Papers by kahin shahanipour
Biochemical genetics, Apr 7, 2024
Russian Journal of Bioorganic Chemistry
مجله دانشکده پزشکی اصفهان, 2015
Background: Considering the prevalence of thyroid dysfunction and diseases such as Hashimoto'... more Background: Considering the prevalence of thyroid dysfunction and diseases such as Hashimoto's thyroiditis and Graves' disease in the Dezful city, Iran, this study aimed to evaluate the effect of Hashimoto's thyroiditis on serum levels of glutathione and iron and the activity of caspase-3. Methods: In this case-control study, 44 patients with Hashimoto's thyroiditis and 44 healthy controls were studied. The serum levels of glutathione and iron and the activity of caspase-3 were measured. The results were analyzed using Mann-Whitney test via SPSS software. Findings: The amount of serum Fe was significantly lower in patients (P = 0.041). The glutathione level in healthy people was lower; but tests showed no significant difference (P = 0.502). The caspase-3 enzyme activity in healthy subjects was lower; but Mann-Whitney test showed no significant difference (P = 0.089). Conclusion: In patients with Hashimoto's thyroiditis in hypothyroidism phase, the serum iron leve...
Article history: Received 25 March 2014 Received in revised form 25 April 2014 Accepted 23 May 20... more Article history: Received 25 March 2014 Received in revised form 25 April 2014 Accepted 23 May 2014 Available online 16 June 2014
DOAJ (DOAJ: Directory of Open Access Journals), Jul 1, 2022
Journal of Physical & Theoretical Chemistry, Feb 1, 2010
Iranian Journal of Endocrinology and Metabolism, Jan 10, 2020
Nanomedicine Journal, Apr 1, 2018
How to cite this article Jarahian A, Fatahian S, Shahanipour K. Investigation the effect of Fe 3 ... more How to cite this article Jarahian A, Fatahian S, Shahanipour K. Investigation the effect of Fe 3 O 4 nanoparticles on liver and stress oxidative parameters at the presence of magnetic field in rat.
journal of north khorasan university of medical sciences, Jul 1, 2016
Background & objective: Honey and Aloe vera have traditionally used for its healing effects on bu... more Background & objective: Honey and Aloe vera have traditionally used for its healing effects on burn wounds. The aim of this study was to investigate the effect of honey and aloe vera on the burn wound healing process in rat. Materials & Methods: This was an experimental study performed on 24 adult male rats. Following ether anesthesia, a second degree burn wound of 250mm2 was made on back of each rat. After confirming burns, the rats were divided into 4 groups (6 in each group).Control group, group 1 treated with Honey and Aloe vera, group 2 treated with Aloe vera and group 3 treated with Honey three times per day. Evaluation was made through measuring the area of the healed region and data were analyzed using one way ANOVA test. Results: This study was indicated of a decrease of 123 ±34/024 mm ٢ in burn wounds size after treatment in honey and Aloe vera group which was greater than those in others(P-value =0/009) and honey group was only shown to have decreased the burn wounds size greater than the Aloe vera group. Conclusion: Combination of honey and aloe vera was found to have more healing potential on second-degree burn wound. Faster healing process, less complications and easy availability of honey and Aloe vera are among the reasons suggesting more clinical trials using this preparation.
Introduction: Vaccinium arctostaphylos is a medicinal plant used for the treatment of diabetes. T... more Introduction: Vaccinium arctostaphylos is a medicinal plant used for the treatment of diabetes. The present study was conducted to investigate the effect of the methanol extract of Caucasian whortleberry on glucose, oxidative biomarkers, HDL and cholesterol. Materials and methods: The present experimental study was conducted on 24 male Wistar rats divided into a healthy control group, a diabetic control group and two diabetic trial groups treated with 150 and 250 mg/kg of body weight doses of the methanol extract of Caucasian whortleberry. After inducing diabetes in the rats, the effect of 30 days of intraperitoneal injection of the extract was examined on their blood biochemical markers. Results: Comparing the two diabetic trial groups treated with Caucasian whortleberry extract revealed the 250 mg/kg dose to be the most effective concentration for reducing glucose, increasing HDL, increasing paraxonase activity, reducing malondialdehyde and reducing body weight, while the 150mg/kg dose of the extract was the most effective for reducing cholesterol and protein carbonyl (P<0.05). Conclusion: The present study showed that the methanol extract of Caucasian whortleberry can reduce the adverse effects of diabetes on many biochemical markers and thus boasts medicinal properties.
Journal of Food Biochemistry, Nov 12, 2022
Biocatalysis and agricultural biotechnology, Oct 1, 2020
Abstract The beneficial effects of antioxidant production activity during kombucha tea fermentati... more Abstract The beneficial effects of antioxidant production activity during kombucha tea fermentation elevate the nutritional values of this product. The present study aimed to enhance antioxidant properties and invertase activity in kombucha tea and investigate the synergistic effects of both properties. Following preparation of kombucha solution, the Plackett–Burman design was used for determination of factors affecting the antioxidant and invertase activities in the product. Samples were collected from the fermenting solution on days 0, 7, 10 and 14. The total phenol content of samples was determined using the Folin–Ciocalteau method. In addition, antioxidant activity was measured using cupric ion reducing antioxidant capacity and DPPH (1,1-diphenyl 2-picrylhydrazyl) radical inhibition method. Invertase activity was determined in the samples using sigma protocol. After screening design, the dried starter containing the kombucha tea microbial consortium optimally produced using the vacuum freeze drying method. The results showed that pH of kombucha reduced as the fermentation time passed. Antioxidant activity increased in most samples over time, so that the average antioxidant activity was in the range of 0.99–224.86 nm of trolox equivalents/ml during fermentation. Invertase activity also increased in the most samples during fermentation and showed the range of 50.85–115.5 u/ml. By increasing the activity of invertase, the antioxidant activity of kombucha solution increased. The results of present study proposed that by induction of invertase activity in kombucha drink its effectiveness that resulted by antioxidant properties would be promoted and the drink will be more useful for diabetic patients.
scientific journal of ilam university of medical sciences, Jul 15, 2015
Journal of Food Processing and Preservation, May 23, 2022
New cell-culture biotechnology enables us to culture cancer cells in vitro and to find, design, a... more New cell-culture biotechnology enables us to culture cancer cells in vitro and to find, design, and manufacture new drugs. Active ingredients in herbal medicines enjoy biological balance because other materials accompany them and, hence, these medicines do not cause side effects for the human body and, accordingly, have substantial superiority and advantage over chemical drugs. Assa-foetida gum was extensively used in traditional Iranian medicine. Plants of the same species that grow in different habitats have different percentages of active ingredients depending on the water, air, climatic conditions, and even angle of incidence in their habitats. Therefore, this study was conducted to evaluate and compare cytotoxic effects of methanol extracts prepared from assa-foetida gum obtained from South Khorasan and Yazd Provinces on the breast cancer cell line MDA-MB-231. Methods and Materials: Assa-foetida gum was collected from South Khorasan and Yazd and its methanol extract was prepared. The cancer cell line MDA-MB-231 was cultured on RPMI-1640 culture medium containing 10% fetal bovine serum and 5% CO2 and was incubated in the presence of various methanol extract concentrations for 24, 48, and 72 hours. The MTT method was employed to calculate the survival rate of cells. Discussion and Conclusions: Results indicated that, of the three exposure intervals of 24, 48, and 72 hours, methanol extracts of assa-foetida gum from South Khorasan and Yazd had their maximum cytotoxic effect on the cancer cell line MDA-MB-231 at the 48-hour exposure interval.
Journal of Food Biochemistry, Feb 3, 2023
Te objective of this study was to determine the efect of Trachyspermum ammi (T. ammi) on the live... more Te objective of this study was to determine the efect of Trachyspermum ammi (T. ammi) on the liver of streptozotocin (STZ)induced diabetic rats. Tis study began by identifying the methanolic composition of T. ammi. Te presence of three compounds was determined using gas chromatography-mass spectrometry (GC-MS), with Tymol (60.705%), Υ-Terpinene (22.216%), and Pcymene (17.078%) being the most prevalent. Afterwards, diabetic rats were treated with T. ammi (200-500 mg/kg) or losartan (20 mg/kg) daily for 60 days. In the diabetic rats treated with T. ammi, levels of hepatic indicators, lipid peroxidation markers, and proinfammatory mediators decreased signifcantly. In the liver of T. ammi-treated diabetic rats, glutathione-S-transferase (GST), catalase (CAT), and glutathione (GSH) increased signifcantly. Despite the fact that proinfammatory cytokine levels were signifcantly increased, interleukin-1β (IL-1β) and tumor necrosis factor-alpha (TNF-α) levels decreased in the groups administered the extract. From these results, it can be concluded that T. ammi signifcantly restored the liver's antioxidant balance and infammation caused by hyperglycemia.
Journal of Shahrekord University of Medical Sciences, Sep 8, 2022
Background and aims: Highly active oxygen causes DNA or protein damage. This study aimed to deter... more Background and aims: Highly active oxygen causes DNA or protein damage. This study aimed to determine the effect of the extract of three medicinal plants, royal jelly, and metformin on the survival of human dermal fibroblast (HDF) cells under oxidative stress induced by H 2 O 2. Methods: Hydroalcoholic extracts were prepared from Nigella sativa, Carum carvi seeds, and Taraxacum officinale flower. The growth curve of HDF cells was plotted in Dulbecco's modified eagle medium (DMEM). The cells were exposed to H 2 O 2 stress and treated with different concentrations of the extracts, royal jelly, and metformin. The cells viability was then calorimetrically analyzed during different times in 69-well plates. Results: The exponential growth phase of HDF cells was observed during 24-72-hour growth. High cell survivals were seen during 72 hours of exposure to 25 mg/mL royal jelly (99.13 ± 3.22%), 15 mg/mL C. carvi extracts and N. sativa (97.87 ± 3.25% and 96.22 ± 5.47%, respectively), 10 mg/mL royal jelly (96.14 ± 4.72%), 16 mg/mL metformin (91.23 ± 5.34%), and 8 mg/mL T. officinale (86.31 ± 7.44%), respectively. These results were significantly higher than the results of 48 and 24 hours of exposure to 15 mg/mL N. sativa (P = 0.013), 15 mg/mL C. carvi (P = 0.019), and 16 mg/mL metformin (P = 0.027). Moreover, royal jelly (25 mg/mL) significantly indicated greater effect on the cell survival compared with the highest concentrations of the extracts and metformin at different exposure times (P = 0.016-0.032). Conclusion: Royal jelly, N. sativa, and T. officinale extracts revealed remarkable effects on the stressed HDF cells; therefore, they can be proposed for treatment of wounds and burns after clinical analysis.
Hormozgan Medical Journal, Sep 30, 2019
Background: Cardiovascular disease (CVD) and inflammation are among the most common causes of mor... more Background: Cardiovascular disease (CVD) and inflammation are among the most common causes of mortality in dialysis patients. Factors such as chronic inflammation, dyslipidemia and elevated homocysteine levels may underlie the increased risk of CVD. Objectives: This study aimed to investigate levels of inflammatory markers, homocysteine, lipid profile, WBC, and BMI levels in hemodialysis patients compared with the control subjects in Isfahan. Methods: A total of 32 hemodialysis patients and 32 healthy subjects were selected randomly and their serum levels of IL-6, CRP, homocysteine, and lipid profiles were measured. Results: According to the results, most of the studied hemodialysis patients compared to the healthy subjects had non-normal amounts of IL-6, CRP, and homocysteine i.e., higher than healthy subjects (P < 0.05). Cholesterol, HDL, and LDL were significantly lower than the healthy subjects (P < 0.05), while triglycerides and VLDL levels showed no significant difference (P: 0.38 > 0.05). A significant difference of BMI was observed between the normal subjects and the patients; thus, this factor in patients was less than what was in the healthy group (P: 0.001 < 0.05). Conclusions: The results demonstrated that in most patients the levels of factors IL-6, CRP, and homocysteine were abnormal compared to the healthy group. In addition, the risk of developing cardiovascular and inflammatory disease as well as dyslipidemia in these patients is higher than normal.
Uploads
Papers by kahin shahanipour