Papers by armin imanparast
Iranian Journal of Medical Physics, Sep 1, 2021
Photochemical internalization is a novel PDT-based technology for the intracellular delivery of h... more Photochemical internalization is a novel PDT-based technology for the intracellular delivery of hydrophilic macromolecular therapeutic agents and other drugs limited in penetration into cellular membranes with intracellular targets. In this regard, one of the approaches is to use nanoparticles along with photosensitizing agents. In this study, the presence of thioglucose-coated gold nanoparticles in the efficiency of the photodynamic effect of methylene blue (MB) caused by the photochemical internalization phenomenon was investigated. Material and Methods: First, Glu-GNPs was synthesized, and then the toxicity of Glu-GNPs and MB were determined to achieve their optimal concentrations. Afterward, the photodynamic effects of Glu-GNPs combined with MB by Luma-Care source light were evaluated at different doses using MTT assay and colony assay (12 days after treatment). Results: According to the MTT assay, the photodynamic effect in the Glu-GNPs group revealed no significant efficacy, whereas the colony-formation capability in all groups with an optical dose of 15.6 J / cm 2 decreased, compared to the similar group without light exposure (P 0.05). Conclusion: The photodynamic efficiency of MB with the Glu-GNPs group was reduced at 15.6 J/cm 2 , compared to the free MB group. The decreased efficiency can have various reasons such as the photochemical bleaching of the free MB because of ROS and 1 O2 produced by the plasmonic photodynamic phenomenon of Glu-GNPs or changing the optophysical properties of surface plasmon resonance of final product (MB+ Glu-GNPs) due to the possible electrostatic bonding of the drug with the nanoparticles.
Photodiagnosis and photodynamic therapy, Jan 14, 2018
In this study, iron oxide nanoparticles (SPIONs) were synthesized and coated by GA (SG) and then ... more In this study, iron oxide nanoparticles (SPIONs) were synthesized and coated by GA (SG) and then SG was encapsulated by ICG (SGI). After identifying specifications and cytotoxicity of the agents, the potential of SGI for photodynamic therapy (PDT) and photothermal therapy (PTT) was studied. An SGI size of 12-13 nm was determined by TEM images and its zeta potential was measured at -23.8 ± 5.8 mV. MCF-7 and HT-29 cells were exposed to a non-coherent light source at a wavelength of 730 nm and a range of 3.9-124.8 J/cm under two different concentrations of agents. The viability of treated cells was determined via MTT assay. To analyze the effects of different irradiation conditions, some indices such as Coefficient of Light Effect, Synergism Index, Addition Ratio, Treatment Efficacy and ED were defined. Cell survival at the highest power of irradiation in the absence of any agent was decreased to 93% and 73% for HT-29 and MCF-7, respectively. In both cell lines, the cellular survival d...
The Cancer Press, 2016
Colorectal cancer (CRC) is the third major cause of cancer death globally, with about 694,000 dea... more Colorectal cancer (CRC) is the third major cause of cancer death globally, with about 694,000 deaths each year. Recent studies have shown that cancer stem cells (CSCs) play an important role in cancer relapse and metastasis. Inhibition of COX-2, Wnt / beta-catenin, HIF-1 alpha, K-Ras, NF-kβ, P53 signaling pathways and activation of TGF-β are amongst proposed strategies against colorectal cancer. Specific markers of CSCs including CD133, CD24, CD29, CD44 and Lgr5 are also of importance in diagnosis and monitoring of the disease. Therefore, the use of nanoparticles can be discussed in two different fields: 1. using nanoparticles in diagnosis and targeting CSCs, 2. targeting the cancer cells directly, where nanoparticles can affect the cell signaling pathways and expression patterns to prevent extension of tumor cells. In addition, nanoparticles can be used as the carriers for anti-cancer drugs and drug delivery, which in turn may enhance effectiveness of treatment. This review covers the novel signaling pathways in colorectal cancer and also gives a brief explanation about the role of some nanostructures that enhance treatment efficiency.
Journal of Cellular Immunotherapy, 2015
Introduction: Approximately more than 930,000 cases were diagnosed with gastric cancer throughout... more Introduction: Approximately more than 930,000 cases were diagnosed with gastric cancer throughout the world. Overall Survival (OS) in patients with gastric cancer is less than 14 months. In Iran, North and North West regions are at high risk of gastric cancer. Significant differences in the frequency of environmental risk factors give the country an opportunity to investigate the causes of stomach cance. CD44 is the main molecule for cellular connections and it plays an important role in a number of physiological processes including cell attachment, migration and growth regulation. In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11. Moreover, CD44 increases proliferating tumor cells in cooperation with membrane-bound receptor tyrosine kinases containing C-src and C-erbB-2 (HER2/neu). Another important function of CD44 is to regulate tumor cell binding and motility. CD44 is involved in cell growth and metastasis formation of gastric and colorectal cancer. According to the study in California of single nucleotide polymorphisms rs187116 and rs7116432 with TTR and OS, we investigated the presence of these polymorphisms among patients with gastric cancer in Iran. We also investigated whether there is a strong significant association between these polymorphisms and clinical and pathological features of patients. Material & method: 150 patients with gastric cancer and 150 healthy subjects participated in the study. All patients in Imam Reza and Omid Hospitals of Mashhad University of Medical Sciences, from 1998 to 2010, were treated through surgery only or surgery with (radio) chemotherapy. The study was approved by the Ethical Committee of Mashhad University of Medical Sciences (MUMS) and consent forms from all patients were obtained before any procedures. Patients' formalin-fixed paraffin-embedded (FFPE) tissues and healthy people's blood samples were taken. Then genomic DNA was extracted using an extraction kit (QIAamp) according to the manufacturer's protocol. Samples were tested by restriction fragment length polymorphism (PCR-RFLP) analysis or direct sequencing. Genes, SNP reference identification numbers, location SNP, SNP performance, forward primer, reverse primer and restriction enzymes are summarized in Table 1. Joint and functional polymorphisms were selected in the CD44 gene according to previous articles. Our criteria for selecting a candidate gene polymorphism are as follows: low frequency of alleles 10% (MAF) in Caucasians and significant relationship with tumor reccurence and overall survival (Table 1). The primary endpoints of the analysis of polymorphisms in CD44 gene, included the time to tumor recurrence (TTR) and overall survival (OS), calculated from the date of diagnosis to date of the first observation of tumor recurrence or last follow-up, and the last case was used when the patient did not undergo tumor recurrence at that time. The TTR was calculated from the date of diagnosis of the disease to the date of first observation of tumor recurrence or until last follow-up if the patient was recurrence-free at that time. The OS wad defined as the time between disease diagnosis and death time to any cause or if the
Photodiagnosis and photodynamic therapy, Jan 23, 2018
In recent years, Mitoxantrone (MTX) has been introduced as a chemotherapy drug which also serves ... more In recent years, Mitoxantrone (MTX) has been introduced as a chemotherapy drug which also serves as a photosensitizer and radiosensitizer. Due to its serious side effects, there are limitations to the application of MTX so scientists are looking for solutions to overcome this problem. Hollow gold nanoparticles (HAuNP) have attracted growing attention due to their unique physical-chemical properties, such as biocompatibility, tunable plasmonic absorption peak ranging from visible to near infrared, high stability and various medical applications in imaging, drug delivery and combinational cancer treatments. In this paper, the combinational effect of photodynamic therapy (PDT) and chemotherapy of MTX conjugated to HAuNP is studied. After optimizing the synthesis of PEGylated HAuNP and preparing nanostructures conjugated with MTX, the characteristics of pharmacological agents including MTX, HAuNP, mPEG-HAuNP,and MTX-mPEG-HAuNP and their toxicity were determined at different concentratio...
Nanoparticles have been at the center of research focus as a new promising material for the treat... more Nanoparticles have been at the center of research focus as a new promising material for the treatment of cancer in recent years. Although many chemotherapy drugs for cancer treatment are available, their potential toxicity is the main point of concern. On the other hand, the conventional chemotherapeutic approach has not been found to be very efficient in colorectal cancer (CRC) as the drug molecule does not reach the target site with an effective concentration. A major challenge in cancer therapy is to destroy tumor cells without harming the normal tissue. To overcome this problem scientists are trying to use nanoparticles to directly target cancer cells for a more effective treatment and reduced toxicity. Different nanoparticles such as: liposomes, polymeric nanoparticles, dendrimers, and silica have been developed to carry a variety of anticancer agents including: cytotoxic drugs, chemo modulators, siRNA and antiangiogenic agents. This review discusses various treatments for colo...
Iranian Journal of Medical Physics, 2018
Introduction: The most important limitation of 5-aminolevulinic acid (5-ALA)-induced photodynamic... more Introduction: The most important limitation of 5-aminolevulinic acid (5-ALA)-induced photodynamic therapy (PDT) is the efficacy of the cells in converting 5-ALA to protoporphyrin IX. The present study aimed to investigate the effectiveness of silver nanoparticles (AgNPs) with the photosensitivity at the surface plasmon resonance wavelength on 5-ALA-mediated PDT. Material and Methods: First of all, the toxicity of 5-ALA, AgNPs, and combined 5-ALA and AgNPs was evaluated on DFW cell line derived from melanoma. After choosing the optimal concentration, both pulsed and continuous light irradiations were conducted at different doses using a light‑emitting diode source in the groups receiving 5-ALA, AgNPs, as well as 5-ALA and AgNPs combination, and the controls. The cell survival was evaluated 24 h after irradiation using MTT assay. Results: According to the results, light exposure did not significantly change cell survival in the absence or presence of AgNPs. However, light exposure in ...
Iranian Journal of Medical Physics, 2019
Introduction: Mitoxantrone (MX) has been introduced as a photosensitizer drug. However, due to so... more Introduction: Mitoxantrone (MX) has been introduced as a photosensitizer drug. However, due to some side effects, the widespread use of this drug has been confronted with some limitations. Hollow gold nanoshells (HGN) have attracted considerable attention due to their interesting photochemical features that can use as nanocarrier. In this paper, the thermal response of MX and the use of this property for thermal effects during the photodynamic process by MX-conjugated HGN were investigated. Material and Methods: After optimizing the synthesis of ultimate nanostructure, the characteristics of pharmacological agents including MX, HGN, methoxy polyethylene glycol (mPEG)-HGN, and MX-mPEG-HGN were determined. Then, the thermal response of MX was determined at 0-50°C. Finally, by applying light irradiation with a non-coherent source at a wavelength of 670 nm and exposures of 0 to 50 j/cm2, the profile release and temperature variation in MX-mPEG-HGN were determined. Results: The zeta pote...
Introduction: Hollow gold nanoshells (HAuNS) are one of the most attractive nanostructures for bi... more Introduction: Hollow gold nanoshells (HAuNS) are one of the most attractive nanostructures for biomedical applications due to their interesting physicochemical properties. This study sought to evaluate the plasmonic photothermal effect of HAuNS irradiated with incoherent light on melanoma cell line. Materials and Methods: After the synthesis of nanostructures, the temperature changes of HAuNS and polyethylene glycol stabilized HAuNS (HAuNS-PEG) were evaluated at different irradiation dose levels. After determining the potential cytotoxicity of the agents, the DFW cells were irradiated by incoherent light with and without the nanostructures at different exposure doses with two spectral bands of 670±25 nm and 730±25 nm. Finally, the rate of the cell survival was determined by 1-Methyltetrazole-5-Thiol assay 24 h after irradiating. Results: The HAuNS, HAuNS-PEG, and light exposure did not have any significant effect on the cell survival, individually. Stabilizing with PEG led to an inc...
Iranian Journal of Medical Physics, 2020
Objectives: Photochemical internalization (PCI) is a novel PDT-based technology for intracellular... more Objectives: Photochemical internalization (PCI) is a novel PDT-based technology for intracellular delivery of hydrophilic macromolecular therapeutic agents and other drugs with limited ability to penetrate cellular membranes with intracellular targets. One of the approaches is the use of nanoparticles along with photosensitizing agents. In this study, the presence of thioglucose-coated gold nanoparticles in the efficiency of the photodynamic effect of methylene blue due to photochemical internalization phenomenon was investigatedMaterials and Methods: This study was performed on the MCF-7 cell line derived from tumor cells of breast adenocarcinoma. First, GNPs-Tio was synthesized. Next, the toxicity of GNPs-Tio and MB were determined to achieve their optimal concentrations. Then, their photodynamic effects of GNPs-Tio combined with MB by Luma-Care source light at different doses were evaluated by using MTT assay and colony assay (12 days after treatment). Results: According to MTT a...
Photodiagnosis and Photodynamic Therapy
Uploads
Papers by armin imanparast