Michael Norton

Marshall University, Chemistry, Faculty Member

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Ayse Asatekin

Tufts University

Antonio Pantaleo

Università degli Studi di Bari

Tobias Takas Shumba

Harare Polytechnic

Dr.Yousery Sherif

Mansoura University

Irina Kolesnik

Moscow State University

Tania C Cordova- Sintjago

Santa Fe College

Vijay Pande

Stanford University

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Al-Baha University, Kingdom of Saudi Arabia

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Northeastern University

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University of Chicago

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Papers by Michael Norton

Recent advances in molecular lithography

Proceedings of SPIE, Sep 26, 2007

The precise placement of molecular and nanoparticle species at predetermined locations on a subst... more

DNA origami platform development for the detection of metals in water

Proceedings of the West Virginia Academy of Science, Jul 26, 2016

DNA origami platform development for the detection of metals in water. Many people are becoming i... more DNA origami platform development for the detection of metals in water. Many people are becoming increasingly interested in learning the composition of their drinking water. However, for a variety of reasons, only very limited information is available to the public and even to the suppliers of drinking water. Clearly, ubiquitous water testing would be ideal, and this challenge provides motivation for the analytical chemistry community to develop new approaches to quantitation. This project seeks to address this information gap through the development of thin organic arrays, composed of DNA, which are functionalized with molecular species which can selectively bind metals of interest and enable optical detection and characterization of these reversible binding events. Progress in two areas, DNA platform development and ligand design will be presented. Tiles constructed from DNA have been generated which can present a variety of molecular species with a local periodicity of ~100 nm. Modulation of the strength and directionality of interaction of these tiles has been shown to lead to partial or local ordering of these tiles, an important step toward the construction of large scale arrays. Organic ligand systems for two metals of interest, Al and U, have been designed. These ligand designs include two modules, one module enabling covalent attachment to the DNA based platforms through a DNA linker sequence, while the other module supports a binding and reporting function. The synthesis of these ligand systems is currently in progress.

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<title>Ordered DNA arrays prepared via soft lithography</title>

Nanomaterial Synthesis and Integration for Sensors, Electronics, Photonics, and Electro-Optics, 2006

This paper reports progress in an approach to create a general purpose platform to be used in the... more This paper reports progress in an approach to create a general purpose platform to be used in the reproducible assembly of molecular electronic devices. We describe a method in which DNA molecules were immobilized on patterned neutravidin surfaces. First, a silicon wafer was functionalized with (3- aminopropyl)triethoxysilane (APTES) to produce an amine-terminated surface. The primary amine group was then reacted with the heterobifunctional linker molecule succinimidyl-6-(biotinamido)hexanoate which placed an active biotin group at the surface interface. These biotinylated surfaces were then patterned with the tetrameric protein neutravidin using microcontact printing (μCP) with relief features in polydimethylsiloxane (PDMS) stamps. The neutravidin proteins adsorb onto the surface and bind nearly irreversibly to one or two biotin groups leaving at least two biotin binding sites on each protein available for conjugation. Following neutravidin stamping, 1 μm long DNA molecules functionalized on one end with biotin were attached to the patterned areas. Water contact angle (WCA) measurements were used to characterize wettability changes of the silicon surfaces for amine and biotin functionalization. Atomic force microscopy (AFM) was used to image the patterns of immobilized neutravidin and DNA.

Use of Electroluminescence Technology for Highway Signage

... Language: English. Corporate Authors: Nick J. Rahall, II Appalachian Transportation Institute... more

Two-Dimensional Materials as Substrates for the Development of Origami-Based Bionanosensors

IEEE Transactions on Nanotechnology, Sep 1, 2010

In this paper, we report the development of methods for the stable localization of DNA origami as... more In this paper, we report the development of methods for the stable localization of DNA origami as bionanostructures for potential molecular optoelectronic sensor systems. The microcontact printing approach has been adapted to provide a rapid method for the production of self-assembled monolayer nanopatterning of aminopropyltriethoxysilane (APTES) on mica substrates. 1.2 µm blazed gratings were used as molds in the production of soft polydimethylsiloxane stamps. This stamp has been used to prepare fine-line patterns of APTES on flat mica surfaces. A solution-phase method was used to coat the bare mica between these APTES lines with dichlorodimethylsilane, which suppresses nonspecific binding of DNA origami nanostructures. Imaging studies confirm the addressing of rectangular DNA origami structures to the APTES patterns on mica.

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From Nonfinite to Finite 1D Arrays of Origami Tiles

Accounts of Chemical Research, May 6, 2014

Towards DNA methylation detection using biosensors

by Michael Norton and Fariba Esmaeili

The Analyst, 2016

DNA methylation, a stable and heritable covalent modification which mostly occurs in the context ... more

Point-of-care cancer diagnostic devices: From academic research to clinical translation

Talanta

Early and timely diagnosis of cancer plays a decisive role in appropriate treatment and improves ... more Early and timely diagnosis of cancer plays a decisive role in appropriate treatment and improves clinical outcomes, improving public health. Significant advances in biosensor technologies are leading to the development of point-of-care (POC) diagnostics, making the testing process faster, easier, cost-effective, and suitable for on-site measurements. Moreover, the incorporation of various nanomaterials into the sensing platforms has yielded POC testing (POCT) platforms with enhanced sensitivity, cost-effectiveness and simplified detection schemes. POC cancer diagnostic devices provide promising platforms for cancer biomarker detection as compared to conventional in vitro diagnostics, which are time-consuming and require sophisticated instrumentation, centralized laboratories, and experienced operators. Current innovative approaches in POC technologies, including biosensors, smartphone interfaces, and lab-on-a-chip (LOC) devices are expected to quickly transform the healthcare landscape. However, only a few cancer POC devices (e.g. lateral flow platforms) have been translated from research laboratories to clinical care, likely due to challenges include sampling procedures, low levels of sensitivity and specificity in clinical samples, system integration and signal readout requirements. In this review, we emphasize recent advances in POC diagnostic devices for cancer biomarker detection and discuss the critical challenges which must be surmounted to facilitate their translation into clinical settings.

Preparation and enhanced supercapacitance performance of carbonized silk by feeding silkworms MoO2 nanoparticles

Materials & Design

Abstract As a key building block to power flexible electronics, there is need for the development... more Abstract As a key building block to power flexible electronics, there is need for the development of flexible current collectors. Bombyx mori silk, a traditional textile, attracts increasing attention for its potential application in flexible electronics and is a promising raw material for flexible carbon electrodes in current collector fabrication. In this work, molybdenum dioxide nanoparticles (MoO2 NPs), which possess chemical stability and high mass specific capacitance, was used as a feed additive and combined with mulberry leaves to raise silkworms. It was found that the MoO2 NPs were embedded within the silk fibers, which endowed the resulting silk with favorable features for electrochemical energy storage in flexible electronics. After carbonization, the specific capacitance of electrodes prepared with silk from the MoO2 NPs feeding group, with a mass/volume ratio of 5 g/L, was enhanced from 100 F/g to 245 F/g at a current density of 0.2 A/g. Impressively, the electrodes show high coulombic efficiency with excellent stability. This work demonstrates the possibility of producing carbonized silk with superior properties for developing silk-based flexible electrodes for use in energy storage devices in a large-scale, green, low-cost way.

A simple instrument for determining superconducting transition temperatures

Journal of Physics E: Scientific Instruments, 1986

... References Clement J R and Quinnell EH 1952 The low temperature characteristics of carbon-com... more

Aspects of the crystal growth of the intergrowth tungsten bronzes

Solid State Ionics, 1986

Abstract The crystal growth of a few of the members of the family of the rubidium intergrowth tun... more Abstract The crystal growth of a few of the members of the family of the rubidium intergrowth tungsten bronzes has been investigated as part of a program to study the compositionally induced metal to insulator transition in this bronze system. Progress in the study of this nonstoichiometric material has been made possible by successful growth of large single crystal samples. Application of vapor-assisted seeded crystal growth has led to an increase of the product crystal size by two orders of magnitude, from 0.1 mm on an edge to about 2 cm on an edge. Scanning electron microscopy has been used to characterize cracks, voids and intergrowths in this material. Transmission electron microscopy has been used to image the lattice of this quasi-two dimensional material in order to observe the frequency and amplitude of stacking defects.

Site Specific Immobilization of SWCNT onto Single and One Dimensional DNA Origami

The electron-phonon mechanism for resistivity in the high-temperature superconductor system Ba1 - xKxBiO3

We investigate the temperature-dependent resistivity p ( T ) of single crystals of the hightemper... more We investigate the temperature-dependent resistivity p ( T ) of single crystals of the hightemperature superconducting compound Bao.6K,4Bi03 with a critical temperature T,= 30 K, over the temperature range 4.2-300 K. The experimental data are well approximated by a function p f ( T ) of Bloch-Gruneisen type, which we obtain within the classical model of the electron-phonon interaction by assuming that the phonon spectrum consists of an acoustic branch with a Debye dispersion law and a narrow optical-phonon peak located a large distance away.

Feeding Silkworms with HPMC Dispersed MoO2 NPs: An Efficient Strategy to Enhance the Supercapacitance Performance of Carbonized Silk

Composites Science and Technology

A review on nanomaterial-based field effect transistor technology for biomarker detection

Microchimica Acta

Field effect transistor (FET) based sensors have attractive features such as small size, ease of ... more Field effect transistor (FET) based sensors have attractive features such as small size, ease of mass production, high versatility and comparably low costs. Over the last decade, many FET type biosensors based on various nanomaterials (e.g. silicon nanowires, graphene, and transition metal dichalcogenides) have been developed to detect various classes of biomolecular targets due to their integration into portable and rapid test systems, both for use in the clinical lab and in point-of-care testing. This review (with 197 refs.) starts with an introduction into the specific features of FET biosensor technology. This is followed by a description of the essentials of methods for immobilization of recognition elements. The next section discusses the progress that has been made in FET based biosensors using semiconducting nanostructures composed of silicon, graphene, metal oxides, and transition metal dichalcogenides. A further section is devoted to microfluidic systems combined with FET biosensors. We then emphasize the biosensing applications of these diagnostic devices for analysis of clinically relevant biomarkers, specifically to sensing of neurotransmitters, metabolites, nucleic acids, proteins, cancer and cardiac biomarkers. Two tables are presented which summarize advances in applications of 1D and 2D nanomaterial-based FETs for biomarker sensing. A concluding section summarizes the current status, addresses current challenges, and gives perspective trends for the field. Graphical abstract Field effect transistor devices based on the use of 1D and 2D semiconductor nanostructures (so called nano-FETs) are making use of materials including silicon nanowires, graphene, zinc oxide, indium oxide, titanium oxide, and molybdenum disulfide that are further modified with recognition elements for biosensing application. Field effect transistor devices based on the use of 1D and 2D semiconductor nanostructures (so called nano-FETs) are making use of materials including silicon nanowires, graphene, zinc oxide, indium oxide, titanium oxide, and molybdenum disulfide that are further modified with recognition elements for biosensing application.

Layered graphene-mica substrates induce melting of DNA origami

Materials Research Express

Monolayer graphene supported on mica substrates induce melting of cross-shaped DNA origami. This ... more Monolayer graphene supported on mica substrates induce melting of cross-shaped DNA origami. This behavior can be contrasted with the case of origami on graphene on graphite, where an expansion or partially reorganized structure is observed. On mica, only well-formed structures are observed. Comparison of the morphological differences observed for these probes after adsorption on these substrates provides insights into the sensitivity of DNA based nanostructures to the properties of the graphene monolayer, as modified by its substrate.

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Submillimeter-Wave Integrated Micro-Resonators for Investigation of the Dynamical Properties of Biological Molecules

Abstract : The development of a precision measurement technique for determining the complex diele... more Abstract : The development of a precision measurement technique for determining the complex dielectric constant (or permittivity) of biological molecules in the submillimeterwave range from 100 GHz to 1000 GHz. Research Goals: To demonstrate a high-Q submillimeter-wave micro-resonator with sufficient sensitivity to a deposited biological molecule sample to enable precise calculation of the complex dielectric constant. Then to utilize the micro-resonators to collect data on an array of biological molecules ranging from simple to more complex structures.

DNA Origami Reorganizes upon Interaction with Graphite: Implications for High-Resolution DNA Directed Protein Patterning

Nanomaterials (Basel, Switzerland), Jan 31, 2016

Although there is a long history of the study of the interaction of DNA with carbon surfaces, lim... more Although there is a long history of the study of the interaction of DNA with carbon surfaces, limited information exists regarding the interaction of complex DNA-based nanostructures with the important material graphite, which is closely related to graphene. In view of the capacity of DNA to direct the assembly of proteins and optical and electronic nanoparticles, the potential for combining DNA-based materials with graphite, which is an ultra-flat, conductive carbon substrate, requires evaluation. A series of imaging studies utilizing Atomic Force Microscopy has been applied in order to provide a unified picture of this important interaction of structured DNA and graphite. For the test structure examined, we observe a rapid destabilization of the complex DNA origami structure, consistent with a strong interaction of single-stranded DNA with the carbon surface. This destabilizing interaction can be obscured by an intentional or unintentional primary intervening layer of single-stran...

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Towards DNA methylation detection using biosensors

The Analyst, 2016

DNA methylation, a stable and heritable covalent modification which mostly occurs in the context ... more

Methods, probes, and accessory molecules for detecting single nucleotide polymorphisms

Recent advances in molecular lithography

Proceedings of SPIE, Sep 26, 2007

The precise placement of molecular and nanoparticle species at predetermined locations on a subst... more

DNA origami platform development for the detection of metals in water

Proceedings of the West Virginia Academy of Science, Jul 26, 2016

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<title>Ordered DNA arrays prepared via soft lithography</title>