Cibacron Blue F3GA-attached magnetic poly(2-hydroxyethyl methacrylate) [mPHEMA] beads were prepar... more Cibacron Blue F3GA-attached magnetic poly(2-hydroxyethyl methacrylate) [mPHEMA] beads were prepared by suspension polymerization of HEMA in the presence of magnetite (Fe 3 O 4) nanopowder. Average diameter size of the mPHEMA beads was 150-200 lm. The characteristic functional groups of Cibacron Blue F3GAattached mPHEMA beads were analyzed by Fourier transform infrared spectrometer (FTIR) and Raman scattering spectrometer. The lysozyme adsorption and desorption characteristics of Cibacron Blue F3GA-attached mPHEMA beads were also investigated using FTIR and Raman spectroscopic techniques. When the Raman spectrum of lyso-zyme adsorbed mPHEMA is evaluated characteristic Amide-I band appears at 1657 cm 21. The intensity of this band decreases in the spectrum of lysozyme desorbed mPHEMA sample. When the characteristic bands of lysozyme adsorbed and desorbed mPHEMA samples are compared, the band intensities of desorbed sample are lower than those of lysozyme adsorbed sample except for the band appearing at 656 cm 21 (Tyr vCÀ ÀS).
International Journal of Biological Macromolecules, 2007
Magnetic poly(2-hydroxyethyl methacrylate) mPHEMA beads carrying Cibacron Blue F3GA were prepared... more Magnetic poly(2-hydroxyethyl methacrylate) mPHEMA beads carrying Cibacron Blue F3GA were prepared by suspension polymerization of HEMA in the presence of Fe 3 O 4 nano-powder. Average size of spherical beads was 80-120 m. The beads had a specific surface area of 56.0 m 2 /g. The characteristic functional groups of dye-attached mPHEMA beads were analyzed by Fourier transform infrared spectrometer (FTIR) and Raman spectrometer. mPHEMA with a swelling ratio of 68% and carrying 28.5 mol Cibacron Blue F3GA/g were used for the purification of lysozyme. Adsorption studies were performed under different conditions in a magnetically stabilized fluidized bed (i.e., pH, protein concentration, flowrate, temperature, and ionic strength). Lysozyme adsorption capacity of mPHEMA and mPHEMA/Cibacron Blue F3GA beads were 0.8 mg/g and 342 mg/g, respectively. It was observed that after 20 adsorption-desorption cycle, mPHEMA beads can be used without significant loss in lysozyme adsorption capacity. Purification of lysozyme from egg white was also investigated. Purification of lysozyme was monitored by determining the lysozyme activity using Micrococcus lysodeikticus as substrate. The purity of the desorbed lysozyme was about 87.4% with recovery about 79.6%. The specific activity of the desorbed lysozyme was high as 41.586 U/mg.
Cibacron Blue F3GA-attached magnetic poly(2-hydroxyethyl methacrylate) [mPHEMA] beads were prepar... more Cibacron Blue F3GA-attached magnetic poly(2-hydroxyethyl methacrylate) [mPHEMA] beads were prepared by suspension polymerization of HEMA in the presence of magnetite (Fe 3 O 4) nanopowder. Average diameter size of the mPHEMA beads was 150-200 lm. The characteristic functional groups of Cibacron Blue F3GAattached mPHEMA beads were analyzed by Fourier transform infrared spectrometer (FTIR) and Raman scattering spectrometer. The lysozyme adsorption and desorption characteristics of Cibacron Blue F3GA-attached mPHEMA beads were also investigated using FTIR and Raman spectroscopic techniques. When the Raman spectrum of lyso-zyme adsorbed mPHEMA is evaluated characteristic Amide-I band appears at 1657 cm 21. The intensity of this band decreases in the spectrum of lysozyme desorbed mPHEMA sample. When the characteristic bands of lysozyme adsorbed and desorbed mPHEMA samples are compared, the band intensities of desorbed sample are lower than those of lysozyme adsorbed sample except for the band appearing at 656 cm 21 (Tyr vCÀ ÀS).
International Journal of Biological Macromolecules, 2007
Magnetic poly(2-hydroxyethyl methacrylate) mPHEMA beads carrying Cibacron Blue F3GA were prepared... more Magnetic poly(2-hydroxyethyl methacrylate) mPHEMA beads carrying Cibacron Blue F3GA were prepared by suspension polymerization of HEMA in the presence of Fe 3 O 4 nano-powder. Average size of spherical beads was 80-120 m. The beads had a specific surface area of 56.0 m 2 /g. The characteristic functional groups of dye-attached mPHEMA beads were analyzed by Fourier transform infrared spectrometer (FTIR) and Raman spectrometer. mPHEMA with a swelling ratio of 68% and carrying 28.5 mol Cibacron Blue F3GA/g were used for the purification of lysozyme. Adsorption studies were performed under different conditions in a magnetically stabilized fluidized bed (i.e., pH, protein concentration, flowrate, temperature, and ionic strength). Lysozyme adsorption capacity of mPHEMA and mPHEMA/Cibacron Blue F3GA beads were 0.8 mg/g and 342 mg/g, respectively. It was observed that after 20 adsorption-desorption cycle, mPHEMA beads can be used without significant loss in lysozyme adsorption capacity. Purification of lysozyme from egg white was also investigated. Purification of lysozyme was monitored by determining the lysozyme activity using Micrococcus lysodeikticus as substrate. The purity of the desorbed lysozyme was about 87.4% with recovery about 79.6%. The specific activity of the desorbed lysozyme was high as 41.586 U/mg.
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