This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
A total of 231 samples of Puntius sophore ranged 3.0-16cm total length with mean 7.54±1.77 and 0.... more A total of 231 samples of Puntius sophore ranged 3.0-16cm total length with mean 7.54±1.77 and 0.431g body weight with mean 7.14±5.03 from the downstream of Qadirabad barrage, Chenab River, Pakistan were analyzed to investigate some morphometric characters in relation to body length-weight of this fish. Standard length, fork length, head length, head width, body depth, body girth, pre-dorsal length, post-dorsal length and length of caudal peduncle are found to be highly correlated with increasing total length and body weight (r= 0.928; p˂0.01). The slope “b” in the length-weight relationship is 2.63 indicating negative allometric growth. This fish being small in size is heavily targeted by fishermen and local fisher community. This is the first report on any biological aspect of this potentially important small indigenous species from river Chenab. This data will be useful for researchers and fisheries policy makers for future management of this species.
International Journal of Fisheries and Aquatic Studies, 2016
Present study was aimed to access the ichthyofaunal diversity at Head Qadirabad, Chenab, Pakistan... more Present study was aimed to access the ichthyofaunal diversity at Head Qadirabad, Chenab, Pakistan. 1391 fish specimens were collected during September 2015 to June 2016 on monthly basis. 43 species of fishes belonging to seven orders, 14 families and 34 genera were recorded. Overall fish catches were dominated by native fishes along with some exotic fishes like Cyprinus carpio, Ctenopharyngodon idella, Hypophthalmichthys molitrix, Oreochromis niloticus and Oreochromis mossambicus. On the basis of percentage contribution of families to their orders; Siluriformes (35.71%) was found dominant followed by Perciformes (28.57%). Cyprinidae was dominant on the basis of number and percentage contribution of genus and species represented with 14 genera (41.18%) and 19 species (44.19%) followed by Bagridae represented with three genera (8.82%) and four species (9.3%). The values for diversity, richness and evenness indices were 3.36, 13.36 and 0.89 respectively. Effective conservation measures...
Effect of Organophosphate Insectcide Chlorpyrifos on Some Biochemical Components of Trogoderma Granarium Larvae Everts
The stored grain pests are causing huge damage to stored grains world especially tropical regions... more The stored grain pests are causing huge damage to stored grains world especially tropical regions. Different pesticides and fumigation are used for their control. In the presen t study, effect of an organophosphate, chlorpyrifos is being evaluated on Khapara bettle ( Trogoderma granarium) which is a very serious pest in stored grain godones of Pakistan. For this purpose, the LC 50 of chlorpyrifos against 4 th instar larvae of four different strains of T. granarium collected from different cities of Punjab like Khanewal, Lahore, Muzaffargarh and Sheikhupura were determined by residual film method.The LC50 values shown by these strains were 1575.5, 2673.9, 1150.1 and 1790.0 µg /ml res pectively. On the basis of LC50 Lahore strain considered as resistant strain against chlorpyrifos, whereas Muzaffargarh strain used as susceptible strain. Larvae of these two strains of T. granarium were exposed to sub lethal dose (LC 20) for the evaluation of toxic effects of the insecticide on glucose,...
This feeding trial was conducted to investigate the effects of dietary black seed (Nigella sativa... more This feeding trial was conducted to investigate the effects of dietary black seed (Nigella sativa) supplementation on the growth performance, muscles proximate composition, antioxidant and histo-biochemical parameters of rohu (Labeo rohita). Fingerlings (8.503 ± 0.009 g) were fed on 0.0%, 1% and 2.5% black seed supplemented diets for 28 days. Fish sampling was done on the 7th, 14th, 21st and 28th day of experiment. The results of the present study indicated that black seed supplementation significantly increased growth performance and muscles protein contents of rohu over un-supplemented ones. Lipid peroxidation levels significantly decreased in all the studied tissues (liver, gills, kidney and brain) of black seed fed rohu, whereas the antioxidant enzymes (catalase, glutathione-S-transferase, glutathione peroxidase and reduced glutathione) activities were increased in all the studied tissues of black seed supplemented rohu at each sampling day. The hepatic-nephric marker enzymes le...
Diethyl phthalate (DEP) is a widely used low-molecular weight phthalate which is ubiquitously det... more Diethyl phthalate (DEP) is a widely used low-molecular weight phthalate which is ubiquitously detected in almost all kind of the environmental matrices. The aim of the present study was to investigate the impact of DEP-induced toxicity on a suite of biomarker responses in Labeo rohita (rohu). The median lethal concentration of DEP was found to be 4.38 mg L-1 for 96 h. Fingerlings were exposed to a sub-lethal concentration of DEP i.e. 0.51 mg L-1 (1/10 th LC50) for a period of 21 day of the experiment. Oxidative stress and histo-biochemical biomarkers were studied using gills, liver, kidney and brain tissues on 7, 14 and 21 day. The results indicated that DEP-exposure has damaged the antioxidant status by inhibiting the activities of enzymatic stress markers (catalase, glutathione-s-transferase, glutathione peroxidase, reduced glutathione) in all the studied tissues of rohu throughout the study period. Moreover, a significant increase in the levels of lipid peroxidation was observed in all the studied tissues in a time-dependent manner. The levels of hepatic-nephric biomarkers (ALT, AST, ALP, Urea and Creatinine) were found to be significantly elevated for DEP-exposed rohu when compared to control (P<0.05) throughout the study period. The histopathological marker showed severe lesions in the gills (hypertrophy, fusion and curling of lamellae,), liver (pyknotic nuclei, leukocytes infiltration and vacuolization) and kidney tissues (glomerulus expansion, narrowing and elongation of renal tubules) of DEP-exposed rohu. In conclusion, the above mentioned histo-biochemical parameters could be used as biomarkers for DEP toxicity monitoring in the aquatic ecosystem.
Present study was conducted to access fish diversity at head Khanki situated on river Chenab, Pun... more Present study was conducted to access fish diversity at head Khanki situated on river Chenab, Punjab, Pakistan. Various fishing techniques were employed to capture maximum number of fish species from the sampling sites during the period September 2015 to May 2016. A total of 1029 fish specimens belonging to 34 species, 28 genera, 12 families and six orders were recorded from the study area. Order Siluriformes was found dominant contributing five families (41.7%) followed by Perciformes contributing three families (25%) to the present collection. On the basis of number and percentage contribution family Cyprinidae was found dominant contributing 14 genera (50%) and 18 species (52.9%). Overall fish catches were dominated with indigenous species along with four exotic species Cyprinus carpio, Ctenopharyngodon idella, Hypophthalmicthyus molitrix and Oreochromis niloticus. The values for Shannon-Weaver diversity (H'), Margalef's richness and Pielou's (E) evenness indices were 3.30, 10.9 and 0.94 respectively. Various anthropogenic stresses such as aquatic pollution, habitat destruction, and introduction of alien species are responsible for low fish species diversity and evenness at head Khanki. Effective conservation measures are strongly recommended to sustain the populations of commercially important fishes in this river.
Present study was aimed to access the ichthyofaunal diversity at Head Qadirabad, Chenab, Pakistan... more Present study was aimed to access the ichthyofaunal diversity at Head Qadirabad, Chenab, Pakistan. 1391 fish specimens were collected during September 2015 to June 2016 on monthly basis. 43 species of fishes belonging to seven orders, 14 families and 34 genera were recorded. Overall fish catches were dominated by native fishes along with some exotic fishes like Cyprinus carpio, Ctenopharyngodon idella, Hypophthalmichthys molitrix, Oreochromis niloticus and Oreochromis mossambicus. On the basis of percentage contribution of families to their orders; Siluriformes (35.71%) was found dominant followed by Perciformes (28.57%). Cyprinidae was dominant on the basis of number and percentage contribution of genus and species represented with 14 genera (41.18%) and 19 species (44.19%) followed by Bagridae represented with three genera (8.82%) and four species (9.3%). The values for diversity, richness and evenness indices were 3.36, 13.36 and 0.89 respectively. Effective conservation measures are suggested to sustain the population of commercially important fishes.
Marala headwork situated on River Chenab in Punjab, Pakistan was surveyed to assess the diversity... more Marala headwork situated on River Chenab in Punjab, Pakistan was surveyed to assess the diversity and abundance of the fish fauna during the period September 2015 to June 2016. A total of five sampling sites were studied on monthly basis by using different fishing nets for assessing fish diversity. Total 1391 fish specimens belonging to 38 species, 30 genus, 14 families and 6 orders were recorded in the present study. Family Cyprinidae was found dominant with 14 fish species followed by Channidae (4 species) and Bagridae (3 Species). Fish abundance and diversity was assessed by using different diversity indices. The Shannon-Wiener diversity, Pielou’s evenness and Margalef,s richness indices were 2.950, 0.81 and 11.8, respectively. Present findings showed that the population of Tor putitora and Nandus nandus has declined while exotic fish species, Cyprinus carpio, Ctenopharyngodon idella, Hypophthalmicthyus molitrix, Oreochromis niloticus and Oreochromis mossambicus has become established. The low evenness in the river was an indication of the effects of anthropogenic activities such as habitat degradation, aquatic pollution, overfishing, damming and introduction of alien fish species. Present findings highlight the importance of instant mitigation measures for conserving fish diversity in the river. Keywords: Chenab, fish fauna, freshwater, diversity, abundance
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
A total of 231 samples of Puntius sophore ranged 3.0-16cm total length with mean 7.54±1.77 and 0.... more A total of 231 samples of Puntius sophore ranged 3.0-16cm total length with mean 7.54±1.77 and 0.431g body weight with mean 7.14±5.03 from the downstream of Qadirabad barrage, Chenab River, Pakistan were analyzed to investigate some morphometric characters in relation to body length-weight of this fish. Standard length, fork length, head length, head width, body depth, body girth, pre-dorsal length, post-dorsal length and length of caudal peduncle are found to be highly correlated with increasing total length and body weight (r= 0.928; p˂0.01). The slope “b” in the length-weight relationship is 2.63 indicating negative allometric growth. This fish being small in size is heavily targeted by fishermen and local fisher community. This is the first report on any biological aspect of this potentially important small indigenous species from river Chenab. This data will be useful for researchers and fisheries policy makers for future management of this species.
International Journal of Fisheries and Aquatic Studies, 2016
Present study was aimed to access the ichthyofaunal diversity at Head Qadirabad, Chenab, Pakistan... more Present study was aimed to access the ichthyofaunal diversity at Head Qadirabad, Chenab, Pakistan. 1391 fish specimens were collected during September 2015 to June 2016 on monthly basis. 43 species of fishes belonging to seven orders, 14 families and 34 genera were recorded. Overall fish catches were dominated by native fishes along with some exotic fishes like Cyprinus carpio, Ctenopharyngodon idella, Hypophthalmichthys molitrix, Oreochromis niloticus and Oreochromis mossambicus. On the basis of percentage contribution of families to their orders; Siluriformes (35.71%) was found dominant followed by Perciformes (28.57%). Cyprinidae was dominant on the basis of number and percentage contribution of genus and species represented with 14 genera (41.18%) and 19 species (44.19%) followed by Bagridae represented with three genera (8.82%) and four species (9.3%). The values for diversity, richness and evenness indices were 3.36, 13.36 and 0.89 respectively. Effective conservation measures...
Effect of Organophosphate Insectcide Chlorpyrifos on Some Biochemical Components of Trogoderma Granarium Larvae Everts
The stored grain pests are causing huge damage to stored grains world especially tropical regions... more The stored grain pests are causing huge damage to stored grains world especially tropical regions. Different pesticides and fumigation are used for their control. In the presen t study, effect of an organophosphate, chlorpyrifos is being evaluated on Khapara bettle ( Trogoderma granarium) which is a very serious pest in stored grain godones of Pakistan. For this purpose, the LC 50 of chlorpyrifos against 4 th instar larvae of four different strains of T. granarium collected from different cities of Punjab like Khanewal, Lahore, Muzaffargarh and Sheikhupura were determined by residual film method.The LC50 values shown by these strains were 1575.5, 2673.9, 1150.1 and 1790.0 µg /ml res pectively. On the basis of LC50 Lahore strain considered as resistant strain against chlorpyrifos, whereas Muzaffargarh strain used as susceptible strain. Larvae of these two strains of T. granarium were exposed to sub lethal dose (LC 20) for the evaluation of toxic effects of the insecticide on glucose,...
This feeding trial was conducted to investigate the effects of dietary black seed (Nigella sativa... more This feeding trial was conducted to investigate the effects of dietary black seed (Nigella sativa) supplementation on the growth performance, muscles proximate composition, antioxidant and histo-biochemical parameters of rohu (Labeo rohita). Fingerlings (8.503 ± 0.009 g) were fed on 0.0%, 1% and 2.5% black seed supplemented diets for 28 days. Fish sampling was done on the 7th, 14th, 21st and 28th day of experiment. The results of the present study indicated that black seed supplementation significantly increased growth performance and muscles protein contents of rohu over un-supplemented ones. Lipid peroxidation levels significantly decreased in all the studied tissues (liver, gills, kidney and brain) of black seed fed rohu, whereas the antioxidant enzymes (catalase, glutathione-S-transferase, glutathione peroxidase and reduced glutathione) activities were increased in all the studied tissues of black seed supplemented rohu at each sampling day. The hepatic-nephric marker enzymes le...
Diethyl phthalate (DEP) is a widely used low-molecular weight phthalate which is ubiquitously det... more Diethyl phthalate (DEP) is a widely used low-molecular weight phthalate which is ubiquitously detected in almost all kind of the environmental matrices. The aim of the present study was to investigate the impact of DEP-induced toxicity on a suite of biomarker responses in Labeo rohita (rohu). The median lethal concentration of DEP was found to be 4.38 mg L-1 for 96 h. Fingerlings were exposed to a sub-lethal concentration of DEP i.e. 0.51 mg L-1 (1/10 th LC50) for a period of 21 day of the experiment. Oxidative stress and histo-biochemical biomarkers were studied using gills, liver, kidney and brain tissues on 7, 14 and 21 day. The results indicated that DEP-exposure has damaged the antioxidant status by inhibiting the activities of enzymatic stress markers (catalase, glutathione-s-transferase, glutathione peroxidase, reduced glutathione) in all the studied tissues of rohu throughout the study period. Moreover, a significant increase in the levels of lipid peroxidation was observed in all the studied tissues in a time-dependent manner. The levels of hepatic-nephric biomarkers (ALT, AST, ALP, Urea and Creatinine) were found to be significantly elevated for DEP-exposed rohu when compared to control (P<0.05) throughout the study period. The histopathological marker showed severe lesions in the gills (hypertrophy, fusion and curling of lamellae,), liver (pyknotic nuclei, leukocytes infiltration and vacuolization) and kidney tissues (glomerulus expansion, narrowing and elongation of renal tubules) of DEP-exposed rohu. In conclusion, the above mentioned histo-biochemical parameters could be used as biomarkers for DEP toxicity monitoring in the aquatic ecosystem.
Present study was conducted to access fish diversity at head Khanki situated on river Chenab, Pun... more Present study was conducted to access fish diversity at head Khanki situated on river Chenab, Punjab, Pakistan. Various fishing techniques were employed to capture maximum number of fish species from the sampling sites during the period September 2015 to May 2016. A total of 1029 fish specimens belonging to 34 species, 28 genera, 12 families and six orders were recorded from the study area. Order Siluriformes was found dominant contributing five families (41.7%) followed by Perciformes contributing three families (25%) to the present collection. On the basis of number and percentage contribution family Cyprinidae was found dominant contributing 14 genera (50%) and 18 species (52.9%). Overall fish catches were dominated with indigenous species along with four exotic species Cyprinus carpio, Ctenopharyngodon idella, Hypophthalmicthyus molitrix and Oreochromis niloticus. The values for Shannon-Weaver diversity (H'), Margalef's richness and Pielou's (E) evenness indices were 3.30, 10.9 and 0.94 respectively. Various anthropogenic stresses such as aquatic pollution, habitat destruction, and introduction of alien species are responsible for low fish species diversity and evenness at head Khanki. Effective conservation measures are strongly recommended to sustain the populations of commercially important fishes in this river.
Present study was aimed to access the ichthyofaunal diversity at Head Qadirabad, Chenab, Pakistan... more Present study was aimed to access the ichthyofaunal diversity at Head Qadirabad, Chenab, Pakistan. 1391 fish specimens were collected during September 2015 to June 2016 on monthly basis. 43 species of fishes belonging to seven orders, 14 families and 34 genera were recorded. Overall fish catches were dominated by native fishes along with some exotic fishes like Cyprinus carpio, Ctenopharyngodon idella, Hypophthalmichthys molitrix, Oreochromis niloticus and Oreochromis mossambicus. On the basis of percentage contribution of families to their orders; Siluriformes (35.71%) was found dominant followed by Perciformes (28.57%). Cyprinidae was dominant on the basis of number and percentage contribution of genus and species represented with 14 genera (41.18%) and 19 species (44.19%) followed by Bagridae represented with three genera (8.82%) and four species (9.3%). The values for diversity, richness and evenness indices were 3.36, 13.36 and 0.89 respectively. Effective conservation measures are suggested to sustain the population of commercially important fishes.
Marala headwork situated on River Chenab in Punjab, Pakistan was surveyed to assess the diversity... more Marala headwork situated on River Chenab in Punjab, Pakistan was surveyed to assess the diversity and abundance of the fish fauna during the period September 2015 to June 2016. A total of five sampling sites were studied on monthly basis by using different fishing nets for assessing fish diversity. Total 1391 fish specimens belonging to 38 species, 30 genus, 14 families and 6 orders were recorded in the present study. Family Cyprinidae was found dominant with 14 fish species followed by Channidae (4 species) and Bagridae (3 Species). Fish abundance and diversity was assessed by using different diversity indices. The Shannon-Wiener diversity, Pielou’s evenness and Margalef,s richness indices were 2.950, 0.81 and 11.8, respectively. Present findings showed that the population of Tor putitora and Nandus nandus has declined while exotic fish species, Cyprinus carpio, Ctenopharyngodon idella, Hypophthalmicthyus molitrix, Oreochromis niloticus and Oreochromis mossambicus has become established. The low evenness in the river was an indication of the effects of anthropogenic activities such as habitat degradation, aquatic pollution, overfishing, damming and introduction of alien fish species. Present findings highlight the importance of instant mitigation measures for conserving fish diversity in the river. Keywords: Chenab, fish fauna, freshwater, diversity, abundance
Uploads
Papers by maria latif
the fish fauna during the period September 2015 to June 2016. A total of five sampling sites were studied on monthly
basis by using different fishing nets for assessing fish diversity. Total 1391 fish specimens belonging to 38 species, 30
genus, 14 families and 6 orders were recorded in the present study. Family Cyprinidae was found dominant with 14 fish
species followed by Channidae (4 species) and Bagridae (3 Species). Fish abundance and diversity was assessed by using
different diversity indices. The Shannon-Wiener diversity, Pielou’s evenness and Margalef,s richness indices were 2.950,
0.81 and 11.8, respectively. Present findings showed that the population of Tor putitora and Nandus nandus has declined
while exotic fish species, Cyprinus carpio, Ctenopharyngodon idella, Hypophthalmicthyus molitrix, Oreochromis
niloticus and Oreochromis mossambicus has become established. The low evenness in the river was an indication of the
effects of anthropogenic activities such as habitat degradation, aquatic pollution, overfishing, damming and introduction
of alien fish species. Present findings highlight the importance of instant mitigation measures for conserving fish
diversity in the river.
Keywords: Chenab, fish fauna, freshwater, diversity, abundance
the fish fauna during the period September 2015 to June 2016. A total of five sampling sites were studied on monthly
basis by using different fishing nets for assessing fish diversity. Total 1391 fish specimens belonging to 38 species, 30
genus, 14 families and 6 orders were recorded in the present study. Family Cyprinidae was found dominant with 14 fish
species followed by Channidae (4 species) and Bagridae (3 Species). Fish abundance and diversity was assessed by using
different diversity indices. The Shannon-Wiener diversity, Pielou’s evenness and Margalef,s richness indices were 2.950,
0.81 and 11.8, respectively. Present findings showed that the population of Tor putitora and Nandus nandus has declined
while exotic fish species, Cyprinus carpio, Ctenopharyngodon idella, Hypophthalmicthyus molitrix, Oreochromis
niloticus and Oreochromis mossambicus has become established. The low evenness in the river was an indication of the
effects of anthropogenic activities such as habitat degradation, aquatic pollution, overfishing, damming and introduction
of alien fish species. Present findings highlight the importance of instant mitigation measures for conserving fish
diversity in the river.
Keywords: Chenab, fish fauna, freshwater, diversity, abundance