This study was planned to evaluate the effect of vitamin D administration on cytotoxicity due to ... more This study was planned to evaluate the effect of vitamin D administration on cytotoxicity due to fluoride exposure in vitro. NaF (IC50) and vitamin D (proliferative) were applied to human osteoblast (hFOB 1.19) cells. The major genes of apoptotic, autophagic, and necrotic pathways were determined by RT-PCR. 2-∆∆Ct formulation was used for expression analysis. In the NaF group, caspase 3, Bax, Bad, Bak, Bclx, Atg3, Atg5, Atg6, pG2, LC3-I, LC3-II, RIP1, and RIP3 genes were increased (2.6-15 times). It was observed that the expressions of these genes approached the control when vitamin D was given together with NaF. The Bcl2 gene increased significantly (sixfold) with the effect of NaF, and was down-regulated to some extent with additional vitamin D administration, but still more than in the control. As a result, it was determined that apoptotic, necrotic, and autophagic pathways were activated as the molecular basis of the damage in the bone tissue, which was most affected by fluorine, and these genes were down-regulated and approached the control group with the addition of vitamin D. It was concluded that this is an important data to explain the molecular basis of the protective and therapeutic effect of vitamin D against fluorine toxicity.
Background: Respiratory diseases in calves are causing worldwide economic losses for the beef ind... more Background: Respiratory diseases in calves are causing worldwide economic losses for the beef industry. This study was planned to determine the serum protein fractions and A/G ratio in calves with clinically diagnosed pneumonia, and evaluate the possibility uses of these parameters as clinical diagnostic parameters.Methods: The 34 calves with respiratory system problems and 10 healthy calves without clinical pneumonia symptoms were used as materials. The obtained serum samples were used for total protein and serum protein electrophoresis.Result: Albumin, α1-globulin percentages and A/G ratio decreased in the patient group (p≤0.01), other globulin fractions were higher in the patient group (p≤0.01), for % g values. According to concentration results, it was found that while albumin did not show a significant difference, α1-globulin (p≤0.05) and A / G ratio (p≤0.01) decreased in the patient group. In addition, other globulin fractions and total protein levels were higher in patient gr...
Chronic fluorosis results from long-term fluoride intake at more than the normal doses. The aim o... more Chronic fluorosis results from long-term fluoride intake at more than the normal doses. The aim of this study was to demonstrate the effects of resveratrol (Res) on the serum protein fractions in rats, in which experimental chronic fluorosis was induced. After an adaptation period, the rats were randomly divided into 4 groups of 10; namely the (i) control, (ii) sodium fluoride (NaF), (iii) Res, and (iv) NaF+Res groups. Serum protein fractions in the rat blood samples were determined by cellulose-acetate electrophoresis. While the NaF group had statistically reduced concentrations of total protein, albumin, and α-1 and α-2 globulin compared to the control group (p<0.05), these values were significantly increased (p<0.05) in the NaF+Res group, compared to the NaF group, and close to those of the control group. The βand γ-globulin concentrations were the lowest in the NaF group statistically (p<0.05). Despite a significant increase (p<0.05) in these values in the NaF+Res gr...
ÖZET Amaç: İnflamasyon, flor kaynaklı toksikasyon mekanizmalarının aydınlatılmasında önemli bir a... more ÖZET Amaç: İnflamasyon, flor kaynaklı toksikasyon mekanizmalarının aydınlatılmasında önemli bir adımdır. Bu çalışmada, florozis nefrotoksisitesinde tedavi amacıyla C ve E vitaminlerinin uygulanmasının, inflamasyon gen ekspresyonları üzerine olası etkilerinin ortaya konulması amaçlanmıştır. Materyal ve Metot: Çalışmada 48 adet 200-250 g ağırlığında Wistar-Albino ırkı erkek rat kullanıldı. Kontrol(K), Mısır yağı(M), NaF(NaF), NaF+vitamin E(NVE), NaF+vitamin C(NVC), NaF+vitamin C+vitamin E(NVCE) olmak üzere her biri 8 rat içeren 6 grup oluşturuldu. 16 hafta boyunca 150 mg/kg/gün NaF ratların içme sularına ilave edildi. Vitamin E mısırözü yağında eritilerek verildiği için mısır yağı kontrol grubu oluşturuldu ve ratlara 0,2 ml/gün olarak mısırözü yağı verildi. Tedavi gruplarına 16 hafta NaF verildikten sonra 4 hafta vitamin C (100 mg/kg/gün), vitamin E (300 mg/kg/gün), vitamin C+vitamin E (100 mg/kg/gün+300 mg/kg/gün) oral olarak uygulandı. Deney sonunda tüm gruplarda rat böbrek dokusundan elde edilen RNA izolasyon ürünlerinde, inflamasyon markırlarından IL-1β, TNF-α genlerinin ekspresyonu real time-qPCR ile belirlendi. İnternal kontrol geni olarak beta-aktin (Actb) kullanıldı. Bulgular: TNF-α ekspresyon seviyeleri, en yüksek NaF grubunda bulundu. Diğer tüm gruplarda önemli oranda düşüktü. IL-1β gen ekspresyon düzeyleri; Mısır yağı ve NaF+vitamin C+E gruplarında en düşük, NaF ve NaF+vitamin C gruplarında ise en yüksek bulundu. Sonuç: NaF ile deneysel oluşturulan floroziste artan florürün oluşturduğu olası böbrek hasarında tedavi amaçlı antioksidan vitamin uygulamasının inflamasyonu azalttığı görüldü. Vitamin C, vitamin E, vitamin C+vitamin E'nin inflamasyon markırları üzerinde kayda değer yararlı etkilerinin olduğu tespit edildi. NaF+vitamin C, NaF+vitamin E verilen gruplara kıyasla NaF+vitamin C+E kombinasyonunun inflamasyonu düşürmede daha etkili olduğu görüldü.
Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 2021
Öz: Buzağı ishali, sığır yetiştiriciliğinde ciddi ekonomik kayıplara neden olan önemli sağlık sor... more Öz: Buzağı ishali, sığır yetiştiriciliğinde ciddi ekonomik kayıplara neden olan önemli sağlık sorunlarından biridir. Bu çalışmada, neonatal ishalli buzağılarda serum protein fraksiyonlarının elektroforetik olarak araştırılması planlandı. Bu amaçla, Van ilinde bulunan 7 sığır çiftliğinden temin edilen farklı ırklarda ve cinsiyetlerde, 1-20 günlük ishal belirtileri gösteren 20 buzağı ve kontrol grubu olarak 10 sağlıklı buzağı kullanıldı. V. jugularis'inden alınan kan örneklerinden ayrılan serumlar, selüloz asetat elektroforez yöntemi (Helena Lab-Titan III Serum Protein) ile protein fraksiyonlarına ayrıldı. Elde edilen bantlar Platinum 3.0 programında değerlendirildi. İstatistiksel olarak değerlendirilen sonuçlara göre; serum protein konsantrasyonu bakımından toplam protein, alfa-globülin ve gama-globülin düzeyleri açısından gruplar arasında fark olmadığı (P>0.05) albümin ve beta-globülin düzeylerinin ishal grubunda kontrol grubuna göre daha yüksek olduğu bulundu. Total protein yüzde gram açısından; gruplar arasında albümin, alfa ve beta globülin düzeyleri yönünden anlamlı bir fark görülmedi (P>0.05). Bununla birlikte, albümin/globülin oranının (A/G) ishal grubunda kontrol grubuna göre daha yüksek ve gama globülin düzeylerinin ise daha düşük olduğu tespit edildi. Sonuç olarak, serum protein konsantrasyonlarının ishal grubunda önemli ölçüde değişmediği, ancak A/G oranının arttığı, gama globülinlerin ise azaldığı belirlendi. Anahtar kelimeler: Alfa globülin, asetat elektroforez, gama globülin, serum protein fraksiyonları, yeni doğan ishal Comparison of Serum Protein Fractions in Diarrhea and Healthy Calves Abstract: Calf diarrhea is one of the major health problems causing serious economic losses in cattle breeding. This study was intended to investigate serum protein fractions with electrophoresis methods in neonatal diarrhea. In this study, 20 calves that showed signs of diarrhea for 1-20 days in different breeds and genders obtained from farms in the Van city and 10 healthy calves were used as the control group. Blood samples were taken from the V.jugularis of the animals. The collected serum was separated into protein fractions by cellulose acetate electrophoresis method (Helena Lab-TitanIII Serum Protein). The resulting bands were evaluated in the Platinum 3.0 program. According to the results evaluated statistically; there was no significant difference between the groups in terms of total protein, alpha globulin, and gamma globulin levels (P>0.05). Albumin and beta globulin levels were higher in the diarrhea group than the control group. There was no statistically significant difference (P>0.05) between the groups in albumin, alpha and beta globulin levels in terms of percentage of total protein. However, the albumin/globulin ratio (A/G ratio) was higher in the diarrhea group than that in the control group and gamma globulin level was lower in the diarrhea group. As a result, It was determined that serum protein fractions concentration did not change significantly in the diarrhea group. However, it was determined that the A/G ratio increased, gamma globulins decreased.
Amaç: Diabetes mellitus kan glukoz düzeyinin artmasına bağlı olarak ortaya çıkan metabolik bir ha... more Amaç: Diabetes mellitus kan glukoz düzeyinin artmasına bağlı olarak ortaya çıkan metabolik bir hastalıktır. Bu hastalığa bağlı olarak kan glukoz düzeyinin yanında kan serum parametrelerinde de değişimler gözlenebilmektedir. Bu hastalığın tedavisinde bilinen ilaçların dışında geleneksel bitkisel tedaviler de kullanılmaktadır. Bu çalışma, iki farklı kekik (TVL:Thymus vulgaris L., TSL: Thymbra spicata L.) türünün diyabet oluşturulmuş ratların serum proteinleri üzerindeki etkilerini araştırmak amacıyla gerçekleştirildi. Gereç ve Yöntem: Bu amaçla streptozosin ile deneysel olarak diyabet oluşturulan ratlara farklı dozlarda TVL ve TSL ekstraktları uygulandı. Çalışma; kontrol, diyabet, DMG (diyabetli glibenklamid verilen), TVL1 (diyabet+TVL 100mg/kg), TVL2 (diyabet+TVL 200mg/kg), TSL1 (diyabet+TSL 100mg/kg), TSL2 (diyabet+TSL 200 mg/kg) olmak üzere 7 gruba ayrıldı. Çalışma sonunda elde edilen serum örnekleri, elektroforez yöntemiyle yürütülerek, çalışma gruplarına ait serum proteinlerinin miktar ve oranları tespit edildi. Bulgular: Kontrol grubuna göre konsantrasyon (g/L) bakımından: total protein ve α1-globulin TVL2 ve TSL2 gruplarında önemli oranda azaldığı (p<0.05), albümin düzeyleri; TSL1 ve TVL1 grupları dışında diğerlerinde azalma (p<0.05) gösterdiği, α2-globulin ve β-globulin konsantrasyonlarının; TSL2 grubunda azaldığı (p<0.05), γ-globulinin ise TSL1 grubunda önemli bir artış (p<0.05) gösterdiği belirlendi. Kontrol grubuna göre % olarak: α2-globulinin, deneysel diyabet grubunda önemli oranda arttığı (p<0.05), albuminin, DMG ve TSL1 grubunda azaldığı (p<0.05), α1-globulinin, DMG grubunda önemli oranda arttığı (p<0.05), γ-globulinin TVL2 ve TSL1 gruplarında önemli oranda artış (p<0.05) gösterdiği, albümin/globülin (A/G) oranının ise DMG ve TSL1 gruplarında önemli oranda azaldığı (p<0.05) tespit edildi. Sonuç: Diabetes mellitus tedavisinde kullanılabilecek kekik türleri veya farklı bitki ekstraklarıyla ilgili çalışmalarda serum protein değişimlerinin değerlendirilmesinin önemli olabileceği ve ileriki çalışmalara katkı sağlacağı sonucuna varılmıştır.
Aim: The aim of this study was to determine the effects of lycopene administration as a protectiv... more Aim: The aim of this study was to determine the effects of lycopene administration as a protective agent against necrotic damage of NaF, a fluorine compound found to have high cytotoxic effects in the renal epithelial cell. Material- Method: The renal epithelial cell was replicated in DMEM high glucose medium, containing 10% FBS, 1% L-Glutamine (2mM) and 1% penicillin / streptomycin. With the MTT viability test, the non-toxic dose of lycopene (1 µM) and the IC50 value of NaF at the 24th hour was determined to be 3200 µM. The study groups were divided into four as control, NaF, lycopene and NaF + lycopene (the combination of NaF and lycopene). After the total mRNA obtained from these groups were converted to cDNA, expression levels of the identified necrotic genes were determined by real-time PCR method. Results: While the Ripk 1 gene did not change in the group given lycopene at the 24th hour, it was found that it increased 2.6 times in the group that received only fluorine, while i...
Glioblastoma (GB), en malign ve en agresif beyin tumorudur. Fenotipik ve genotipik acidan yuksek ... more Glioblastoma (GB), en malign ve en agresif beyin tumorudur. Fenotipik ve genotipik acidan yuksek heterojeniteye sahip Glioblastomanin kesin bir tedavi sekli bulunmamaktadir. Glioblastomada kemoterapi uygulamalari tedavide onemli bir yer tutmaktadir. Ancak kemoterapotik direnc tedavi basarisini dusurmektedir. Kemoterapotik direnci azaltmak ve etkili bir kemoterapi ic in bitkisel koken li antikanserojenik mo le kuller elistirilmeye calisilmaktadir. Son yillarda, onemli bir tedavi yaklasimi olara k, bu mo leku llerin tek uygulanmalarinin yaninda kemoterapotik ajanlarla kombine uygulamalari artmaktadir. Bu calis mada, kemoterapotik ajan 5-Fluorourac il ile dogal bir bilesik olan Ce lastrol kombinasyonunun Glioblastomadaki sitotoksik etkisinin arastirilmasi amac lan mistir. MTT sitotoksisite testi sonucunda 2 µM Celastrolun, 50 µM 5-Fluorouracilin sitotoksik etkisini % 19 oraninda arttirdigi, 0,5 ve 2 µM’lik Celastrolun 100 µM’lik 5-Fluorouracilin sitotoksik etkisini sirasiyla % 25 ve %3...
Aim and Background: The present study was planned to investigate the effects of lycopene, on the ... more Aim and Background: The present study was planned to investigate the effects of lycopene, on the caspase-dependent apoptosis in high-dose glucose (HG)-treated PC12 cell line. PC12 cells were cultured in vitro. Materials and Methods: HG was prepared as G (250 mM), and lycopene was prepared as L1 (10 μM), L2 (20 μM), and L3 (40 μM). After 6 h of incubation, the cells were exposed to trypsin, and the samples were obtained with freeze/thaw method. Caspase 3, 8, 9; 8-hydroxy-2-deoxyguanosine (8-OHdG); and M30 were determined (enzyme-linked immunosorbent assay). Results: 8-OHdG increased in L3 (P ≤ 0.001), whereas L1 caused a decrease in HG group (P ≤ 0.001). Caspase-3 decreased significantly in L1, L2, and L3G compared to control (P ≤ 0.001) group. Caspase-8 increased significantly in L1, L1G, L2G, and all L3 glucose groups (P ≤ 0.001). There was no difference for Caspase-9. M30 was not affected by L and HG, which decreased significantly (P ≤ 0.001). Conclusion: As a result, it was deter...
Ionizing radiation (IR) may induce oxidative-stress-related molecular changes (DNA damage, cell d... more Ionizing radiation (IR) may induce oxidative-stress-related molecular changes (DNA damage, cell death, etc.) in living organisms by affecting them directly and indirectly in a time- and dose-dependent manner. This study was planned to present the effects of thymoquinone (TQ) in preventing oxidative stress, DNA damage and apoptotic cell death that may occur after IR exposure. For this purpose, hFOB 1.19 osteoblast cells were used. Using the MTT viability test, the dose of IR reducing the proliferation of cells and the concentration of TQ increasing cell proliferation the most were determined. Five groups were formed as the Control (C), Thymoquinone (TQ), Ionizing radiation (IR), Ionizing radiation + Thymoquinone (IR + TQ) and Thymoquinone + Ionizing radiation (TQ + IR) groups. By applying the determined concentrations on the cells, DNA damage level was determined with the Comet assay, and the gene expression levels of enzymes effective in the oxidative and apoptotic mechanisms were d...
This study was planned to investigate the effects of the antioxidant and protective vitamins A, D... more This study was planned to investigate the effects of the antioxidant and protective vitamins A, D, E, and C, on the expression and translation of certain apoptotic markers in the NRK-52E and hFOB 1.19 cell lines treated with NaF at half the maximal inhibitory concentration (IC50) for 24 hours. The IC50 for NaF and nontoxic vitamin doses were determined by the MTT viability test. For the biochemical assays, cells were harvested by trypsinization and lysed by the freeze/thaw method. The levels and gene expression of caspases 3, 8, and 9, and the levels of M30 and 8OHdG were also measured with methods that included the use of ELISA and qRTPCR. In the MTT studies, compared to the NaF-treated groups, it was found that the cell viability was higher in all the NaF+vitamin D-treated groups in the NRK-52E cell line, in some of the NaF+vitamin D-treated groups in the hFOB 1.19 cell line, and in some of the NaF+vitamin A, E, and C-treated groups for both cell lines. In the NRK52E cell line, th...
The aim of the present study was to investigate the effects of vitamins C (vit C) and E (vit E) o... more The aim of the present study was to investigate the effects of vitamins C (vit C) and E (vit E) on the serum protein fractions in rats with experimentally-induced chronic fluorosis. Wistar-Albino rats (male) were used as material (8 groups, each containing 8 rats). The experimental groups were control, protection groups (daily 150 ppm NaF containing drinking water for 16 weeks as ad-libitum, and, on alternate Vit C (100 mg/kg), Vit E (300 mg/kg) and Vit C+ Vit E (100 mg/kg + 300 mg/kg) for 16 weeks) therapy groups (daily 150 ppm NaF containing drinking water for 16 weeks as ad-libitum, then normal drinking water as ad-libitum, Vit C (100 mg/kg), Vit E (300 mg/kg) and Vit C + Vit E (100 mg/kg + 300 mg/kg) on alternate for 4 weeks). The end of experimental period, blood samples were collected and serums were obtained. Serum protein fractions in blood samples were determined with cellulose-acetate electrophoresis. Alpha-1 was significantly decreased and beta globulins increased in the experimental fluorosis group. In the groups in which vitamin C, vitamin E and combine combination of vitamin C and vitamin E were given for prevention and treatment, it was observed that these changing values approach to the control group.
Objective: The present study was planned to examine the changes in serum protein fractions of lam... more Objective: The present study was planned to examine the changes in serum protein fractions of lambs with pica symptoms with electrophoresis. Materials and Methods: The three study groups were the control group that included healthy lambs, pre-treatment and post-treatment groups that included lamb with pica. Lambs with pica symptoms were then treated with a single capsule mineral complex orally and single dose vitamin complex. Serum protein fractions in blood samples were determined with cellulose-acetate electrophoresis. Results: It was determined that in terms of concentration, total protein, albumin and β-globulin, levels did not change, albumin percentage levels increased with pica, however reached control group levels with treatment and α1-Globulin and α2-Globulin levels reduced after treatment. Based on % gr, it was determined that α1-Globulin and β-Globulin did not change and α2-Globulin decreased. ɣ-Globulin level and percentage increased after treatment. Conclusion: Profile...
Objective: This study was planned to determine the effect of certain vitamin applications on anti... more Objective: This study was planned to determine the effect of certain vitamin applications on antioxidant and oxidant parameters in the osteoblast cell line exposed to sodium fluoride in vitro and to evaluate the protective role of certain vitamins against possible toxic effects of fluoride. Materials and Methods: Cells were replicated in vitro conditions with regular passaging 2-3 times weekly. MTF viability test was used to determine IC 50 of NaF (5000μM) and proliferative doses of vitamins (Vitamin A: 10μM, Vitamin D: 10μM, Vitamin E: 60μM, Vitamin C: 100μM) for hFOB 1.19 cells. Cells were sown in flasks as so to be 10 6 . The study groups were identified as control, NaF, vitamins and NaF+vitamins. After incubation for 24 hours, cells treated with trypsin were prepared by freeze/thaw method and MTT viability test, TAS, SOD, GSH, CAT, TOS and MDA analyzes were performed on these samples. Results: In the hFOB 1.19 cell line, TAS levels decreased significantly in the NaF group (p≤0.0...
Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, 2021
This study was planned to investigate the effects of glutathione, known to be a strong antioxidan... more This study was planned to investigate the effects of glutathione, known to be a strong antioxidant, on caspase-dependent apoptosis and oxidative DNA damage in the kidney cells (BHK-21) exposed to high glucose. BHK-21 cell line was cultivated by regular passages in vitro conditions. Study groups were set up as control, study and its combinations groups ((Glucose; (285 mM, HG), glutathione (250 µM)). After 24 hours of incubation, trypsinized cells were disrupted by freeze-thaw method and analyte was prepared. Caspase 3, 8, 9, M30 and 8-OHdG (oxidative DNA damage marker) levels were determined using commercial ELISA kits. M30, caspase 3, 8 and 9 which are the parameters of apoptosis, were found highest in the HG group. In GSH-treated groups, these parameters decreased slightly. There was no significant difference in the levels of 8-OHdG which is the indicator of oxidative DNA damage.
This study was planned to determine the molecular basis and causes of damage to the kidney and th... more This study was planned to determine the molecular basis and causes of damage to the kidney and the liver, which are the most affected tissues in sheep exposed to chronic fluoride. For this purpose, liver and kidney tissues were obtained from sheep with signs of fluorosis in the age range of 4-6 years. The control group consisted of clinically healthy sheep without fluorosis. The apoptotic and oxidative genes expression of target genes was determined using the real qRT-PCR method. According to the control gene (Gapdh) that was detected that in the liver, the apoptotic genes caspase-8, caspase-9, and Bim increased and caspase-3, Bcl-2, and Bak decreased, while in the kidney, caspase-3 and Bax and caspase-8, Bcl-2, Bcl2l-1, Bim, and Bak decreased. According to the 2-ΔCt values of the oxidative stress genes, it was determined that Cygb, Gstp1, and Ncf1 genes increased significantly in the fluorosis group and Gpx1, sod1, and sod2 genes decreased significantly. In the kidney tissue, Cygb and Gpx1 increased in the fluorosis group, while sod1, sod2, Gstp1, Ncf1 and Ccs, and Nos2 were found to decrease significantly. As a result, it was shown that apoptosis and oxidative mechanisms are activated in the liver and the kidney tissues of sheep with fluorosis and these parameters have an important role in understanding the molecular basis of tissue damage in fluorosis.
This study was planned to evaluate the effect of vitamin D administration on cytotoxicity due to ... more This study was planned to evaluate the effect of vitamin D administration on cytotoxicity due to fluoride exposure in vitro. NaF (IC50) and vitamin D (proliferative) were applied to human osteoblast (hFOB 1.19) cells. The major genes of apoptotic, autophagic, and necrotic pathways were determined by RT-PCR. 2-∆∆Ct formulation was used for expression analysis. In the NaF group, caspase 3, Bax, Bad, Bak, Bclx, Atg3, Atg5, Atg6, pG2, LC3-I, LC3-II, RIP1, and RIP3 genes were increased (2.6-15 times). It was observed that the expressions of these genes approached the control when vitamin D was given together with NaF. The Bcl2 gene increased significantly (sixfold) with the effect of NaF, and was down-regulated to some extent with additional vitamin D administration, but still more than in the control. As a result, it was determined that apoptotic, necrotic, and autophagic pathways were activated as the molecular basis of the damage in the bone tissue, which was most affected by fluorine, and these genes were down-regulated and approached the control group with the addition of vitamin D. It was concluded that this is an important data to explain the molecular basis of the protective and therapeutic effect of vitamin D against fluorine toxicity.
Background: Respiratory diseases in calves are causing worldwide economic losses for the beef ind... more Background: Respiratory diseases in calves are causing worldwide economic losses for the beef industry. This study was planned to determine the serum protein fractions and A/G ratio in calves with clinically diagnosed pneumonia, and evaluate the possibility uses of these parameters as clinical diagnostic parameters.Methods: The 34 calves with respiratory system problems and 10 healthy calves without clinical pneumonia symptoms were used as materials. The obtained serum samples were used for total protein and serum protein electrophoresis.Result: Albumin, α1-globulin percentages and A/G ratio decreased in the patient group (p≤0.01), other globulin fractions were higher in the patient group (p≤0.01), for % g values. According to concentration results, it was found that while albumin did not show a significant difference, α1-globulin (p≤0.05) and A / G ratio (p≤0.01) decreased in the patient group. In addition, other globulin fractions and total protein levels were higher in patient gr...
Chronic fluorosis results from long-term fluoride intake at more than the normal doses. The aim o... more Chronic fluorosis results from long-term fluoride intake at more than the normal doses. The aim of this study was to demonstrate the effects of resveratrol (Res) on the serum protein fractions in rats, in which experimental chronic fluorosis was induced. After an adaptation period, the rats were randomly divided into 4 groups of 10; namely the (i) control, (ii) sodium fluoride (NaF), (iii) Res, and (iv) NaF+Res groups. Serum protein fractions in the rat blood samples were determined by cellulose-acetate electrophoresis. While the NaF group had statistically reduced concentrations of total protein, albumin, and α-1 and α-2 globulin compared to the control group (p<0.05), these values were significantly increased (p<0.05) in the NaF+Res group, compared to the NaF group, and close to those of the control group. The βand γ-globulin concentrations were the lowest in the NaF group statistically (p<0.05). Despite a significant increase (p<0.05) in these values in the NaF+Res gr...
ÖZET Amaç: İnflamasyon, flor kaynaklı toksikasyon mekanizmalarının aydınlatılmasında önemli bir a... more ÖZET Amaç: İnflamasyon, flor kaynaklı toksikasyon mekanizmalarının aydınlatılmasında önemli bir adımdır. Bu çalışmada, florozis nefrotoksisitesinde tedavi amacıyla C ve E vitaminlerinin uygulanmasının, inflamasyon gen ekspresyonları üzerine olası etkilerinin ortaya konulması amaçlanmıştır. Materyal ve Metot: Çalışmada 48 adet 200-250 g ağırlığında Wistar-Albino ırkı erkek rat kullanıldı. Kontrol(K), Mısır yağı(M), NaF(NaF), NaF+vitamin E(NVE), NaF+vitamin C(NVC), NaF+vitamin C+vitamin E(NVCE) olmak üzere her biri 8 rat içeren 6 grup oluşturuldu. 16 hafta boyunca 150 mg/kg/gün NaF ratların içme sularına ilave edildi. Vitamin E mısırözü yağında eritilerek verildiği için mısır yağı kontrol grubu oluşturuldu ve ratlara 0,2 ml/gün olarak mısırözü yağı verildi. Tedavi gruplarına 16 hafta NaF verildikten sonra 4 hafta vitamin C (100 mg/kg/gün), vitamin E (300 mg/kg/gün), vitamin C+vitamin E (100 mg/kg/gün+300 mg/kg/gün) oral olarak uygulandı. Deney sonunda tüm gruplarda rat böbrek dokusundan elde edilen RNA izolasyon ürünlerinde, inflamasyon markırlarından IL-1β, TNF-α genlerinin ekspresyonu real time-qPCR ile belirlendi. İnternal kontrol geni olarak beta-aktin (Actb) kullanıldı. Bulgular: TNF-α ekspresyon seviyeleri, en yüksek NaF grubunda bulundu. Diğer tüm gruplarda önemli oranda düşüktü. IL-1β gen ekspresyon düzeyleri; Mısır yağı ve NaF+vitamin C+E gruplarında en düşük, NaF ve NaF+vitamin C gruplarında ise en yüksek bulundu. Sonuç: NaF ile deneysel oluşturulan floroziste artan florürün oluşturduğu olası böbrek hasarında tedavi amaçlı antioksidan vitamin uygulamasının inflamasyonu azalttığı görüldü. Vitamin C, vitamin E, vitamin C+vitamin E'nin inflamasyon markırları üzerinde kayda değer yararlı etkilerinin olduğu tespit edildi. NaF+vitamin C, NaF+vitamin E verilen gruplara kıyasla NaF+vitamin C+E kombinasyonunun inflamasyonu düşürmede daha etkili olduğu görüldü.
Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 2021
Öz: Buzağı ishali, sığır yetiştiriciliğinde ciddi ekonomik kayıplara neden olan önemli sağlık sor... more Öz: Buzağı ishali, sığır yetiştiriciliğinde ciddi ekonomik kayıplara neden olan önemli sağlık sorunlarından biridir. Bu çalışmada, neonatal ishalli buzağılarda serum protein fraksiyonlarının elektroforetik olarak araştırılması planlandı. Bu amaçla, Van ilinde bulunan 7 sığır çiftliğinden temin edilen farklı ırklarda ve cinsiyetlerde, 1-20 günlük ishal belirtileri gösteren 20 buzağı ve kontrol grubu olarak 10 sağlıklı buzağı kullanıldı. V. jugularis'inden alınan kan örneklerinden ayrılan serumlar, selüloz asetat elektroforez yöntemi (Helena Lab-Titan III Serum Protein) ile protein fraksiyonlarına ayrıldı. Elde edilen bantlar Platinum 3.0 programında değerlendirildi. İstatistiksel olarak değerlendirilen sonuçlara göre; serum protein konsantrasyonu bakımından toplam protein, alfa-globülin ve gama-globülin düzeyleri açısından gruplar arasında fark olmadığı (P>0.05) albümin ve beta-globülin düzeylerinin ishal grubunda kontrol grubuna göre daha yüksek olduğu bulundu. Total protein yüzde gram açısından; gruplar arasında albümin, alfa ve beta globülin düzeyleri yönünden anlamlı bir fark görülmedi (P>0.05). Bununla birlikte, albümin/globülin oranının (A/G) ishal grubunda kontrol grubuna göre daha yüksek ve gama globülin düzeylerinin ise daha düşük olduğu tespit edildi. Sonuç olarak, serum protein konsantrasyonlarının ishal grubunda önemli ölçüde değişmediği, ancak A/G oranının arttığı, gama globülinlerin ise azaldığı belirlendi. Anahtar kelimeler: Alfa globülin, asetat elektroforez, gama globülin, serum protein fraksiyonları, yeni doğan ishal Comparison of Serum Protein Fractions in Diarrhea and Healthy Calves Abstract: Calf diarrhea is one of the major health problems causing serious economic losses in cattle breeding. This study was intended to investigate serum protein fractions with electrophoresis methods in neonatal diarrhea. In this study, 20 calves that showed signs of diarrhea for 1-20 days in different breeds and genders obtained from farms in the Van city and 10 healthy calves were used as the control group. Blood samples were taken from the V.jugularis of the animals. The collected serum was separated into protein fractions by cellulose acetate electrophoresis method (Helena Lab-TitanIII Serum Protein). The resulting bands were evaluated in the Platinum 3.0 program. According to the results evaluated statistically; there was no significant difference between the groups in terms of total protein, alpha globulin, and gamma globulin levels (P>0.05). Albumin and beta globulin levels were higher in the diarrhea group than the control group. There was no statistically significant difference (P>0.05) between the groups in albumin, alpha and beta globulin levels in terms of percentage of total protein. However, the albumin/globulin ratio (A/G ratio) was higher in the diarrhea group than that in the control group and gamma globulin level was lower in the diarrhea group. As a result, It was determined that serum protein fractions concentration did not change significantly in the diarrhea group. However, it was determined that the A/G ratio increased, gamma globulins decreased.
Amaç: Diabetes mellitus kan glukoz düzeyinin artmasına bağlı olarak ortaya çıkan metabolik bir ha... more Amaç: Diabetes mellitus kan glukoz düzeyinin artmasına bağlı olarak ortaya çıkan metabolik bir hastalıktır. Bu hastalığa bağlı olarak kan glukoz düzeyinin yanında kan serum parametrelerinde de değişimler gözlenebilmektedir. Bu hastalığın tedavisinde bilinen ilaçların dışında geleneksel bitkisel tedaviler de kullanılmaktadır. Bu çalışma, iki farklı kekik (TVL:Thymus vulgaris L., TSL: Thymbra spicata L.) türünün diyabet oluşturulmuş ratların serum proteinleri üzerindeki etkilerini araştırmak amacıyla gerçekleştirildi. Gereç ve Yöntem: Bu amaçla streptozosin ile deneysel olarak diyabet oluşturulan ratlara farklı dozlarda TVL ve TSL ekstraktları uygulandı. Çalışma; kontrol, diyabet, DMG (diyabetli glibenklamid verilen), TVL1 (diyabet+TVL 100mg/kg), TVL2 (diyabet+TVL 200mg/kg), TSL1 (diyabet+TSL 100mg/kg), TSL2 (diyabet+TSL 200 mg/kg) olmak üzere 7 gruba ayrıldı. Çalışma sonunda elde edilen serum örnekleri, elektroforez yöntemiyle yürütülerek, çalışma gruplarına ait serum proteinlerinin miktar ve oranları tespit edildi. Bulgular: Kontrol grubuna göre konsantrasyon (g/L) bakımından: total protein ve α1-globulin TVL2 ve TSL2 gruplarında önemli oranda azaldığı (p<0.05), albümin düzeyleri; TSL1 ve TVL1 grupları dışında diğerlerinde azalma (p<0.05) gösterdiği, α2-globulin ve β-globulin konsantrasyonlarının; TSL2 grubunda azaldığı (p<0.05), γ-globulinin ise TSL1 grubunda önemli bir artış (p<0.05) gösterdiği belirlendi. Kontrol grubuna göre % olarak: α2-globulinin, deneysel diyabet grubunda önemli oranda arttığı (p<0.05), albuminin, DMG ve TSL1 grubunda azaldığı (p<0.05), α1-globulinin, DMG grubunda önemli oranda arttığı (p<0.05), γ-globulinin TVL2 ve TSL1 gruplarında önemli oranda artış (p<0.05) gösterdiği, albümin/globülin (A/G) oranının ise DMG ve TSL1 gruplarında önemli oranda azaldığı (p<0.05) tespit edildi. Sonuç: Diabetes mellitus tedavisinde kullanılabilecek kekik türleri veya farklı bitki ekstraklarıyla ilgili çalışmalarda serum protein değişimlerinin değerlendirilmesinin önemli olabileceği ve ileriki çalışmalara katkı sağlacağı sonucuna varılmıştır.
Aim: The aim of this study was to determine the effects of lycopene administration as a protectiv... more Aim: The aim of this study was to determine the effects of lycopene administration as a protective agent against necrotic damage of NaF, a fluorine compound found to have high cytotoxic effects in the renal epithelial cell. Material- Method: The renal epithelial cell was replicated in DMEM high glucose medium, containing 10% FBS, 1% L-Glutamine (2mM) and 1% penicillin / streptomycin. With the MTT viability test, the non-toxic dose of lycopene (1 µM) and the IC50 value of NaF at the 24th hour was determined to be 3200 µM. The study groups were divided into four as control, NaF, lycopene and NaF + lycopene (the combination of NaF and lycopene). After the total mRNA obtained from these groups were converted to cDNA, expression levels of the identified necrotic genes were determined by real-time PCR method. Results: While the Ripk 1 gene did not change in the group given lycopene at the 24th hour, it was found that it increased 2.6 times in the group that received only fluorine, while i...
Glioblastoma (GB), en malign ve en agresif beyin tumorudur. Fenotipik ve genotipik acidan yuksek ... more Glioblastoma (GB), en malign ve en agresif beyin tumorudur. Fenotipik ve genotipik acidan yuksek heterojeniteye sahip Glioblastomanin kesin bir tedavi sekli bulunmamaktadir. Glioblastomada kemoterapi uygulamalari tedavide onemli bir yer tutmaktadir. Ancak kemoterapotik direnc tedavi basarisini dusurmektedir. Kemoterapotik direnci azaltmak ve etkili bir kemoterapi ic in bitkisel koken li antikanserojenik mo le kuller elistirilmeye calisilmaktadir. Son yillarda, onemli bir tedavi yaklasimi olara k, bu mo leku llerin tek uygulanmalarinin yaninda kemoterapotik ajanlarla kombine uygulamalari artmaktadir. Bu calis mada, kemoterapotik ajan 5-Fluorourac il ile dogal bir bilesik olan Ce lastrol kombinasyonunun Glioblastomadaki sitotoksik etkisinin arastirilmasi amac lan mistir. MTT sitotoksisite testi sonucunda 2 µM Celastrolun, 50 µM 5-Fluorouracilin sitotoksik etkisini % 19 oraninda arttirdigi, 0,5 ve 2 µM’lik Celastrolun 100 µM’lik 5-Fluorouracilin sitotoksik etkisini sirasiyla % 25 ve %3...
Aim and Background: The present study was planned to investigate the effects of lycopene, on the ... more Aim and Background: The present study was planned to investigate the effects of lycopene, on the caspase-dependent apoptosis in high-dose glucose (HG)-treated PC12 cell line. PC12 cells were cultured in vitro. Materials and Methods: HG was prepared as G (250 mM), and lycopene was prepared as L1 (10 μM), L2 (20 μM), and L3 (40 μM). After 6 h of incubation, the cells were exposed to trypsin, and the samples were obtained with freeze/thaw method. Caspase 3, 8, 9; 8-hydroxy-2-deoxyguanosine (8-OHdG); and M30 were determined (enzyme-linked immunosorbent assay). Results: 8-OHdG increased in L3 (P ≤ 0.001), whereas L1 caused a decrease in HG group (P ≤ 0.001). Caspase-3 decreased significantly in L1, L2, and L3G compared to control (P ≤ 0.001) group. Caspase-8 increased significantly in L1, L1G, L2G, and all L3 glucose groups (P ≤ 0.001). There was no difference for Caspase-9. M30 was not affected by L and HG, which decreased significantly (P ≤ 0.001). Conclusion: As a result, it was deter...
Ionizing radiation (IR) may induce oxidative-stress-related molecular changes (DNA damage, cell d... more Ionizing radiation (IR) may induce oxidative-stress-related molecular changes (DNA damage, cell death, etc.) in living organisms by affecting them directly and indirectly in a time- and dose-dependent manner. This study was planned to present the effects of thymoquinone (TQ) in preventing oxidative stress, DNA damage and apoptotic cell death that may occur after IR exposure. For this purpose, hFOB 1.19 osteoblast cells were used. Using the MTT viability test, the dose of IR reducing the proliferation of cells and the concentration of TQ increasing cell proliferation the most were determined. Five groups were formed as the Control (C), Thymoquinone (TQ), Ionizing radiation (IR), Ionizing radiation + Thymoquinone (IR + TQ) and Thymoquinone + Ionizing radiation (TQ + IR) groups. By applying the determined concentrations on the cells, DNA damage level was determined with the Comet assay, and the gene expression levels of enzymes effective in the oxidative and apoptotic mechanisms were d...
This study was planned to investigate the effects of the antioxidant and protective vitamins A, D... more This study was planned to investigate the effects of the antioxidant and protective vitamins A, D, E, and C, on the expression and translation of certain apoptotic markers in the NRK-52E and hFOB 1.19 cell lines treated with NaF at half the maximal inhibitory concentration (IC50) for 24 hours. The IC50 for NaF and nontoxic vitamin doses were determined by the MTT viability test. For the biochemical assays, cells were harvested by trypsinization and lysed by the freeze/thaw method. The levels and gene expression of caspases 3, 8, and 9, and the levels of M30 and 8OHdG were also measured with methods that included the use of ELISA and qRTPCR. In the MTT studies, compared to the NaF-treated groups, it was found that the cell viability was higher in all the NaF+vitamin D-treated groups in the NRK-52E cell line, in some of the NaF+vitamin D-treated groups in the hFOB 1.19 cell line, and in some of the NaF+vitamin A, E, and C-treated groups for both cell lines. In the NRK52E cell line, th...
The aim of the present study was to investigate the effects of vitamins C (vit C) and E (vit E) o... more The aim of the present study was to investigate the effects of vitamins C (vit C) and E (vit E) on the serum protein fractions in rats with experimentally-induced chronic fluorosis. Wistar-Albino rats (male) were used as material (8 groups, each containing 8 rats). The experimental groups were control, protection groups (daily 150 ppm NaF containing drinking water for 16 weeks as ad-libitum, and, on alternate Vit C (100 mg/kg), Vit E (300 mg/kg) and Vit C+ Vit E (100 mg/kg + 300 mg/kg) for 16 weeks) therapy groups (daily 150 ppm NaF containing drinking water for 16 weeks as ad-libitum, then normal drinking water as ad-libitum, Vit C (100 mg/kg), Vit E (300 mg/kg) and Vit C + Vit E (100 mg/kg + 300 mg/kg) on alternate for 4 weeks). The end of experimental period, blood samples were collected and serums were obtained. Serum protein fractions in blood samples were determined with cellulose-acetate electrophoresis. Alpha-1 was significantly decreased and beta globulins increased in the experimental fluorosis group. In the groups in which vitamin C, vitamin E and combine combination of vitamin C and vitamin E were given for prevention and treatment, it was observed that these changing values approach to the control group.
Objective: The present study was planned to examine the changes in serum protein fractions of lam... more Objective: The present study was planned to examine the changes in serum protein fractions of lambs with pica symptoms with electrophoresis. Materials and Methods: The three study groups were the control group that included healthy lambs, pre-treatment and post-treatment groups that included lamb with pica. Lambs with pica symptoms were then treated with a single capsule mineral complex orally and single dose vitamin complex. Serum protein fractions in blood samples were determined with cellulose-acetate electrophoresis. Results: It was determined that in terms of concentration, total protein, albumin and β-globulin, levels did not change, albumin percentage levels increased with pica, however reached control group levels with treatment and α1-Globulin and α2-Globulin levels reduced after treatment. Based on % gr, it was determined that α1-Globulin and β-Globulin did not change and α2-Globulin decreased. ɣ-Globulin level and percentage increased after treatment. Conclusion: Profile...
Objective: This study was planned to determine the effect of certain vitamin applications on anti... more Objective: This study was planned to determine the effect of certain vitamin applications on antioxidant and oxidant parameters in the osteoblast cell line exposed to sodium fluoride in vitro and to evaluate the protective role of certain vitamins against possible toxic effects of fluoride. Materials and Methods: Cells were replicated in vitro conditions with regular passaging 2-3 times weekly. MTF viability test was used to determine IC 50 of NaF (5000μM) and proliferative doses of vitamins (Vitamin A: 10μM, Vitamin D: 10μM, Vitamin E: 60μM, Vitamin C: 100μM) for hFOB 1.19 cells. Cells were sown in flasks as so to be 10 6 . The study groups were identified as control, NaF, vitamins and NaF+vitamins. After incubation for 24 hours, cells treated with trypsin were prepared by freeze/thaw method and MTT viability test, TAS, SOD, GSH, CAT, TOS and MDA analyzes were performed on these samples. Results: In the hFOB 1.19 cell line, TAS levels decreased significantly in the NaF group (p≤0.0...
Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, 2021
This study was planned to investigate the effects of glutathione, known to be a strong antioxidan... more This study was planned to investigate the effects of glutathione, known to be a strong antioxidant, on caspase-dependent apoptosis and oxidative DNA damage in the kidney cells (BHK-21) exposed to high glucose. BHK-21 cell line was cultivated by regular passages in vitro conditions. Study groups were set up as control, study and its combinations groups ((Glucose; (285 mM, HG), glutathione (250 µM)). After 24 hours of incubation, trypsinized cells were disrupted by freeze-thaw method and analyte was prepared. Caspase 3, 8, 9, M30 and 8-OHdG (oxidative DNA damage marker) levels were determined using commercial ELISA kits. M30, caspase 3, 8 and 9 which are the parameters of apoptosis, were found highest in the HG group. In GSH-treated groups, these parameters decreased slightly. There was no significant difference in the levels of 8-OHdG which is the indicator of oxidative DNA damage.
This study was planned to determine the molecular basis and causes of damage to the kidney and th... more This study was planned to determine the molecular basis and causes of damage to the kidney and the liver, which are the most affected tissues in sheep exposed to chronic fluoride. For this purpose, liver and kidney tissues were obtained from sheep with signs of fluorosis in the age range of 4-6 years. The control group consisted of clinically healthy sheep without fluorosis. The apoptotic and oxidative genes expression of target genes was determined using the real qRT-PCR method. According to the control gene (Gapdh) that was detected that in the liver, the apoptotic genes caspase-8, caspase-9, and Bim increased and caspase-3, Bcl-2, and Bak decreased, while in the kidney, caspase-3 and Bax and caspase-8, Bcl-2, Bcl2l-1, Bim, and Bak decreased. According to the 2-ΔCt values of the oxidative stress genes, it was determined that Cygb, Gstp1, and Ncf1 genes increased significantly in the fluorosis group and Gpx1, sod1, and sod2 genes decreased significantly. In the kidney tissue, Cygb and Gpx1 increased in the fluorosis group, while sod1, sod2, Gstp1, Ncf1 and Ccs, and Nos2 were found to decrease significantly. As a result, it was shown that apoptosis and oxidative mechanisms are activated in the liver and the kidney tissues of sheep with fluorosis and these parameters have an important role in understanding the molecular basis of tissue damage in fluorosis.
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