucalyptus causing leaf and seedling blight resulting in heavy seedling mortality in North Indian states. Primers based on sequence analysis of internal transcribed spacer region 1 and 5.8S of ribosomal DNA produced PCR product of 245bp. The internal transcribed spacer (ITS) of the ribosomal DNA (rDNA) sub unit repeat was sequenced in 26 isolates of Cylindrocladium quinqueseptatum and sequences were aligned and compared with the ITS sequences of other fungi in GenBank. No amplification resulted from PCR reactions on fungal DNA from 6 common forest fungi, 10 soil contaminates and 6 Eucalyptus pathogens. For amplifications directly from infected tissues, a nested primer PCR using two rounds of amplification was done. First, the entire ITS was amplified with universal fungal primer; a second round of amplification was carried out with species specific primer that amplified a 245 bp PCR product. The method detected leaf and seedling blight in artificially and naturally infected Eucalyptus plants. From the soil also the pathogen was detected using species specific primer. In sampling studies, C. quinqueseptatum was detected by PCR from artificially infected seedlings at 6 days post inoculation, before any visible symptoms were present. The PCR assay and direct tissue extraction methods provide tools which may be used to detect C. quinqueseptatum from soil, plant cuttings and adjoining Eucalyptus plantations serving as recurring source of infection and thus limit the transmission and spread of new aggressive strains of C. quinqueseptatum in Eucalyptus growing regions of India.
Cylindrocladium quinqueseptatum has been considered as the most destructive pathogen of Eucalyptu... more Cylindrocladium quinqueseptatum has been considered as the most destructive pathogen of Eucalyptus nurseries and plantations in north India. Genetic resistance has not been determined against this disease in Eucalyptus and genetic diversity among the fungal population in northern India is not known. Seventy three isolates from infected leaves and twigs of Eucalyptus were collected from different northern Indian state and analyzed through RAPD-PCR for screening genetic diversity. The UPGMA cluster analysis score of 284 loci permitted identification of 11 population lines and an outlier. This molecular variability prevalent among the north Indian population of the pathogen can used in identifying Cylindrocladium leaf and seedling blight resistant Eucalyptus germplasm.
Paper, pulp and plywood industries of north Indian states viz. Uttarakhand, Haryana and Punjab he... more Paper, pulp and plywood industries of north Indian states viz. Uttarakhand, Haryana and Punjab heavily depend on Eucalyptus plantations for raw material. However, Cylindrocladium leaf and seedling blight cause large scale mortality in nurseries thus jeopardizing plantation programs. In the present study 71 isolates of the fungal pathogen Cylindrocladium quinqueseptatum collected from three north Indian states were screened for their relative virulence. Representative pathogen isolates were screened on different culture media for variability in cultural and micro-morphological traits.
There is heavy biotic pressure on the Himalayan alpine meadows of Uttarakhand in India for the ex... more There is heavy biotic pressure on the Himalayan alpine meadows of Uttarakhand in India for the extraction of Cordyceps sinensis, a medicinally important fungus. Molecular characterization of Cordyceps sinensis germplasm of Indian origin has not yet been established. In the current study isolates of C. sinensis collected from different parts of Uttarakhand have been analyzed by Random Amplified Polymorphic DNA for assessing genetic diversity among the isolates.
Mohanty PS, Harsh NSK, Pandey A. 2011 -First report of Ganoderma resinaceum and G. weberianum fro... more Mohanty PS, Harsh NSK, Pandey A. 2011 -First report of Ganoderma resinaceum and G. weberianum from north India based on ITS sequence analysis and micromorphology. Mycosphere 2(4), 469-474.
We developed PCR primers to detect Cylindrocladium quinqueseptatum which infect Eucalyptus causin... more We developed PCR primers to detect Cylindrocladium quinqueseptatum which infect Eucalyptus causing leaf and seedling blight resulting in heavy seedling mortality in North Indian states. Primers based on sequence analysis of internal transcribed spacer region 1 and 5.8S of ribosomal DNA produced PCR product of 245bp. The internal transcribed spacer (ITS) of the ribosomal DNA (rDNA) sub unit repeat was sequenced in 26 isolates of Cylindrocladium quinqueseptatum and sequences were aligned and compared with the ITS sequences of other fungi in GenBank. No amplification resulted from PCR reactions on fungal DNA from 6 common forest fungi, 10 soil contaminates and 6 Eucalyptus pathogens. For amplifications directly from infected tissues, a nested primer PCR using two rounds of amplification was done. First, the entire ITS was amplified with universal fungal primer; a second round of amplification was carried out with species specific primer that amplified a 245 bp PCR product. The method detected leaf and seedling blight in artificially and naturally infected Eucalyptus plants. From the soil also the pathogen was detected using species specific primer. In sampling studies, C. quinqueseptatum was detected by PCR from artificially infected seedlings at 6 days post inoculation, before any visible symptoms were present. The PCR assay and direct tissue extraction methods provide tools which may be used to detect C. quinqueseptatum from soil, plant cuttings and adjoining Eucalyptus plantations serving as recurring source of infection and thus limit the transmission and spread of new aggressive strains of C. quinqueseptatum in Eucalyptus growing regions of India.
Cylindrocladium quinqueseptatum has been considered as the most destructive pathogen of Eucalyptu... more Cylindrocladium quinqueseptatum has been considered as the most destructive pathogen of Eucalyptus nurseries and plantations in north India. Genetic resistance has not been determined against this disease in Eucalyptus and genetic diversity among the fungal population in northern India is not known. Seventy three isolates from infected leaves and twigs of Eucalyptus were collected from different northern Indian state and analyzed through RAPD-PCR for screening genetic diversity. The UPGMA cluster analysis score of 284 loci permitted identification of 11 population lines and an outlier. This molecular variability prevalent among the north Indian population of the pathogen can used in identifying Cylindrocladium leaf and seedling blight resistant Eucalyptus germplasm.
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