Papers by mariana berechet
Leather and Footwear Journal
In this research, the aim was to obtain keratin hydrolysate from wool waste from sheep breeders a... more In this research, the aim was to obtain keratin hydrolysate from wool waste from sheep breeders and use it in technologies for dyeing bovine hides. The keratin hydrolysate (KerNa), obtained by alkaline hydrolysis with sodium hydroxide, was physico-chemically analyzed, determining the protein substance in the amount of 80.65%, highlighting the bands specific to peptides and compounds with sulfur by FTIR spectroscopy and particle size by DLS technique, obtaining majority populations at 161 nm and 615 nm. Bovine hides were treated with keratin hydrolysate, in different stages of the dyeing process, and an increase in the dyeing resistance to wet and dry rubbing and the dyeing resistance to water drops was obtained, as well as the improvement of the specific color parameters. Leathers dyed with the use of the KerNa additive showed an increase in the friction resistance of the dyeing (grade 5/5) and brighter colors according to ISO Brightness. Treatments based on protein-rich keratin hyd...
This work presents experiments in making biopolymer-based materials with smart functionalities fr... more This work presents experiments in making biopolymer-based materials with smart functionalities from renewable resources in the leather industry, applicable in various areas. Keratin hydrolysates were obtained by alkaline hydrolysis in the presence of NaOH and CaO. The physico-chemical characterization, DLS and FT-IR analysis of keratin hydrolysates highlighted the rich content in protein and total nitrogen. The recovery of wool by-products from the leather industry leads to less waste and helps prevent environmental pollution.
Proceedings of the 9th International Conference on Advanced Materials and Systems
The interaction of turmeric powder with five surfactants (isopropyl oleate, diester of sucrose, p... more The interaction of turmeric powder with five surfactants (isopropyl oleate, diester of sucrose, polymethylene-α, ω-bis (N, N-dialkyl-N-deoxy-d-glucitolammonium iodides, bis [2-butyl (sodium bis-thioacetate) sodium dicarboxylate 1,10 decanediyl] ester, demecarium bromide) and obtaining nanoemulsions, has been investigated by spectroscopy, dynamic light scattering, optical microscopy and microbiological tests. The modeling encapsulation of turmeric powder in nanoemulsions was carried out taking into account the following parameters: the concentration and type of surfactants, the ratio between turmeric and surfactant, micellar critical concentration, speed and time of stirring, temperature, pH, average diameter of particles, zeta potential, conductivity. The known antibacterial and anti-inflammatory properties of turmeric can be improved by dispersing it in nanoemulsions resulting in better functional efficacy. The specific factors in designing nanoemulsion systems that affect the chem...
Materials
Hydrolysed collagen obtained from bovine leather by-products were loaded with ginger essential oi... more Hydrolysed collagen obtained from bovine leather by-products were loaded with ginger essential oil and processed by the electrospinning technique for obtaining bioactive nanofibers. Particle size measurements of hydrolysed collagen, GC-MS analysis of ginger essential oil (EO), and structural and SEM examinations of collagen nanofibers loaded with ginger essential oil collected on waxed paper, cotton, and leather supports were performed. Antioxidant and antibacterial activities against Staphylococcus aureus and Escherichia coli and antifungal activity against Candida albicans were also determined. Data show that the hydrolysed collagen nanofibers loaded with ginger EO can be used in the medical, pharmaceutical, cosmetic, or niche fields.
Proceedings of the 9th International Conference on Advanced Materials and Systems, Nov 15, 2022
The amino acid composition in the hydrolysates of fish proves to be a most promising source of pr... more The amino acid composition in the hydrolysates of fish proves to be a most promising source of protein. Two extracts from fish by-products (P1, from the head and fins of sturgeon and P2, from the cartilage of sturgeon head and fins) were obtained as liquids that were dried at 40°C. Liquid extracts were characterized physico-chemically (dry matter 3.86% and 4.25%, protein content 1.44% and 3.25%), and particle size (247nm, 94% and 4148nm, 65% majority populations for P1 and P2) and zeta potential (-27.4mV and-15.8mV) were measured. The smaller particle size for the P1 extract led to its choice for treatments applied in the growth of corn seeds. Four samples of concentrations of 0.5%, 1%, and 1.5% and control concentrations were experimented, each on 25 corn seeds, observing the growth of plants over a period of 13 days. A 13% higher increase of the corn plants was obtained in the case of the sample treated with 1.5% fish protein extract, P1. Throughout the experiments, the P1 sample with 1.5% fish protein extract had higher increases than the control sample. These results suggest that extracts from the head by-products of sturgeon fish could be used in agriculture as a nutrient in the growth of corn plants. Keywords: protein extracts from fish head, majority populations of particle sizes, nutrients in the growth of corn seed
Proceedings of the 9th International Conference on Advanced Materials and Systems, Nov 15, 2022
The aim of the paper is to obtain new byproducts based on surfactants (gemini-polymethylene-α, ω-... more The aim of the paper is to obtain new byproducts based on surfactants (gemini-polymethylene-α, ω-bis (N, N-dialkyl-N-deoxy-d-glucitolammonium iodides or bolaform-demecarium bromide) and protein hydrolysates (keratin and collagen) with micro and macro nutrients for applications in agriculture. A method was developed to include micro and macronutrients in keratin and collagen hydrolysates, in order to obtain new byproducts-bioemulsions (stable because of surfactants), with final goal of application as a new class of root fertilizers for cereals (e.g., corn). The newly obtained byproducts (bioemulsions based on surfactants) were characterized by: dynamic light scattering measurements, contact angle, optical microscopy and microbiological tests against fungal attack of Fusarium spp. and Botrytis cinerea. Better results were obtained for gemini surfactant based on sugar-polymethylene-α, ω-bis (N, N-dialkyl-N-deoxy-d-glucitolammonium iodides) due to the properties such as: biodegradability, nontoxicity and adherence to surfaces. The new fertilizer created in this research-bioemulsions based on surfactants, can support the general structure of the grains as well as the chlorophyll content, increasing the growth yield. The fertilizer is indicated for any type of crops and soils, with recommended use as additional fertilizer for plants (cereals) in the vegetation and growth phases, with a maximum need for nutrients. Keywords: hydrolysates (keratin and collagen) with micro and macro nutrients; byproductsbioemulsions based on surfactants; new class of root fertilizers in cereals
Proceedings of the 9th International Conference on Advanced Materials and Systems
Ocimum basilicum L. is an aromatic plant in the family Lamiaceae with bioactive properties used s... more Ocimum basilicum L. is an aromatic plant in the family Lamiaceae with bioactive properties used since ancient times in traditional medicine. The active ingredients of basil essential oil can be used in perfumes, pharmaceuticals, medicine, cosmetics or spices. In this study, the essential oil of basil was obtained by hydrodistillation in the Clevenger continuous extractor. It was characterized by GC-MS and 53 constituent compounds were identified. The majority compounds were highlighted: linalool, 64,569%, p-allyl anisol, 5,163%, Eucalyptol, 3,745%, α-Cadinene, 3,510%. Kovats indices were calculated and FT-IR analysis was performed to confirm the specific constituent compounds. The essential oil of basil was microbiologically analyzed against Escherichia coli (ATCC 10536) Gram-negative bacteria and against Staphylococcus aureus (ATCC 6538) Gram-positive bacteria by diffusometric working method. Antibacterial activity was determined by measuring the diameter of the inhibition zone aro...
The International Conference on Advanced Materials and Systems, 2016
Keratin is a biopolymer with numerous functional properties for the production of biomaterials wi... more Keratin is a biopolymer with numerous functional properties for the production of biomaterials with applications such as: additives for cosmetics, biostimulator for growth and nutrition in agriculture, ecological treatments in reconstruction and protection of leather and furs, as alternative to pollutant chemical compounds. In most of the applications, in either large or niche industries, keratin is used as gels, films, nano-or micro-particles. This study presents the possibilities of using thermal and enzymatic processes of leather industry by-products to obtain keratin extracts. Keratin extracts were characterized by chemical and instrumental analyses: gravimetric, volumetric, potentiometry, Polyacrylamide Gel Electrophoresis, Infrared Spectroscopy, High-Performance Liquid Chromatography, Dynamic Light Scattering. Analytical investigation has shown that the keratin extracts obtained as polydispersions with 5% dry matter have 14% total nitrogen and contain 50% small and medium sized particles (10-500 nm range), such as free amino acids and oligopeptides and 50% larger sized particles (500-5560 nm ranges) such as polypeptides. The IR spectrum of keratin extract is similar to the IR spectrum of collagen from leather.
Bioinorganic Chemistry and Applications, 2014
This paper presents the improvement of the antimicrobial character of woven fabrics based on cott... more This paper presents the improvement of the antimicrobial character of woven fabrics based on cotton. The woven fabrics were cleaned in oxygen plasma and treated by padding with silver chloride and titanium dioxide particles. The existence of silver and titanium on woven fabrics was evidenced by electronic microscope images (SEM, EDAX) and by flame atomic absorption spectrophotometry. The antimicrobial tests were performed with two fungi:Candida albicansandTrichophyton interdigitale. The obtained antimicrobial effect was considerably higher compared to the raw fabrics. Treatment of dyed fabrics with a colloidal solution based on silver chloride and titanium dioxide particles does not considerably influence colour resistance of dyes.
Revista de Chimie -Bucharest- Original Edition-
ABSTRACT
TEXTEH Proceedings, 2021
One approach to develop innovative antimicrobial wound dressing materials is to use natural polym... more One approach to develop innovative antimicrobial wound dressing materials is to use natural polymers loaded with antimicrobial agents. The valorisation of animal proteins as biomaterials with antimicrobial properties is a new concern for development of wound healing. Plant esssential oils (EO) also indicate a potential approach for new wound dressing materials able to replace the synthetic antymicrobial agents. In this paper, plant-polymeric film was prepared by casting film-forming emulsion based on lemongrass (Cymbopogon flexuosus) essential oil/Tween 80 dispersed into rabbit collagen glue hydrolysate– chitosan biomaterial. The effect of biomaterial film composition on Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 10536 standard bacteria, and Candida albicans ATCC 10231 pathogenic fungus was studied according to European Pharmacopoeia 10/2020 as compared with biomaterial film without essential oil. The in vitro antibacterial tests against three bacterial strains showed...
ELENA MANAILA1, MARIANA DANIELA BERECHET2, MARIA DANIELA STELESCU2, GABRIELA CRACIUN1*, DAN EDUAR... more ELENA MANAILA1, MARIANA DANIELA BERECHET2, MARIA DANIELA STELESCU2, GABRIELA CRACIUN1*, DAN EDUARD MIHAIESCU3, BOGDAN PURCAREANU3, IOAN CALINESCU3, ADRIAN FUDULU3, MIHAI RADU3 1 National Institute for Laser, Plasma and Radiation Physics, Electron Accelerators Laboratory, 409 Atomistilor Str., 077125, Magurele, Romania 2 National R&D Institute for Textile and Leather – Leather and Footwear Research Institute, 93 Ion Minulescu Str,031215, Bucharest, Romania 3 University Politehnica of Bucharest, Department of Bioresources and Polymer Science, 1-7 Polizu Str., 011061, Bucharest, Romania
This paper investigates the compatibilization action of maleated ethylenepropylene-diene terpolym... more This paper investigates the compatibilization action of maleated ethylenepropylene-diene terpolymer ionomer on polymeric composites based on high density polyethylene and cured rubber powder. Two types of cured rubber powder obtained from rubber wastes were used. Composites were developed using the melt mixing technique. Physical-mechanical characteristics of polymeric composites depend on the type and amount of components in blends. The optimal amount of compatibilization agent was selected for developed polymeric composites. The resulted polymer composites can be used in the manufacture of a large range of products, such as hoses, gaskets, shoe heels, joint packings, slab pavements in sports halls etc.
The action of zinc oxide, ionic/non-ionic plasticizers and fillers on the physicalmechanical char... more The action of zinc oxide, ionic/non-ionic plasticizers and fillers on the physicalmechanical characteristics of ionomers (ionic thermoplastic elastomers) based on maleinized ethylene propylene terpolymers was investigated. The blend characteristics are dependent on the levels and types of components of blend. The optimum compositions are selected according to the application areas of the concerned blend.
Agriculture and Food, 2018
The aim of this research is the development of biomaterials with controlled characteristics from ... more The aim of this research is the development of biomaterials with controlled characteristics from renewable resources generated by leather and fur industry and characterization of smart functionalities, applicable in agriculture. Keratin hydrolysates were obtained by calcium oxide hydrolysis (KerCa) in view of agriculture applications. The chemical analysis of keratin hydrolysate indicates a calcium (9.93%) and protein-rich (72.83%) composition with a long-term release of organic nitrogen which represents 12.96% of the dry substance product. The SDS-PAGE electrophoresis of KerCa highlights the characteristic bands of high molecular weight proteins (20-10 kDa) but also rich in low molecular weight peptides (6-9 kDa). Diffuse Light Scattering analysis shows the presence of particle size populations of 712 nm and of 141 nm. Experiments regarding the nutritional and biostimulation capacity on plant growth were performed with KerCa in 1:20 and 1.5:20 dilutions on lentil and wheat seeds (T...
Fur and leather have been among the first materials used for clothing and bodily decoration. It i... more Fur and leather have been among the first materials used for clothing and bodily decoration. It is known that Homo sapiens and Homo neanderthalensis used fur clothing. Even though the invention of inexpensive synthetic textiles for insulating clothing led to fur clothing falling out of fashion, fur is still worn in most cool climates around the world such due to its superior warmth and durability. In addition, a huge number of furs exists in the ethnography and anthropology museums around the world. The storage and conservation of furs, old and modern, is still challenging for both conservators and population because most commercial products are highly toxic for humans and environment. We therefore tried to control or limit the damage caused by external factors and insects by using green finishing and maintenance treatments. It is known that essential oils, known for their special perfume, can be used to repel insects. Mint, cedar, lavender oils were hence tested to treat sheepskin ...
In this study, sodium alginate film (Alg) was coated with electrospun collagen glue (Col) extract... more In this study, sodium alginate film (Alg) was coated with electrospun collagen glue (Col) extracted from rabbit skin waste, loaded with different commercial antimicrobial agents (chitosan, AG425K and ZnONPs) and investigated in terms of morphological, structural and biological properties. The coated nanostructures were characterized using scanning electron microscopy coupled with the energy-dispersive X-ray (SEM/EDS), Attenuated Total Reflectance Fourier-Transform Infrared spectroscopy (ATR FT-IR), and Atomic Force Microscopy (AFM) tests. The cytotoxicity was investigated on murine L929 fibroblasts using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide salt (MTT) and lactate dehydrogenase (LDH) assays. Microbiological tests were performed against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Candida albicans ATCC 27853 standard strains. In vitro cell culture tests showed a good cytocompatibility of the coated nanostr...
MARIANA DANIELA BERECHET1, MARIA DANIELA STELESCU1*, ELENA MANAILA2, GABRIELA CRACIUN2 1National ... more MARIANA DANIELA BERECHET1, MARIA DANIELA STELESCU1*, ELENA MANAILA2, GABRIELA CRACIUN2 1National Research and Development Institute for Textile and Leather-Leather and Footwear Research Institute, Rubber Research Department, 93 Ion Minulescu Str., 031215, Bucharest, Romania 2National Institute for Laser, Plasma and Radiation Physics, Electron Accelerators Laboratory, 409 Atomistilor Str., 077125, Magurele, Romania
Keratin is a fibrous protein abundant in nature, being the component of wool, hair, hooves, horns... more Keratin is a fibrous protein abundant in nature, being the component of wool, hair, hooves, horns, feathers, and claws. Keratin is one of the most valuable natural biopolymers due to its chemical versatility and biological performance. At the molecular level, keratin is distinguished from other biopolymers by its high concentration of cysteine-containing sulfur. Two keratin hydrolysate batches were obtained in alkaline medium, at a constant concentration of 8% NaOH and 75°C (KerNa875), 85°C (KerNa885), and 95°C (KerNa895), and at a fixed temperature of 99°C and different concentrations of NaOH, i.e. 3% (KerNa399), 5% (KerNa599), and 8% (KerNa899), respectively. Physical-chemical analyses showed that the protein content ranging between 83.60% for KerNa875 and 88.88% for KerNa399, while the total nitrogen was found 13.83% and 14.67% in the case of KerNa875 and KerNa399, respectively. Dynamic light scattering analysis showed that the particle sizes decreased with the increased concentr...
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Papers by mariana berechet