Nanoparticle Fabrication

Development and production of lipid nanoparticles (LNPs) and other nanocarriers using advanced microfluidic systems.

Instrumentation

• Nova™IJM – Advanced lipid nanoparticle (LNP) manufacturing platform
• UnChained Labs Sunshine – Enabling formulation development from in vitro to in vivo studies

Exosomes Isolation and Characterization

Isolation and analysis of extracellular vesicles from biological samples.

Isolation Methods

• Exosome isolation from cell culture media and tissues using ExoQuick® ULTRA EV Isolation System, ultracentrifugation, and size exclusion chromatography

Characterization

• Exosomal markers analysis (Western blot, Exo-Check™ Exosome Antibody Array)
• Proteomics analysis
• Particle size, concentration, purity assessment, and more 

Analytical Characterization

Comprehensive analytical methods for nanoparticle, polymer, and biomolecule characterization.

Particle Characterization

• Particle size, size distribution, concentration, and surface charge
• Dynamic Light Scattering (DLS)
• Zetaview (ParticleMetrix)
• Multisizer 4E Coulter Counter

Materials Analysis

• Gel Permeation Chromatography (GPC): weight-average molar mass (Mw), number-average molar mass (Mn), and polydispersity
• Structural analysis using Infrared spectroscopy (IR) and Thermogravimetric analysis (TGA)

Chemical Analysis

• High Performance Liquid Chromotography (HPLC)
• UV-Vis spectroscopy
• Temperature-dependent UV analysis

Protein, Peptide, and RNA Analysis

• Capillary electrophoresis (protein purity, isoelectric focusing, Glycan analysis)
• Automated Western blot analysis (Protein Simple)
• Gel imaging

Elemental Analysis

• Metals analysis in soils, water, food, plants, cells, and tissues using ICP-MS.

In Vitro Assays

• Protein concentration (BCA assay).
• Endotoxin contamination (LAL assay).
• In vitro stability analysis.
• In vitro drug release analysis.
• In vitro cellular uptake, transfection and toxicity analysis.

Consultation Services

We offer expert consultation on:

• Study design
• Sample selection, collection, and storage
• Data analysis
• Proposal and manuscript development and review, and more