prof. Pavol Zubor, MD, PhD, DSc.

It is known that BRCA genes play central roles in hereditary breast and ovarian cancers. BRCA1 mu... more It is known that BRCA genes play central roles in hereditary breast and ovarian cancers. BRCA1 mutation carriers face a cumulative lifetime risk of ovarian and breast cancer development. We report on a case of a strong family prevalence of BRCA1 linked malignancies as an immense psychological encumbrance and reason of demand for radical prophylactic risk decreasing surgeries in a 29 year-old healthy woman with proved 3889delAG BRCA1 gene mutation on exon 11, codon 1265 in effort to prevent possible malignant changes in the ovaries and the breast. Problems regarding the management of asymptomatic BRCA mutation carriers, time and impact of early prophylactic surgery in young women are discussed with a review of recent literature.

P41. The role of IL-10 polymorphism in pathology of hypertensive disorders in pregnancy

P40. MIR-21 and mir-221 overexpression in placental tissue of preeclamptic patients

P42. TNF-ALPHA gene polymorphism in pathology of preeclampsia

Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health, 2015

P40. MIR-21 and mir-221 overexpression in placental tissue of preeclamptic patients

Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health, 2015

[Recurrent subareolar non puerperal abscess of breast with fistules of lactiferous ducts (Zuskas disease).]

Rozhledy v chirurgii: měsíčník Československé chirurgické společnosti

Introduction: Zuskas disease (ZD) is an illness also known as the recurrent subareolar non-puerpe... more Introduction: Zuskas disease (ZD) is an illness also known as the recurrent subareolar non-puerperal abscess of breast with fistulas of lactiferous ducts or as a periductal mastitis. ZD is rare, but painful chronic disease of breast characterized by local inflammation and evacuation of viscous content from abscess around the nipple. We present a rare form of the non-puerperal mastitis in patients with recurrence of this disease and with the description of management and treatment. Conclusion: The treatment of ZD is often inadequate and it leads to the recurrence of ZD and retraction of the nipple. Definitive treatment of ZD is surgical excision of the fistulation, removing of whole retroareolar fibroglandular tissue, abscess cavity and ductal tissue inside the nipple, including the obstructed ducts. The ratio of patients cured by this method is high, as well as their satisfaction with the final cosmetic effect of the nipple and breast. Key words: Zuskas disease - Recurrent subareola...

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[Correlation between the incidence of PIK3CA mutations in breast cancer and histopathological characteristics of the tumor.]

Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne

Objective: To determine the presence of mutations in exon 9 (encoding the helical domain) and exo... more Objective: To determine the presence of mutations in exon 9 (encoding the helical domain) and exon 20 (encoding the kinase domain) of phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha (PIK3CA) gene in DNA obtained from paraffin embedded tissue from patients with carcinoma of the mammary gland and to correlate results with clinicopathological characteristics of cancer.Design: Prospective clinical study.Setting: Department of Molecular Biology, Department of Obstetrics and Gynecology, Jessenius Faculty of Medicine, Commenius University, Martin, Slovak Republic.Methods: In set of 95 tissue samples from patients with breast cancer, mutations in exon 9 and 20 were analysed by sequencing. We also observed the associations between mutations and histopathological characteristics of tumor.Results: Overall, mutations were present in 25.3% (24/95) of PIK3CA gene, of this 14.7% (14/95) of mutations were located in exon 9 and 10.5% (10/95) of mutations were in exon 20. We d...

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OC193: The effects of epidural analgesia on the fetus: a Doppler ultrasonographic study

Ultrasound in Obstetrics and Gynecology, 2007

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[Perinatal indicators of the Zilina region in Slovak Republic during the period 2000-2009]

Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne

To provide health care providers, patients, and the general public the improvements and objective... more To provide health care providers, patients, and the general public the improvements and objective data outcomes in perinatal health care indicators for the Zilina district, northern part of Slovak republic. Martin perinatology center (Department of Gynecology and Obstetrics, Department of Neonatology, Jessenius Medical Faculty, Commenius University, Martin, Slovak Republic). Retrospective analysis of selected main perinatal outcomes for the period of 10 years (last decade) in the Zilina region. The comparison between the regional data and similar ones retrieved for the Slovak republic. During the analyzed period we have observed a significant decrease in perinatal mortality (PM) with lowest rate of 3.1 per thousand in year 2009 with the 4.13 per thousand decade average. The more favorable trend in PM drop was observed when analyzed separately from congenital disorders, which when compared to national rate decreased by 2.9 per thousand and by 1 per thousand when compared to crude PM ...

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[The p16INK4A mRNA transcripts estimation in cervical smear with different degrees of cervical dysplasia]

Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, 2012

To determine the presence of HPV infection and expression level of p16INK4A mRNA transcripts in c... more To determine the presence of HPV infection and expression level of p16INK4A mRNA transcripts in cervical smears as adjunct biomarker in detection of cervical intraepithelial neoplasia or cancer. Prospective pilot clinical study assessing clinical utility and validity of ddCt method for qPCR mRNA expression of p16ink4a in comparison to immunohistochemistry. Department of Molecular Biology, Department of Obstetrics and Gynecology, Jessenius Medical Faculty, Commenius University, Martin, Slovak Republic. Cervical smears (OC) from patients with different cervical lesions (L-SIL, H-SIL, SCA; n=45) and from healthy controls (n=45) were tested for the presence of HPV infection and p16INK4A mRNA transcripts using relative quantification (RQ). Results were compared to H&E and IHC histological findings from biopsies (conization, hysterectomy). HPV 16 was the most frequent finding (53.3%) in the group of subjects with cervical dysplasia. The p16INK4A mRNA expression analysis revealed the sligh...

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[Methylation of selected tumor-supressor genes in benign and malignant ovarian tumors]

Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, 2011

To evaluate the usefullness of examination of methylation status of selected tumor-supressor gene... more To evaluate the usefullness of examination of methylation status of selected tumor-supressor genes in early diagnosis of ovarian cancer. Prospective clinical study. Department of Gynecology and Obstetrics, Department of Molecular Biology, Jessenius Medical Faculty, Commenius University, Martin, Slovak Republic. In this study we analyzed hypermethylation of 5 genes RASSF1A, GSTP, E-cadherin, p16 and APC in ovarian tumor samples from 34 patients - 13 patients with epithelial ovarian cancer, 2 patients with border-line ovarian tumors, 12 patients with benign lesions of ovaries and 7 patients with healthy ovarian tissue. The methylation status of promoter region of tumor-supressor genes was determined by Methylation Specific Polymerase Chain Reaction (MSP) using a nested two-step approach with bisulfite modified DNA template and specific primers. Gene methylation analysis revealed hypermethylation of gene RASSF1A (46%) and GSTP (8%) only in malignant ovarian tissue samples. Ecad, p16 an...

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[Semiquantitative analysis of mRNA aromatase expression in eutopic endometrium as a diagnostic marker of endometriosis and estrogen dependent diseases]

Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, 2008

To determine clinical benefits of mRNA aromatase expression in entopic endometrium as a diagnosti... more To determine clinical benefits of mRNA aromatase expression in entopic endometrium as a diagnostic marker of endometriosis. Prospective clinical trial. Department of Obstetrics and Gynaecology of Jessenius Medical Faculty and Faculty Hospital, Martin. The expression of mRNA aromatase of eutopic endometrium was determined among women who underwent laparoscopy or laparotomy due to pelvic pain, infertility or benign pelvic tumor. Endometriosis was confirmed histologicaly and classified by rAFS. On the basis of entering criteria 23 women were enrolled in this study and divided into two subgroups: 12 endometriotic and 11 without endometriosis. Sensitivity of aromatase expression was 75% and specificity 54.5% at the cut-off value of at least minimal aromatase activity. By the presence of estrogen-dependent diseases- endometriosis, myomas or endometrial hyperplasia 18 women were compared to 5 disease free women. In this case, sensitivity of aromatase expression was 72.2 and specificity 80%...

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[Is diagnostic curettage sufficient prior to endometrial ablation? Case of an early stage endometrial cancer]

Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, 2005

To demonstrate a case of unrecognized endometrial cancer at endometrial ablation. Case report. De... more To demonstrate a case of unrecognized endometrial cancer at endometrial ablation. Case report. Department of Obstetrics and Gynaecology and Department of Pathology, Jessenius Faculty Hospital, Martin, Slovakia. Authors describe a case of an early endometrial cancer diagnosed from material obtained by bipolar loop electrode during endometrial ablation. Authors poing out insufficiency of curettage as a method of endometrial biopsy prior to ablation. Using of the loop electrode may minimize the incidence of unrecognized malignancies.

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[Acatholytic variant of squamous carcinoma of the breast. A case report and review of literature]

Ceskoslovenská patologie, 2011

The acantholytic variant of squamous carcinoma (ASC) represents a rare type of metaplastic breast... more The acantholytic variant of squamous carcinoma (ASC) represents a rare type of metaplastic breast carcinoma with typical occurrence of pseudoglandular and pseudovascular structures, arising as a result of cohesion loss between the neoplastic cells. Up to the present, there have been only 10 cases of mammary ASC described in the English written literature. The authors present a case of a 57-year-old woman with a large (6 x 7 cm) suspicious lump on ultrasonography in her right breast treated by mastectomy with an ipsilateral axillary lymph node dissection due to histologically verified ASC. Additional postoperative staging computer tomography revealed metastatic foci in the left lungs, thus calling for adjuvant chemotherapy for the patient. Six months after setting the diagnosis, the patient is alive with a partial therapeutic response. In the differential diagnosis of ASC it is important to exclude angiosarcoma, phyllodes tumor and metastatic sarcomas to the breast. The useful tools ...

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Repetitive demand for radical cancer risk reduction surgery in a young BRCA1 mutation carrier with strong family history of BRCA linked malignancies

Ginekologia polska, 2006

Gene expression profiling of histologically normal breast tissue in females with human epidermal growth factor receptorï¿½2‑positive breast cancer

Molecular Medicine Reports, 2014

Gene expression profile-based taxonomy of breast cancer (BC) has been described as a significant ... more

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C30 Detection of Methylation of GSTP1, E-Cad and PTGS2 in Prostate Adenocarcinoma and Benign Prostate Hyperplasia

European Urology Supplements, 2010

Eur Urol Suppl 2010;9(6):626 Conclusions: In 81% of patients treated by PVP I-PSS and QoL decreas... more Eur Urol Suppl 2010;9(6):626 Conclusions: In 81% of patients treated by PVP I-PSS and QoL decreased after 4 weeks. Significant increase of Qmax was observed in pts after 4 weeks from PVP. In over 90 % of pts no sings of bacteriuria were observed after treatment. In pts with prostate volume over 120 cc haematuria and incidents of urinary retention which caused another method of treatment were observed.

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