Papers by Dr. Richa Saxena
International Journal of Pharmacy and Pharmaceutical Sciences, 2017
Objective: The aim of the present investigation was to form matrix type transdermal patches conta... more Objective: The aim of the present investigation was to form matrix type transdermal patches containing imipramine hydrochloride were prepared using two polymers by solvent evaporation technique to minimise the dose of the drug for lesser side effect and increase the bioavailability of a drug.Methods: In the present study, drug loaded matrix type transdermal films of imipramine hydrochloride were prepared by the solvent evaporation method with the help of polymers along with polyethene glycol (PEG) 400 was used as plasticizer and dimethyl sulfoxide (DMSO) was used as penetration enhancer. Drug-polymer interactions determine by FTIR and a standard calibration curve of imipramine hydrochloride was determined by using UV estimation.Results: The formulated transdermal patch by using PVP K-30, HPMC K100M showed good physical properties. All prepared formulations indicated good physical stability. The formulation F-1 gave the most suitable transdermal film with all desirable physicochemica...
African Journal of Microbiology Research, Oct 11, 2013
The present work deals with the isolation and characterization of microorganisms from polyhouse a... more The present work deals with the isolation and characterization of microorganisms from polyhouse agriculture soil of Jharna village (Rajasthan). Several bacteria and fungi were isolated from polyhouse soil, using serial dilution method. These bacterial isolates were Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Enterobacter aerogenes, Shigella sp., Proteus mirabilis, Bacillus anthracis, Bacillus subtilis, Staphylococcus aureus and Staphylococcus epidermidis species which were further identified on the basis of colony morphology, Gram staining, biochemical tests and using selective and differential media. Identification of fungi such as Aspergillus niger, Aspergillus flavus, Fusarium oxysporum, Trichoderma sp. and Rhizopus sp. was carried out by culturing on potato dextrose and sabouraud's dextrose agar media and microscopic method. Microorganisms play an important function in biodegradation of solid agriculture waste and also help in the crop production.
Journal of Medicinal Plants Research, 2013
In the present study, leaves of Thuja orientalis were powdered and extracted by soxhlet extractor... more In the present study, leaves of Thuja orientalis were powdered and extracted by soxhlet extractor in two solvent systems that is, (E 1) ethyl acetate: chloroform: ethanol (40: 30: 30) and (E 2) methanol: distilled water (70:30). This study conferred the screening of phytochemical constituents, antioxidant activity and antibacterial activity of crude E 1 and E 2 extract and its fractions. Antioxidant activity was carried out by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The results indicate that E 2 extract (70% methanolic extract) had the highest antioxidant effect (85.25% inhibition) at 100 µg/ml concentration and the crude extracts (E 1 and E 2 extract) showed significant (P ≤ 0.05) inhibitory activity against both gram positive and gram negative organisms. It was active against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Agrobacterium tumefaiens. The minimum inhibitory concentrations (MICs) of E 1 extract ranged from 0.40 to 0.85 mg/ml and E 2 extract 0.55 to 1.15 mg/ml. The highest antibacterial potentiality was exhibited by E 2 extract. The fractions also exhibited antimicrobial activity against all the selected microorganisms. The study revealed that T. orientalis is a promising phytomedicine for antioxidant and antibacterial activity.
International Journal of Pharmacy and Pharmaceutical Sciences, Mar 16, 2014
ABSTRACT Objective: The present study aimed to investigate the qualitative and quantitative analy... more ABSTRACT Objective: The present study aimed to investigate the qualitative and quantitative analysis of the major bioactive constituents of medicinally important plant Mimosa hamata in its ethanolic and methanolic extract of whole parts of plant. Methods: Studies were carried out in terms of methanolic and ethanolic extraction, total extractive values, qualitative and quantitative estimation of phytochemicals. Results: The percentage value of yield extraction in ethanolic extract was 34.74% and methanolic extract was 24.2%. The preliminary phytochemical analysis showed the presence of alkaloids, phytosterols, glycosides, tannins & phenolic compounds, flavonoids, saponins, carbohydrates, protein and amino acids, fixed oils and fat test. The total phenolic content ranged from 280.5 to 287.5 mg/g of dry weight of extract, expressed as Gallic acid equivalents. The total flavonoid concentrations varied from 264.4 to 267.3 mg/g, expressed as Quercetin equivalents. Conclusions: It signifies that results revealed the presence of various bioactive constituents which could be exploited for their potential applications for medicinal purposes.
Xylanase catalyze the hydrolysis of xylan, the major constituent of hemicellulose. Applications o... more Xylanase catalyze the hydrolysis of xylan, the major constituent of hemicellulose. Applications of xylanases can be found in the food, feed and paper industry. The major problem in the field of paper bleaching is the removal of lignin and its derivatives, which are linked to cellulose and xylan. Xylanases are more suitable in paper and pulp industry than lignin degrading systems. This study aimed to find out the most efficient producer of xylanase enzyme isolated from soil samples of Jharna village. Various Aspergillus species were isolated from soil samples. Comparative studies were done to identify the most efficient producer of Xylanase among various species of Aspergillus namely A.niger, A. fumigatus and A. flavus after three days of incubation, at optimum temperature and pH conditions. Wheat substrate provide maximum yield at optimum temperature and pH conditions as compared to rice substrate. Aspergillus fumigatus showed the highest enzyme production in presence of wheat substrate as a carbon source. A. fumigatus showed on wheat substrate as the source of xylan produced highest enzymatic activity, clearing zones formation i.e. 79 mm at 37°C and pH 9 and produced high amount of Xylanase with optical density reading 0.897 at pH 7.4 respectively, thus A. fumigatus produced maximum amount of cellulose free Xylanase.
International Research Journal of Pharmacy, 2017
The present study aimed to evaluate the phenolic compounds and in vitro antioxidant properties of... more The present study aimed to evaluate the phenolic compounds and in vitro antioxidant properties of ethanolic extract and selected fraction of Mimosa hamata whole plant. Phytochemical analysis of the extract of M. hamata indicated the presence of phenols and flavonoids in plant. The highest total phenolic and flavonoid content was observed in the IG fraction of M. hamata (654.33 ± 0.008 mg/g and 689.66 ± 0.032 mg/g respectively) in comparison to other fractions. The present investigation showed that ethanolic extract and fraction of M. hamata at various concentrations have good antioxidant capacity. Therefore, the overall results of the present studies were indicated that these bioactive compounds have been of interest for health benefits, the present analytical study proved a potential application to identify and quantify the phenolic compounds in plant extract and fractions.
Journal of Biological Sciences, 2014
In this study, an ethnomedicinal plant Glycosmis pentaphylla Lour. has been investigated for its ... more In this study, an ethnomedicinal plant Glycosmis pentaphylla Lour. has been investigated for its pharmacological potential including antidiabetic, antioxidant, and cytotoxic activities. In the investigation of antidiabetic activity, diabetic induced rats were received methanol extract repeatedly for consecutive 3 weeks. This treatment with the extracts resulted in a reduction of the blood glucose level to 33.82%, 24.38%, and 9.59% after 7, 14, and 21 days of treatment, respectively. In this case, statistically significant blood glucose lowering potential of methanol leaf extract at a dose of 250 mg/kg body weight (bw) was found. In 2,2-diphenyl-1-picrylhydrazyl radical scavenging assessment, moderate antioxidant activity was observed for crude methanol extract with an IC 50 value of 46.75 µg/ml. In brine shrimp lethality evaluation, the methanol extract showed significant cytotoxicity with an LC 50 value of 22.55 µg/ml. Our study testifies that methanol extract of Glycosmis pentaphylla has promising pharmacological activities and explored these species as a potential source of active secondary metabolites for pharmaceutical and agrochemical industries.
The present study aimed to investigate the qualitative and quantitative analysis of the major bio... more The present study aimed to investigate the qualitative and quantitative analysis of the major bioactive constituents of medicinally important plant Mimosa hamata in its ethanolic and methanolic extract of whole parts of plant.
In the present study, leaves of Thuja orientalis were powdered and extracted by soxhlet extractor... more In the present study, leaves of Thuja orientalis were powdered and extracted by soxhlet extractor in two solvent systems that is, (E 1 ) ethyl acetate: chloroform: ethanol (40: 30: 30) and (E 2 ) methanol: distilled water (70:30). This study conferred the screening of phytochemical constituents, antioxidant activity and antibacterial activity of crude E 1 and E 2 extract and its fractions. Antioxidant activity was carried out by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The results indicate that E 2 extract (70% methanolic extract) had the highest antioxidant effect (85.25% inhibition) at 100 µg/ml concentration and the crude extracts (E 1 and E 2 extract) showed significant (P ≤ 0.05) inhibitory activity against both gram positive and gram negative organisms. It was active against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Agrobacterium tumefaiens. The minimum inhibitory concentrations (MICs) of E 1 extract ranged from 0.40 to 0.85 mg/ml and E 2 extract 0.55 to 1.15 mg/ml. The highest antibacterial potentiality was exhibited by E 2 extract. The fractions also exhibited antimicrobial activity against all the selected microorganisms. The study revealed that T. orientalis is a promising phytomedicine for antioxidant and antibacterial activity.
Xylanase catalyze the hydrolysis of xylan, the major constituent of hemicellulose. Applications o... more Xylanase catalyze the hydrolysis of xylan, the major constituent of hemicellulose. Applications of xylanases can be found in the food, feed and paper industry. The major problem in the field of paper bleaching is the removal of lignin and its derivatives, which are linked to cellulose and xylan. Xylanases are more suitable in paper and pulp industry than lignin degrading systems. This study aimed to find out the most efficient producer of xylanase enzyme isolated from soil samples of Jharna village. Various Aspergillus species were isolated from soil samples. Comparative studies were done to identify the most efficient producer of Xylanase among various species of Aspergillus namely A.niger, A. fumigatus and A. flavus after three days of incubation, at optimum temperature and pH conditions. Wheat substrate provide maximum yield at optimum temperature and pH conditions as compared to rice substrate. Aspergillus fumigatus showed the highest enzyme production in presence of wheat substrate as a carbon source. A. fumigatus showed on wheat substrate as the source of xylan produced highest enzymatic activity, clearing zones formation i.e. 79 mm at 37°C and pH 9 and produced high amount of Xylanase with optical density reading 0.897 at pH 7.4 respectively, thus A. fumigatus produced maximum amount of cellulose free Xylanase.
The present work deals with the isolation and characterization of microorganisms from polyhouse a... more The present work deals with the isolation and characterization of microorganisms from polyhouse agriculture soil of Jharna village (Rajasthan). Several bacteria and fungi were isolated from polyhouse soil, using serial dilution method. These bacterial isolates were Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Enterobacter aerogenes, Shigella sp., Proteus mirabilis, Bacillus anthracis, Bacillus subtilis, Staphylococcus aureus and Staphylococcus epidermidis species which were further identified on the basis of colony morphology, Gram staining, biochemical tests and using selective and differential media. Identification of fungi such as Aspergillus niger, Aspergillus flavus, Fusarium oxysporum, Trichoderma sp. and Rhizopus sp. was carried out by culturing on potato dextrose and sabouraud's dextrose agar media and microscopic method. Microorganisms play an important function in biodegradation of solid agriculture waste and also help in the crop production.
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Papers by Dr. Richa Saxena