Papers by Tássia de Oliveira
Revista Ártemis, 2018
Partimos do pressuposto de que a voz lírica nos poemas colasantianos evidenciam as relações e as ... more Partimos do pressuposto de que a voz lírica nos poemas colasantianos evidenciam as relações e as tensões que se estabelecem no campo cultural marcado por um sistema de gênero que prioriza o masculino, onde um eu lírico se apresenta pela perspectiva das mulheres. Elegemos o corpo como categoria de análise e a crítica feminista como nossa principal fundamentação teórica. Nossa metodologia consiste na leitura analítica de poemas selecionados, que buscam evidenciar o corpo feminino, erotizado ou não, reconhecidamente um tema recorrente na poética colasantiana, também apontando a poesia lírica de Colasanti como uma forma de ruptura com a interdição sobre a interlocução erótica e os tabus que rondam os corpos das mulheres. Concluímos que a autora dá voz aos desejos de tantas mulheres que querem liberdade para seus corpos e também para si mesmas.
Sensors and Actuators B: Chemical, 2021
The outbreak of the COVID-19 pandemic, caused by Severe Acute Respiratory Syndrome of Coronavirus... more The outbreak of the COVID-19 pandemic, caused by Severe Acute Respiratory Syndrome of Coronavirus 2 (SARS-CoV-2), has fueled the search for diagnostic tests aiming at the control and reduction of the viral transmission. The main technique used for diagnosing the Coronavirus disease (COVID-19) is the reverse transcription-polymerase chain reaction (RT-PCR) technique. However, considering the high number of cases and the underlying limitations of the RT-PCR technique, especially with regard to accessibility and cost of the test, one does not need to overemphasize the need to develop new and less expensive testing techniques that can aid the early diagnosis of the disease. With that in mind, we developed an ultrasensitive magneto-assay using magnetic beads and gold nanoparticles conjugated to human angiotensin-converting enzyme 2 (ACE2) peptide (Gln24-Gln42) for the capturing and detection of SARS-CoV-2 Spike protein in human saliva. The technique applied involved the use of a disposable electrochemical device containing eight screen-printed carbon electrodes which allow the simultaneous analysis of eight samples. The magneto-assay exhibited an ultra-low limit of detection of 0.35 ag mL−1 for the detection of SARS-CoV-2 Spike protein in saliva. The magneto-assay was tested in saliva samples from healthy and SARS-CoV-2-infected individuals. In terms of efficiency, the proposed technique – which presented a sensitivity of 100.0% and specificity of 93.7% for SARS-CoV-2 Spike protein-exhibited great similarity with the RT-PCR technique. The results obtained point to the application potential of this simple, low-cost magneto-assay for saliva-based point-of-care COVID-19 diagnosis.
Estudos sobre a mensuracao da segregacao residencial e a identificacao da sua configuracao espaci... more Estudos sobre a mensuracao da segregacao residencial e a identificacao da sua configuracao espacial sao bastante escassos na literatura do pais, mais ainda para a cidade do Recife. O presente estudo objetiva contribuir para a analise da segregacao residencial nas cidades do Brasil, tendo como escopo a identificacao desse fenomeno na cidade do Recife para os anos de 2000 e 2010. Para tanto, sao utilizados dados dos setores censitarios dos Censos Demograficos de 2000 e 2010 do IBGE para os grupos populacionais descritos pela variavel rendimento dos responsaveis. A partir do computo das medidas sinteticas espaciais de segregacao e da espacializacao dos indices locais, as evidencias apontam para padroes de macrossegregacao na cidade. Especificamente, para os responsaveis com rendimento superior a 10 salarios minimos, ha um claro padrao de concentracao espacial desses grupos nas regioes que apresentam amenidades locais: Rio Capibaribe, Praia de Boa Viagem e parques da cidade. Alem disso,...
A presente pesquisa enfoca a producao poetica da escritora brasileira contemporânea Marina Colasa... more A presente pesquisa enfoca a producao poetica da escritora brasileira contemporânea Marina Colasanti, sendo o corpus de analise composto por poemas de seus quatro livros de poesia, (1993), (1998), (2005) e (2009). No âmbito mais amplo, partimos do pressuposto de que a producao literaria de autoria feminina foi, ao longo de seculos, parcialmente silenciada pela tradicao cultural patriarcal, o que justifica nossa proposta de apresentar um trabalho que se alie com os movimentos de reconhecimento da producao de escritoras contemporâneas no contexto nacional, vinculados aos estudos sobre mulher e literatura, proposta que percebemos como ainda necessaria, apesar dos avancos alcancados. Adotamos o entendimento de que a voz lirica nos poemas colasantianos selecionados evidenciam as relacoes e as tensoes que se estabelecem no campo cultural marcado por um sistema de genero que prioriza o masculino, onde um eu lirico se apresenta pela perspectiva das mulheres. Elegemos, nesse sentido, o corpo...
Briefly, the patterns of the electrodes were designed using the Silhouette Studio v3.8 software. ... more Briefly, the patterns of the electrodes were designed using the Silhouette Studio v3.8 software. Using a vinyl adhesive, the patterns were cut using the cutter printer (Silhouette Cameo, Brazil) and the unwanted adhesive parts were removed using tweezers, forming a mask (Fig. S1, step a). The vinyl mask was attached to a polyester sheet and carbon ink was spread over it, with the aid of a squeegee (Fig. S1, step b). Then, * *
Analytical and Bioanalytical Chemistry, 2019
The evaluation of interaction between small molecules and protein is an important step in the dis... more The evaluation of interaction between small molecules and protein is an important step in the discovery of new drugs and to study complex biological systems. In this work, an alternative method was presented to evaluate small-molecule-protein interaction by using ligand capture by protein-coated magnetic particles (MPs) and disposable electrochemical cells. The interaction study was conducted using [10]-gingerol from ginger rhizome and a transmembrane protein αVβ3 integrin. Initially, the electrochemical behavior of the natural compound [10]-gingerol was evaluated with the disposable carbon-based electrodes and presented an irreversible oxidation process controlled by diffusion. The analytical curve for [10]-gingerol was obtained in the range of 1.0 to 20.0 μmol L −1 , with limit of detection of 0.26 μmol L −1. Then MPs coated with αVβ3 integrin were incubated with standard solutions and extracts of ginger rhizome for [10]-gingerol capture and separation. The bioconjugate obtained was dropped to the disposable electrochemical cells, keeping a permanent magnet behind the working electrode, and the binding process was evaluated by the electrochemical detection of [10]-gingerol. The assay method proposed was also employed to calculate the [10]-gingerol-αVβ3 integrin association constant, which was calculated as 4.3 × 10 7 M −1. The method proposed proved to be a good label-free alternative to ligand-protein interaction studies.
Anais da Academia Brasileira de Ciências, 2018
Asian soybean rust (Phakopsora pachyrhizi-ASR) is one of the major diseases that occur in soybean... more Asian soybean rust (Phakopsora pachyrhizi-ASR) is one of the major diseases that occur in soybean and causes great damage to commercial crops. Therefore, the goal of this work was to investigate the relationship between biochemical and photosyntetic parameters in soybean with ASR. Two experiments were performed in a randomized complete block with three treatments (water, Tween 20, and methyl jasmonate). The evaluated traits were: severity, chlorophyll pools, concentration of phenolic compounds content, enzyme activity, and photosyntetic parameters. Based on Pearson correlation, correlation network and path analysis it was verified that the severity had high correlation with almost all traits evaluated meanwhile photosynthesis is weakly related with almost all traits. Therefore, the occurrence of ASR affects directly the traits related to enzymatic activity and phenolic compounds content in soybean plants inoculated with ASR. The management of ASR is important to keep in normal levels the rates of photosynthesis carried out by the plant, and thus not affect the yield. Besides that, understanding the biochemical mechanisms and ecophysiological responses that occur during the soybean-P. pachyrhizi interaction has a great importance for breeding programs, as it will help for obtaining resistant cultivars or efficient methods in fungus control.
Talanta, 2018
This paper describes a simple, low-cost, and highly flexible rapid prototyping method to construc... more This paper describes a simple, low-cost, and highly flexible rapid prototyping method to construct electrochemical paper-based analytical device (ePAD) for multiplexed analyte determinations. The ePAD was composed of two electrochemical cell (EC) compartments, separated by hydrophobic barriers of wax, and screen-printed electrodes (SPEs) deposited directly over the surface of the filter paper. The ePAD was entirely constructed using an inexpensive craft cutter printer with no needed of a wax printer. The rapid prototyping method involves two steps: the deposition of the SPEs and the creation of the wax barriers. In this case, the SPEs were screen-printed on filter paper by using adhesive tape as mask by cutting the electrodes pattern with the cutter printer. Following, the wax barriers were created using stamps made of filter paper also cut with the printer and impregnated with wax. In the ePAD, each ECs containing an array of 4-working electrodes, allowing up to 4 replicates in a single measurement. Both ECs shared one counter
PloS one, 2018
Asian rust affects the physiology of soybean plants and causes losses in yield. Repeatability coe... more Asian rust affects the physiology of soybean plants and causes losses in yield. Repeatability coefficients may help breeders to know how many measurements are needed to obtain a suitable reliability for a target trait. Therefore, the objectives of this study were to determine the repeatability coefficients of 14 traits in soybean plants inoculated with Phakopsora pachyrhizi and to establish the minimum number of measurements needed to predict the breeding value with high accuracy. Experiments were performed in a 3x2 factorial arrangement with three treatments and two inoculations in a random block design. Repeatability coefficients, coefficients of determination and number of measurements needed to obtain a certain reliability were estimated using ANOVA, principal component analysis based on the covariance matrix and the correlation matrix, structural analysis and mixed model. It was observed that the principal component analysis based on the covariance matrix out-performed other me...
Sensors and Actuators B: Chemical, 2017
Highlights The construction of a simple and low-cost 8-electrode fully disposable microfluidic ... more Highlights The construction of a simple and low-cost 8-electrode fully disposable microfluidic device was demonstrated. The device was successfully applied for ultrasensitive detection of Salmonella typhimurium. A single microfluidic device allows the simultaneous measurement of eight samples by magneto-immunoassay The magneto-immunoassay provides high sensitivity and fast response for Salmonella detection in milk samples.
Biosensors and Bioelectronics, 2015
In this work, a biosensor was constructed by physical adsorption of the isolated endophytic fungu... more In this work, a biosensor was constructed by physical adsorption of the isolated endophytic fungus Eupenicillium shearii FREI-39 esterase on halloysite, using graphite powder, multi-walled carbon nanotubes and mineral oil for the determination of carbofuran pesticide by inhibition of the esterase using square-wave voltammetry (SWV). Specific esterase activities were determined each 2 days over a period of 15 days of growth in four different inoculation media. The highest specific activity was found on 6th day, with 33.08 U on PDA broth. The best performance of the proposed biosensor was obtained using 0.5 U esterase activity. The carbofuran concentration response was linear in the range from 5.0 to 100.0 mg L À 1 (r ¼0.9986) with detection and quantification limits of 1.69 mg L À 1 and 5.13 mg L À 1 , respectively. A recovery study of carbofuran in spiked water samples showed values ranging from 103.8 7 6.7% to 106.77 9.7%. The biosensor showed good repeatability and reproducibility and remained stable for a period of 20 weeks. The determination of carbofuran in spiked water samples using the proposed biosensor was satisfactory when compared to the chromatographic reference method. The results showed no significant difference at the 95% confidence level with t-test statistics. The application of enzymes from endophytic fungi in constructing biosensors broadens the biotechnological importance of these microorganisms.
The Analyst, 2014
Sepiolite clay mineral was used as a support for the immobilisation of the peroxidase enzyme from... more Sepiolite clay mineral was used as a support for the immobilisation of the peroxidase enzyme from ingá-cipó (Inga edulis Mart.) and was used with graphite powder, multi-walled carbon nanotubes (CNTs), mineral oil, and nafion 0.5% (v/v) in the development of a new biosensor for the determination of the antioxidant tert-butylhydroquinone (TBHQ) by square-wave voltammetry (SWV). For the optimisation and application of the biosensor, several parameters were investigated to determine the optimum experimental conditions using SWV. The best performance was obtained using a 0.1 mol L(-1) phosphate buffer solution (pH 7.0), 4.0 × 10(-4) mol L(-1) hydrogen peroxide, a frequency of 50 Hz, a pulse amplitude of 60 mV, and a scan increment of 6 mV. The biosensor showed good repeatability and reproducibility and remained stable for a period of 20 weeks. The analytical curve revealed a linear response range of 1.65 to 9.82 mg L(-1) (r = 0.994) with detection and quantification limits of 0.41 and 1.25 mg L(-1). A recovery study of TBHQ in salad dressing samples yielded values from 99.6-104.8%. The proposed biosensor was successfully used for the determination of TBHQ in commercial salad dressing samples, giving a relative error of 5.4% in relation to the comparative method (chromatographic).
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Papers by Tássia de Oliveira