Imran Saleem

Liverpool John Moores University, School of Pharmacy and Biomolecular Sciences, Faculty Member

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My research group transgresses multiple disciplines including formulation, drug delivery, nanotechnology, polymer science, chemistry, cell and molecular biology. The groups work is involved in the application and understanding of novel nanocarrier systems in overcoming biological barriers for the targeted delivery of small molecules and macromolecules (proteins, peptides, siRNA, miRNA) for human and veterinary use via pulmonary and oral delivery.

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Nicole Herbots

Arizona State University

Waltraud M. Kriven

University of Illinois at Urbana-Champaign

PROF. OM PRAKASH SINHA

Amity University

Kaitlyn Dykstra

University of North Carolina at Chapel Hill

ida idayu muhamad

Universiti Teknologi Malaysia - UTM

Sergio Granados

Clark University

Mostafa Moonir Shawrav

Tu Wien

Rafael Abargues

Universitat de València

Anne Bruton

University of Southampton

Virginie Boucher

CSIC (Consejo Superior de Investigaciones Científicas-Spanish National Research Council)

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Papers by Imran Saleem

Corrigendum to “PEG-4000 formed polymeric nanoparticles loaded with cetuximab downregulate p21 & stathmin-1 gene expression in cancer cell lines” [Life Sci. 295 (2022) 120403]

Life Sciences

PEG-4000 formed polymeric nanoparticles loaded with cetuximab downregulate p21 & stathmin-1 gene expression in cancer cell lines

Life Sciences, 2022

Cetuximab (CTX) is known to have cytotoxic effects on several human cancer cells in vitro; howeve... more Cetuximab (CTX) is known to have cytotoxic effects on several human cancer cells in vitro; however, as CTX is poorly water soluble, there is a need for improved formulations can reach cancer cells at high concentrations with low side effects. We developed (PEG-4000) polymeric nanoparticles (PEGNPs) loaded with CTX and evaluated their in vitro cytotoxicity and anticancer properties against human lung (A549) and breast (MCF-7) cancer cells. CTX-PEGNPs were formulated using the solvent evaporation technique, and their morphological properties were evaluated. Further, the effects of CTX-PEGNPs on cell viability using the MTT assay and perform gene expression analysis, DNA fragmentation measurements, and the comet assay. CTX-PEGNP showed uniformly dispersed NPs of nano-size range (253.7 ± 0.3 nm), and low polydispersity index (0.16) indicating the stability and uniformity of NPs. Further, the zeta potential of the preparations was -17.0 ± 1.8 mv. DSC and FTIR confirmed the entrapping of CTX in NPs. The results showed IC50 values of 2.26 μg/mL and 1.83 μg/mL for free CTX and CTX-PEGNPs on the A549 cancer cell line, respectively. Moreover, CTX-PEGNPs had a lower IC50 of 1.12 μg/mL in MCF-7 cells than that of free CTX (2.28 μg/mL). The expression levels of p21 and stathmin-1 were significantly decreased in both cell lines treated with CTX-PEGNPs compared to CTX alone. The CTX-PEGNP-treated cells also showed increased DNA fragmentation rates in both cancer cell lines compared with CTX alone. The results indicated that CTX-PEGNP was an improved formulation than CTX alone to induce apoptosis and DNA damage and inhibit cell proliferation through the downregulation of P21 and stathmin-1 expression.

Particle Size Analysis: from Microparticles to Nanosystems

Spicing Up the Lungs: Design and Characterization of Dry Powder Formulations of Curcumin for Lung Delivery

Functional Nanomaterials

Drug Delivery Using Nanomaterials, 2021

Rationale for Pulmonary Vaccine Delivery: Formulation and Device Considerations

Micro and Nanotechnology in Vaccine Development, 2017

Delivery of vaccines via the pulmonary route has been shown to induce mucosal and systemic immuni... more Delivery of vaccines via the pulmonary route has been shown to induce mucosal and systemic immunity. In addition, these important disease prevention and control agents can be formulated and administered as dry powders, which can overcome the cold-chain and medical personnel requirements. Furthermore, vaccines can be formulated within particulate delivery systems, allowing for enhanced interaction, targeting, and uptake with antigen presenting cells and initiating an enhanced immune response. This chapter outlines the rationale and challenges of targeting the lung antigen presenting cells to initiate an immune response, micro- and nanoparticulate delivery systems with focus on engineering dry powders for inhalation, and pulmonary devices for administration. In addition, some recent case studies are presented, indicating the rationale for pulmonary vaccine delivery compared with other routes of delivery and the importance of device design in pulmonary vaccine administration.

Insight into inclusion complexation of indomethacin nicotinamide cocrystals

Journal of Inclusion Phenomena and Macrocyclic Chemistry, 2016

The version presented here may differ from the published version or from the version of the recor... more

Imran Saleem

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