Genetic diversity assessment with different methods and their comparison could provide complement... more Genetic diversity assessment with different methods and their comparison could provide complementary information for improvement and conservation programs. However, such information is not available for Ethiopian lentil landraces. To fill this gap, nine morphological traits and four ISSR primers were used on seventy Ethiopian lentil landrace accessions to study morphological and genetic diversity among the accessions, assess the correlation between phenotypic and genetic dissimilarities, and classify the accessions into groups based on both markers. Analysis of variance of morphological data showed significant (p < 0.01) differences among accessions for all traits. However, days to maturity and flowering, seed weight and plant height showed low phenotypic coefficient of variation. From principal component (PC) analysis number of seeds and pods, seed yield and number of secondary branch were included in PC1, whereas, days to maturity and flowering were dominant in PC2. Seed weight...
Phenotypic and genetic diversity of ten improved lentil varieties of Ethiopia were assessed using... more Phenotypic and genetic diversity of ten improved lentil varieties of Ethiopia were assessed using nine agro-morphological characters and four ISSR primers. ANOVA of phenotypic data showed highly significant variation among the varieties for all traits. Number of pods and seeds, hundred seed weight, and number of secondary and primary branch showed high phenotypic coefficient of variation (PCV), while days to flowering, seed yield, plant height and days to maturity revealed low PCV. Ada'a, Chalew and R-186 were late in flowering and maturity, while Gudo was early flowering, but late maturing variety.
Phenotypic and genetic diversity of ten improved lentil varieties of Ethiopia were assessed using... more Phenotypic and genetic diversity of ten improved lentil varieties of Ethiopia were assessed using nine agro-morphological characters and four ISSR primers. ANOVA of phenotypic data showed highly significant variation among the varieties for all traits. Number of pods and seeds, hundred seed weight, and number of secondary and primary branch showed high phenotypic coefficient of variation (PCV), while days to flowering, seed yield, plant height and days to maturity revealed low PCV. Ada’a, Chalew and R-186 were late in flowering and maturity, while Gudo was early flowering, but late maturing variety. Alemaya, Alemtena and Assano were early in flowering and maturity, while Chekol, EL-142 and Teshale were intermediate in flowering but early in maturity. The Euclidian distance between Ada’a and Assano was high, while the distance between Alemtena and Teshale was the least. Clustering analysis based on phenotypic data classified the varieties into two groups. ISSR analyses revealed that ...
Characterization of genetic resources maintained at genebanks has important implication for futur... more Characterization of genetic resources maintained at genebanks has important implication for future utilization and collection activities. The objective of this study was to assess the level of genetic diversity, genetic structure and genetic distance, and to indirectly estimate the level of gene flow among populations of Ethiopian lentil landraces using intersimple sequence repeat (ISSR) marker. For this purpose, seeds of seventy landrace accessions collected from seven different administrative regions of Ethiopia were obtained from Inistitute of Biodiversity Conservation and grown at Sinana Agricultural Research Center (Bale-Robe, Ethiopia) on-station field plot. DNA was extracted from a bulk leaf sample of fifteen randomly selected seed-raised plants using a triple CTAB extraction technique. ISSR data were generated using four primers. The total genetic diversity (T H), the intrapopulation genetic diversity (S H) and the interpopulation genetic diversity (ST D) were 0.175, 0.095 and 0.079, respectively. High intrapopulation genetic diversity was observed for Gonder, Shewa and Wello populations, while Arsi, Bale, Tigray and Gojam populations showed low intrapopulation diversity. There was high genetic differentiation (G ST = 0.455) but intermediate gene flow level (Nm = 0.60) among populations. The genetic distance (D) between populations ranged from 0.012-0.228. Cluster analysis revealed two groups of Ethiopian lentil landrace populations. The results provide important baseline for future germplasm conservation and improvement programs.
All rights reserved. No part of this periodical may be reproduced or transmitted in any form or b... more All rights reserved. No part of this periodical may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Permission for printing and for reprinting the material contained herein has been obtained by the publisher.
International Journal of Genetics and Molecular Biology, May 30, 2011
Phenotypic and genetic diversity of ten improved lentil varieties of Ethiopia were assessed using... more Phenotypic and genetic diversity of ten improved lentil varieties of Ethiopia were assessed using nine agro-morphological characters and four ISSR primers. ANOVA of phenotypic data showed highly significant variation among the varieties for all traits. Number of pods and seeds, hundred seed weight, and number of secondary and primary branch showed high phenotypic coefficient of variation (PCV), while days to flowering, seed yield, plant height and days to maturity revealed low PCV. Ada'a, Chalew and R-186 were late in flowering and maturity, while Gudo was early flowering, but late maturing variety. Alemaya, Alemtena and Assano were early in flowering and maturity, while Chekol, EL-142 and Teshale were intermediate in flowering but early in maturity. The Euclidian distance between Ada'a and Assano was high, while the distance between Alemtena and Teshale was the least. Clustering analysis based on phenotypic data classified the varieties into two groups. ISSR analyses revealed that 75.93% of the amplified bands were polymorphic with average gene diversity ( H ) of 0.2734. Inter-varietal genetic distance between Gudo and Chekol was high, while that between Gudo and Alemaya was low. Clustering analysis based on ISSR marker also classified the varieties into two groups. The correlation ( r =0.334) between morphological and ISSR dissimilarity matrices was significant. Results of the present study reveal the genetic variation between Ethiopian lentil varieties which would help in selection of varieties for specific agro-ecology and/or purpose, and for further breeding activities.
Mulusew Fikere, Tadele Tadesse and Tesfaye Letta .2008. Genotype-Environment Interactions and Sta... more Mulusew Fikere, Tadele Tadesse and Tesfaye Letta .2008. Genotype-Environment Interactions and Stability Parameters for Grain Yield of Faba Bean (Vacia faba L.) Genotypes Grown in South Eastern Ethiopia. Int. J. Sustain. Crop Prod. 3(6):80-87 Grain yield of 16 faba bean (Vacia faba L.) genotypes tested in a Randomized Complete Block Design (RCBD) with four replications across 12 environments during 2004-2006 growing season of South Eastern
Phenotypic and genetic diversity of ten improved lentil varieties of Ethiopia were assessed using... more Phenotypic and genetic diversity of ten improved lentil varieties of Ethiopia were assessed using nine agro-morphological characters and four ISSR primers. ANOVA of phenotypic data showed highly significant variation among the varieties for all traits. Number of pods and seeds, hundred seed weight, and number of secondary and primary branch showed high phenotypic coefficient of variation (PCV), while days to flowering, seed yield, plant height and days to maturity revealed low PCV. Ada'a, Chalew and R-186 were late in flowering and maturity, while Gudo was early flowering, but late maturing variety. Alemaya, Alemtena and Assano were early in flowering and maturity, while Chekol, EL-142 and Teshale were intermediate in flowering but early in maturity. The Euclidian distance between Ada'a and Assano was high, while the distance between Alemtena and Teshale was the least. Clustering analysis based on phenotypic data classified the varieties into two groups. ISSR analyses revealed that 75.93% of the amplified bands were polymorphic with average gene diversity ( H ) of 0.2734. Inter-varietal genetic distance between Gudo and Chekol was high, while that between Gudo and Alemaya was low. Clustering analysis based on ISSR marker also classified the varieties into two groups. The correlation ( r =0.334) between morphological and ISSR dissimilarity matrices was significant. Results of the present study reveal the genetic variation between Ethiopian lentil varieties which would help in selection of varieties for specific agro-ecology and/or purpose, and for further breeding activities.
One hundred and sixty-four accessions representing Czech and Slovak pea (Pisum sativum L.) variet... more One hundred and sixty-four accessions representing Czech and Slovak pea (Pisum sativum L.) varieties bred over the last 50 years were evaluated for genetic diversity using morphological, simple sequence repeat (SSR) and retrotransposon-based insertion polymorphism (RBIP) markers. Polymorphic information content (PIC) values of 10 SSR loci and 31 RBIP markers were on average high at 0.89 and 0.73, respectively. The silhouette method after the Ward clustering produced the most probable cluster estimate, identifying nine clusters from molecular data and Wve to seven clusters from morphological characters. Principal component analysis of nine qualitative and eight quantitative morphological parameters explain over 90 and 93% of total variability, respectively, in the Wrst three axes. Multidimensional scaling of molecular data revealed a continuous structure for the set. To enable integration and evaluation of all data types, a Bayesian method for clustering was applied. Three clusters identiWed using morphology data, with clear separation of fodder, dry seed and aWla types, were resolved by DNA data into 17, 12 and Wve subclusters, respectively. A core collection of 34 samples was derived from the complete collection by BAPS Bayesian analysis. Values for average gene diversity and allelic richness for molecular marker loci and diversity indexes of phenotypic data were found to be similar between the two collections, showing that this is a useful approach for representative core selection.
Genetic diversity assessment with different methods and their comparison could provide complement... more Genetic diversity assessment with different methods and their comparison could provide complementary information for improvement and conservation programs. However, such information is not available for Ethiopian lentil landraces. To fill this gap, nine morphological traits and four ISSR primers were used on seventy Ethiopian lentil landrace accessions to study morphological and genetic diversity among the accessions, assess the correlation between phenotypic and genetic dissimilarities, and classify the accessions into groups based on both markers. Analysis of variance of morphological data showed significant (p < 0.01) differences among accessions for all traits. However, days to maturity and flowering, seed weight and plant height showed low phenotypic coefficient of variation. From principal component (PC) analysis number of seeds and pods, seed yield and number of secondary branch were included in PC1, whereas, days to maturity and flowering were dominant in PC2. Seed weight and number of primary branch were also dominant in PC3 and PC4, respectively. With regard to molecular analysis, accessions from Gonder, Shewa and Wello (with I = 0.203, 0.174 and 0.162, respectively) showed moderate level of diversity. The average and overall diversity index values, respectively, were 0.145 and 0.264. The overall morphological and genetic dissimilarities ranged from 0.825 -11.018 and 0.000 -0.340 with average value of 3.921 and 0.125, respectively. The correlation ( r )
Genetic diversity assessment with different methods and their comparison could provide complement... more Genetic diversity assessment with different methods and their comparison could provide complementary information for improvement and conservation programs. However, such information is not available for Ethiopian lentil landraces. To fill this gap, nine morphological traits and four ISSR primers were used on seventy Ethiopian lentil landrace accessions to study morphological and genetic diversity among the accessions, assess the correlation between phenotypic and genetic dissimilarities, and classify the accessions into groups based on both markers. Analysis of variance of morphological data showed significant (p < 0.01) differences among accessions for all traits. However, days to maturity and flowering, seed weight and plant height showed low phenotypic coefficient of variation. From principal component (PC) analysis number of seeds and pods, seed yield and number of secondary branch were included in PC1, whereas, days to maturity and flowering were dominant in PC2. Seed weight...
Phenotypic and genetic diversity of ten improved lentil varieties of Ethiopia were assessed using... more Phenotypic and genetic diversity of ten improved lentil varieties of Ethiopia were assessed using nine agro-morphological characters and four ISSR primers. ANOVA of phenotypic data showed highly significant variation among the varieties for all traits. Number of pods and seeds, hundred seed weight, and number of secondary and primary branch showed high phenotypic coefficient of variation (PCV), while days to flowering, seed yield, plant height and days to maturity revealed low PCV. Ada'a, Chalew and R-186 were late in flowering and maturity, while Gudo was early flowering, but late maturing variety.
Phenotypic and genetic diversity of ten improved lentil varieties of Ethiopia were assessed using... more Phenotypic and genetic diversity of ten improved lentil varieties of Ethiopia were assessed using nine agro-morphological characters and four ISSR primers. ANOVA of phenotypic data showed highly significant variation among the varieties for all traits. Number of pods and seeds, hundred seed weight, and number of secondary and primary branch showed high phenotypic coefficient of variation (PCV), while days to flowering, seed yield, plant height and days to maturity revealed low PCV. Ada’a, Chalew and R-186 were late in flowering and maturity, while Gudo was early flowering, but late maturing variety. Alemaya, Alemtena and Assano were early in flowering and maturity, while Chekol, EL-142 and Teshale were intermediate in flowering but early in maturity. The Euclidian distance between Ada’a and Assano was high, while the distance between Alemtena and Teshale was the least. Clustering analysis based on phenotypic data classified the varieties into two groups. ISSR analyses revealed that ...
Characterization of genetic resources maintained at genebanks has important implication for futur... more Characterization of genetic resources maintained at genebanks has important implication for future utilization and collection activities. The objective of this study was to assess the level of genetic diversity, genetic structure and genetic distance, and to indirectly estimate the level of gene flow among populations of Ethiopian lentil landraces using intersimple sequence repeat (ISSR) marker. For this purpose, seeds of seventy landrace accessions collected from seven different administrative regions of Ethiopia were obtained from Inistitute of Biodiversity Conservation and grown at Sinana Agricultural Research Center (Bale-Robe, Ethiopia) on-station field plot. DNA was extracted from a bulk leaf sample of fifteen randomly selected seed-raised plants using a triple CTAB extraction technique. ISSR data were generated using four primers. The total genetic diversity (T H), the intrapopulation genetic diversity (S H) and the interpopulation genetic diversity (ST D) were 0.175, 0.095 and 0.079, respectively. High intrapopulation genetic diversity was observed for Gonder, Shewa and Wello populations, while Arsi, Bale, Tigray and Gojam populations showed low intrapopulation diversity. There was high genetic differentiation (G ST = 0.455) but intermediate gene flow level (Nm = 0.60) among populations. The genetic distance (D) between populations ranged from 0.012-0.228. Cluster analysis revealed two groups of Ethiopian lentil landrace populations. The results provide important baseline for future germplasm conservation and improvement programs.
All rights reserved. No part of this periodical may be reproduced or transmitted in any form or b... more All rights reserved. No part of this periodical may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or any information storage and retrieval system, without permission in writing from the publisher. Permission for printing and for reprinting the material contained herein has been obtained by the publisher.
International Journal of Genetics and Molecular Biology, May 30, 2011
Phenotypic and genetic diversity of ten improved lentil varieties of Ethiopia were assessed using... more Phenotypic and genetic diversity of ten improved lentil varieties of Ethiopia were assessed using nine agro-morphological characters and four ISSR primers. ANOVA of phenotypic data showed highly significant variation among the varieties for all traits. Number of pods and seeds, hundred seed weight, and number of secondary and primary branch showed high phenotypic coefficient of variation (PCV), while days to flowering, seed yield, plant height and days to maturity revealed low PCV. Ada'a, Chalew and R-186 were late in flowering and maturity, while Gudo was early flowering, but late maturing variety. Alemaya, Alemtena and Assano were early in flowering and maturity, while Chekol, EL-142 and Teshale were intermediate in flowering but early in maturity. The Euclidian distance between Ada'a and Assano was high, while the distance between Alemtena and Teshale was the least. Clustering analysis based on phenotypic data classified the varieties into two groups. ISSR analyses revealed that 75.93% of the amplified bands were polymorphic with average gene diversity ( H ) of 0.2734. Inter-varietal genetic distance between Gudo and Chekol was high, while that between Gudo and Alemaya was low. Clustering analysis based on ISSR marker also classified the varieties into two groups. The correlation ( r =0.334) between morphological and ISSR dissimilarity matrices was significant. Results of the present study reveal the genetic variation between Ethiopian lentil varieties which would help in selection of varieties for specific agro-ecology and/or purpose, and for further breeding activities.
Mulusew Fikere, Tadele Tadesse and Tesfaye Letta .2008. Genotype-Environment Interactions and Sta... more Mulusew Fikere, Tadele Tadesse and Tesfaye Letta .2008. Genotype-Environment Interactions and Stability Parameters for Grain Yield of Faba Bean (Vacia faba L.) Genotypes Grown in South Eastern Ethiopia. Int. J. Sustain. Crop Prod. 3(6):80-87 Grain yield of 16 faba bean (Vacia faba L.) genotypes tested in a Randomized Complete Block Design (RCBD) with four replications across 12 environments during 2004-2006 growing season of South Eastern
Phenotypic and genetic diversity of ten improved lentil varieties of Ethiopia were assessed using... more Phenotypic and genetic diversity of ten improved lentil varieties of Ethiopia were assessed using nine agro-morphological characters and four ISSR primers. ANOVA of phenotypic data showed highly significant variation among the varieties for all traits. Number of pods and seeds, hundred seed weight, and number of secondary and primary branch showed high phenotypic coefficient of variation (PCV), while days to flowering, seed yield, plant height and days to maturity revealed low PCV. Ada'a, Chalew and R-186 were late in flowering and maturity, while Gudo was early flowering, but late maturing variety. Alemaya, Alemtena and Assano were early in flowering and maturity, while Chekol, EL-142 and Teshale were intermediate in flowering but early in maturity. The Euclidian distance between Ada'a and Assano was high, while the distance between Alemtena and Teshale was the least. Clustering analysis based on phenotypic data classified the varieties into two groups. ISSR analyses revealed that 75.93% of the amplified bands were polymorphic with average gene diversity ( H ) of 0.2734. Inter-varietal genetic distance between Gudo and Chekol was high, while that between Gudo and Alemaya was low. Clustering analysis based on ISSR marker also classified the varieties into two groups. The correlation ( r =0.334) between morphological and ISSR dissimilarity matrices was significant. Results of the present study reveal the genetic variation between Ethiopian lentil varieties which would help in selection of varieties for specific agro-ecology and/or purpose, and for further breeding activities.
One hundred and sixty-four accessions representing Czech and Slovak pea (Pisum sativum L.) variet... more One hundred and sixty-four accessions representing Czech and Slovak pea (Pisum sativum L.) varieties bred over the last 50 years were evaluated for genetic diversity using morphological, simple sequence repeat (SSR) and retrotransposon-based insertion polymorphism (RBIP) markers. Polymorphic information content (PIC) values of 10 SSR loci and 31 RBIP markers were on average high at 0.89 and 0.73, respectively. The silhouette method after the Ward clustering produced the most probable cluster estimate, identifying nine clusters from molecular data and Wve to seven clusters from morphological characters. Principal component analysis of nine qualitative and eight quantitative morphological parameters explain over 90 and 93% of total variability, respectively, in the Wrst three axes. Multidimensional scaling of molecular data revealed a continuous structure for the set. To enable integration and evaluation of all data types, a Bayesian method for clustering was applied. Three clusters identiWed using morphology data, with clear separation of fodder, dry seed and aWla types, were resolved by DNA data into 17, 12 and Wve subclusters, respectively. A core collection of 34 samples was derived from the complete collection by BAPS Bayesian analysis. Values for average gene diversity and allelic richness for molecular marker loci and diversity indexes of phenotypic data were found to be similar between the two collections, showing that this is a useful approach for representative core selection.
Genetic diversity assessment with different methods and their comparison could provide complement... more Genetic diversity assessment with different methods and their comparison could provide complementary information for improvement and conservation programs. However, such information is not available for Ethiopian lentil landraces. To fill this gap, nine morphological traits and four ISSR primers were used on seventy Ethiopian lentil landrace accessions to study morphological and genetic diversity among the accessions, assess the correlation between phenotypic and genetic dissimilarities, and classify the accessions into groups based on both markers. Analysis of variance of morphological data showed significant (p < 0.01) differences among accessions for all traits. However, days to maturity and flowering, seed weight and plant height showed low phenotypic coefficient of variation. From principal component (PC) analysis number of seeds and pods, seed yield and number of secondary branch were included in PC1, whereas, days to maturity and flowering were dominant in PC2. Seed weight and number of primary branch were also dominant in PC3 and PC4, respectively. With regard to molecular analysis, accessions from Gonder, Shewa and Wello (with I = 0.203, 0.174 and 0.162, respectively) showed moderate level of diversity. The average and overall diversity index values, respectively, were 0.145 and 0.264. The overall morphological and genetic dissimilarities ranged from 0.825 -11.018 and 0.000 -0.340 with average value of 3.921 and 0.125, respectively. The correlation ( r )
Uploads
Papers by Edossa Fikiru