Papers by Sriram Seshadri
Microbiome research reports, Oct 30, 2023
Gut microbiota research has gained a tremendous amount of attention from the scientific community... more Gut microbiota research has gained a tremendous amount of attention from the scientific community because of its contribution to gut homeostasis, human health, and various pathophysiological conditions. The early colonizer of the human gut, i.e., bifidobacteria, has emerged as an efficient probiotic in various diseased conditions, including cancer. This review explores the pros and cons of Bifidobacterium in various malignancies and various therapeutic strategies. We have illustrated the controversial role of bifidobacteria participating in various malignancies as well as described the current knowledge regarding its use in anticancer therapies. Ultimately, this article also addresses the need for further extensive research in elucidating the mechanism of how bifidobacteria is involved and is indirectly affecting the tumor microenvironment. Exhaustive and large-scale research is also required to solve the controversial questions regarding the involvement of bifidobacteria in cancer research.
Deleted Journal, May 24, 2024
The gut microbiota affects human health profoundly, and evidence is mounting that it can cause, w... more The gut microbiota affects human health profoundly, and evidence is mounting that it can cause, worsen, or resolve illnesses. Particularly in drug-induced toxicity, its role in diverse toxicological reactions has garnered attention recently. Drugs may interact directly or indirectly, through the gut flora, whether or not they are taken orally, changing the toxicity. Current research focuses mainly on the one-way effect of xenobiotics on the makeup and activities of gut microbes, which leads to altered homeostasis. However, there are two-way interactions between the gut microbiota and xenobiotics, and it is important to consider how the gut microbiota affects xenobiotics, particularly medications. Therefore, we emphasise the microbiome, microbial metabolites, and microbial enzymes in this review to emphasise how the gut microbiota affects medication toxicity. To aid in the identification of micro-biologic targets and processes linked to drug toxicity, we establish connections between medications, the microbiome, microbial enzymes or metabolites, drug metabolites, and host toxicological reactions. In addition, a summary and discussion of contemporary mainstream approaches to control medication toxicity by microbiota targeting are provided.
Journal of Drug Delivery Science and Technology, Jun 1, 2022
Journal of Molecular Structure, Nov 1, 2022
Chemischer Informationsdienst, Oct 2, 1984
Probiotics and Antimicrobial Proteins, Mar 17, 2013
The emergence of antibiotic resistance has increased the interest for finding new antimicrobials ... more The emergence of antibiotic resistance has increased the interest for finding new antimicrobials in the past decade. Probiotic Lactic acid bacteria producing antimicrobial proteins like bacteriocin can be excellent agents for development as novel therapeutic agents and complement to conventional antibiotic therapy. Uropathogenic Escherichia coli, most causative agent of Urinary tract infection, has developed resistance to various antibiotics. In the present investigation, antibacterial substance like bacteriocin (Bacteriocin DT24) produced by probiotic Lactobacillus brevis DT24 from vaginal sample of healthy Indian woman was partially purified and characterized. It was efficiently working against various pathogens, that is, Uropathogenic E. coli, Enterococcus faecium, Enterococcus faecalis and Staphylococcus aureus. The antimicrobial peptide was relatively heat resistant and also active over a broad range of pH 2-10. It has been partially purified by ammonium sulfate precipitation and gel filtration chromatography and checked on reverse-phase high-performance liquid chromatography. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of bacteriocin DT24 was approximately 7-kDa protein. The peptide is inactivated by proteolytic enzymes, trypsin and lipase but not when treated with catalase, α-amylase and pepsin. It showed bacteriostatic mode of action against uropathogenic E. coli. Such characteristics indicate that this bacteriocin-producing probiotic may be a potential candidate for alternative agents to control urinary tract infections and other pathogens.
Biotechnology and Bioprocess Engineering
Journal of Drug Delivery Science and Technology
Russian Journal of Organic Chemistry, 2021
A series of 2-amino-4-aryl-5-oxochromeno[4,3-b]pyridine-3-carbonitriles have been synthesized wit... more A series of 2-amino-4-aryl-5-oxochromeno[4,3-b]pyridine-3-carbonitriles have been synthesized with good yields (85–90%) by reacting 4-amino-2H-chromen-2-one, substituted benzaldehydes, and malononitrile at 80–85°C for 90–120 min using 1-butyl-3-methylimidazolium tetrafluoroborate as an ionic liquid medium. The synthesized derivatives have been screened for their anticancer potential on SKOV-3 and PC-3 cell lines, and the results were found to be encouraging. In addition, molecular descriptor prediction was performed for the compounds in terms of Lipinski’s rule of five; in particular, their topological polar surface areas (TPSA) and lipophilicities (iLogP) were found to be within an acceptable range.
Turkish Journal of Gastroenterology, 2020
Background: In the present study, we tried to understand the crosstalk between prostaglandins-COX... more Background: In the present study, we tried to understand the crosstalk between prostaglandins-COX-mediated rectal tumors and toll-like receptors in rats.Methods: The tumor was induced using nicotine (100 μL/mL). Following the induction, the serum and rectal tissue were analyzed for Lipo-polysaccharides (LPS) and prostaglandin E2 in serum, and tissue expression of inflammatory mediators like TLR2,4, NFkB; cancer markers like Matrix metalloproteases 2 (MMP2), 9 and Cyclo-oxygenases 2 (COX-2) were estimated. The gut microflora analysis was carried out using the fresh fecal samples of both the study groups.Results: In nicotine-induced group, there was a significant alteration in the gut microflora toward high Gram-negative strains and a decline in Gram-positive populations. All the inflammatory as well as cancer prognostic markers were significantly increased in the tumor-induced animals.Conclusion: From the present study, it could be concluded that nicotine significantly induced rectal cancer in the mice model by modulating gut microflora and increasing COX-2 and prostaglandin E2 levels.
Experimental Oncology, 2019
Summary. Asafoetida resin has been reported for various biological activities but its use has bee... more Summary. Asafoetida resin has been reported for various biological activities but its use has been widely restricted owing to its pungent smell and pool water solubility. Aim: In vitro study of the anticancer potential of microwave-extracted essential oil (EO) of Ferula asafoetida. Materials and Methods: The phytochemical investigation and in vitro cytotoxicity assessment was carried out in two human liver cancer cell lines. The expression of NFKB1, TGFB1, TNF, CASP3 was analyzed by reverse transcription polymerase chain reaction. Results: Ferula asafoetida EO contains high concentrations of dithiolane, which possess antiproliferative activity in human liver carcinoma cell lines (HepG2 and SK-Hep1) in a dose-dependent manner. The bioactive compounds in F. asafoetida are capable of induction of apoptosis and altered NF-kB and TGF-β signalling with increase in caspase-3 and TNF-α expression. Conclusion: Further elucidation of bioactive molecules and underlying mechanisms could lead to...
Immunome Research, 2016
Type 2 diabetes is characterized by peripheral insulin resistance. Besides immune and inflammator... more Type 2 diabetes is characterized by peripheral insulin resistance. Besides immune and inflammatory mechanisms, other pathways involve interaction between gut microbiota and metabolic syndrome. The present study was designed to understand gut microbiota alteration following High Sugar Diet (HSD) and its effect on physiology and gastrointestinal immunology. Male wistar rats were fed with high fructose and HSD for 60 days. Composition of fecal microbiota by DGGE and proinflammatory cytokines in serum was investigated. Expressions of genes such as TLR2, TLR4 and NF-kB in various tissues were also studied. The bacteria coliforms and clostridium level were higher and Lactobacillus was lower in both sugar rich diet fed rats. Highly diverse and densely populated bands were observed in HSD group by DGGE fingerprint. The band profiles of sugar fed group have clustered together. Elevated mRNA expression of TLR2, TLR4, and NF-kB were observed in HSD groups. Increased inflammation was confirmed by blood and tissue biochemical assay and enhanced serum pro-inflammatory cytokines in HSD diet groups. Gut microbiota strongly influenced the metabolic profiling of individuals fed with high calorie intake. The diverse microbial population and increased coliforms and clostridium may affect host gene expression. Targeting TLRs and microbiota could be promising therapeutic approach.
Progress in Molecular Biology and Translational Science
The human microbiome is increasingly being showed to have a direct and intimate relationship to h... more The human microbiome is increasingly being showed to have a direct and intimate relationship to human health and various disease conditions. Most of this microbiome is resident in the human gut, of which the colon is an important part. Microbial members of this gut community actively communicate among themselves through the process of quorum sensing (QS), and also with their human host. The level and nature of these communication processes play a crucial role in determining the overall health status, and susceptibility to various diseases, of the human system. Controlled modulation of QS-based and other communication processes within the human gut can prove to be a very effective tool in safeguarding human health. However, to achieve the full potential of any such approach, targeted delivery of QS-modulators specifically to the parts of human body, like the colon, which are characterized by high microbial density, is desired. Nanotechnological approaches can be of considerable use i...
Indian Journal of Microbiology, 2022
Alteration of gut microflora results in a metabolic imbalance in the liver. In the present study,... more Alteration of gut microflora results in a metabolic imbalance in the liver. In the present study, we investigate the reversal potential of alteration of the colonic microflora via improving metabolism balance and regulating the altered tight junction of the intestinal tract. Animals were fed with high sugar diet to mimic the onset of the pathophysiological conditions of diabetes. Following induction, animals were divided into two reversal groups i.e., crude cefdinir and colon-specific formulated cefdinir, to alter the gut microflora. In the present study, we have tried to quantify the microbial content via metagenome analysis to provide an actual picture of the alteration and subsequent reversal. Expression of mRNA of junctional protein and parameters involved in liver metabolism was determined using qPCR. Results indicated direct effect of altered composition of gut microflora on the gut permeability and metabolic alteration. Metagenomic analysis showed least evenness and richness in the HSD group whereas antibiotic-treated groups showed reversal of microflora towards control group with increased richness, evenness and decreased distance on PCoA plot. This changes in gut microflora composition changes expression of metabolic markers and thus insulin sensitivity. Targeting colonic microflora to have a reversal effect on T2D pathogenesis, found to have a positive impact on liver metabolic state with improved permeability markers of gut with SCFA alteration.
Copyright © 2014 Disha Trivedi et al. This is an open access article distributed under the Creati... more Copyright © 2014 Disha Trivedi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Novel therapeutic approaches are needed to combat the urinary tract infection in women. During menstruation elevated protein concentration and increase in oxygen and carbon dioxide concentrations with decrease in vaginal Lactobacilli all together contribute to urinary tract infections. Lactobacillus species are a predominant member of the vaginal microflora and are critical in the prevention of a number of urogenital diseases. In order to increase antimicrobial potential of vaginal Lactobacilli, bacteriocin colicin E2 which has specific activity against uropathogenic Escherichia coli has been overexpressed in vaginal probiotic Lactobacillus brevis DT24. Recombinant Lactobacillus brevis DT24 expressing colicin E2 showed much higher inh...
Multidrug resistance in Pseudomonas aeruginosa: Insights into the molecular mechanisms
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Papers by Sriram Seshadri