Papers by HOSSANUR MONDAL
Journal of Drug Delivery Science and Technology, 2019
Abstract In the present work, nanoconjugates of shRNA-plasmid and a non-viral nanoceramic vector,... more Abstract In the present work, nanoconjugates of shRNA-plasmid and a non-viral nanoceramic vector, e.g., Mg–Al layered double hydroxide (Mg-Al LDH), were synthesized and intercalated. Subsequently, these particles with an average size of 40–60 nm, were transfected into mammalian neuroblastoma cells (SH-SY5Y). The as prepared Mg–Al LDH was able to protect the incorporated shRNA-plasmid against a range of pH values, DNaseI, endonucleases, and serum components. To test the applicability of the nanoconjugate for future in-vivo studies, serum from three different model experimental animals viz, mouse, rat and guinea pig was used for the serum protection study. Additionally, we showed that prolonged storage at different temperatures does not affect the quality of the nanoconjugate. Using this nanoconjugate to transform cells, a maximum internalization of ∼26% at 24h was achieved. Lastly, we demonstrated effective and safe delivery of the plasmid by measuring GFP production and shRNA-induced knockdown of TNF alpha.
Hossanur Mondal, 2023
Essay writing is very important for all school children. It helps in various skill development. T... more Essay writing is very important for all school children. It helps in various skill development. The word 'essay' is basically a Latin word 'exagium', which means representing one side of an argument or thoughts or experience in writing. So, an essay is basically a short piece of writing from one's perspective. For the students of Class 3, essay writing helps to gain them a perspective and improve their critical thinking ability.
HOSSANUR, 2023
There has been tremendous growth in the use of the mobile phones in India. It is reported that th... more There has been tremendous growth in the use of the mobile phones in India. It is reported that the India's telecommunication market is the second largest in the world. The mobile phones are available to the people right from the age of 12 years. The mobile phone technology has brought the world closer. It provided great convenience in communication among people by way of either calling or texting. Now, the mobile phones are coming up with variety of features like internet access, sending e-mails, games, access to social networking sites like face-book, listening to music, playing radio, reading books, dictionary and so on. The mobile phones are also used to overcome the feeling of loneliness. The majority of the users are in the age group of 15 to 25 years. The contacts are established instantly with the help of mobile phones which was not possible earlier. However, though the mobile phone provided many advantages, it has also caused some problems also. Some people are using the mobile phones so excessively that it assumes the form of addiction. The use of mobile phones has reduced the face to face communication. It is observed that the people sending text messages while talking to others.
International Journal of Applied Glass Science, 2020
The present communication reports the synthesis of silver containing bioactive glass by sol gel r... more The present communication reports the synthesis of silver containing bioactive glass by sol gel route, followed by fabrication of the antibacterial bioactive glass nanofibers (ABGnf, dia 200-900 nm) using electrospinning technique. The ABGnf of composition (1-2) mole% of B 2 O 3 , (68-69) mole% of SiO 2 , ~1×10-3 mole% of Ag 2 O, and 29-30 mole% of CaO was evaluated both for wound healing potential and for antibacterial efficacy. In an in vitro cell proliferation/migration assay was performed using SV transformed GM00637 (skin fibroblast) cell line, wherein ABGnf exhibited significant cell proliferation (82%) compared to the control (47%) and ABGnf without boron (65%) in a period of 24 h, thus establishing its wound healing potential. Further, the antibacterial activity was assessed in vitro using the bacterial strain of Staphylococcus aureus. The results showed the zone of inhibition to be almost two times the control group treated with a model aminoglycoside antibiotic, tobramycin. In addition to the above advantages, the in vitro cytotoxicity assay showed an excellent cellular response. All these results have established promising wound healing and antibacterial potential of ABGnf.
Applied Clay Science, 2020
The present research reports a facile synthesis of bare phase pure CaAl-LDH nanoparticle (sample ... more The present research reports a facile synthesis of bare phase pure CaAl-LDH nanoparticle (sample A) and intercalation of anti-cancer drug etoposide (ETO) into the same via simple anion exchange technique to obtain ETO loaded CaAl-LDH (sample B). The basal spacing (d 002) of sample A was increased from 8.5764 Å to 17.18 Å upon intercalation of ETO. Further ETO loading was found to be 27.72% as estimated by UV-Vis spectrophotometer. The release profile of ETO from sample B in phosphate buffer saline (PBS) at pH 7.4 follows quasi-Fickian diffusion phenomenon in Korsmeyer-Peppas kinetics model. In vitro cell viability was undertaken using A549 (human lung adenocarcinoma) cell line in a dose dependent manner to determine the synergistic anti-cancer potential of sample B. It was observed that at an equivalent dose, the cancer cell viability was substantially reduced, in case of sample B compared to sample A and bare etoposide drug, respectively, which was further corroborated by cell proliferation/migration assay. The cellular uptake using A549 cell line showed an increasing trend with increasing time period (11.67% and 19.30% at 24 h and 72 h) respectively, confirmed by flow cytometry, exhibiting a substantial reduction of CAMKIIα protein for sample A and sample B to 110.52 pg/ ml and 95.14 pg/ml respectively. Results also showed significant down regulation of SOD gene activity. All the above results exhibited the synergistic anti-cancer potential of sample B for possible application in the management of non small cell lung carcinoma (NSCLC).
Surface and Coatings Technology, 2019
, An in vitro evaluation of the variation in surface characteristics of bioactive glass coated SS... more , An in vitro evaluation of the variation in surface characteristics of bioactive glass coated SS316L for load bearing application, Surface & Coatings Technology (2018),
Journal of Drug Delivery Science and Technology, 2019
The possibility of the use of small non coding RNAs as therapeutics in specifically targeting and... more The possibility of the use of small non coding RNAs as therapeutics in specifically targeting and treatment of damaged hard tissue are presently being explored largely and for the same, a good understanding of the underlying molecular mechanisms of osteoblast and osteoblast differentiation is necessary, towards better fixation of the implant. In the present communication, we made an attempt to incorporate a model non coding RNA viz. shRNA , in the form of shRNA-plasmid, in a porous bioactive coating of phosphate free bioactive glass (PFBG) on surgical grade SS316L implant material, used as reservoir/carrier of the biomolecule and evaluated its time dependent inhibitory action on the osteoclastogenic cytokine, tumour necrosis factor alpha (TNFα), that in turn aids in growth and differentiation of osteoblast cells, for faster remodeling, repair and healing of the damaged hard tissue at the implanted site. Upon treatment with the shRNA-plasmid, results exhibited significant downregulation (approximately 12-fold) of the TNFα, followed by upregulation of alkaline phosphatase (approximately 4-fold), corresponding to osteoblast formation, on the contrary, confirming a pivotal role of the same in osteoblast formation. The FACS data showed significant cellular uptake (17%) of the shRNAplasmid into osteoclast cells, that was in turn corroborated by confocal imaging.
Journal of Drug Delivery Science and Technology, 2019
Intercalation of shRNA-plasmid in Mg-Al layered double hydroxide nanoparticles and its cellular i... more Intercalation of shRNA-plasmid in Mg-Al layered double hydroxide nanoparticles and its cellular internalization for possible treatment of neurodegenerative diseases,
Applied Clay Science, 2019
In continuation with our previous report w.r.t the synthesis of phase pure and mixed phase calciu... more In continuation with our previous report w.r.t the synthesis of phase pure and mixed phase calcium aluminum layered double hydroxide (CaAl-LDH) and its subsequent evaluation with regard to anticancer property, the present communication demonstrates the intercalation of plasmid encoded short hairpin RNA (shRNA) into the interlayer space of CaAl-LDH nanoparticle (precipitated both at pHs 8.5 and 12.5, designated as sample A and B respectively) by a simple technique to obtain the requisite loading, followed by evaluation of the cellular uptake and localization. The presence of interlayer carbonate anion (CO 3 2−) as an impurity, were quantified both in the samples A and B, using ion chromatography, of which, the later exhibited a higher value (6.10 ppm) which in turn had hindered the intercalation of the shRNA, largely. Further, the cellular uptake using colon cancer cell line (HCT 116) could be correlated, based on the above data, as, for sample A, a threefold higher value (9.34%) was found in a period of 72 h, compared to sample B (3.54%), without using any transfection reagent. This observation was corroborated using confocal imaging to understand the internalization at the cellular level.
Current Topics in Medicinal Chemistry, 2016
HOSSANURUBLICATION, 2022
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Papers by HOSSANUR MONDAL