Papers by mahshad khodarahmian
Resveratrol ameliorates mitochondrial biogenesis and reproductive outcomes in women with polycystic ovary syndrome undergoing assisted reproduction: a randomized, triple-blind, placebo-controlled clinical trial
Journal of ovarian research, Jul 10, 2024
High Anti-Müllerian Hormone Strongly Correlates with Reproductive Outcomes in Women Undergoing Assisted Reproduction
Clinical Laboratory, Dec 31, 2022
Frontiers in Nutrition
BackgroundSince the association between dietary quality scores and semen quality remains unclear,... more BackgroundSince the association between dietary quality scores and semen quality remains unclear, we carried out a hospital-based cross-sectional study to investigate the association of Dietary Total Antioxidant Capacity (dTAC), Dietary Inflammatory Index (DII), and Alternative Healthy Eating Index (AHEI) scores with semen quality in men seeking infertility treatment.MethodsThis study enrolled 210 men with unexplained or idiopathic infertility. Semen samples were collected and analyzed according to the WHO 2010 criteria. Dietary data was collected using a 168-item semi-quantitative food frequency questionnaire (FFQ) developed for Tehran Lipid and Glucose Study. Multivariable logistic regression models were used to estimate the relationship between dTAC, AHEI, and DII scores with abnormal semen in crude and adjusted models.ResultsThere were no significant differences across quartile categories of the dTAC, AHEI, and DII scores regarding semen parameters. There was a trend toward a si...
Circulating and Endometrial Profiles of miR-145, miR-155-5p, miR-224, MPP-5, and PECAM-1 Expression in Patients with Repeated Implantation Failure: A Case Control Study
PubMed, Jun 28, 2023
Objective: An association between microRNAs (miRNAs) and adhesion proteins expression with repeat... more Objective: An association between microRNAs (miRNAs) and adhesion proteins expression with repeated implantation failure (RIF) has been recently reported; however, these findings are controversial. This study aims to evaluate the endometrial and circulating expressions of miR-145, miR-155-5p, and miR-224 in addition to the endometrial expressions of membrane protein palmitoylated-5 (MPP-5) and endothelial cell adhesion molecule-1 (PECAM-1) in patients with RIF compared to control subjects. Materials and methods: This case-control study was carried out between June 2021-July 2022. Subjects included 17 patients with RIF and 17 control subjects, who had previous spontaneous term pregnancy with a live birth, who referred to the Medical Centre of Arash Hospital, Tehran, Iran. Endometrial tissue samples were obtained via hysteroscopy and Pipelle catheter in the RIF and control subjects, respectively. Plasma samples were collected after ovulation in all subjects. The expression levels of MPP5, PECAM-1, miR-224, miR-145, and miR-155-5p were evaluated by quantitative real-time polymerase chain reaction (qRT-PCR). The student's t test, chi-square, Mann-Whitney U, and analysis of covariance (ANCOVA) were used for data analyses. Results: RIF patients had less endometrial miR-155-5p expression, and higher endometrial and circulating expressions of miR-145 and miR-224 compared to control subjects. Endometrial PECAM-1 and MPP5 expression significantly decreased in patients with RIF compared to the control group. There was a positive correlation between circulating miR-224 and endometrial miR-155-5p, and between circulating miR-155-5p and endometrial PECAM-1 expression levels in patients with RIF. Conclusion: The present study suggests that circulating miR-224, endometrial miR-145, and PECAM-1 can be reliable, novel biomarkers for diagnosis of RIF.
Cell Journal (Yakhteh), 2021
Objective Sulforaphane (SFN) is a natural free radical scavenger that can reduce oxidative stress... more Objective Sulforaphane (SFN) is a natural free radical scavenger that can reduce oxidative stress (OS) through mediating nuclear factor (erythroid-derived 2)-like 2 (NF-E2-related factor 2 or NRF2)/antioxidant response element (ARE) signaling pathway and the downstream antioxidant enzymes. Here, we intended to study the role of SFN in OS- induced human granulosa cells (GCs) by investigating the intracellular levels of reactive oxygen species (ROS), cell death, and NRF2-ARE pathway. Materials and Methods This experimental study was conducted on GCs of 12 healthy women who had normal menstrual cycles with no history of polycystic ovary syndrome (PCOS), endometriosis, menstrual disorders, hyperprolactinemia, or hormonal therapy. After isolation of GCs, the MTT assay was performed to explore GCs viability after treatment with SFN in the presence or absence of H2O2. Flow cytometry was utilized to determine the intracellular ROS production and the apoptosis rate. Evaluation of the mRNA an...
Öz Amaç: Bu çalışmanın amacı; düzenli adet döngüsü olan kadınlarda modifiye doğal siklus dondurul... more Öz Amaç: Bu çalışmanın amacı; düzenli adet döngüsü olan kadınlarda modifiye doğal siklus dondurulmuş-çözülmüş embriyo transferi (NC-FET) ve yapay siklus (AC)-FET arasında, gebelik sonuçları bakımından bir farklılık olup olmadığını araştırmaktır. Gereç ve Yöntemler: Dahil etme kriterlerini karşılayan ve en az iki embriyosu dondurularak saklanmış 170 hasta, prospektif randomize kontrollü çalışmaya dahil edildi. Bernoulli dağılımı baz alınarak 85 hasta iki gruba randomize edildi: 1) Ovülasyon indüksiyonu için insan koryonik gonadotropin kullanılan modifiye NC-FET ve 2) Endometriyal zamanlamanın östrojen ve progesteron ile programlandığı AC-FET. Çalışılan temel sonuç ölçeği, siklus başına klinik gebelik oranıydı. Bulgular: İki grup arasında; kimyasal, klinik ve devam eden gebelik oranları açısından (sırasıyla; %48,2'ye karşı %45,9, p>0,05; %38,9'a karşı %35,3, p>0,05 ve %37,6'ya karşı %34,1, p>0,05) hem de siklus başına canlı doğum ve düşük yapma oranları açısından ...
JBRA Assisted Reproduction, 2021
Ovarian cancer continues to be the leading cause of death from gynecological cancers. Despite inc... more Ovarian cancer continues to be the leading cause of death from gynecological cancers. Despite inconsistent results, patients with metabolic abnormalities, including obesity and diabetes mellitus (DM), have poorer outcomes, showing a correlation with ovarian cancer incidence and ovarian cancer survival. Since ovarian cancer is the most common cancer in women, and considering the increasing prevalence of obesity and DM, this paper reviews the literature regarding the relationship between the aforementioned metabolic derangements and ovarian cancer, with a focus on ovarian cancer incidence, mortality, and likely mechanisms behind them. Several systematic reviews and meta-analyses have shown that obesity is associated with a higher incidence and poorer survival in ovarian cancer. Although more studies are required to investigate the etiological relation of DM and ovarian cancer, sufficient biological evidence indicates poorer outcomes and shorter survival in DM women with ovarian cancer. A variety of pathologic factors may contribute to ovarian cancer risk, development, and survival, including altered adipokine expression, increased levels of circulating growth factors, altered levels of sex hormones, insulin resistance, hyperinsulinemia, and chronic inflammation. Thus, obesity and DM, as changeable risk factors, can be targeted for intervention to prevent ovarian cancer and improve its outcomes.
Journal of Reproductive Immunology, 2021
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
American Journal of Reproductive Immunology, 2019
Problem: Polycystic ovary syndrome (PCOS) is associated with endoplasmic reticulum (ER) stress an... more Problem: Polycystic ovary syndrome (PCOS) is associated with endoplasmic reticulum (ER) stress and proinflammatory condition. The aim of the present study was to evaluate the effect of resveratrol treatment on proinflammatory and ER stress markers in PCOS patients. Method of Study: Cumulus cells were obtained from 40 PCOS patients who were divided into two groups: placebo and resveratrol treatment (receiving 800 mg/day for 40 days) groups. Blood samples were obtained from all patients before and after the procedure to evaluate interleukin (IL)-6, IL-1β, IL-18, TNF-α, NF-κB, and Accepted Article This article is protected by copyright. All rights reserved. C-reactive protein (CRP). Total RNA was extracted from cumulus cells and cDNA was synthesized by reverse transcription. Expressions of five genes in ER stress response pathway (ATF4, ATF6, CHOP, GRP78, and XBP1s) were assessed with quantitative real-time PCR. Statistical analysis was performed with Student's t-test. Results: After treatment with resveratrol, it was found that serum levels of IL-6, IL-1β, TNF-α, IL-18, NF-κB, and CRP decreased in treatment group. In addition, gene expression results showed that the expression levels of ATF4 (p<0.05) and ATF6 (p<0.001) significantly increased in resveratrol treatment group, while the expression levels of CHOP, GRP78, and XBP1 (p<0.001 for all) significantly decreased. Conclusions: Results demonstrated that resveratrol has anti-inflammatory effects through the suppression of NF-κB and NF-κB regulated gene products. On the other hand, resveratrol can modulate ER stress in granulosa cells (GCs) by altering the expression of genes involved in unfolding protein response (UPR) process. Our findings suggest that ER stress is a potential therapeutic target for PCOS patients.
Astaxanthin treatment decreases pro‐inflammatory cytokines and improves reproductive outcomes in patients with polycystic ovary syndrome undergoing assisted reproductive technology: A randomized clinical trial
Inflammopharmacology, Jun 25, 2024
The modulating effects of vitamin D on the activity of β-catenin in the endometrium of women with endometriosis: a randomized exploratory trial
Gynecological Endocrinology, Dec 11, 2020
Abstract Aims The aim of this prospective study was to investigate the effects of vitamin D on th... more Abstract Aims The aim of this prospective study was to investigate the effects of vitamin D on the expression and activity of β-catenin, as the key molecule of the Wnt/β-catenin signaling pathway, in endometriosis women. Materials and methods Thirty four infertile women with stage III or IV endometriosis were randomly divided to two groups. The control group received the routine treatment and the treatment group, beside the routine protocol, received 50000 IU vitamin D weekly for 12–14 weeks. Blood and endometrial tissue were collected from both groups before and after the intervention. Protein and Gene expression levels of β-catenin were assessed by Western blotting and Real-Time PCR, respectively. Results Compared to before intervention, the expression of active form of β-catenin reduced significantly within treatment group (p = .000), in addition, the difference between control and treatment groups (p = .012) was significant after intervention, too. Also, the ratio of active/total form of β-catenin protein expression was significantly decreased within the treatment group at the end of intervention period (p = .000). Conclusions It seems vitamin D can change the activity of β-catenin protein in the endometrial cells of endometriosis patients. Further studies on the therapeutic potential of vitamin D in modifying the β-catenin activity in endometriosis patients are warranted. Clinical Trial Registration Number IRCT2015081823678N1 Trial registration date 29 September 2015
Complementary Therapies in Clinical Practice, 2017
Objective: To investigate the effects of an antenatal yoga program on perceived maternal labor pa... more Objective: To investigate the effects of an antenatal yoga program on perceived maternal labor pain and delivery outcomes. Material & Methods: This randomized control trial was conducted with sixty primiparous women, aged 18-35 years old, who were randomly assigned to either an antenatal yoga program or control groups. Labor pain and discomfort level of the participants were measured using a Visual Analogue Scale at cervical dilatation of 3-4 c and at 2 and 4 hours after the initial measurement. Demographic and obstetrical information were collected. The antenatal yoga program consisted of a 1-hour supervised yoga class, three times a weekly, starting at 26 weeks gestation.. Results: Participants in control group reported higher pain intensity compared to experimental group at 3-4 cm of dilatation (p=0.01) and at 2 hours after the first and the second measurements (p=0.000). Mothers in the antenatal intervention group that completed the yoga class required a decreased frequency of labor induction in comparison with control group (p=0.008). In addition, mode of delivery of the intervention group resulted in a lower percentage of cesarean section than control group (p=0.002). Lastly, the intervention group experienced a shorter duration of the second and third stages of labor. Interval level data was analyzed by using an independent t-test and chi-square. Conclusion: Yoga during pregnancy may contribute to a reduction pain of labor and improved adequacy of childbirth.
Medical Journal of the Islamic Republic of Iran (MJIRI)
Menstrual disturbances and hormonal changes in women workers exposed to a mixture of organic solv... more Menstrual disturbances and hormonal changes in women workers exposed to a mixture of organic solvents in a pharmaceutical company
Global Journal of Health Science, 2013
Background: In this study, the characteristics of menstrual cycle in shift workers employed in th... more Background: In this study, the characteristics of menstrual cycle in shift workers employed in the pharmaceutical industry are investigated. Method: This study was conducted in a pharmaceutical industrial complex in Tehran in 2012. 406 female workers in packaging units were studied on the menstrual cycle characteristics. The studied workers were divided into two groups of shift workers and non-shift workers and were compared in terms of the frequency of menstrual disorder (short-term cycle, long-term cycle, irregular cycle and bleeding during menstrual cycle) as well as hormonal values (FSH, LH, TSH, and Prolactin). Results: The odds ratio (OR) for menstrual disorder in the shift workers was 5.54 (95% CI=2.78-11.02) compared to the non-shift workers. The mean difference of hormonal values (except prolactin) between shift workers and non-shift workers was not significant (P> 0.05). Conclusion: This study suggests that shift work may disrupt the menstrual cycle.
Journal of Assisted Reproduction and Genetics, 2019
Objectives Management options for PCOS, as the most prevalent endocrine disorder in women of repr... more Objectives Management options for PCOS, as the most prevalent endocrine disorder in women of reproductive age, using natural supplements have a high priority for physicians, especially based on the etiological pathways. Therefore, this study was conducted to describe the effect of resveratrol on the angiogenesis pathway, for management of PCOS through assessing VEGF, HIF1 gene expression, and laboratory parameters. Methods In this triple-blind RCT, PCOS was confirmed in ICSI candidates based on the Rotterdam criteria. Sixty-two patients that met the inclusion criteria were randomly assigned to two groups. All patients took resveratrol 800 mg/day or placebo for 40 days orally from the beginning of their previous menstruation cycle until the oocyte retrieval day. The serum levels of different hormones were measured, and the expression of HIF1 & VEGF genes was quantified by real-time PCR. Results As for the laboratory hormone assay in 61 PCOS patients, a significant mean difference was seen in the FSH, LH, TSH, and testosterone between the two groups (P < 0.05). The results showed a reduction in the expression of VEGF & HIF1 genes under the effect of resveratrol in the granulosa cells (P = 0.0001). The number of mature oocytes, cleavage rate, fertilization rate, and fertility rate were not significantly different between the two groups (P > 0.05), but the high-quality oocyte rate and highquality embryo rate were higher in the resveratrol group (P < 0.05). Conclusions Based on the results, resveratrol may improve some outcomes of PCOS patients, probably through changing the serum levels of some sex hormones and expression of VEGF & HIF1 genes in the angiogenesis pathway of granulosa cells.
Astaxanthin ameliorates inflammation, oxidative stress, and reproductive outcomes in endometriosis patients undergoing assisted reproduction: A randomized, triple-blind placebo-controlled clinical trial
Frontiers in Endocrinology
PurposeIn a randomized, triple-blind, placebo-controlled clinical trial (RCT) including 50 infert... more PurposeIn a randomized, triple-blind, placebo-controlled clinical trial (RCT) including 50 infertile women with endometriosis candidate for assisted reproductive techniques (ART), we studied the effect of Astaxanthin (AST) on pro-inflammatory cytokines, oxidative stress (OS) markers, and early pregnancy outcomes.MethodsBefore and after 12 weeks of AST treatment (6 mg per day), blood serum and follicular fluid (FF) samples were collected from 50 infertile women with endometriosis stage III/IV undergoing ART. Pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) and OS markers (malondialdehyde [MDA], superoxide dismutase [SOD], catalase [CAT], and total antioxidant capacity [TAC]) were measured in the serum and FF. ART outcomes were also compared between the groups.ResultsIncreased serum levels of TAC (398.661 ± 57.686 vs. 364.746 ± 51.569; P = 0.004) and SOD (13.458 ± 7.276 vs. 9.040 ± 5.155; P = 0.010) were observed after AST therapy in the treatment group. Furthermore, serum MDA (14....
Turkish Journal of Obstetrics and Gynecology, 2018
Objective: To investigate whether there was a difference in pregnancy outcomes between modified n... more Objective: To investigate whether there was a difference in pregnancy outcomes between modified natural cycle frozen-thawed embryo transfer (NC-FET) cycles and artificial cycles (AC)-FET in women who all had regular menstrual cycles. Materials and Methods: One hundred seventy patients who met the inclusion criteria and had at least two cryopreserved embryos were included in a prospective randomized controlled trial. Eighty-five patients were randomized based on Bernoulli distribution into the following two groups: 1) Modified NC-FET using human chorionic gonadotropin for ovulation induction and 2) AC-FET, in which endometrial timing was programmed with estrogen and progesterone. The main studied outcome measure was the clinical pregnancy rate per cycle. Results: No significant differences were found between the two groups with regard to the chemical, clinical, and ongoing pregnancy rates (48.2% vs 45.9%, p>0.05; 38.9% vs 35.3%, p>0.05; and 37.6% vs 34.1%, p>0.05, respective...
The Journal of Steroid Biochemistry and Molecular Biology
Highlights The endometrium of endometriosis patients expresses higher levels of CD44s, CD44V, a... more Highlights The endometrium of endometriosis patients expresses higher levels of CD44s, CD44V, and CD44v6. The endometrial fluid and serum concentration of sCD44 is higher in endometriosis patients. Vitamin D down regulates the expression of CD44s, CD44V, and CD44v6 in the endometriosis patients. Vitamin D regulates the shedding of sCD44 in the endometrium of endometriosis patients.
Medical journal of the Islamic Republic of Iran, 2014
Chemicals are among risk factors that can affect women's reproductive system. This study is a... more Chemicals are among risk factors that can affect women's reproductive system. This study is aimed to investigate the association of occupational exposure to a mixture of organic solvents with menstruation disturbances and hormonal changes among female workers. Female workers of a pharmaceutical company were divided into three groups of non-exposed, lowexposed and highly-exposed to a mixture of organic solvents (formaldehyde, phenol, N-hexane, and chloroform) based on workplace measurements. Menstrual disturbances (in terms of short cycles, long cycles, irregular cycles, and bleeding or spotting between periods) and mean of hormone levels (including follicle stimulating hormone, luteinizing hormone, thyroid stimulating hormone, prolactin, estrogen and progesterone levels) were compared between these three groups. For investigating associations, logistic regression was performed. Our study showed that mean length of cycles, duration of bleeding, and amount of flow and also prevale...
The role of antioxidants in sperm freezing: a review
Cell and Tissue Banking, 2016
Cryopreservation of spermatozoa is becoming more important because of new clinical requirements a... more Cryopreservation of spermatozoa is becoming more important because of new clinical requirements and current clinical practice. Despite the success of sperm cryopreservation this routinely used procedure induces serious detrimental changes in sperm function. Some researchers believe that cryopreservation is associated with DNA fragmentation and DNA single strand breaks in sperm. Mechanisms of cryodamage to human spermatozoa are thought to be multifactorial including: cold shock, osmotic stress, intracellular ice crystal formation, oxidative stress, and combinations of these conditions. Additives showing antioxidative properties reported to reduce the impact of ROS-induced and cold shock damages. Many studies exist as regards the effects of antioxidants on the cryopreservation aimed at improving the quality of post-thaw semen. Hence, this review will clarify results of recent applications of various antioxidants used in numerous research efforts to improve cryopreservation of spermatozoa. This review is to increase the understanding of the roles of these antioxidants concerning mechanisms which enhance resistance to cryodamage of spermatozoa.
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Papers by mahshad khodarahmian