C. elegans CRISPR Knock-In
Precise gene insertion for functional studies and disease modeling
C. elegans CRISPR knock-in enables researchers to insert specific DNA sequences at targeted genomic locations with high efficiency. This technique is ideal for tagging genes, introducing reporters, or mimicking disease-relevant mutations. Our optimized protocols deliver reproducible results, helping you generate transgenic lines with confidence.
🧪 Validated lines | Fast turnaround | Flexible customization
Why Use CRISPR Knock-In?
We are transforming preclinical research with accelerated therapeutic screening and high-throughput transgenic model generation
Genetically Modifiable
Insert DNA sequences at exact loci with single-copy resolution.
Optimized Workflow
Streamlined protocols ensure high editing success and reduced screening burden.
Customization Ready
Design and insert fluorescent tags, functional domains, or point mutations.
End-to-End Delivery
Receive fully validated strains with complete documentation.
Our Knock-in Workflow
1
Design & Strategy
Guide RNA and repair template are tailored to your gene and objective.
2
Microinjection
CRISPR-Cas9 components are injected into C. elegans germline.
3
Screening & Selection
F1 and F2 progeny are screened for correct insertion using PCR and phenotypic markers.
4
Validation
Confirm insertion via sequencing and fluorescence expression (if applicable).
Applications in Research
- Endogenous Protein Tagging
Precisely tag endogenous proteins within their native genomic context to study real-time localization, expression levels, and dynamic behavior across various conditions and developmental stages.
- Reporter Gene Insertion
Introduce reporter genes at specific loci to enable continuous, real-time tracking of gene expression and transcriptional activity, enhancing insights into gene regulation.
- Point Mutations for Disease Modeling
Recreate clinically relevant point mutations to mimic human genetic diseases, allowing for in-depth analysis of functional consequences, disease progression, and therapeutic response.
- Structure-Function Studies
Modify or introduce specific protein domains to dissect gene function, explore structure-activity relationships, and assess the impact of precise alterations on biological processes.
C. elegans Knock-in Service Custom Offerings
Our C. elegans Knock-in service custom offerings provide researchers with tailored solutions for precise genetic modifications in C. elegans.
| Build Type | Full Build | Candidate Lines | Custom Injection Mix |
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Transgenic Design |
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Injections |
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Candidate Screening |
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Strain Confirmation |
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Pricing |
$3,812 and up |
$2,679 and up |
$1,914 and up |
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Timeline |
8 weeks and up |
6 weeks and up |
4 weeks and up |
- Additional charge applies for cargo size above 200bp for all packages.
Service Options
Our integrated platform delivers end-to-end epilepsy modeling services
Fluorescent Tag
Visualize your protein via the addition of a fluorescent protein tag at the native locus.
Immunotag
Use your protein for biochemical studies by adding an immunotag at the native locus.
Degron Tag
Tag your protein of interest for controlled degradation.
Point Mutation
The best gene editing method for creating small, precise edits to introduce a small number of nucleotide changes at a target site.
Whole Gene Humanization
Replace a C. elegans gene with an orthologous gene from human or any other organism.
C. elegans Fluorescent Tagging Service
Add a fluorescent protein tag at the endogenous locus using CRISPR gene editing to study gene expression patterns and protein localization in C. elegans. Ideal for live imaging thanks to the worm’s transparency. What you can achieve:
- Detect expression levels with native gene context
- Match expression patterns to your needs
- Identify protein-protein interactions
- Visualize synthesis rates and quantify expression
- Confirm degradation with degron sequence
- Track protein localization to organelles or membranes
Fluorescent Tag
Choose from optimized options like YFP, BFP, eGFP, mCherry, mOrange, mScarlet, and more. RNAi-based knockdown of native expression is also available.
Tagging Strategy
Need split protein function? We support 2A or SL2 sequences to separate components. Have a custom fluor? Send us the sequence — we’ll integrate it.
Full-Service Support
We handle everything from project design to strain delivery.
- Helpful Links:
- Choosing the right fluorophore
- Best fluorescent proteins by experiment type
- Choosing the best tagging terminus
C. elegans Immunotag Service
Accurately quantify protein levels without disrupting gene expression by integrating immunotags at the endogenous locus using CRISPR/Cas9 technology.
- Precise protein quantification at native expression levels
- No disruption of gene expression or function
- Seamless CRISPR/Cas9-based tag insertion
Tag Options
Choose from rigorously tested tags including: FLAG, HA, HIS, TAP, S-peptide — all optimized for high expression and detection in C. elegans.
Why Use Immunotags?
- Validate antibody-based detection
- Enable easy protein tracking
- Expand experimental flexibility when fluorescent imaging isn’t ideal
- Helpful Links:
- Compare Immunotag vs Fluorescent Tag
- hoosing the Right Epitope Tag
Complementary Services
CRISPR Knock-In Services FAQs
Here are some frequently asked questions about CRISPR knockin.
CRISPR knockin involves introducing a specific DNA sequence into a targeted genomic locus, while CRISPR knockout involves disrupting or deleting a specific gene or genomic locus.
CRISPR knockin involves introducing a specific DNA sequence into a targeted genomic locus, while CRISPR knockout involves disrupting or deleting a specific gene or genomic locus.
CRISPR knockin involves introducing a specific DNA sequence into a targeted genomic locus, while CRISPR knockout involves disrupting or deleting a specific gene or genomic locus.
CRISPR knockin involves introducing a specific DNA sequence into a targeted genomic locus, while CRISPR knockout involves disrupting or deleting a specific gene or genomic locus.
Get in Touch
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