Proteases are one of the most important classes of industrial enzymes. They execute a large varie... more Proteases are one of the most important classes of industrial enzymes. They execute a large variety of functions and have important biotechnological applications. Proteases represent one of the three largest groups of industrial enzymes and find application in detergents, leather industry, food industry, pharmaceutical industry and bioremediation processes and accounts for about 60% of commercial enzymes in the world. These enzymes are found in a wide diversity of sources such as plants, animals and microorganisms but they are mainly produced by bacteria and fungi. Fungi elaborate a wide variety of proteolytic enzymes than bacteria. Under this study, isolation of fungal species from deteriorating wall paintings of caves and rocks of Sarguja district, Ambikapur (Chhattisgarh, India) was done followed by its identification. Preliminary screening for protease production was done by incorporating 1% casein/ gelatin in Asthana and Hawker's medium and A. fumigatus gave positive result. Highest protease production by A. fumigatus was recorded at pH 5.5 followed by pH 6.0, but gradually declined beyond these values and increase in final pH was observed. Enzyme production by test fungi varies significantly with increase and decrease in temperature and maximum yield was observed at temperature 32 ± 2ºC. Carbon sources such as maltose, cellulose, sorbitol and mannitol enhanced the growth of enzyme whereas addition of inorganic nitrogen sources suppressed enzyme production except urea and sodium nitrate which stimulate extracellular protease liberation in the medium at lower concentration while at higher concentration enzyme production declined significantly.
Proteases are one of the most important classes of industrial enzymes. They execute a large varie... more Proteases are one of the most important classes of industrial enzymes. They execute a large variety of functions and have important biotechnological applications. Proteases represent one of the three largest groups of industrial enzymes and find application in detergents, leather industry, food industry, pharmaceutical industry and bioremediation processes and accounts for about 60% of commercial enzymes in the world. These enzymes are found in a wide diversity of sources such as plants, animals and microorganisms but they are mainly produced by bacteria and fungi. Fungi elaborate a wide variety of proteolytic enzymes than bacteria. Under this study, isolation of fungal species from deteriorating wall paintings of caves and rocks of Sarguja district, Ambikapur (Chhattisgarh, India) was done followed by its identification. Preliminary screening for protease production was done by incorporating 1% casein/ gelatin in Asthana and Hawker's medium and A. fumigatus gave positive result. Highest protease production by A. fumigatus was recorded at pH 5.5 followed by pH 6.0, but gradually declined beyond these values and increase in final pH was observed. Enzyme production by test fungi varies significantly with increase and decrease in temperature and maximum yield was observed at temperature 32 ± 2ºC. Carbon sources such as maltose, cellulose, sorbitol and mannitol enhanced the growth of enzyme whereas addition of inorganic nitrogen sources suppressed enzyme production except urea and sodium nitrate which stimulate extracellular protease liberation in the medium at lower concentration while at higher concentration enzyme production declined significantly.
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