Papers by ashutosh paliwal
The Journal of General and Applied Microbiology, 2018
Numerous microbes reside in the rhizosphere having plant growth promoting activity, 43 and enhanc... more Numerous microbes reside in the rhizosphere having plant growth promoting activity, 43 and enhancing the property by increasing plant yield. Plant growth promoting 44 rhizobacteria (PGPR) has gradually increased in agriculture and offers an attractive 45 way to replace chemical fertilizers, pesticides and supplements. Soil was collected from 46 the rhizosphere of an agricultural farm and the psychrotrophic bacterial strains STA 3 47 (KY888133) and RM 2 (KY888134) were successfully isolated, and screened on the 48 basis of phosphate solubilization. Further characterization was carried out by 49 morphological, biochemical, and 16S rDNA characterization methods. The unique 50 nature of psychrotrophic Pentoea ananatis and a suitable combination with 51 Pseudomonas fluorescens regarding plant growth promotion activity has not been 52 studied before to our knowledge. An assessment of various parameters of plant growth 53 promoting activity, such as IAA, phosphate solubilization, bio-control activity, HCN 54 and siderophore production, has been carried out. Both strains were found to be 55 positive in various parameters except HCN and Biocontrol activity, which were 56 positive only for the strain RM 2 . Also, shelf life and efficacy was determined before 57 and after formulation. A great consistency was observed in all the cultures, even after 58 70 days of storage under bio-formulation at room temperature, while in the case of the 59 co-culture CPP-2, the cfu ml -1 was greater, followed by RM 2 and STA 3 . Moreover, the 60 growth indices of the pea plant were found to be better in the co-culture CPP-2 61 compared with individual strains, followed by RM 2 and STA 3 . Thus, the study suggests 62 that the co-culture CPP-2 has a great potential for plant growth promotion as compared 63 with individual strains followed by RM 2 and STA 3 .
Drug Resistance in Bacteria, Fungi, Malaria, and Cancer, 2017
Cancer is currently a major public health problem worldwide. Recent data estimates that approxima... more Cancer is currently a major public health problem worldwide. Recent data estimates that approximately 40 % of men and women will develop some form of cancer, at some point during their lifetime. Major challenges in treating cancer include delayed diagnosis, emergence of resistance toward existing drugs and metastasis, etc. In majority of advance-stage cancers, the conventional therapies are mostly ineffective. Targeted therapies raise new hope in several difficult-to-treat cases, but their application is restricted by variable efficacies of these drugs in different cancers (tissue backgrounds) and genetic backgrounds. Efflux of drugs, inactivation of drugs, modification of drug targets, inhibition of cell death, induction of epithelial–mesenchymal transition (EMT), and enhanced DNA damage repair activity are the most common mechanisms of drug resistance in cancer. Here, we present an account of these drug resistance mechanisms in cancer and recent breakthroughs in drug development, which have been successful in deceiving most of these mechanisms of drug resistance. Additionally, prognostic biomarkers and approaches for diagnosis of drug resistance have also been discussed.
Journal of clinical and experimental dentistry, 2017
Bacterial biofilms formed on the root canal wall are often difficult to remove. This study aimed ... more Bacterial biofilms formed on the root canal wall are often difficult to remove. This study aimed to evaluate the cytotoxic effect and antibacterial efficacy of chitosan when used as root canal irrigant against E. Faecalis and Candida albicans biofilm formed on tooth substrate. The present study evaluated antibacterial effect of 0.25% Chitosan, 0.5% Chitosan, 2% chlorhexidine and 3% sodium hypochlorite against Enterococcus faecalis and Candida Albicans. Agar-well diffusion methods, minimal inhibitory concentration tests and biofilm susceptibility assays were used to determine antibacterial activity. Teeth specimens were sectioned to obtain a standardized tooth length of 12mm. Specimens were inoculated with 10 mL of the freshly prepared E. Faecalis suspension and Candida albicans for 4 weeks. The specimens were then instrumented with ProTaper rotary files F3 size. After irrigation with test solution, three sterile paper points were placed into one canal, left for 60 s and transferred ...
Naturally Occurring Chemicals Against Alzheimer's Disease
Compatible Solutes Engineering for Crop Plants Facing Climate Change
Compatible Solutes Engineering for Crop Plants Facing Climate Change
SOJ Microbiology & Infectious Diseases
Polycyclic Aromatic Hydrocarbons (PAH) are ubiquitous contaminants in environments, are generated... more Polycyclic Aromatic Hydrocarbons (PAH) are ubiquitous contaminants in environments, are generated as a by-product of incomplete combustion of organic substances. PAH continuously increases and accumulate in surrounding finally affect the environment as well as crop production. Thus, appropriate treatment is required to reduce the concentration and toxicity of these substances. Bioremediation, an effective method, uses the ability of an organism to reduce the concentration of PAH to an acceptable level. This study investigated the ability of PAHs (fluorene) degrading as well plant growth promoting activity by three bacterial strains (MHR 4 , MHR 2 and MB 2) isolated from crude oil polluted soil near fuel filling stations from Haldwani and Bhowali in Uttarakhand region. These strains showed considerable growth over fluorene, as the sole carbon source with 100-500 ppm concentration in Mineral Salt Medium (MSM) agar plates after 24 h. Although, all the strains have potential towards plant growth promoting activity. Comparative study of fluorene degradation was found prominent, 81.2% in strain MHR 4 followed by strain MB 2 , 55% and strain MHR 2 , 37.5%, likewise, the efficiencies of various PGP activities in strain MHR 4 were detected quite high. This was convincing enough to investigate the strain MHR 4 for their molecular identification and to check their efficiency in pot trail experiment. Therefore, the efficient strain MHR 4 was identified on the basis of 16S rDNA sequencing and confirmed as Enterobacter sp. The multi traits strain effectively to remove fluorene both from the oil contaminated environment as well as to eliminate the chemical fertilizer by providing diverse PGP activity, is a novel achievement and suggests that Enterobacter sp. MHR 4 have extremely useful for a biotechnological process involving fluorene degradation and plant growth promotion. The unique nature of fluorene degradation as well as plant growth promotion activity has not yet been studied in the same bacterial strain to our knowledge.
Microbial Technology for Sustainable Environment
Treating Endocrine and Metabolic Disorders With Herbal Medicines
Human life is surrounded and dependent on its environment. Human civilization is nurtured by natu... more Human life is surrounded and dependent on its environment. Human civilization is nurtured by nature as it provides raw materials that are used in the manufacturing of various essential products like medicine, food items, etc. Not only developing countries but developed countries also depend on herbal-based medications. Cancer is a global health burden. Epithelial-mesenchymal-transition (EMT) plays a key role in cancer progression and is also stimulated by different extracellular signals and could be regulated at different levels. Conventional therapies exhibit a cytotoxic effect, which encourages the development of a new approach that could be used with synthetic drugs. Phytotherapy emerged as an effective weapon against cancer. Herbal drugs directly target different signaling pathways that promote EMT and eventually lead to cancer.
Research Anthology on Food Waste Reduction and Alternative Diets for Food and Nutrition Security
Food wastage is a huge crisis arising in today's world. An extensive amount of waste generati... more Food wastage is a huge crisis arising in today's world. An extensive amount of waste generation has become a serious concern of our society in the past years that affects developing and developed countries equally, and according to the Food and Agriculture Organization (FAO), as much as one-third of the food intentionally grown for human consumption is never consumed and is therefore wasted, with significant environmental, social, and economic ramifications. By wasting food, we also waste the time and energy that we have used to produce the food and as well our natural resources and the limited available agricultural land will be used up which could be handled in a much better and sustainable way. Additionally, waste has a strong financial impact and affects the environment including the overall greenhouse gas emission. In an increasingly resource-constrained world, it is imperative to reduce the high environmental, social, and economic impacts associated with this type of waste.
PLOS ONE
Bacterial Leaf Blight (BLB) disease is an extremely ruinous disease in rice, caused by Xanthomona... more Bacterial Leaf Blight (BLB) disease is an extremely ruinous disease in rice, caused by Xanthomonas oryzae pv. oryzae (Xoo). Although various chemicals are available to manage BLB, they are toxic to the environment as well as humans. Hence there is a need to develop new pesticides as alternatives to hazardous chemicals. Therefore, a study was carried out to discover new potent natural pesticides against Xoo from different solvent extracts of Vernonia cinerea. Among all the fractions, the methanolic extract showed the highest inhibition zone. Further, to gain mechanistic insight of inhibitory action, 40 molecules of methanolic extracts were subjected for in silico study against two enzymes D-alanine—D-alanine ligase (Ddl) and Peptide deformylase (PDF). In silico study showed Rutin and Methanone, [1,4-dimethyl-7-(1- methylethyl)-2- azulenyl]phenyl have a good binding affinity with Ddl while Phenol, 2,4-bis(1-phenylethyl)- and 1,2-Benzenedicarboxylic acid, diisooctyl ester showed an exc...
Pathogenesis, Treatment and Prevention of Leishmaniasis
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Papers by ashutosh paliwal