Cordyceps militaris is one of the well described mushrooms and has been extensively used in
Tradi... more Cordyceps militaris is one of the well described mushrooms and has been extensively used in Traditional Chinese Complementary Medicine (TCCM) since many centuries. It has been cultivated naturally or in submerged culture using different types of media. Fungal mycelia contain adenosine, cordycepin, and polysaccharides, which are responsible for its biological activities. Cordycepin is the best-known and most potent mushroom-derived substances possessing anticancer, antitumor and immunomodulating activities. This mushroom characterized by very low growth rate when grown in solid agar medium for inoculum preparation during cultivation process. Thus, there was enormous need to increase the growth rate of this type of mushroom on solid medium to reduce the time of inoculum preparation stage. In this study, optimization of agar cultivation medium for rapid cell growth using modified potato dextrose agar (PDA) medium culture supplemented with specific amount of malt extract (ME) together with yeast extract (YE) was investigated. The mycelial growth diameter was monitored during 21 days of cultivation using two series of experiments of different medium supplements: 2, 4, 6 and 8 g of ME and 6 g of ME with 0, 2, 4 and 6 g of YE to the PDA medium. The obtained results clearly demonstrate that the highest mycelial growth diameter of about 7.5 cm was obtained in PDA medium supplemented with 6 g/L ME and 4 g/L. YE.
Cordyceps militaris is one of the well described mushrooms and has been extensively used in
Tradi... more Cordyceps militaris is one of the well described mushrooms and has been extensively used in Traditional Chinese Complementary Medicine (TCCM) since many centuries. It has been cultivated naturally or in submerged culture using different types of media. Fungal mycelia contain adenosine, cordycepin, and polysaccharides, which are responsible for its biological activities. Cordycepin is the best-known and most potent mushroom-derived substances possessing anticancer, antitumor and immunomodulating activities. This mushroom characterized by very low growth rate when grown in solid agar medium for inoculum preparation during cultivation process. Thus, there was enormous need to increase the growth rate of this type of mushroom on solid medium to reduce the time of inoculum preparation stage. In this study, optimization of agar cultivation medium for rapid cell growth using modified potato dextrose agar (PDA) medium culture supplemented with specific amount of malt extract (ME) together with yeast extract (YE) was investigated. The mycelial growth diameter was monitored during 21 days of cultivation using two series of experiments of different medium supplements: 2, 4, 6 and 8 g of ME and 6 g of ME with 0, 2, 4 and 6 g of YE to the PDA medium. The obtained results clearly demonstrate that the highest mycelial growth diameter of about 7.5 cm was obtained in PDA medium supplemented with 6 g/L ME and 4 g/L. YE.
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Traditional Chinese Complementary Medicine (TCCM) since many centuries. It has been cultivated naturally or
in submerged culture using different types of media. Fungal mycelia contain adenosine, cordycepin, and
polysaccharides, which are responsible for its biological activities. Cordycepin is the best-known and most
potent mushroom-derived substances possessing anticancer, antitumor and immunomodulating activities. This
mushroom characterized by very low growth rate when grown in solid agar medium for inoculum preparation
during cultivation process. Thus, there was enormous need to increase the growth rate of this type of mushroom
on solid medium to reduce the time of inoculum preparation stage. In this study, optimization of agar cultivation
medium for rapid cell growth using modified potato dextrose agar (PDA) medium culture supplemented with
specific amount of malt extract (ME) together with yeast extract (YE) was investigated. The mycelial growth
diameter was monitored during 21 days of cultivation using two series of experiments of different medium
supplements: 2, 4, 6 and 8 g of ME and 6 g of ME with 0, 2, 4 and 6 g of YE to the PDA medium. The obtained
results clearly demonstrate that the highest mycelial growth diameter of about 7.5 cm was obtained in PDA
medium supplemented with 6 g/L ME and 4 g/L. YE.
Traditional Chinese Complementary Medicine (TCCM) since many centuries. It has been cultivated naturally or
in submerged culture using different types of media. Fungal mycelia contain adenosine, cordycepin, and
polysaccharides, which are responsible for its biological activities. Cordycepin is the best-known and most
potent mushroom-derived substances possessing anticancer, antitumor and immunomodulating activities. This
mushroom characterized by very low growth rate when grown in solid agar medium for inoculum preparation
during cultivation process. Thus, there was enormous need to increase the growth rate of this type of mushroom
on solid medium to reduce the time of inoculum preparation stage. In this study, optimization of agar cultivation
medium for rapid cell growth using modified potato dextrose agar (PDA) medium culture supplemented with
specific amount of malt extract (ME) together with yeast extract (YE) was investigated. The mycelial growth
diameter was monitored during 21 days of cultivation using two series of experiments of different medium
supplements: 2, 4, 6 and 8 g of ME and 6 g of ME with 0, 2, 4 and 6 g of YE to the PDA medium. The obtained
results clearly demonstrate that the highest mycelial growth diameter of about 7.5 cm was obtained in PDA
medium supplemented with 6 g/L ME and 4 g/L. YE.