Papers by kanchan Srivastava
Reversible hyperpigmentation as the first manifestation of dietary vitamin B12deficiency
Indian Journal of Dermatology, Venereology and Leprology, 2006
A 34-year-old Indian male presented with darkening of skin and discoloration of hair and nails fo... more A 34-year-old Indian male presented with darkening of skin and discoloration of hair and nails for two months. Darkening initially started on the palms and feet [Figure - 1] and then gradually involved the face, gums and tongue. He also complained of diarrhea, decreased appetite, ...
Paripex Indian Journal Of Research, 2018
A retrospective analysis of all cancer patients attending radiation oncology Outpatient departmen... more A retrospective analysis of all cancer patients attending radiation oncology Outpatient department (OPD) of a single unit from year 2009 up to 18 th June 2018 was conducted to find geographical distribution and incidence of most common cancers and hospital load .
Enumeration of T and B lymphocytes and cellular types in various grades of leprosy patients
An Evaluation of Effects of Type-II Diabetes Mellitus on Salivary Flow Rate and Some Salivary Parameters (Total Protein, Glucose, and Amylase) in a Tertiary Care Hospital in Uttar Pradesh, India
BMC Public Health, 2021
Background Uttar Pradesh (UP), India continues to have a high burden of mortality among young chi... more Background Uttar Pradesh (UP), India continues to have a high burden of mortality among young children despite recent improvement. Therefore, it is vital to understand the risk factors associated with under-five (U5) deaths and episodes of severe illness in order to deliver programs targeted at decreasing mortality among U5 children in UP. However, in rural UP, almost every child has one or more commonly described risk factors, such as low socioeconomic status or undernutrition. Determining how risk factors for childhood illness and death are understood by community members, community health workers and facility staff in rural UP is important so that programs can identify the most vulnerable children. Methods This qualitative study was completed in three districts of UP that were part of a larger child health program. Twelve semi-structured interviews and 21 focus group discussions with 182 participants were conducted with community members (mothers and heads of households with U5 c...
Biochemical and biophysical research communications, Dec 16, 2017
Early secretory antigenic target protein (ESAT-6) is an important virulent factor which plays a c... more Early secretory antigenic target protein (ESAT-6) is an important virulent factor which plays a crucial role in Mycobacterium tuberculosis (MTB) pathogenesis. Here, we demonstrate the role of ESAT-6 in phagocytosis and intracellular survival of mycobacteria through a mechanism mediated by regulation of a host protein; Peroxiredoxin-1 (Prdx-1). Prdx-1 is an anti-apoptotic and stress response protein which protects cells from damage by ROS and H2O2. The J774 A.1 cells infected with MTB or over-expressing ESAT-6 through eukaryotic promoter vector showed elevated expression of Prdx-1. Further investigation revealed that the up-regulation of Prdx-1 is mediated through the activation of one of the MAP kinases, p38. The NRF-2, a transcriptional activator of Prdx-1 is translocated to the nucleus upon phosphorylation by p38 and subsequently, regulates expression of Prdx-1. Inhibition of the p38 MAPK by a specific inhibitor, SB203580, abrogates the ESAT-6 mediated induction of Prdx-1 expressi...
Clinical and mutational profile of extensively drug resistant tuberculosis cases in northern India
Tuberculosis and non-tuberculous mycobacterial diseases, 2021
The M. tuberculosis HAD phosphatase (Rv3042c) interacts with host proteins and is inhibited by Clofazimine
Cellular and molecular life sciences : CMLS, Sep 17, 2016
Mycobacterium tuberculosis codes for a HAD-phosphatase, Rv3042c (MtSerB2), that has earlier been ... more Mycobacterium tuberculosis codes for a HAD-phosphatase, Rv3042c (MtSerB2), that has earlier been characterized as a metabolic enzyme. Here we demonstrate that MtSerB2 is secreted into the cytosol of infected macrophages and is found in bronchoalveolar lavage samples of tuberculosis patients. MtSerB2 induces significant cytoskeleton rearrangements through cofilin activation and affects the expression of genes that regulate actin dynamics. It specifically interacts with HSP90, HSP70 and HSP27 that block apoptotic pathways and not with other HSPs. It actively dephosphorylates MAPK-p38 and NF-kappa B p65 that play crucial roles in inflammatory and immune responses. This in turn leads to down-regulation of Interleukin 8, a chemotactic and inflammatory cytokine. Finally, during evaluation of inhibitors against MtSerB2 we found that Clofazimine, a drug being evaluated for XDR and MDR tuberculosis, inhibits MtSerB2 phosphatase activity and reverses the above effects and interactions with ho...
Clinical, demographic and radiological profile of smoker COPD versus nonsmoker COPD patients at a tertiary care center in North India
Journal of Family Medicine and Primary Care
Introduction: Cigarette smoking is the most predominant risk factor for development of chronic ob... more Introduction: Cigarette smoking is the most predominant risk factor for development of chronic obstructive pulmonary disease (COPD). However, a considerable amount of patients do develop COPD without exposure to cigarette smoking. We aimed to analyze the incidence, demographic and clinical profile of nonsmoker COPD subjects at a tertiary care center. Methods: In this prospective observational study, 410 patients were screened for dyspnea. On the basis of spirometry findings, 360 patients were diagnosed as COPD and enrolled into the study. Patients were categorized into 2 groups on the basis of smoking habits (smoker and nonsmoker COPD). Clinical and demographic attributes were compared in between these two groups. This study was conducted over a period of one year, from August 2014 to July 2015. All statistical analyses were performed using Statistical Package for the Social Sciences version 19.0 (SPSS Inc, Chicago, IL, USA). Values were considered to be statistically significant at...
Early secretory antigenic target protein (ESAT-6) is an important virulent factor which plays a c... more Early secretory antigenic target protein (ESAT-6) is an important virulent factor which plays a crucial role in Mycobacterium tuberculosis (MTB) pathogenesis. Here, we demonstrate the role of ESAT-6 in phagocytosis and intracellular survival of mycobacteria through a mechanism mediated by regulation of a host protein; Peroxiredoxin-1 (Prdx-1). Prdx-1 is an anti-apoptotic and stress response protein which protects cells from damage by ROS and H 2 O 2. The J774 A.1 cells infected with MTB or over-expressing ESAT-6 through eukaryotic promoter vector showed elevated expression of Prdx-1. Further investigation revealed that the up-regulation of Prdx-1 is mediated through the activation of one of the MAP kinases, p38. The NRF-2, a transcriptional activator of Prdx-1 is translocated to the nucleus upon phosphorylation by p38 and subsequently, regulates expression of Prdx-1. Inhibition of the p38 MAPK by a specific inhibitor, SB203580, abrogates the ESAT-6 mediated induction of Prdx-1 expression as well as the phosphorylation of NRF-2 in a time-dependent manner. The inhibition of Prdx-1 expression by specific siRNA in J774 A.1 cells resulted in the reduced bacterial uptake and intracellular survival of the mycobacteria. This is the first report proclaiming that the ESAT-6 regulates Prdx-1 which is involved in the increase of mycobacterial uptake and survival. The intermediate mechanisms involve the increased Prdx-1 production in macrophages through the activation of p38 and NRF-2 dependent signaling.
A Educação como Diálogo Intercultural e sua Relação com as Políticas Públicas 3, 2020
Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atrib... more Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição 4.0 Internacional (CC BY 4.0). O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.
Velocidade e Legibilidade De Escrita Manual De Escolares Com Dislexia Do Subtipo Visual, Fonológico e Misto
Fundamentos Científicos e Prática Clínica em Fonoaudiologia, 2021
EDUCATION SCIENCES AND SOCIETY, 2018
The purpose of this paper is to analyze possible correspondences between new technologies and inc... more The purpose of this paper is to analyze possible correspondences between new technologies and inclusive practices for students with difficulties. This perspective allows authors to describe the use of Technology as a possible component of plurals educative settings, able to draw every actor involved on a variety of resources. The targeted use of technologies, intended as a potential, can allow the development and the improvement of inclusive dynamics within the educative field. Specifically this paper presents the technological resources which have enabled the inclusion of students with learning disorders, dyslexia and attention deficit hyperactivity disorders, in the Brazilian scenario.
IOSR Journal of Dental and Medical Sciences, 2013
To study the rate of specimens rejection received in the laboratory. Design: A cross-sectional su... more To study the rate of specimens rejection received in the laboratory. Design: A cross-sectional survey was conducted over a period of six months across Community Health Centres (CHC), Primary Health Centres (PHC), District hospitals and Govt. multi-specialty tertiary care hospital and trust autonomously run charity hospital. In-depth interviews were also conducted with the laboratory staff. Rejection of samples with the aim of proper processing of the samples and for providing report of high standard comes under good lab practices. A total of 2000 sample were studied and followed. A total of 5.3% samples were rejected. The rejection rate was higher among the hospitals run by trusts than govt. In all, the rejection rate was higher blood sample (9.1%) as compared to body fluid (8%), urine (6.8%), stool (5.3%) and sputum (3.3%) sample. The main reason of rejection was due to inadequacy of specimen collection by the paramedical staff. The rejection rate was higher in trust hospitals due to higher awareness at the analytical level of the sample processing in the lab as compared to govt. run hospitals where every sample is processed irrespective of its adequacy/inadequacy and the report is provided. Thus, the emphasis should be given to make such diagnostic kits in future which are less dependent collection and handling.
Direct Detection and Identification of Mycobacterium
Journal of clinical …, 2005
Mycobacterium tuberculosis and M. bovis infect animals and humans. Their epidemiologies in develo... more Mycobacterium tuberculosis and M. bovis infect animals and humans. Their epidemiologies in developed and developing countries differ, owing to differences in the implementation of preventive measures (World Health Organization, 1999). Identification and differentiation of these ...
Proteome Based Insights in Drug-Resistant Mycobacterium tuberculosis
IS6110 an Ineluctable Molecular Marker in Clinical Isolates of Drug Resistant Tuberculosis
Background: Mycobacterium tuberculosis (MTB) seems to have found a liking for us human beings. Th... more Background: Mycobacterium tuberculosis (MTB) seems to have found a liking for us human beings. The bacteria have been known to infect humans since the Vedic era and still continue to create havoc among medical and research community with no chance of resurgence in the near future. According to World Health Organization (2016), 0.48 million people are affected with MDR-TB globally, of which approximately 0.19 million have died. The Indian scenario is not so bright as well with 1/3 rd of the MDR patients belonging to India and 2 TB patients dying every 5 minutes. Materials and Method: Drug susceptibility testing and genotyping of confirmed TB cases were done to identify for the polymorphism in IS6110.. The work was designed as an analytical cross-sectional study. Result: Our study has documented the importance/usefulness of IS6110 in identifying DR-TB strains in clinical samples. Most isolates were resistant to single drug whereas few were to multidrug as well. Conclusion: The emergen...
To study the awareness level and psycho-social impact of Tuberculosis on a Patient's life
International journal of biomedical research, 2017
Background: Tuberculosis (TB) is a major health problem worldwide, not only because of current ca... more Background: Tuberculosis (TB) is a major health problem worldwide, not only because of current cases of active disease and emergence of drug resistant TB (DR-TB) but also because of the threat of latency and co infection with HIV. The major reason behind this is the lack of proper knowledge about the disease among patients and people in general. They are unaware about adhering to the treatment, mode of transmission of the disease and as such are prone to transmit it to others. Lack of knowledge about treatment plan leads to relapse. An important aspect of TB is its socio-psychological effect on the patient. Methods: The work set-up was a prospective questionnaire based study from Feb 2016 to July 2016 conducted by third year undergraduate medical students in TB patients registered under RNTCP center of defined King Georg’s Medical University, UP, Lucknow (KGMU-RNTCP) for directly Observed treatment short course regimen (DOTS). This study was carried out on 150 TB patients. The rat...
Tuberculosis (TB) is an airborne communicable disease plaguing human populations since antiquity.... more Tuberculosis (TB) is an airborne communicable disease plaguing human populations since antiquity. TB continues to be a major public health problem globally and India has one of the largest numbers of TB cases in the world. The risk of progression from exposure to tuberculosis bacilli to the development of active disease is a two-stage process governed by both exogenous and endogenous risk factors. Exogenous factors play a key role in accelerating the progression from exposure to infection, whereas endogenous factors lead in progression from infection to active TB disease. Socioeconomic factors are also shown to increase the susceptibility to infection. Along with emerging variants such as indoor air pollution and tobacco smoke, it plays a significant role at both the individual and population level. Environmental conditions playing a role in disease development were humidity and number of people living in the house. Overcrowded housing and poor ventilation increase both the likeliho...
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Papers by kanchan Srivastava