Sunscreens are known to play an important role on the prevention of skin cancer, as they act by b... more Sunscreens are known to play an important role on the prevention of skin cancer, as they act by blocking the carcinogenic solar radiations. The currently methods for determining the sun protection efficacy of these products are all based on in vivo tests. In this light, the aim of the present study was to compare the performance of two spectrophotometric in vitro methods, in order to determine which one generates more trustworthy Sun Protection Factors (SPF) values: the classical UV spectrophotometry or the diffuse transmittance reflectance spectrophotometry. For that, twenty-five SPF 30 commercial sunscreen samples were used. The methods generated different results, being the diffuse transmittance spectrophotometry more appropriate and reliable for determining the SPF of these products. Moreover, it provides as additional advantage the possibility of quantifying the protection against UVA radiation through the UVA/UVB Ratio and the Critical Wavelength.
2012 25th SIBGRAPI Conference on Graphics, Patterns and Images Tutorials, 2012
This survey provides GLSL information for beginners, by means of a series of commented codes and ... more This survey provides GLSL information for beginners, by means of a series of commented codes and technical explanations, providing an effective way for learning GLSL, one of the main multi-platform and multi-hardware shader programming languages available. The examples increase in complexity through the text. They may run in several shader tools, including Shaderlabs, a shader development environment, which was developed aiming at helping those willing to learn and practice GLSL development.
Double strand break lesions, the most toxic type of DNA damage, are repaired primarily through 2 ... more Double strand break lesions, the most toxic type of DNA damage, are repaired primarily through 2 distinct pathways: homology-directed recombination (HR) and non-homologous end-joining (NHEJ). BRCA1 and 53BP1, 2 proteins containing the BRCT modular domain, play an important role in DNA damage response (DDR) by orchestrating the decision between HR and NHEJ, but the precise mechanisms regarding both pathways are not entirely understood. Previously, our group identified a putative interaction between BRCA1 and BARD1 (BRCA1-associated RING domain 1) and the cyclin-dependent kinase (CDK9). CDK9 is a component of the positive transcription elongation complex and has been implicated in genome integrity maintenance associated with the replication stress response. Here we show that CDK9 interacts with endogenous BRCA1 and BARD1 mediated by their RING finger and BRCT domains, and describe CDK9 ionizing radiation-induced foci (IRIF) formation and its co-localization with BRCA1 in DNA damage sites. Cells lacking CDK9 are characterized by an altered γ-H2AX foci dynamics after DNA damage, a reduced efficiency in HR but not in NHEJ repair, failure to form BRCA1 and RAD51 IRIF and increased sensitivity to genotoxic agents. These data indicate that CDK9 is a player in the DDR and is consistent with its participation in HR pathway by modulating BRCA1 response.
To evaluate the destruction complex of beta-catenin by the expression of the proteins beta-catete... more To evaluate the destruction complex of beta-catenin by the expression of the proteins beta-catetenin, adenomatous polyposis coli, GSK3β, axin and ubiquitin in colorectal carcinoma and colonic adenoma. Tissue samples from 64 patients with colorectal carcinoma and 53 patients with colonic adenoma were analyzed. Tissue microarray blocks and slides were prepared and subjected to immunohistochemistry with polyclonal antibodies in carcinoma, adjacent non-neoplastic mucosa, and adenoma tissues. The immunoreactivity was evaluated by the percentage of positive stained cells and by the intensity assessed through of the stained grade of proteins in the cytoplasm and nucleus of cells. In the statistical analysis, the Spearman correlation coefficient, Student's t, χ2, Mann-Whitney, and McNemar tests, and univariate logistic regression analysis were used. In colorectal carcinoma, the expressions of beta-catenin and adenomatous polyposis coli proteins were significantly higher than in colonic ...
The Wnt family is involved in tumorigenesis of several tissues. In ovarian cancer, the role playe... more The Wnt family is involved in tumorigenesis of several tissues. In ovarian cancer, the role played by Wnts and its pathways is not clearly defined. In order to analyze the canonical and noncanonical Wnt pathway in normal ovary, benign ovarian tumor and ovarian cancer, we evaluated the immunohistochemical expression of Wnt1, Frizzled-1 (FZD1), Wnt5a, Frizzled-5 (FZD5) and ß-catenin. Ovarian specimens were obtained from surgeries performed between 1993 and 2004. The patients were divided in three groups: group A, epithelial ovarian cancer (n=38); group B, benign epithelial neoplasia (n=28); and group C, normal ovaries (n=26). Immunoreactivity for Wnt1, FZD1, Wnt5a, FZD5 and ß-catenin was scored for each group. The proportion of Wnt1 positive women in group A (29.4%) was significantly higher than in group B (4.3%) and C (9.1%) (p=0.020). The proportion of FZD1 positive patients in group C (54.5%) was significantly lower than in group A (97.1%) and B (90.0%) (p<0.001). The proportion of Wnt5a positive women was significantly higher for group A (80.0%) compared to group B (25.0%) and C (27.3%) (p<0.001). The proportion of ßcatenin positive patients in group C (95.8%) was significantly higher than group B (52.4%) (p=0.004). Comparison of the survival curves in group A according to Wnt5a expression showed a significant difference between positive and negative patients, whereas the Wnt5a positive women showed worse results (p=0.050). Our findings suggest that the pathways related to Wnt5a have an important role in ovarian malignant neoplasia. Furthermore, Wnt5a was found to be a predictor of poor prognosis for ovarian cancer.
The interaction between cellulose and xylan is important for the load-bearing secondary cell wall... more The interaction between cellulose and xylan is important for the load-bearing secondary cell wall of flowering plants. Based on the precise even-spaced pattern of acetyl and glucuronosyl (MeGlcA) xylan substitutions in eudicots, we recently proposed that an unsubstituted face of xylan in a two-fold helical screw can hydrogen bond to the hydrophilic surfaces of cellulose microfibrils. In gymnosperm cell walls, any role for xylan is unclear, and glucomannan is thought to be the important cellulose-binding polysaccharide. Here, we analysed xylan from the secondary cell walls of the four gymnosperm lineages (Conifer, Gingko, Cycad and Gnetophyta). Conifer, Gingko and Cycad xylan lacks acetylation, but is modified by arabinose and MeGlcA. Interestingly, the arabinosyl substitutions are located two xylosyl residues from MeGlcA, which is itself placed precisely on every sixth xylosyl residue. Notably, the Gnetophyta xylan is more akin to early-branching angiosperms and eudicot xylan, lacki...
Revista Brasileira De Prescricao E Fisiologia Do Exercicio, Aug 15, 2010
ISSN 1981-9900 versão eletrônica P e r i ó d i c o do I n s t i t u t o B r a s i l e i r o d e P... more ISSN 1981-9900 versão eletrônica P e r i ó d i c o do I n s t i t u t o B r a s i l e i r o d e P e sq u i s a e E n si n o e m F i s i o l o gi a do E x e r c í c i o w w w . i b p e f e x . c o m . b r / w w w . r b p f e x . c o m . b r ISSN 1981-9900 versão eletrônica P e r i ó d i c o do I n s t i t u t o B r a s i l e i r o d e P e sq u i s a e E n si n o e m F i s i o l o gi a do E x e r c í c i o w w w . i b p e f e x . c o m . b r / w w w . r b p f e x . c o m . b r
The Journal of Strength and Conditioning Research, Dec 18, 2012
Improving the process of how physical performance is enhanced is one of the main topics evaluated... more Improving the process of how physical performance is enhanced is one of the main topics evaluated by physiologists. This process often involves athletes and nonathletic populations. The purpose of this study was to assess the chronic response to 10 weeks of static stretching exercises carried out before and during a strength training program for 8 exercises on an 8 repetition maximum (8RM) test performance, and basal serum insulinlike growth factor (IGF-1) levels. Thirty recreationally trained volunteers were randomly assigned to 1 of 3 training groups: (a) SBST (performed a warm-up with a static stretching protocol before each strength training session); (b) SDST (before each training set, a static stretching exercise was performed); and (c) OST (entire session was performed without any type of stretching exercise). Strength and IGF-1 levels were collected at the beginning (pretest) and end (posttest) of the entire experimental procedure. All the exercises showed a significant increase in muscle strength for the OST group. However, the results revealed a significant increase in the muscle strength for only a few exercises in the SBST (LP, LE) and SDST (LP) experimental conditions. Significant statistical differences were found between SBST and SDST for all the exercises in the OST experimental condition. Furthermore, the IGF-1 expression showed no significant differences in the intragroup analysis. However, the OST group showed higher values (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05) in the posttest when compared with those of the other groups (increased significantly only in the OST experimental condition). It has been concluded that, although all the groups showed an increase in muscular strength, the strength training performed without any type of stretching exercise, regardless of whether the stretching is performed before or during the lifting session, can more effectively increase muscle strength and basal serum IGF-1 levels. It was concluded that strength training, with or without the use of stretching exercises, increased muscular strength in the studied groups, and can induce an increase in IGF-1 levels.
Sao Paulo Medical Journal Revista Paulista De Medicina, 2011
CONTEXT AND OBJECTIVE: The Wnt pathway is involved in tumorigenesis of several tissues. For this ... more CONTEXT AND OBJECTIVE: The Wnt pathway is involved in tumorigenesis of several tissues. For this reason, we proposed to evaluate Wnt gene expression in endometrial cancer type I. DESIGN AND SETTING: Cross-sectional study on materials gathered from the tissue bank of the Department of Pathology, Universidade Federal de São Paulo. METHODS: Endometrial specimens were obtained from surgeries performed between 1995 and 2005 at São Paulo Hospital, Universidade Federal de São Paulo. The material was divided into two groups according to tissue type: Group A, atrophic endometrium (n = 15); and Group B, endometrial adenocarcinoma (n = 45). We compared the immunohistochemical expression of Wnt1, Frizzled-1 (FZD1), Wnt5a, Frizzled-5 (FZD5) and beta-catenin between endometrial cancer type I and atrophic endometrium. RESULTS: Regarding Wnt1, FZD1 and Wnt5a expression, no significant association was observed between the groups. A significant association was observed between the groups in relation to FZD5 expression (P = 0.001). The proportion of FZD5-positive samples was significantly higher in group A (80.0%) than in group B (31.1%). Regarding the survival curve for FZD5 in group B, we did not find any significant association between atrophic endometrium and endometrial adenocarcinoma. We also did not find any significant association regarding beta-catenin expression (P = 1.000). CONCLUSION: FZD5 is downregulated in endometrial adenocarcinoma, in comparison with atrophic endometrium.
Page 1. Acute effect of passive rest intervals and stretching exercise on multiple set performanc... more Page 1. Acute effect of passive rest intervals and stretching exercise on multiple set performance Antonio Claudio do Rosário Souza, Carmen Lúcia Borges Bastos, Maria de Nazaré Dias Portal, Belmiro Freitas de Salles, Thiago Matassoli Gomes, Jefferson da Silva Novaes ...
The aim of this study was to analyze the immunoexpression of (Smac) DIABLO, AIF, cytochrome c, Ki... more The aim of this study was to analyze the immunoexpression of (Smac) DIABLO, AIF, cytochrome c, Ki-67 and cleaved caspase-3 in gastric cancer. A tissue microarray (TMA) paraffin block was constructed using gastric adenocarcinoma tissue and adjacent normal adjacent mucosa from 87 patients who had not previously undergone radiotherapy or chemotherapy. Immunohistochemistry was used to evaluate the protein levels. Samples were positive for (Smac) DIABLO in 37 (45.6%) and 37 (46.8%), for AIF in 31 (36.9%) and 36 (45.6%), for cytochrome c in 60 (68.9%) and 44 (54.4%), for Ki-67 in 63 (72.4%) and 52 (61.9%) and for cleaved caspase-3 in 21 (24.1%) and 3 (3.4%) cases of tumor and adjacent normal tissues, respectively. Our results suggest that increased expression of Ki-67 and cleaved caspase-3 could contribute to carcinogenesis. The expression of these proteins indicates an attempt of cells to maintain tissue homeostasis.
Italian Journal of Anatomy and Embryology Archivio Italiano Di Anatomia Ed Embriologia, 2008
The objective of this paper was to study the morphology of the articular disc and analyze the imm... more The objective of this paper was to study the morphology of the articular disc and analyze the immunohistochemical expression of the marker of type IV collagen in the articular disc of the temporomandibular joint (TMJ) of human fetuses of different gestational ages. Twenty TMJ from human fetuses aging from 21 to 24 weeks of intrauterine life were studied. The TMJ were supplied by the Federal University of Uberaba. The ages of the fetuses were determined by measuring the crown-rump length (CRL). Macroscopically, the fetuses were fixed in a formalin solution at 10% and dissected by removing the skin and the subcutaneous tissue, exposing the deep structures. An immunohistochemical marker of type IV collagen was used in order to characterize the presence of blood vessels in the central region of the temporomandibular joint disc. Analysis of the immunohistochemical marker of type IV collagen showed the presence of blood vessels in the central region of the temporomandibular disc in human fetuses.
Sunscreens are known to play an important role on the prevention of skin cancer, as they act by b... more Sunscreens are known to play an important role on the prevention of skin cancer, as they act by blocking the carcinogenic solar radiations. The currently methods for determining the sun protection efficacy of these products are all based on in vivo tests. In this light, the aim of the present study was to compare the performance of two spectrophotometric in vitro methods, in order to determine which one generates more trustworthy Sun Protection Factors (SPF) values: the classical UV spectrophotometry or the diffuse transmittance reflectance spectrophotometry. For that, twenty-five SPF 30 commercial sunscreen samples were used. The methods generated different results, being the diffuse transmittance spectrophotometry more appropriate and reliable for determining the SPF of these products. Moreover, it provides as additional advantage the possibility of quantifying the protection against UVA radiation through the UVA/UVB Ratio and the Critical Wavelength.
2012 25th SIBGRAPI Conference on Graphics, Patterns and Images Tutorials, 2012
This survey provides GLSL information for beginners, by means of a series of commented codes and ... more This survey provides GLSL information for beginners, by means of a series of commented codes and technical explanations, providing an effective way for learning GLSL, one of the main multi-platform and multi-hardware shader programming languages available. The examples increase in complexity through the text. They may run in several shader tools, including Shaderlabs, a shader development environment, which was developed aiming at helping those willing to learn and practice GLSL development.
Double strand break lesions, the most toxic type of DNA damage, are repaired primarily through 2 ... more Double strand break lesions, the most toxic type of DNA damage, are repaired primarily through 2 distinct pathways: homology-directed recombination (HR) and non-homologous end-joining (NHEJ). BRCA1 and 53BP1, 2 proteins containing the BRCT modular domain, play an important role in DNA damage response (DDR) by orchestrating the decision between HR and NHEJ, but the precise mechanisms regarding both pathways are not entirely understood. Previously, our group identified a putative interaction between BRCA1 and BARD1 (BRCA1-associated RING domain 1) and the cyclin-dependent kinase (CDK9). CDK9 is a component of the positive transcription elongation complex and has been implicated in genome integrity maintenance associated with the replication stress response. Here we show that CDK9 interacts with endogenous BRCA1 and BARD1 mediated by their RING finger and BRCT domains, and describe CDK9 ionizing radiation-induced foci (IRIF) formation and its co-localization with BRCA1 in DNA damage sites. Cells lacking CDK9 are characterized by an altered γ-H2AX foci dynamics after DNA damage, a reduced efficiency in HR but not in NHEJ repair, failure to form BRCA1 and RAD51 IRIF and increased sensitivity to genotoxic agents. These data indicate that CDK9 is a player in the DDR and is consistent with its participation in HR pathway by modulating BRCA1 response.
To evaluate the destruction complex of beta-catenin by the expression of the proteins beta-catete... more To evaluate the destruction complex of beta-catenin by the expression of the proteins beta-catetenin, adenomatous polyposis coli, GSK3β, axin and ubiquitin in colorectal carcinoma and colonic adenoma. Tissue samples from 64 patients with colorectal carcinoma and 53 patients with colonic adenoma were analyzed. Tissue microarray blocks and slides were prepared and subjected to immunohistochemistry with polyclonal antibodies in carcinoma, adjacent non-neoplastic mucosa, and adenoma tissues. The immunoreactivity was evaluated by the percentage of positive stained cells and by the intensity assessed through of the stained grade of proteins in the cytoplasm and nucleus of cells. In the statistical analysis, the Spearman correlation coefficient, Student's t, χ2, Mann-Whitney, and McNemar tests, and univariate logistic regression analysis were used. In colorectal carcinoma, the expressions of beta-catenin and adenomatous polyposis coli proteins were significantly higher than in colonic ...
The Wnt family is involved in tumorigenesis of several tissues. In ovarian cancer, the role playe... more The Wnt family is involved in tumorigenesis of several tissues. In ovarian cancer, the role played by Wnts and its pathways is not clearly defined. In order to analyze the canonical and noncanonical Wnt pathway in normal ovary, benign ovarian tumor and ovarian cancer, we evaluated the immunohistochemical expression of Wnt1, Frizzled-1 (FZD1), Wnt5a, Frizzled-5 (FZD5) and ß-catenin. Ovarian specimens were obtained from surgeries performed between 1993 and 2004. The patients were divided in three groups: group A, epithelial ovarian cancer (n=38); group B, benign epithelial neoplasia (n=28); and group C, normal ovaries (n=26). Immunoreactivity for Wnt1, FZD1, Wnt5a, FZD5 and ß-catenin was scored for each group. The proportion of Wnt1 positive women in group A (29.4%) was significantly higher than in group B (4.3%) and C (9.1%) (p=0.020). The proportion of FZD1 positive patients in group C (54.5%) was significantly lower than in group A (97.1%) and B (90.0%) (p<0.001). The proportion of Wnt5a positive women was significantly higher for group A (80.0%) compared to group B (25.0%) and C (27.3%) (p<0.001). The proportion of ßcatenin positive patients in group C (95.8%) was significantly higher than group B (52.4%) (p=0.004). Comparison of the survival curves in group A according to Wnt5a expression showed a significant difference between positive and negative patients, whereas the Wnt5a positive women showed worse results (p=0.050). Our findings suggest that the pathways related to Wnt5a have an important role in ovarian malignant neoplasia. Furthermore, Wnt5a was found to be a predictor of poor prognosis for ovarian cancer.
The interaction between cellulose and xylan is important for the load-bearing secondary cell wall... more The interaction between cellulose and xylan is important for the load-bearing secondary cell wall of flowering plants. Based on the precise even-spaced pattern of acetyl and glucuronosyl (MeGlcA) xylan substitutions in eudicots, we recently proposed that an unsubstituted face of xylan in a two-fold helical screw can hydrogen bond to the hydrophilic surfaces of cellulose microfibrils. In gymnosperm cell walls, any role for xylan is unclear, and glucomannan is thought to be the important cellulose-binding polysaccharide. Here, we analysed xylan from the secondary cell walls of the four gymnosperm lineages (Conifer, Gingko, Cycad and Gnetophyta). Conifer, Gingko and Cycad xylan lacks acetylation, but is modified by arabinose and MeGlcA. Interestingly, the arabinosyl substitutions are located two xylosyl residues from MeGlcA, which is itself placed precisely on every sixth xylosyl residue. Notably, the Gnetophyta xylan is more akin to early-branching angiosperms and eudicot xylan, lacki...
Revista Brasileira De Prescricao E Fisiologia Do Exercicio, Aug 15, 2010
ISSN 1981-9900 versão eletrônica P e r i ó d i c o do I n s t i t u t o B r a s i l e i r o d e P... more ISSN 1981-9900 versão eletrônica P e r i ó d i c o do I n s t i t u t o B r a s i l e i r o d e P e sq u i s a e E n si n o e m F i s i o l o gi a do E x e r c í c i o w w w . i b p e f e x . c o m . b r / w w w . r b p f e x . c o m . b r ISSN 1981-9900 versão eletrônica P e r i ó d i c o do I n s t i t u t o B r a s i l e i r o d e P e sq u i s a e E n si n o e m F i s i o l o gi a do E x e r c í c i o w w w . i b p e f e x . c o m . b r / w w w . r b p f e x . c o m . b r
The Journal of Strength and Conditioning Research, Dec 18, 2012
Improving the process of how physical performance is enhanced is one of the main topics evaluated... more Improving the process of how physical performance is enhanced is one of the main topics evaluated by physiologists. This process often involves athletes and nonathletic populations. The purpose of this study was to assess the chronic response to 10 weeks of static stretching exercises carried out before and during a strength training program for 8 exercises on an 8 repetition maximum (8RM) test performance, and basal serum insulinlike growth factor (IGF-1) levels. Thirty recreationally trained volunteers were randomly assigned to 1 of 3 training groups: (a) SBST (performed a warm-up with a static stretching protocol before each strength training session); (b) SDST (before each training set, a static stretching exercise was performed); and (c) OST (entire session was performed without any type of stretching exercise). Strength and IGF-1 levels were collected at the beginning (pretest) and end (posttest) of the entire experimental procedure. All the exercises showed a significant increase in muscle strength for the OST group. However, the results revealed a significant increase in the muscle strength for only a few exercises in the SBST (LP, LE) and SDST (LP) experimental conditions. Significant statistical differences were found between SBST and SDST for all the exercises in the OST experimental condition. Furthermore, the IGF-1 expression showed no significant differences in the intragroup analysis. However, the OST group showed higher values (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.05) in the posttest when compared with those of the other groups (increased significantly only in the OST experimental condition). It has been concluded that, although all the groups showed an increase in muscular strength, the strength training performed without any type of stretching exercise, regardless of whether the stretching is performed before or during the lifting session, can more effectively increase muscle strength and basal serum IGF-1 levels. It was concluded that strength training, with or without the use of stretching exercises, increased muscular strength in the studied groups, and can induce an increase in IGF-1 levels.
Sao Paulo Medical Journal Revista Paulista De Medicina, 2011
CONTEXT AND OBJECTIVE: The Wnt pathway is involved in tumorigenesis of several tissues. For this ... more CONTEXT AND OBJECTIVE: The Wnt pathway is involved in tumorigenesis of several tissues. For this reason, we proposed to evaluate Wnt gene expression in endometrial cancer type I. DESIGN AND SETTING: Cross-sectional study on materials gathered from the tissue bank of the Department of Pathology, Universidade Federal de São Paulo. METHODS: Endometrial specimens were obtained from surgeries performed between 1995 and 2005 at São Paulo Hospital, Universidade Federal de São Paulo. The material was divided into two groups according to tissue type: Group A, atrophic endometrium (n = 15); and Group B, endometrial adenocarcinoma (n = 45). We compared the immunohistochemical expression of Wnt1, Frizzled-1 (FZD1), Wnt5a, Frizzled-5 (FZD5) and beta-catenin between endometrial cancer type I and atrophic endometrium. RESULTS: Regarding Wnt1, FZD1 and Wnt5a expression, no significant association was observed between the groups. A significant association was observed between the groups in relation to FZD5 expression (P = 0.001). The proportion of FZD5-positive samples was significantly higher in group A (80.0%) than in group B (31.1%). Regarding the survival curve for FZD5 in group B, we did not find any significant association between atrophic endometrium and endometrial adenocarcinoma. We also did not find any significant association regarding beta-catenin expression (P = 1.000). CONCLUSION: FZD5 is downregulated in endometrial adenocarcinoma, in comparison with atrophic endometrium.
Page 1. Acute effect of passive rest intervals and stretching exercise on multiple set performanc... more Page 1. Acute effect of passive rest intervals and stretching exercise on multiple set performance Antonio Claudio do Rosário Souza, Carmen Lúcia Borges Bastos, Maria de Nazaré Dias Portal, Belmiro Freitas de Salles, Thiago Matassoli Gomes, Jefferson da Silva Novaes ...
The aim of this study was to analyze the immunoexpression of (Smac) DIABLO, AIF, cytochrome c, Ki... more The aim of this study was to analyze the immunoexpression of (Smac) DIABLO, AIF, cytochrome c, Ki-67 and cleaved caspase-3 in gastric cancer. A tissue microarray (TMA) paraffin block was constructed using gastric adenocarcinoma tissue and adjacent normal adjacent mucosa from 87 patients who had not previously undergone radiotherapy or chemotherapy. Immunohistochemistry was used to evaluate the protein levels. Samples were positive for (Smac) DIABLO in 37 (45.6%) and 37 (46.8%), for AIF in 31 (36.9%) and 36 (45.6%), for cytochrome c in 60 (68.9%) and 44 (54.4%), for Ki-67 in 63 (72.4%) and 52 (61.9%) and for cleaved caspase-3 in 21 (24.1%) and 3 (3.4%) cases of tumor and adjacent normal tissues, respectively. Our results suggest that increased expression of Ki-67 and cleaved caspase-3 could contribute to carcinogenesis. The expression of these proteins indicates an attempt of cells to maintain tissue homeostasis.
Italian Journal of Anatomy and Embryology Archivio Italiano Di Anatomia Ed Embriologia, 2008
The objective of this paper was to study the morphology of the articular disc and analyze the imm... more The objective of this paper was to study the morphology of the articular disc and analyze the immunohistochemical expression of the marker of type IV collagen in the articular disc of the temporomandibular joint (TMJ) of human fetuses of different gestational ages. Twenty TMJ from human fetuses aging from 21 to 24 weeks of intrauterine life were studied. The TMJ were supplied by the Federal University of Uberaba. The ages of the fetuses were determined by measuring the crown-rump length (CRL). Macroscopically, the fetuses were fixed in a formalin solution at 10% and dissected by removing the skin and the subcutaneous tissue, exposing the deep structures. An immunohistochemical marker of type IV collagen was used in order to characterize the presence of blood vessels in the central region of the temporomandibular joint disc. Analysis of the immunohistochemical marker of type IV collagen showed the presence of blood vessels in the central region of the temporomandibular disc in human fetuses.
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