Dong Jin Shin

Johns Hopkins University School of Medicine, Biomedical Engineering, Graduate Student

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Ille C. Gebeshuber

Tu Wien

Dr.Mahmoud Lotfy

Minufiya University

Amir Manbachi

Johns Hopkins University

Edgar Goluch

Northeastern University

Md. Shamsul Arefin

Deakin University

Sarah Martin

University of Edinburgh

Michael J. Gonzalez

Universidad de Puerto Rico Rio

Hanaa Ahmed

Animal Health Research Institute

Prof. Tofazzal Islam

Bangabandhu Sheikh Mujibur Rahman Agricultural University

Sharma Balakrishnan

Universiti Sains Islam Malaysia (USIM)

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Papers by Dong Jin Shin

Field emission properties of double-walled carbon nanotubes coated with Ru metal nanoparticles

2009 22nd International Vacuum Nanoelectronics Conference, 2009

Mobile nucleic acid amplification testing (mobiNAAT) for Chlamydia trachomatis screening in hospital emergency department settings

Scientific Reports, 2017

Management of curable sexually-transmitted infections (STI) such as Chlamydia can be revolutioniz... more Management of curable sexually-transmitted infections (STI) such as Chlamydia can be revolutionized by highly sensitive nucleic acid testing that is deployable at the point-of-care (POC). Here we report the development of a mobile nucleic acid amplification testing (mobiNAAT) platform utilizing a mobile phone and droplet magnetofluidics to deliver NAAT in a portable and accessible format. By using magnetic particles as a mobile substrate for nucleic acid capture and transport, fluid handling is reduced to particle translocation on a simple magnetofluidic cartridge assembled with reagents for nucleic acid purification and amplification. A mobile phone user interface operating in tandem with a portable Bluetooth-enabled cartridge-processing unit facilitates process integration. We tested 30 potentially Chlamydia trachomatis (CT)-infected patients in a hospital emergency department and confirmed that mobiNAAT showed 100% concordance with laboratory-based NAAT. Concurrent evaluation by a nontechnical study coordinator who received brief training via an embedded mobile app module demonstrated ease of use and reproducibility of the platform. This work demonstrates the potential of mobile nucleic acid testing in bridging the diagnostic gap between centralized laboratories and hospital emergency departments. Curable sexually transmitted infections still present a major concern for public health. Among them, Chlamydia trachomatis (CT) is one of the most common infectious diseases in the United States 1. Because of the high prevalence of asymptomatic infection 2 and serious complications associated with missed infections, including infertility and ectopic pregnancy 3 , routine screening of all sexually active women under the age 26 years is now recommended as the standard of care 4. Unfortunately, only 38% of potential patients actually receive screening, which is in large part due to a significant deficiency in sensitivity and accessibility for the commercially available chlamydia tests 5. For example, lateral-flow device (LFD) rapid tests based on immunochromatography are easy to use and widely available, but they suffer from alarmingly poor sensitivity at 41–87% 6–8. In contrast, nucleic acid amplification tests (NAATs) are the de facto gold standard assays recommended by the Centers for Disease Control and Prevention (CDC) with >90% clinical sensitivity and 99% specificity 9, 10. However, NAATs remain tied to the clinical laboratory because of the technical challenges associated with implementing the conventional bioassay workflow such as multi-step fluidic processing in a simple and affordable format. Comparative studies suggest that point-of-care (POC) tests that are both sensitive and affordable may substantially change the discourse of chlamydia surveillance 10, 11 , highlighting a major unmet need for a platform technology capable of delivering laboratory-based assays in a POC setting. Here we present a standalone, sample-to-answer mobile NAAT system for chlamydia surveillance, incorporating a droplet magnetofluidic cartridge processed in tandem with a portable electromechanical unit operated by a mobile phone interface. Analyte capture and transport via magnetofluidic manipulation replace conventional

Dong Jin Shin

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