Papers by Andrei Surguchov
Current Genetics, 1984
A plasmid, pYsup1-1, containing a DNA fragment able to suppress the recessive mutant phenotype of... more A plasmid, pYsup1-1, containing a DNA fragment able to suppress the recessive mutant phenotype of the suppressor locus sup1 (allele sup1-ts36) of Saccharomyces cerevisiae was isolated from a bank of yeast chromosomal DNA cloned in cosmid p3030. The complementing gene was localized on a 2.6 kb DNA fragment by further subcloning. Evidence is presented that the cloned DNA segment codes
Trends in Biochemical Sciences, 1987
ABSTRACT
Molecular and General Genetics, 1980
The shift of recessive suppressor mutant of yeast Saccharomyces cerevisiae from permissive to res... more The shift of recessive suppressor mutant of yeast Saccharomyces cerevisiae from permissive to restrictive conditions is accompanied by polysome decay and accumulation of 80 S ribosomes (Smirnov et al., 1976). In this paper some properties of 80 S ribosomes are studied. It is demonstrated that polysome decay under non-permissive conditions is not the consequence of the impairement of RNA synthesis.
Molecular and General Genetics, 1978
The ribosomal protein patterns of recessive suppressor strain and parent strain of Saccharomyces ... more The ribosomal protein patterns of recessive suppressor strain and parent strain of Saccharomyces cerevisiae were analyzed by two-dimensional polyacrylamide gel electrophoresis. About 30 protein spots were found for ribosomal proteins of small subunit for both mutant and parent strain. These patterns do not differ from each other neither in intensity of staining, nor in mobility of spots. 41 protein spots
Molecular and General Genetics, 1982
Recessive suppressor mutations in yeast Saccharomyces cerevisiae alter a component of the cytopla... more Recessive suppressor mutations in yeast Saccharomyces cerevisiae alter a component of the cytoplasmic ribosomes, relaxing the control of translational fidelity. As a consequence ribosomes can misread nonsense codons as amino acids (Surguchov et al. 1980a).
Gene, 1988
A nucleotide sequence of the yeast Saccharomyces cerevisiae omnipotent suppressor SUP2 (SUP35) ge... more A nucleotide sequence of the yeast Saccharomyces cerevisiae omnipotent suppressor SUP2 (SUP35) gene is presented. The sequence contains a single open reading frame (ORF) of 2055 bp, which may encode a 76.5-kDa protein. A single transcript of 2.3 kb corresponding to a complete ORF is found. Analysis of codon bias suggests that the SUP2 gene is not highly expressed. The C-terminal part of the deduced amino acid sequence shows a high homology to yeast elongation factor EF-1 alpha, whereas the N-terminal part is unique for the SUP2 protein. The N terminus contains a number of short repeating elements and possesses an unusual amino acid composition. Analysis of the nucleotide and deduced amino acid sequences indicates that three additional proteins could possibly be expressed, two of which might be initiated on internal ATG codons and a third might be formed by alternative splicing. One of these proteins is supposed to be imported into mitochondria. Possible functions of the SUP2 gene product(s), especially its putative activity as a soluble factor controlling the fidelity of translation, are discussed.
European Journal of Biochemistry, 1975
Using sucrose density centrifugation and gel filtration of a 105000 x g supernatant of Bacillus h... more Using sucrose density centrifugation and gel filtration of a 105000 x g supernatant of Bacillus hvevrs two enzymic activities of glycyl-tRNA synthetase were separated. Enzyme catalyzing the aminoacylation of tRNA (El) elutes in a high-molecular-weight region. Enzyme active in glycylhydroxamate formation (E,) elutes from a Sephadex gel column and sediments in sucrose density gradient in a region of relatively low molecular weight. The presence of two enzymic activities does not depend on the method of cell disruption; their proportion does not change when protease inhibitor (diisopropylphosphorofluoridate) is added to the extraction buffer.
FEBS Letters, 1987
Primary structures of yeast sup2 gene and polypeptide product coded by the gene are compared with... more Primary structures of yeast sup2 gene and polypeptide product coded by the gene are compared with the current nucleotide and amino acid sequence data base. The amino acid sequence of the sup2 product shows homology to elongation factors from different sources. Especially high homology is found in the regions, corresponding to conservative aminoacyl-tRNA-and GTP-binding domains, described in elongation factors and other proteins. The data obtained are discussed in relation to the functions of sup2 polypeptide product in protein synthesis. Protein synthesis; GTP-binding site; aminoacyl-tRNA-binding site; Gene homology Published by Elsevier Science Publbhers B. V. (Biomedical Division) 00145793/87/$3.50 0 1987 Federation of European Biochemical Societies 257 Volume 215, number 2 FEBS LETTERS May 1987
FEBS Letters, 1986
The results of Southern blotting demonstrate that sup2 is a unique gene in Saccharomyces cerevisi... more The results of Southern blotting demonstrate that sup2 is a unique gene in Saccharomyces cerevisiae that does not possess homologous sequences in the yeast genome. The direct hybridization of DNA fragments, containing cloned sup1 and sup2 genes, did not reveal any structural homology between these two genes. By Northern blotting analysis the sizes of the transcripts were determined to be 1.6 kb for sup1 gene and 2.5 and 1.4kb for sup2 gene. Experiments with RNA isolated from yeast mutant with impaired splicing demonstrated that sup1 and sup2 genes do not contain introns.
γ-Synuclein (Syn G) is highly expressed in retinal ganglion cells and the loss of these cells in ... more γ-Synuclein (Syn G) is highly expressed in retinal ganglion cells and the loss of these cells in glaucoma is associated with significant reduction of the intracellular Syn G level. However, causative relationship between these two events has not been established. Here we show that the knockdown of Syn G results in a decreased viability of the immortalized retinal ganglion cells
Purpose: Previous studies have described γ-synuclein as a protein highly expressed in retinal gan... more Purpose: Previous studies have described γ-synuclein as a protein highly expressed in retinal ganglion cells (RGCs), and a loss of RGCs correlates with a downregulation of γ-synuclein gene expression in glaucoma. Here we asked whether γ- synuclein expression in the retina can be considered a specific marker of RGCs. Methods: γ-Synuclein expression was examined with immunohistochemistry in retinal sections from
Synucleins are small, soluble proteins expressed primarily in neural tissues and certain tumors. ... more Synucleins are small, soluble proteins expressed primarily in neural tissues and certain tumors. The family includes three known proteins: α‐synuclein, β‐synuclein, and γ‐synuclein. A typical structural feature of synucleins is the presence of a repetitive, degenerative AA motif KTKEGV throughout the first 87 residues and acidic stretches within the C‐terminal region. Members of the synuclein family are natively unfolded proteins
International review of cell and molecular biology, 2008
Synucleins are small, soluble proteins expressed primarily in neural tissues and certain tumors. ... more Synucleins are small, soluble proteins expressed primarily in neural tissues and certain tumors. The family includes three known proteins: alpha-synuclein, beta-synuclein, and gamma-synuclein. A typical structural feature of synucleins is the presence of a repetitive, degenerative AA motif KTKEGV throughout the first 87 residues and acidic stretches within the C-terminal region. Members of the synuclein family are natively unfolded proteins that are characterized by a high net charge and low hydropathy. The synuclein family recently came into the spotlight when one of its members, alpha-synuclein, was linked both genetically and neuropathologically to Parkinson's disease. It has a role in other neurodegenerative diseases, such as dementia with Lewy bodies, multiple system atrophy, neurodegeneration with brain iron accumulation type 1, and Alzheimer's disease. Interestingly, another member of the family, beta-synuclein, possesses antagonistic properties to alpha-synuclein. Th...
Molecular vision, 2008
Previous studies have described gamma-synuclein as a protein highly expressed in retinal ganglion... more Previous studies have described gamma-synuclein as a protein highly expressed in retinal ganglion cells (RGCs), and a loss of RGCs correlates with a downregulation of gamma-synuclein gene expression in glaucoma. Here we asked whether gamma-synuclein expression in the retina can be considered a specific marker of RGCs. gamma-Synuclein expression was examined with immunohistochemistry in retinal sections from normal and glaucomatous human eyes. Primary cultures of RGCs from Sprague-Dawley rats purified by sequential immunopanning using a monoclonal antibody to Thy1-1, cultures of A7 immortalized optic nerve astrocytes from newborn rats, and the immortalized RGC-5 cell line were studied using immunofluorescence and quantitative RT-PCR. gamma-Synuclein was highly expressed in RGCs in the human retina and was localized in cytoplasm adjacent to the RGC nuclear marker, Brn-3a. Axons of RGCs were immunopositive for gamma-synuclein in the nerve fiber layer (NFL), the lamina cribrosa and the ...
Cell motility and the cytoskeleton, 2001
Synucleins are small, highly conserved proteins in vertebrates, especially abundant in neurons an... more Synucleins are small, highly conserved proteins in vertebrates, especially abundant in neurons and typically enriched in presynaptic terminals. alpha-Synuclein protein and a fragment of it, called NAC, have been found in association with pathological lesions of neurodegenerative diseases. Recently, mutations in a alpha-synuclein gene have been reported in families susceptible to an inherited form of Parkinson's diseases. In addition, alpha-synuclein has been implicated in the pathophysiology of other neurodegenerative diseases, including Alzheimer's disease and multiple system atrophy. Far less is known about other members of the synuclein family, beta- and gamma-synucleins. gamma-synuclein is up-regulated in several types of cancer and may affect the integrity of the neurofilament network, while its bovine ortholog, synoretin, activates the Elk-1 signal transduction pathway. In this paper, we present data about the localization and properties of human and bovine gamma-synuc...
Cell Motility and the Cytoskeleton, 2006
g-Synuclein is a member of the synuclein family consisting of three proteins. Within the last sev... more g-Synuclein is a member of the synuclein family consisting of three proteins. Within the last several years increasing attention has focused on these proteins because of their role in human diseases. a-Synuclein relevance to Parkinson's disease is based on mutations found in familial cases of the disease and its presence in filaments and inclusion bodies in sporadic cases. g-Synuclein is implicated in some forms of cancer and ocular diseases, while b-synuclein may antagonize their pathological functions. In this paper we present data on the localization and properties of g-synuclein in several neuronal and nonneuronal cell cultures. We show that contrary to the current opinion, gsynuclein is not an exclusively cytoplasmic protein, but has a dynamic localization and can associate with subcellular structures. It is present in the perinuclear area and may be associated to centrosomes. On late steps of mitosis g-synuclein is not found in the centrosomes, and redistributes to the midbody in telophase. Under stress conditions a translocation of g-synuclein from the perinuclear area to the nucleus occurs exhibiting nucleocytoplasmic shuttling. g-Synuclein overexpression reduces neurite outgrowth in a greater extent then a-synuclein overexpression. These data support the view that g-synuclein may change its intracellular localization and associate with subcellular structures in response to intracellular signaling or stress. Cell Motil.
Journal of Ocular Biology, Diseases, and Informatics, 2010
Retinal ganglion cells apoptosis is linked to matrix metalloproteinase 9 (MMP-9) controlled chang... more Retinal ganglion cells apoptosis is linked to matrix metalloproteinase 9 (MMP-9) controlled changes of extracellular matrix. Abnormal expression of MMP-9 is associated with glaucomatous alterations. Thus, the knowledge of MMP-9 regulation is important for the understanding the pathogenesis of glaucoma. Here, we investigated the role of 3′-untranslated regions (3′-UTR) and microRNAs in MMP-9 regulation. We used in vitro mutagenesis and Luc reporter system to identify regulatory elements in the 3′-UTR of MMP-9. microRNAs were analyzed by qRT-PCR, and their role was investigated with inhibitors and mimics. We identified targets for miRNAs in 3′-UTR of MMP-9 involved in the regulation of MMP-9 expression. We then isolated miRNAs from the optic nerve A7 astrocytes and 293 T cells and confirmed the role of mi340 in the regulation using specific inhibitors and mimics. The results obtained show a new miRNA-mediated mechanism of MMP-9 expression regulation.
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Papers by Andrei Surguchov