Paul R Jaschke

Macquarie University, School of Natural Sciences, Faculty Member

University of British Columbia, Microbiology And Immunology, Alumnus

Macquarie University, Chemistry and Biomolecular Sciences, Faculty Member

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I am now a Lecturer (equivalent of Assistant Professor) of Synthetic Biology in the Department of Chemistry and Biomolecular Sciences at Macquarie University in Sydney, Australia.
I started off my science career at the University of Alberta (Canada), working in the Casey and Michalak labs. Summer student work in the Casey Lab on the human sodium-bicarbonate co-transporter protein resulted in an authorship in a peer-reviewed publication. My honors project in the Michalak Lab had me determining the role of calcium signaling and calreticulin on murine embryonic stem cell differentiation. After achieving a B.Sc. (Honors) in Biochemistry in 2003 I went to the Beatty Lab at the University of British Columbia to start my Ph.D. While at UBC I discovered that a photosynthetic organism called Rhodobacter sphaeroides was capable of re-routing its chlorophyll biosynthetic machinery around a blockage to generate a new type of chlorophyll. November 2010 I was conferred a Ph.D in Microbiology and Immunology from UBC. I was a post-doc in Drew Endy's lab at Stanford University from Nov 2010 - June 2015. The focus of my project was on redesign phage using both rational and combinatorial methods in order to finish the genetics of a simple system.
Supervisors: Drew Endy and Andrew Hessel
Phone: 650-721-5884

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Thomas Riera

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Simulated Sandwich Enzyme-Linked Immunosorbent Assay for a Cost-Effective Investigation of Natural and Engineered Cellular Signaling Pathways S

The ability to separate, identify, and quantify proteins from complex mixtures are key foundation... more The ability to separate, identify, and quantify proteins from complex mixtures are key foundational methods across biochemistry teaching and research. In particular, enzymelinked immunosorbent assay (ELISA) is an important technique that is used to measure antigen concentrations in both industry and academia. There are four categories of ELISA, direct, indirect, competitive, and sandwich, each with their own applications. Sandwich ELISAs are used to determine antigen concentrations from complex mixtures of protein, such as a cell lysates, and are regularly used as medical diagnostics to diagnose illness and diseases ranging from hepatitis to celiac disease. One major problem with teaching the sandwich ELISA technique to students is the prohibitive cost due to the need to coat a 96-well plate with a capture antibody. One solution to this problem would be to significantly reduce the role of each student in the lab, but this does not adequately prepare students to perform the procedure in a research or industry lab. Instead, this laboratory exercise teaches students the procedural knowledge needed to perform a direct sandwich ELISA, but uses a simulated experience performed within a wet-lab environment. The presented scenario is the analysis of phosphorylated proteins within a synthetic signaling pathway, but because the lab uses simulated samples, it can be tailored to different topics and educational aims. The procedure is 10-to 26-fold less expensive per student to deploy than an authentic sandwich ELISA. Students in the course report that the ELISA lab significantly strengthened the connection between theory and practice.

Paul R Jaschke

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