Oxidized model membranes have differential effects on peptide fibril formation, driven by surface... more Oxidized model membranes have differential effects on peptide fibril formation, driven by surface attraction, peptide charge and secondary structure stabilization.
The self-assembly of peptides into supramolecular fibril structures has been linked to neurodegen... more The self-assembly of peptides into supramolecular fibril structures has been linked to neurodegenerative diseases such as Alzheimer’s disease but has also been observed in functional roles. Peptides are physiologically exposed to crowded environments of biomacromolecules, and particularly membrane lipids, within a cellular milieu. Previous research has shown that membranes can both accelerate and inhibit peptide self-assembly. Here, we studied the impact of biomimetic membranes that mimic cellular oxidative stress and compared this to mammalian and bacterial membranes. Using molecular dynamics simulations and experiments, we propose a model that explains how changes in peptide-membrane binding, electrostatics, and peptide secondary structure stabilization determine the nature of peptide self-assembly. We explored the influence of zwitterionic (POPC), anionic (POPG) and oxidized (PazePC) phospholipids, as well as cholesterol, and mixtures thereof, on the self-assembly kinetics of the...
Supporting results The formation of self-assembled monolayers (SAMs) of alkanethiols on gold-coat... more Supporting results The formation of self-assembled monolayers (SAMs) of alkanethiols on gold-coated quartz sensors was checked by measuring the contact angles of water droplets. This provides a measure of the hydrophilicity of the different surfaces.
Oxidized model membranes have differential effects on peptide fibril formation, driven by surface... more Oxidized model membranes have differential effects on peptide fibril formation, driven by surface attraction, peptide charge and secondary structure stabilization.
The self-assembly of peptides into supramolecular fibril structures has been linked to neurodegen... more The self-assembly of peptides into supramolecular fibril structures has been linked to neurodegenerative diseases such as Alzheimer’s disease but has also been observed in functional roles. Peptides are physiologically exposed to crowded environments of biomacromolecules, and particularly membrane lipids, within a cellular milieu. Previous research has shown that membranes can both accelerate and inhibit peptide self-assembly. Here, we studied the impact of biomimetic membranes that mimic cellular oxidative stress and compared this to mammalian and bacterial membranes. Using molecular dynamics simulations and experiments, we propose a model that explains how changes in peptide-membrane binding, electrostatics, and peptide secondary structure stabilization determine the nature of peptide self-assembly. We explored the influence of zwitterionic (POPC), anionic (POPG) and oxidized (PazePC) phospholipids, as well as cholesterol, and mixtures thereof, on the self-assembly kinetics of the...
Supporting results The formation of self-assembled monolayers (SAMs) of alkanethiols on gold-coat... more Supporting results The formation of self-assembled monolayers (SAMs) of alkanethiols on gold-coated quartz sensors was checked by measuring the contact angles of water droplets. This provides a measure of the hydrophilicity of the different surfaces.
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Papers by Tiara Dealey