Papers by Debananda Ningthoujam
Current Agriculture Research Journal, Sep 20, 2023
PubMed, Dec 1, 2021
Normal proteins, if misfolded, form insoluble aggregates or fibrils, resistant to proteolytic deg... more Normal proteins, if misfolded, form insoluble aggregates or fibrils, resistant to proteolytic degradation. Such proteins, named amyloid proteins, share a common structural motif, called the cross β sheet structure. The insoluble β‐amyloid fibrils have been implicated in neurodegenerative diseases such as Alzheimer’s disease (AD). Prions have structural similarities with β‐amyloid fibrils. As microbial keratinases can degrade prions, we investigated the possible capacity of microbial keratinases, purified in our lab, for degrading β‐ amyloid fibrils.
Research Journal of Microbiology, May 1, 2008
A bacterium was isolated from garden soil in basal salts medium containing p-nitrophenol (PNP). S... more A bacterium was isolated from garden soil in basal salts medium containing p-nitrophenol (PNP). Subsequent subcultures in agar, nutrient agar plates and agar slants by streaking led to isolation of pure colonies. The pure culture could degrade up to 300 mg/L PNP in ...
Frontiers in Psychology, 2021
Journal of Bacteriology & Mycology: Open Access, 2017
Endophytic bacteria isolated from indigenous medicinal plant Solanum xanthocarpum were screened f... more Endophytic bacteria isolated from indigenous medicinal plant Solanum xanthocarpum were screened for antimycobacterial activities against Mycobacterium smegmatis using MTT assays. Of 18 isolates obtained 3 showed antimycobacterial activity. The crude extract of 3 bioactive isolates were tested against attenuated Mycobacterium bovis strain and pathogenic strain of Mycobacterium tuberculosis and their IC50 values were calculated. Crude metabolites of 3 strains showed IC50 values less than 100µg/ml. The 2 bioactive strains were identified as Streptomyces sp. and other as Bulkholderia fungorum.
Frontiers in Anti-infective Agents, 2019
African Journal of Microbiology Research, Nov 30, 2009
Several actinomycete isolates obtained from various habitats in Manipur were screened for activit... more Several actinomycete isolates obtained from various habitats in Manipur were screened for activity against some major rice fungal pathogens such as Curvularia oryzae MTCC 2605, Pyricularia oryzae MTCC 1477, Bipolaris oryzae LSMU1, and Fusarium oxysporum MTCC 287. LSCH-10C, NRP1-14, NRP1-18 and NRP1-26 showed potent antagonistic activities in dual culture assay. Among 33 indigenous actinomycete isolates, LSCH-10C isolated from Loktak lake sediment on chitin agar, was found most promising to be developed as biocontrol agent (BCA) for rice. The nature of the activity in terms of fungitoxic or fungistatic nature was also determined. This report presents the preliminary results of these bio-control actinomycetes. Some of the strains have been selected for further studies towards application as rice BCAs. This paper also deals with characterization of the most promising agroactive strain.
Extremophiles in Eurasian Ecosystems: Ecology, Diversity, and Applications, 2018
The quest for increasing agricultural production for the burgeoning human population had been eff... more The quest for increasing agricultural production for the burgeoning human population had been effective with the use of nitrogen-based fertilizers. However, its prolong use and occurrence of acid rain resulted in dropping the soil pH below 5.0, whose environmental conditions considerably decreased the beneficial effects of soil neutrophilic bacteria while increasing the abundance of pathogenic fungi. Furthermore, the use of pesticide and synthetic fertilizers had adverse effect on human health and environment. An alternative method will therefore rely on minor groups of bacteria that can sustain its growth under extreme condition. And particularly for designing products to be applied in acidic soil, acidophilic and/or acidotolerant Actinobacteria having antifungal and/or plant growth-promoting activities had tremendous potential for developments as novel biocontrol and/or biofertilizer products. As Actinobacteria can survive under many adverse environment conditions by forming spores, they can be promising bio-agents for sustainable agricultural production. Actinobacteria may help in the degradation of organic matter into humus and release of nitrogen, carbon, and ammonia, in turn supplying the nutrients to agricultural crops in acidic soil. Release of ammonia due to decomposition of chitin by chitinase-producing Actinobacteria may raise the pH of soils, paving a way for other neutrophilic plant growth-promoting bacteria.
Frontiers in Anti-infective Agents, 2019
Alzheimer's & Dementia, 2015
Background: Neurological disorders represent one of the leading causes of disability globally.in ... more Background: Neurological disorders represent one of the leading causes of disability globally.in the world population. They are currently incurable and these diseases worsen over time. Determination of the etiological factor of most of the neurological disorders is confusing as most of these diseases have multifactorial etiology. Some common neurodegenerative disorders such as Alzheimer’s and Parkinson’s diseases are apparently caused by deposition of amyloid plaques. Hence dissolution of these amyloid plaques can be a promising therapeutic approach for of these debilitating disorders. The research laboratory has identified several actinomycete in the last few years and they are being tested for their therapeutic uses. One such interesting strain is Amycolatopsis sp. MBRL 40 which is found to have promising role in amyloidogenic disorders. Methods: Two potent keratinases, Ker 1 and Ker 2, purified from the actinomycete Amycolatopsis sp. MBRL 40 showed potent capacity to degrade amyloid fibrils. These enzymes were purified by subjecting the fermentation broth of the microbial culture to ammonium sulphate fractionation, dialysis and Q sepharose column chromatography. The enzymes were tested for their possible amyloid fibril degrading activity. The amyloid fibrils were induced by incubating lysozyme with 8M urea and the presence of cross beta pleated sheets in these fibrils were confirmed by Congo Red absorption spectroscopy by staining the fibrils with Congo Red and following the absorbance spectra from 400 nm to 700 nm. Results: Both Ker 1 andKer 2 could degrade chicken feather, soluble keratin and casein. Both enzymes showed optimum activity at 40 C and pH 7.0. Ker 1 was found to be more potent than Ker 2. Both were found to be serine proteases. Ker 1 degraded amyloid fibrils within 24 hours at 37 C at a concentration of 125 mg/ml. Ker 2 was found to be less potent than Ker 1 in degrading amyloid fibrils. Conclusions: Ker I and Ker 2 have good potential to be tested on amyloid fibrils on other amyloidogenic neurodegenerative disorders to explore the biotechnological potential of these actinomycete keratinases.
Research Journal of …, 2009
The University of California prohibits discrimination or harassment of any person on the basis of... more The University of California prohibits discrimination or harassment of any person on the basis of race, color, national origin, religion, sex, gender identity, pregnancy (including childbirth, and medical conditions related to pregnancy or childbirth), physical or mental disability, medical condition (cancer-related or genetic characteristics), ancestry, marital status, age, sexual orientation, citizenship, or service in the uniformed services (as defined by the Uniformed Services Employment and Reemployment Rights Act of 1994: service in the uniformed services includes membership, application for membership, performance of service, application for service, or obligation for service in the uniformed services) in any of its programs or activities. University policy also prohibits reprisal or retaliation against any person in any of its programs or activities for making a complaint of discrimination or sexual harassment or for using or participating in the investigation or resolution process of any such complaint. University policy is intended to be consistent with the provisions of applicable State and Federal laws. Inquiries regarding the University's nondiscrimination policies may be directed to the Affirmative
AFRICAN JOURNAL OF BIOTECHNOLOGY, 2012
p-Nitrophenol (PNP), a major nitroaromatic xenobiotic is released into the environment as a resul... more p-Nitrophenol (PNP), a major nitroaromatic xenobiotic is released into the environment as a result of its widespread use and as a breakdown product of organophosphate (OP) agricultural pesticides such as parathion and methyl parathion. It is highly toxic to soil microflora and other non-target organisms. Of several Microbial Biotechnology Research Laboratory (MBRL) strains isolated from limestone deposits in Ukhrul, Manipur, India, two strains, (HS4-2 and HS6-1) were found to be promising PNP biodegraders, degrading up to 30 mg/L PNP in neutral basal salts medium (BSM, pH 7.0). Higher strength alkaline BSM (5X BSM, pH 8.0) was found to be more favorable for PNP degradation by these strains. Under these more optimal conditions, HS4-2 and HS6-1 could degrade up to 270 and 350 mg/L PNP respectively, remarkably high concentrations as PNP is quite toxic even at low concentrations. Only a few bacterial strains degrading such high concentrations of PNP have been reported. As these strains seem promising, they were subjected to phenotypic and genotypic characterization. The phenotypic characteristics of these strains were typical of actinomycetes. 16S recombinant deoxyribonucleic acid (rDNA) sequence analyses indicated strain HS6-1 to have the highest level of sequence similarity with Rhodococcus triatomae IMMIB RIV-085(T)/AJ854055 (98.28%) and HS4-2 with Brevibacterium casei NCDO2048 (T)/X76564 (99.37%). Strain HS6-1 may possibly be a novel species of Rhodococcus. Further studies on optimization, kinetics and pathways of biodegradation can improve the biodegrading capacities of these actinomycete strains.
Journal of Pharmacy Research
Abstract: Falling in the Indo-Burma biodiversity hotspot, Manipur is a promising area for microbi... more Abstract: Falling in the Indo-Burma biodiversity hotspot, Manipur is a promising area for microbial diversity studies. As a part of our ongoing research on actinomycete diversity of Manipur, sediments from various depths of the Loktak Lakethe largest freshwater lake in ...
American Journal of Biochemistry and Biotechnology, 2009
Problem statement: The exhaustion of the usual terrestrial sources and the rise of resistant path... more Problem statement: The exhaustion of the usual terrestrial sources and the rise of resistant pathogens dictate the search for novel actinomycetes and new antibiotics. In this context, niche habitats such as caves, pristine forests, lakes, rivers, and other wetlands, high salt environments, marine ecosystems and endophytic niches are promising targets for survey of bioactive actinomycetes. Approach: Actinomycetes were isolated from several niche habitats in Manipur, India, on selective media such as SCNA and Chitin agar with or without antibiotics. Selected isolates were subjected to antimicrobial activity screening by Kirby-Bauer method. Results: 172 lake sediment (SCNA, LS1 series), 35 lake sediment (CA, LSCH series), 120 river (NRP, NRB and..series), 39 forest (AML series), 35 cave (KC1 series), 101 salt spring (NH, N3S and .. series), 46 Shirui jungle (SJ series) and 66 Shirui hill (SH series) actinomycetes isolates were obtained. Of 99 randomly selected isolates screened, 37 had antimicrobial activities against 1 or more indicator strains: 32 against Gram positive bacteria and 8 against Gram negative bacteria; 10 actinomycete strains were antimycotic and 3 had broad-spectrum antibiotic activities. About 18 potent antibacterial, 1 anti pseudomonas, 1 exclusively antifungal and 3 broad-spectrum antimicrobial actinomycetes were chosen for further studies. Conclusion: Niche habitats in Manipur especially wetlands show great promise for discovery of bioactive actinomycetes.
K Tamreihao 1,2 *, Salam Nimaichand 1,3 , Shamjetshabam Babeeta Chanu 1,4 , Khaidem Aruna Devi 1 ... more K Tamreihao 1,2 *, Salam Nimaichand 1,3 , Shamjetshabam Babeeta Chanu 1,4 , Khaidem Aruna Devi 1 , Rajkumari Lynda 1,5 , Ningthoukhongjam Jeeniita 1 , Debananda S Ningthoujam 1 * & Subhra Saikat Roy Microbial Biotechnology Research Laboratory, Department of Biochemistry, Manipur University, Canchipur-795 003, Manipur, India 2 ICAR Research Complex for NEH Region, Manipur Centre, Imphal-795004, India State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, College of Ecology and Evolution, Sun Yat-Sen University, Guangzhou, Guangdong, China Protein Biochemistry Laboratory, Department of Biochemistry, Manipur University, Canchipur – 795003, India Microbial Resource Centre, Institute of Bioresources and Sustainable Development, Takyelpat, Imphal – 795001, India
A protease producing moderately halo-alkalithermotolerant Bacillus strain SH1 was isolated from s... more A protease producing moderately halo-alkalithermotolerant Bacillus strain SH1 was isolated from salt spring sediment in Manipur, India. Biochemical and physiological characterization and acid production from various carbohydrates by API 50 CHB tests led to its identification as Bacillus subtilis and it was designated as bacillus subtilis strain SH1. Corn starch(1%) and peptone(0.2%) was found as optimal C and N sources for protease production. The enzyme is active over a wide range of temperatures and pH with optima at 50 C and pH 8. It is inhibited by PMSF as well as EDTA and seems to be a metal-activated serine protease or a mixture of enzymes. SH1, interestingly, is stimulated by FeSO4 which has been rarely reported in the literature.
Uploads
Papers by Debananda Ningthoujam