Papers by nisakorn saewan
Advanced Materials Research, Dec 1, 2012
Silver nanoparticles were prepared by treating a solution of AgNO3 dissolved in deionized water w... more Silver nanoparticles were prepared by treating a solution of AgNO3 dissolved in deionized water with Emblica (Phyllanthus emblica) extract as reducing agent. The factors that affect the synthesis of silver nanoparticles (time, temperature and concentration of silver nitrate and reducing agent) were investigated. The high concentration of silver nitrate and reducing agent showed the higher in concentration of silver nanoparticles. The heating temperature help increase the yield of silver nanoparticles at shorter reaction time. Their structure, shape and size distribution were characterized by UV-vis spectrophotometer, SEM and TEM technique. The UV-vis spectrophotometer show peaks located of silver-Emblica nanoparticle at 430 nm and the particle size of silver-Emblica is 41.2 nm in average diameter determined by TEM.
Journal of Medicinal Plants Research, Mar 18, 2011
Fractionation of an ethylacetate extract from leaves of Blumea balsamifera DC, led to isolation o... more Fractionation of an ethylacetate extract from leaves of Blumea balsamifera DC, led to isolation of nine flavonoids. The isolated compounds consisted of two dihydroflavonols, dihydroquercetin-4′ ′ ′ ′-methyl ether (1) and dihydroquercetin-7,4′ ′ ′ ′-dimethyl ether (2), two flavanones, 5,7,3′ ′ ′ ′,5′ ′ ′ ′-tetrahydroxyflavanone (3) and blumeatin (4), three flavonols, quercetin (5), rhamnetin (6) and tamarixetin (7), two flavones, luteolin (8) and luteolin-7-methyl ether (9). Their chemical structures were elucidated by spectroscopic methods including UV, NMR and MS analyses. Their inhibitory activities on mushroom tyrosinase using L-DOPA as substrate were evaluated. The anti-tyrosinase activities of dihydroflavonols (1 and 2) and flavonols (5-7) are stronger than arbutin, whereas flavanones (3 and 4) and flavones (8 and 9) are weaker than arbutin. The kinetic analysis showed that the dihydroflavonols (1 and 2), flavanones (3), and flavonols (5 and 6) are competitive inhibitors, whereas the flavones (8 and 9) are noncompetitive inhibitors. The inhibition constant (K I) of compounds 1-3 were determined to be 0.10, 0.08, and 0.33 mM, respectively. Some compounds (1-5 and 9) were evaluated for cytotoxicity against KB, MCF-7 and NCI-H187 cancer cell lines. Compounds 2, 4, and 9 were active against the KB cells with the IC 50 values of 17.09, 47.72 and 17.83 ug/ml, respectively. Compounds 2, 3 and 5 exhibited moderate activity against the NCI-H187 cells with the IC 50 values of 16.29, 29.97 and 20.59 ug/ml. Luteolin-7-methyl ether (9) showed strong cytotoxicity against human lung cancer (NCI-H187) cell lines with IC 50 of 1.29 µg/ml and moderate toxicity against oral cavity cancer (KB) cell lines with IC 50 of 17.83 µg/ml.
Acta Crystallographica Section E-structure Reports Online, Apr 22, 2011
The molecule of the title chalcone derivative, C 19 H 15 NO, exists in a trans configuration with... more The molecule of the title chalcone derivative, C 19 H 15 NO, exists in a trans configuration with respect to the C C double bond. The molecule is slightly twisted with a dihedral angle of 6.12 (12) between the benzene ring and the naphthalene ring system. The prop-2-en-1-one bridge is nearly planar, with an r.m.s. deviation of 0.0194 (2), and makes dihedral angles of 8.05 (19) and 11.47 (18) with the benzene ring and the naphthalene ring system, respectively. In the crystal, molecules are linked by N-HÁ Á ÁO hydrogen bonds into chains along the b axis. Weak N-HÁ Á Á and C-HÁ Á Á interactions and a short NÁ Á ÁO contact [2.974 (4) Å ] are also observed. organic compounds o1204 Kobkeatthawin et al.
Advanced Materials Research, Apr 1, 2012
Pulverized rhizome of Curcuma peliolata on hydrodistillation, afforded light yellow essential oil... more Pulverized rhizome of Curcuma peliolata on hydrodistillation, afforded light yellow essential oil in the yield of 0.13% v/w. The chemical constituent of the essential oil was determined by GC-MS. Nine compounds representing 95.34 % of the total oil were identified. The major compounds of the oil were 2-methyl-5-pentanol, 1H-pyrrol-1-amine,2-(4-methoxyphenyl)-n,n,5-trimethyl, and curcumol. The distillated aqueous was extracted with dicloromethane (DCM), ethyl acetate (EtOAc), and n-butanol. The DPPH radical scavenging and reducing power of the extracts were investigated. Curcumin and ascorbic acid were used as standard references. The DPPH radical scavenging activity was decrease in order: curcumin ascorbic acid EtOAc extract &gt; DCM extract &gt; n-butanol extract &gt; aqueous extract. The EtOAc extract showed significantly highest inhibition of the radical scavenging activity with IC50 0.092 ± 0.001 mg/mL (curcumin and ascorbic acid (IC50 0.012 and 0.013 mg/mL, respectively (P&lt;0.05)). While, the DCM extract showed highest reducing power with IC50 = 0.271 mg/mL which lower than that of standard curcumin (IC50 = 0.013 mg/mL) and ascorbic acid (IC50 = 0.002 mg/mL).Introduction
An entry from the Cambridge Structural Database, the world's repository for small molecule cr... more An entry from the Cambridge Structural Database, the world's repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.
Thai Journal of Agricultural Science, 2011
Cosmetics
Traditional pumpkin (Cucurbita moschata) and Japanese pumpkin (C. maxima) consist of natural poly... more Traditional pumpkin (Cucurbita moschata) and Japanese pumpkin (C. maxima) consist of natural polysaccharides. From a scientific basis, natural polysaccharides could be applied to improve hydration in the cosmetic field. The purified polysaccharide was extracted and deproteinized with the CaCl2 method. Japanese pumpkin showed the higher value of physicochemical properties including yield (12.96 ± 0.60%), total polysaccharide content (0.89 ± 0.04 mg/mL), swelling capacity (4.00 ± 0.00%), swelling index (1.04 ± 0.00%), solubility (126.67 ± 5.77%), viscosity (1.25 ± 0.00 cps), water capacity (0.93 ± 0.15 g/g) and oil absorption capacity (5.93 ± 0.06 g/g) than traditional pumpkin. Additionally, Japanese pumpkin (IC50 9.30 ± 0.58 µg/mL) provided higher antioxidant activity by DPPH assay than traditional pumpkin (IC50 9.98 ± 0.25 µg/mL). The evaluation of efficacy on skin hydration in fifteen Thai volunteers indicated that Japanese pumpkin showed non-skin irritation. An extract concentrati...
Acta Crystallographica Section E Structure Reports Online, 2004
ABSTRACT The title compound, 4-(3-furyl)-1,4,4a,5,6,6a,7,8alpha,9,10,11alpha,12-dodecahydro-4a,7,... more ABSTRACT The title compound, 4-(3-furyl)-1,4,4a,5,6,6a,7,8alpha,9,10,11alpha,12-dodecahydro-4a,7,9,9-tetramethyl-2,10,13-trioxo-7,11-meth-ano-2H-cycloocta[f][2]benzopyran-8-hydroxyacetic acid methyl ester (C27H32O8), has for the first time been isolated from Lansium domesticum, Correa. The six-membered rings adopt boat, chair, distorted sofa and half-chair conformations. The furan ring is planar. The furan substituent and hydroxyacetate group are attached equatorially to the swietenine skeleton. The crystal structure is stabilized by C-H...O Interactions.
Cosmetics, Jul 30, 2022
This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Cosmetics
The aging process encompasses gradual and continuous changes at the cellular level that slowly ac... more The aging process encompasses gradual and continuous changes at the cellular level that slowly accumulate with age. The signs of aging include many physiological changes in both skin and hair such as fine lines, wrinkles, age spots, hair thinning and hair loss. The aim of the current study was to investigate the anti-aging potential of coffee berry extract (CBE) on human dermal fibroblast (HDF) and hair follicle dermal papilla (HFDP) cells. Coffee berry was extracted by 50% ethanol and determined for chemical constituents by HPLC technique. Cytotoxicity of the extract was examined on both cells by MTT assay. Then, HDF cells were used to evaluate antioxidant properties by using superoxide dismutase activity (SOD) and nitric oxide inhibition as well as anti-collagenase inhibition assays. The effectiveness of anti-hair loss properties was investigated in HFDP cells by considering cell proliferation, 5α-reductase inhibition (5AR), and growth factor expression. The results showed that ca...
Thesis (M.Sc., Organic Chemistry)--Prince of Songkla University, 200
Molecules
Ferulic acid (FA) provides broad biological functions that have been used in cosmetics formulatio... more Ferulic acid (FA) provides broad biological functions that have been used in cosmetics formulation as a photoprotection, anti-aging, and brightening agent. However, its application is limited by its tendency to deteriorate by exposure to heat, humidity, and light. This study aimed to enhance the stability of FA by encapsulation in phosphorylated rice starch (PRS) and evaluate its effect on improving human skin. First, FA was encapsulated in PRS and characterized by FTIR, SEM, XRD, and DSC. Then, its stability when exposed to a temperature of 45 °C and light and its anti-aging effect on 16 volunteers were investigated. The results indicated that FA was successfully encapsulated in PRS with an encapsulation yield of 77%, EE (73%) and LE (65%). After 1 month at the high temperature/80%RH, the encapsulated FA retained its quantity (70%), whereas free FA was retained at only 50%. Under light exposure conditions, the encapsulated FA was retained at 65%, which was higher than FA (35%). Fra...
This study aimed to compare the bioactive compounds and their antioxidant and antimicrobial activ... more This study aimed to compare the bioactive compounds and their antioxidant and antimicrobial activities between longan (Dimocarpus longan Lour.) and litchi (Litchi chinensis Sonn.) seeds extracts. The longan seed (cultivar “Edor”; LS) and litchi seeds (cultivar “Kim-Cheng”; KC and “Chakkrapat”; CP) were extracted with 95% ethanol with ratio of 1:10 (sample: solvent, w/v) using shaking extraction at 150 rpm, room temperature for 4 hours. Extraction yield, bioactive compounds (extractable phenolic and flavonoid content), antioxidant activities including 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radicals scavenging assay, 2,2’-Azino-bis(3-ethylbenzothialine-6-sulfonic acid) (ABTS) radicals scavenging assay and linoleic acid peroxidation inhibition assays) and antimicrobial activities (inhibition of Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus) of these LS, KC and CP extracts were investigated. The results showed that LS extract composed the high contents of protein and ...
No. O-O-000 Natural Products Research and Development Center (NPRDC), Science and Technology Rese... more No. O-O-000 Natural Products Research and Development Center (NPRDC), Science and Technology Research Institute (STRI), Chiang Mai University, Chiang Mai, 28-30 Nov. 2012 Extraction of bioactive compounds and antioxidant activities from longan (Dimocarpus longan) seeds Pitikan Wisitsak, Junniphaphorn Nimkamnerd, Natthawut Thitipramote, Nisakorn Saewan, Punyawatt Pintathong and Phanuphong Chaiwut Excellent Center for Cosmetics and Wellness, Mae Fah Luang University, Chiang Rai, 57100, Thailand School of Cosmetic Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand E-mail address: [email protected] Introduction and Objective Antioxidant compounds are found in various fruits, vegetables including longan (Dimocarpus longan). In Chiang Rai province, the yield of cultivated longan was around 62,296 tons in year 2010. The enormous by-products (peel and seed) from the fruit processing industry and human consumption are generated (~15% w/w of whole fruit). The aim of prese...
Plant Omics, 2015
In Thailand, there has been growing interest in the use of colored rice extracts as a new source ... more In Thailand, there has been growing interest in the use of colored rice extracts as a new source of anti-oxidative and antiinflammatory effects. This study investigates the effects of different colored rice extracts in terms of their biological content, antioxidative activity, and their ability to reduce pro-inflammatory cytokines and matrix metalloproteinase (MMP) expression. Various colored rice from different rice cultivating areas in Thailand were used to obtain ethanolic extracts. The biological compounds in colored-rice extracts were determined by Folin-Ciocalteu colorimetric and pH-differential methods. To determine the anti-oxidative properties of colored-rice extract, DPPH radical scavenging, ferrous reducing power, and lipid peroxidation assays were used. The cytotoxicity of colored rice extracts was determined by MTT assay on a human promyelocytic leukemia (HL-60) cell line in vitro. The inhibition of pro-inflammatory cytokines (IL-6, TNF-α, NF-κB) and MMP expression in L...
Objective: This research quantified the phytochemical profile, antioxidant activities and cytotox... more Objective: This research quantified the phytochemical profile, antioxidant activities and cytotoxicity of Thai pigmented rice. Methods: Acidified ethanol extracts from seeds of twelve Thai pigmented rice cultivars (six black and six red rice) were investigated for their content of bioactive compounds (phenolics, anthocyanins, and procyanidins), antioxidant activities (ferric reducing antioxidant power; FRAP, DPPH radical scavenging activity; DPPH, and inhibition of lipid peroxidation; TBARS), and cytotoxicity on human promyelocytic leukemia (HL-60) cells. Results: Black rice cultivars (0.05–0.54 mg GAE/ml extract) and red rice cultivars showed total phenolic content (0.21–0.99 mg GAE/ml extract). The predominant phenolic pigments were anthocyanins (1.51±1.70 mg C3G/l extract) for black rice and procyanidins (0.35±0.23 mg EPE/ml) for red rice. Antioxidant activity ranged within 0.06–1.36 mg AAE/ml for FRAP, IC 500.10–1.12 mg/ml for DPPH and 7.57–40.48 % for TBARS. Red rice tended to ...
Lac natural dye is a red color product obtained from female insect Coccus laccae (Laccifer lacca ... more Lac natural dye is a red color product obtained from female insect Coccus laccae (Laccifer lacca Kerr). In Thailand, lac dye is used as a natural red dyestuff for cotton and silk dyeing. Mordants, which is usually different metal salts, can form complexes with the dye molecules via coordinate bonds. These complexes are insoluble and hence improve the dye fastness. Therefore, preparation of lake pigment from Thai lac dye using four metal salts AlK(SO4)2.12H2O, AlCl3, CaCO3, and Ca[CH3COO]2.H2O was investigated. The lac lake was prepared at different conditions such as pH, temperature, reaction time and metal salt concentration. The suitable condition for the lake preparation is of pH 3.5±0.1 at 100 °C, 2h and suitable metal salt is AlK(SO4)2.12H2O and Ca[CH3COO]2.H2O. The UV-Vis spectra of lac lake complex showed the bathochromic shift from that of lac dye which may due to a result of coordination by lone pair electrons on the N or O donor atoms with metal ions, thus stabilizing the ...
In order to investigate the optimal microwave-assisted extraction condition of bioactive compound... more In order to investigate the optimal microwave-assisted extraction condition of bioactive compounds from fresh leaf and stem of Thai water spinach (TW). Bioactive compounds were extracted with ethanol and water by microwave at 450, 600 and 850 watt for 60 seconds on ratio of 1 to 5 (sample: solvent). Phytochemical screening, total flavonoid content (TFC) and DPPH-radical scavenging activity of the extracts were determined. The highest yield was found in ethanolic extract of leaf at 600 watt (1.58%). From phytochemical screening test, all samples showed positive for alkaloids, carbohydrates, terpenes, phenols, tannins and flavonoids. The ethanolic extracts showed higher TFC than those of water extracts in all experiment conditions. The ethanolic stem extracts showed higher DPPH radical scavenging activity than those of aqueous extracts. While the aqueous leaf extracts were higher scavenging activity than those of ethanolic leaf extracts. The significantly highest flavonoid contents we...
Summary. The oils from seeds of Hevea brasiliensis (Muell. Arg.) cultivar RRIM 600 cultivated in ... more Summary. The oils from seeds of Hevea brasiliensis (Muell. Arg.) cultivar RRIM 600 cultivated in Thailand (from two different sources, Chiang Rai and Surin provinces), were subjected to the evaluation of fatty acid composition, antioxidant activities, antimicrobial activities and cytotoxicity. The seed oils were extracted using n -hexane as a solvent and the major fatty acids were oleic and linoleic acids. The seed oils from two different sources similarly exhibited high capability in inhibiting scavenging DPPH radicals (95%, 87% inhibition, from Chiang Rai and Surin provinces respectively), reducing power (1.588±0.016, 1.832±0.009 mg of AAE/mL). However, moderate lipid peroxidation inhibition activity of these two seed oils were observed (24%, 28% inhibition). The cytotoxicity effect of oil was determined on human dermal fibroblast. It showed that the H. brasiliensis seed oil was not cytotoxic to human skin at >1000 μg/mL. Based on these results, it was suggested that the H. bra...
Bee pollen is promoted as a health food with a wide range of nutritional and therapeutic properti... more Bee pollen is promoted as a health food with a wide range of nutritional and therapeutic properties. It contains carbohydrates, lipids, vitamins, and minerals, which contribute to the health effects and flavonoids which are regarded as protective agent. In this work, Mimosa pudica rich bee pollen collected from Chiangrai province, Thailand, was developed as natural antioxidant for topical application. Bee pollen was extracted at 50℃ for 4 hours under sonication in ethanol and propylene glycol solvent. Antioxidant capacities of the extract were investigated by various methods. The free radical-scavenging activities of different solvent extracts of pollen were determined using a 1, 1-diphenyl-2-picrylhydrazine (DPPH) assay and it was found that bee pollen extracted in ethanol had higher DPPH free radical scavenging activity (1%v/v, 53.4%) than that extracted in propylene glycol solvent (1%w/v, 30.6%). The IC 50 value of crude extract is 0.954㎎/ml, however it showed lower scavenging ac...
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Papers by nisakorn saewan