Introduction. The live vaccines against anthrax have a wide application in Ukrainian husbandry. W... more Introduction. The live vaccines against anthrax have a wide application in Ukrainian husbandry. We haven't enough knowledge to foresee all threats from vaccine strain burden on Ukrainian environment. We conducted the Bacillus spp. study in areas with different strategies of anthrax vaccination at regional porcine husbandries.
Objective-To study of the right and left rolling magnetic fields influence on CSF virus with Past... more Objective-To study of the right and left rolling magnetic fields influence on CSF virus with Pasteurella multocida physical interaction and virus survival under extreme temperature. Methods-We used Grabbina' device to asymmetric microgravity developments of virus-bacteria mixes (V mixes =5,0cm 3 ; field =0,2 mTl, photo 1) which consisted from 4,5 lg FFU 50/сm 3 of CSF virus ("ІЕCVМ-03" vaccine strain, CSFV) and the 5x10 5 BC 50/сm 3 of Pasteurella multocida "7" museum strain on buffered saline (pH 7,4; BS). Mixes in standard conditions were exposed by right (RMsamples, n=3) or left (LM-samples, n=3) rolling magnetic fields during 25 min. Control mixes (CM-samples, n=3) were exposed on switch off device. After microgravity expositions all mixes were rinsed (1x, BS) by low speed centrifugation, the settlements of RM-samples divided on 3 part -1st parts controlled immediately altogether with LM-and CM-specimens. 2nd and 3rd parts controlled after exposition under 40 o C in 3 and 5 days. The virus quantity in all mixes' settlements was enumerated by titration methods: PK-15 cell in cultural plates inoculation (40min contact with samples dilutions, 5-7x washing) and 48hr-incubation in antibiotics presence, then monolayer was fixed and developed by standard procedure of indirect peroxidase labeled antibody (PLA) method. Titers calculated by Reed & Muench (1938). Results-There was found that CSFV presented only in bacterial settlements all RM-samples (n=3, photo 2). The bacterial settlements of LM-and CM-samples were virus-negative. The virus concentration in consortia with P. multocida contained 2,5-3,0 lg FFU 50/сm 3 at immediately after development (n=4, photo 3). On 3 rd and 5 th day after asymmetric field extreme temperature exposition (40 o C) bacterial settlements of RM-samples contained 1,5-2,0 lg FFU 50/сm 3 of CSFV. Free CSF virus was inactivated on 2 nd day after incubation under the same conditions completely. Conclusion-Right-directed rolling magnetic field promote the classical swine fever virus adsorption on Pasteurella multocida cells (n=3, P< 0,01). Adsorbed virus was resistant to extreme temperature significantly. This dates may explain the events which may be to come during natural CSF outbreaks.
УДК 616. 98:579.842.11 МОДИФІКОВАНІ ІНФЕКЦІЙНІ АГЕНТИ (М-АГЕНТИ) СВИНЕЙ ТА ЕМЕРДЖЕНЦІЯ ІНФЕКЦІЙНИ... more УДК 616. 98:579.842.11 МОДИФІКОВАНІ ІНФЕКЦІЙНІ АГЕНТИ (М-АГЕНТИ) СВИНЕЙ ТА ЕМЕРДЖЕНЦІЯ ІНФЕКЦІЙНИХ ХВОРОБ У СВИНАРСТВІ 1 доцент Бузун А.І. Національний науковий центр «Інститут експериментальної і клінічної ветеринарної медицини» Національної Академії Аграрних Наук України
Introduction. The live vaccines against anthrax have a wide application in Ukrainian husbandry. W... more Introduction. The live vaccines against anthrax have a wide application in Ukrainian husbandry. We haven't enough knowledge to foresee all threats from vaccine strain burden on Ukrainian environment. We conducted the Bacillus spp. study in areas with different strategies of anthrax vaccination at regional porcine husbandries.
Objective-To study of the right and left rolling magnetic fields influence on CSF virus with Past... more Objective-To study of the right and left rolling magnetic fields influence on CSF virus with Pasteurella multocida physical interaction and virus survival under extreme temperature. Methods-We used Grabbina' device to asymmetric microgravity developments of virus-bacteria mixes (V mixes =5,0cm 3 ; field =0,2 mTl, photo 1) which consisted from 4,5 lg FFU 50/сm 3 of CSF virus ("ІЕCVМ-03" vaccine strain, CSFV) and the 5x10 5 BC 50/сm 3 of Pasteurella multocida "7" museum strain on buffered saline (pH 7,4; BS). Mixes in standard conditions were exposed by right (RMsamples, n=3) or left (LM-samples, n=3) rolling magnetic fields during 25 min. Control mixes (CM-samples, n=3) were exposed on switch off device. After microgravity expositions all mixes were rinsed (1x, BS) by low speed centrifugation, the settlements of RM-samples divided on 3 part -1st parts controlled immediately altogether with LM-and CM-specimens. 2nd and 3rd parts controlled after exposition under 40 o C in 3 and 5 days. The virus quantity in all mixes' settlements was enumerated by titration methods: PK-15 cell in cultural plates inoculation (40min contact with samples dilutions, 5-7x washing) and 48hr-incubation in antibiotics presence, then monolayer was fixed and developed by standard procedure of indirect peroxidase labeled antibody (PLA) method. Titers calculated by Reed & Muench (1938). Results-There was found that CSFV presented only in bacterial settlements all RM-samples (n=3, photo 2). The bacterial settlements of LM-and CM-samples were virus-negative. The virus concentration in consortia with P. multocida contained 2,5-3,0 lg FFU 50/сm 3 at immediately after development (n=4, photo 3). On 3 rd and 5 th day after asymmetric field extreme temperature exposition (40 o C) bacterial settlements of RM-samples contained 1,5-2,0 lg FFU 50/сm 3 of CSFV. Free CSF virus was inactivated on 2 nd day after incubation under the same conditions completely. Conclusion-Right-directed rolling magnetic field promote the classical swine fever virus adsorption on Pasteurella multocida cells (n=3, P< 0,01). Adsorbed virus was resistant to extreme temperature significantly. This dates may explain the events which may be to come during natural CSF outbreaks.
УДК 616. 98:579.842.11 МОДИФІКОВАНІ ІНФЕКЦІЙНІ АГЕНТИ (М-АГЕНТИ) СВИНЕЙ ТА ЕМЕРДЖЕНЦІЯ ІНФЕКЦІЙНИ... more УДК 616. 98:579.842.11 МОДИФІКОВАНІ ІНФЕКЦІЙНІ АГЕНТИ (М-АГЕНТИ) СВИНЕЙ ТА ЕМЕРДЖЕНЦІЯ ІНФЕКЦІЙНИХ ХВОРОБ У СВИНАРСТВІ 1 доцент Бузун А.І. Національний науковий центр «Інститут експериментальної і клінічної ветеринарної медицини» Національної Академії Аграрних Наук України
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