Papers by Yunita Armiyanti
Abstrak: Tuberkulosis (TB) menjadi masalah kesehatan utama baik lingkup global maupun nasional. T... more Abstrak: Tuberkulosis (TB) menjadi masalah kesehatan utama baik lingkup global maupun nasional. Tuberkulosis menyebabkan status gizi kurang baik. Status gizi yang kurang baik dapat disebabkan oleh infeksi kronis, salah satunya infeksi cacing usus terutama Soil-Transmitted Helminths (STH). Ko-infeksi STH pada pasien TB selain mempengaruhi status gizi, juga akan mempengaruhi respon imun pasien. Respon imun pada pasien ko-infeksi STH akan menghambat respon imun Th 1 dalam melawan TB serta akan didapatkan peningkatan eosinofil (eosinofilia). Tujuan utama dalam penelitian ini adalah untuk mengetahui jumlah eosinofil pada pasien tuberkulosis terhadap ko-infeksi STH di Kecamatan Kalisat, Kabupaten Jember. Jenis penelitian ini adalah penelitian observasional dengan desain analitik cross sectional. Populasi yang digunakan adalah pasien TB di Kecamatan Kalisat dalam periode waktu September – Desember 2019. Data yang digunakan dalam penelitian ini adalah data primer berupa hasil pemeriksaan da...
Background: Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) is an important protein... more Background: Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) is an important protein responsible for the pathogenesis of severe malaria, including cerebral malaria. The protein is highly diverse. The CIDR1α-PfEMP1 binds endothelial protein receptor (EPCR) and may associated with the brain swelling in childhood malaria. Objective: To analyze the CIDR1α-PfEMP1 from Indonesian isolate and determine its association with cerebral malaria outcome. Methods: Fifteen blood samples of clinically mild to severe malaria-patient were collected for DNA extraction. Malaria diagnosis was conducted microscopically by Giemsa-stained thin blood smear. The CIDR1α domain was amplified by PCR using specific primer and PCR product was sequenced. The nucleotide sequences were analyzed by NCBI blast, DNASIS MAX 3 and translated into amino acid sequences using Expasy Translation Tool. Results: One out of fifteen samples was severe malaria case and infected with P. falciparum, the rest were clin...
Soil-Transmitted Helmithiasis is an infection caused by intestinal nematode worms which in the pr... more Soil-Transmitted Helmithiasis is an infection caused by intestinal nematode worms which in the process of transmission requires soil as media to fullfil its life cycle from non-infective forms to infective forms. The number of infections is influenced by personal hygiene. Personal hygiene consists of the habit of washing hands, cutting nails, eating, defecating and ownership of latrines, and the use of personal protective equipment. This study used a cross sectional research design conducted at Widodaren Plantation with 68 people of samples. Stool examination was determined by kato-katz method, sedimentation, and floatation to detect the presence of Soil-Transmitted Helminth (STH) eggs, while personal hygiene was assessed using questionnaires. Questionnaire and stool examination datas were processed using SPSS data analysis with chi-square method. The results of this study found 26 respondents who were positive for soil-trasmitted helminthiasis infection. The most frequent worm spec...
Scabies is caused by infestation and sensitization of Sarcoptes scabiei hominis varieties. Scabie... more Scabies is caused by infestation and sensitization of Sarcoptes scabiei hominis varieties. Scabies is easily transmitted to individuals who live in groups such as boarding schools. Knowledge of scabies that is lacking and low in individual hygiene among student causes a transmission to be faster and wider. The purpose of this study was to determine the relationship between the characteristics, level of knowledge and individual hygiene of the student with the occurrence of scabies at the Miftahul Ulum Islamic Boarding School in Kalisat District, Jember Regency. This study used an observational analytical method with cross-sectional. Of the population of 358 students, 229 students were willing to become respondents. The results showed that 105 students (45.9%) suffered from scabies. The chi-square test results showed the characteristics of respondents such as gender, pruritus complaints, pruritus onset when entering the hut, treatment history was associated with the incidence of scabi...
Jurnal ILMU DASAR
Ascaris lumbricoides is nematode which often infest human in the world. The infestation of A. lum... more Ascaris lumbricoides is nematode which often infest human in the world. The infestation of A. lumbricoides in the human will trigger the formation of Th2 immune responses through increased interleukin-4 released by innate immune cells. Th2 immune response generates B lymphocyte cells to produce IgE antibodies to eliminate A. lumbricoides. Therefore, IL-4 is a marker of Th2 immune response. The purpose of this study was to determine the prevalence of ascariasis and an increase of IL-4 in planters infested with A. lumbricoides as a marker of Th2 immune response. Our study was observational analytic with cross sectional design. 230 planters who were willing to be involved in the study gave stool samples for the diagnosis of ascariasis. 20 people who were positive for ascariasis and 20 people who were negative selected randomly for blood plasma samples. IL-4 levels in blood plasma were measured using ELISA. Differences in IL4 levels between positive and negative ascariasis were analyzed...
MNJ (Malang Neurology Journal)
Background: Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) is an important protein... more Background: Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) is an important protein responsible for the pathogenesis of severe malaria, including cerebral malaria. The protein is highly diverse. The CIDR1α-PfEMP1 binds endothelial protein receptor (EPCR) and may associated with the brain swelling in childhood malaria. Objective: To analyze the CIDR1α-PfEMP1 from Indonesian isolate and determine its association with cerebral malaria outcome. Methods: Fifteen blood samples of clinically mild to severe malaria-patient were collected for DNA extraction. Malaria diagnosis was conducted microscopically by Giemsa-stained thin blood smear. The CIDR1α domain was amplified by PCR using specific primer and PCR product was sequenced. The nucleotide sequences were analyzed by NCBI blast, DNASIS MAX 3 and translated into amino acid sequences using Expasy Translation Tool. Results: One out of fifteen samples was severe malaria case and infected with P. falciparum, the rest were clini...
Journal of Health Sciences
Abstrak: Tuberkulosis (TB) menjadi masalah kesehatan utama baik lingkup global maupun nasional. T... more Abstrak: Tuberkulosis (TB) menjadi masalah kesehatan utama baik lingkup global maupun nasional. Tuberkulosis menyebabkan status gizi kurang baik. Status gizi yang kurang baik dapat disebabkan oleh infeksi kronis, salah satunya infeksi cacing usus terutama Soil-Transmitted Helminths (STH). Ko-infeksi STH pada pasien TB selain mempengaruhi status gizi, juga akan mempengaruhi respon imun pasien. Respon imun pada pasien ko-infeksi STH akan menghambat respon imun Th 1 dalam melawan TB serta akan didapatkan peningkatan eosinofil (eosinofilia). Tujuan utama dalam penelitian ini adalah untuk mengetahui jumlah eosinofil pada pasien tuberkulosis terhadap ko-infeksi STH di Kecamatan Kalisat, Kabupaten Jember. Jenis penelitian ini adalah penelitian observasional dengan desain analitik cross sectional. Populasi yang digunakan adalah pasien TB di Kecamatan Kalisat dalam periode waktu September – Desember 2019. Data yang digunakan dalam penelitian ini adalah data primer berupa hasil pemeriksaan da...
Jurnal Ners dan Kebidanan (Journal of Ners and Midwifery)
Tuberculosis (TB) is a contagious infectious disease caused by the bacteria Mycobacterium tubercu... more Tuberculosis (TB) is a contagious infectious disease caused by the bacteria Mycobacterium tuberculosis. More than 80% of TB cases attack the pulmonary organs and the rest are extra-pulmonary TB. Indonesia is one of the countries with the high number of tuberculosis cases besides India, China, the Philippines, and Pakistan. Aside from being a country with a high TB incidence, Indonesia is also an endemic country for helminth infections, especially Soil-Transmitted Helminths (STH). The high prevalence of STH infections in Indonesia allows STH coinfection in TB patients to cause decreasing immunity, thus affecting the outcome of TB infection. STH infection is very closely related to environmental sanitation. The purpose of this study was to identify and determine the association of environmental sanitation risk factors to the coinfection of Soil-Transmitted Helminths (STH) in tuberculosis patients in Panti District, Jember Regency. This type of research was observational research with ...
NurseLine Journal
Tuberculosis is one of the world's health problems even in Indonesia. The immune response tha... more Tuberculosis is one of the world's health problems even in Indonesia. The immune response that needed to deal with TB can be influenced by other infections, such as helminth infection. The helminth infections induce Th2 immune responses and eosinophilia to eliminate these pathogen. Meanwhile, the Th2 response and eosinophilia can also suppress the Th1 immune response which is very important to eliminate the bacterium M. tuberculosis and make a negative impact on the success of TB therapy. Thus, the eosinophil profile can describe the immune response in TB patients with helminth co-infection. This study aims to determine the prevalence of helminthiasis in TB patients and determine the differences in eosinophil amount in tuberculosis patients with and without helminth co-infection. This study was an observational analytic study with a cross-sectional design conducted at Puskesmas Panti in August 2019-January 2020 using fecal and blood samples from 24 research subjects. Helminth co...
Background: Helminthiasis is a neglected tropical disease and still remain a health problem in tr... more Background: Helminthiasis is a neglected tropical disease and still remain a health problem in tropical and subtropical countries, including Indonesia. According to WHO, approximately 1,5 billion people or 24% of the world's population, are infected with Soil-Transmitted Helminth (STH). Morbidity is directly related to the intensity of STH infestation. Some of the studies shown a significant correlation between eosinophilia and helminthiasis. Eosinophilia on the blood smear examination may be used as a marker of STH infestation. Purpose: to determine the correlation between Soil-Transmitted Helminthiases and eosinophilia as a predictor of morbidity of STH infestation among Widodaren Plantation Workers in Jember. Methods: This observational analytical study use the Kato-Katz method to determine infestation of STH and its intensity measured by egg count per gram stool (EPG). Blood smear examination stained with Giemsa conduct to determine eosinophil count. Results: Prevalence of S...
Dengue Hemorrhagic Fever (DHF) is still one of the major public health problems in the world and ... more Dengue Hemorrhagic Fever (DHF) is still one of the major public health problems in the world and Indonesia. There are several methods of mosquito vector control, one of them is the use of ovitrap and the addition of attractants which is a compound that may attract gravid mosquitoes to lay eggs. Some examples of attractants are hay infusion and sugar fermentation solution. This research aimed to compare the effectiveness of hay infusion and sugar fermentation solution as Aedes aegypti attractants. This research type was a true experimental laboratory with a complete randomized design which is divided into three groups of ovitrap containing aquades as control, 20% hay infusion and 20% sugar fermentation solution. Ovitraps with those attractants were put into a mosquito coop containing 25 gravid female mosquitoes. Mosquito's eggs counting was done after two days with nine times repetition. The data were then analyzed using Kruskal-Wallis and Mann-Whitney test. The difference of mos...
Dental Journal (Majalah Kedokteran Gigi)
Background: Exposure of metals among dental technicians that come from the working environment ca... more Background: Exposure of metals among dental technicians that come from the working environment can lead to the formation reactive oxygen species (ROS). ROS can cause mutations in the p53 gene (p53). The mutation is transversion mutation GuanineThymine. p53 mutations can lead to low expression of the wild-type p53 protein (p53). Wild-type p53 involved in many biological processes such as regulation of genes involved in cell cycle, cell growth after DNA damage, and apoptosis. However, exposure to metals among dental technicians can be prevented through the use of personal protective equipment (PPE) during work. Purpose: The purpose of this study was to analyze the correlation between the use of personal protective equipment to wild-type p53 protein levels among dental technicians in Surabaya. Method: This study was observational analytic with cross sectional approach. 40 samples were taken by random sampling. Data were retrieved through interviews and observations. Wild-type p53 was a...
Jurnal ILMU DASAR, 2014
Malaria infection is initiated when sporozoites are inoculated into a vertebrate host via the sal... more Malaria infection is initiated when sporozoites are inoculated into a vertebrate host via the salivary glands of an Anopheles mosquito. During Anopheles bite, the salivary glands release components that include vasomodulator and immunomodulators. The salivary components of vectors have important role in transmission of pathogen. Therefore, if these components were injected repeatedly into a vertebrate host, it can stimulate host immune system and inhibit the transmission of the pathogen into the host. This could be observed the increasing level of IFN-γ and decreasing level of IL-4 in mice model of malaria after vaccination with salivary gland ekstract (SGE) from An sundaicus s.l. It has also been proven that this mechanisms was related with pathogen of malaria. This was supported by the reduction of parasitemie rate in those mice model after infection by P. Berghei. Keywords: An sundaicus s.l., IFN-γ, IL-4,immunomodulators, salivary gland , TBV
Asian Pacific Journal of Tropical Biomedicine, 2016
Jurnal Medika Planta, May 3, 2013
Introduction. Antimalarial resistance of Plasmodium sp encourage the study of new antimalarial dr... more Introduction. Antimalarial resistance of Plasmodium sp encourage the study of new antimalarial drugs, sush as the traditional medicinal plants used to treat malaria, kembang bulan (Tithonia diversifolia (Hemsley) A. Gray). Kembang bulan ( Tithonia diversifolia (Hemley) A. Gray) ( Asteraceae family) have activity as an antimalarial, anti-inflammatory and analgesic. In vitro, ethanol extract of these leaf had been proven that it was active in inhibiting the growth of P. berghei at doses of 40; 80; 160; and 320 mg/kg body weight with IC 50 values of 114 mg / kg body weight and the IC 90 of 475 mg/kg body weight. Tithonia diversifolia extract contains of substances that could be used as an antimalarial prophylactic and curative in malaria. Objective: to determine the effect of ethanol extract of leaf development as a antimalaria month. Methods is observing the effect of ethanol extract of kembang bulan to parasitemia levels on mice Balb / C before and after infection by the parasite Plasmodium berghei (parasitemia degree of mild, moderate and severe. Result . Based on data from the various percentages of inhibition of extract ethanol of kembang bulan leaf for growth in P. berghei before infected and at various stadium of infection (mild, moderate, and severe), can be seen that in the prophylaxis group had the greatest percentage of inhibition. Conclusion . The Tithonia diversifolia leaves ethanol extract have antimalarial activity in mice Balb/c before inoculated Plasmodium berghei (prophylaxis) and after mild (1%), moderate (5%), and severe (10%).
Planta Medica
Introduction. Antimalarial resistance of Plasmodium sp encourage the study of new antimalarial dr... more Introduction. Antimalarial resistance of Plasmodium sp encourage the study of new antimalarial drugs, sush as the traditional medicinal plants used to treat malaria, kembang bulan (Tithonia diversifolia (Hemsley) A. Gray). Kembang bulan (Tithonia diversifolia (Hemley) A. Gray) (Asteraceae family) have activity as an antimalarial, anti-inflammatory and analgesic. In vitro, ethanol extract of these leaf had been proven that it was active in inhibiting the growth of P. berghei at doses of 40; 80; 160; and 320 mg/kg body weight with IC 50 values of 114 mg / kg body weight and the IC 90 of 475 mg/kg body weight. Tithonia diversifolia extract contains of substances that could be used as an antimalarial prophylactic and curative in malaria. Objective: to determine the effect of ethanol extract of leaf development as a antimalaria month. Methods is observing the effect of ethanol extract of kembang bulan to parasitemia levels on mice Balb / C before and after infection by the parasite Plasmodium berghei (parasitemia degree of mild, moderate and severe. Result. Based on data from the various percentages of inhibition of extract ethanol of kembang bulan leaf for growth in P. berghei before infected and at various stadium of infection (mild, moderate, and severe), can be seen that in the prophylaxis group had the greatest percentage of inhibition. Conclusion. The Tithonia diversifolia leaves ethanol extract have antimalarial activity in mice Balb/c before inoculated Plasmodium berghei (prophylaxis) and after mild (1%), moderate (5%), and severe (10%).
Abstrak Cacingan yang disebabkan oleh soil-transmitted helminths (STH) masih menjadi masalah kese... more Abstrak Cacingan yang disebabkan oleh soil-transmitted helminths (STH) masih menjadi masalah kesehatan yang serius di dunia dan di Indonesia. Indonesia memiliki prevalensi yang bervariasi antara 2,5%-62%. Infeksi ini dapat menyebabkan gangguan pada darah seperti leukositosis, eosinofilia, dan perubahan kadar hemoglobin. Tujuan dari penelitian ini adalah untuk mengetahui gambaran hitung jenis leukosit pada pekerja perkebunan kopi yang terinfeksi STH. Penelitian ini merupakan penelitian deskriptif observasional, dengan pendekatan cross sectional dan dilakukan di perkebunan kopi Kecamatan Silo. Pemeriksaan feses menggunakan metode konsentrasi (sedimentasi dan flotasi) untuk menentukan adanya infeksi STH, sedangkan untuk pemeriksaan hitung jenis leukosit menggunakan metode differential count. Hasil pemeriksaan pada 101 sampel feses menunjukkan 26,7% (27/101) pekerja positif terinfeksi STH, dengan rincian STH jenis hookworm sebesar 92,6% (25/27), dan sisanya infeksi ganda oleh Ascaris lumbricoides dan hookworm sebanyak 7,4% (2/27). Pemeriksaan hitung jenis leukosit menunjukkan, pada infeksi hookworm, 17 pekerja memiliki hitung jenis leukosit yang abnormal yaitu terjadi eosinofilia dan neutrofilia, dan 8 pekerja hasilnya normal. Pada infeksi ganda menunjukkan seluruh pekerja memiliki hasil hitung jenis leukosit yang tidak normal yaitu terjadi eosinofilia dan neutrofilia. Hasil tersebut dapat dipengaruhi oleh kronisitas dari infeksi atau intensitas infeksinya. Kata kunci: STH, perkebunan, hitung jenis leukosit Abstract Helminthiasis caused by soil transmitted helminths (STH) are still a serious health problem in the world and in Indonesia. Indonesia has a prevalence that varies between 2.5%-62%. This infection can cause blood disorders such as leukocytosis, eosinophilia, and changes in hemoglobin levels. The purpose of this study was to describe the leukocyte count of coffee plantation workers that infected by STH. This research was an observational descriptive study, using a cross sectional design and was conducted at the coffee plantation in Silo subdistrict. Stool examination was conducted by the concentration method (sedimentation and flotation) to determine the presence of STH infection, while for leukocyte count, we used the differential count method. The results of examination on 101 feces samples showed 26.7% (27/101) of workers were positively infected with STH, with details of the STH type of hookworm by 92.6% (25/27), and the remaining were double infections by Ascaris lumbricoides and hookworm as much as 7.4 % (2/27). Examination of leukocyte count showed, 17 workers with hookworm infection had abnormal leukocyte count i.e eosinophilia and neutrophilia, while 8 workers were normal. All workers with double infection (2 workers) had abnormal leukocyte count i.e eosinophilia and neutrophilia. These results can be influenced by the chronicity of the infection or the intensity of the infection.
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Papers by Yunita Armiyanti