Papers by Vladimir Miklic
Interdependence between yield and yield components of the confectionary sunflower
Towards Orobanche resistance in sunflower-SSR analysis of a novel resistance source Ivana Imerovski
Influence of pollination method, pollen viability and nectar content on sunflower seed yield
Selekcija i semenarstvo, 2014
Izvod Rezultati prinosa semena i ulja dobijenih iz ogleda i sa proizvodnih parcela izdvojili su h... more Izvod Rezultati prinosa semena i ulja dobijenih iz ogleda i sa proizvodnih parcela izdvojili su hibride suncokreta NS Oskar i NS Fantazija kao vrlo perspektivne i potencijalno vodeće u Srbiji. Hibridi su stvoreni procesom selekcije u Institutu za ratarstvo i povrtarstvo, Novi Sad. Hibridi priradaju grupi srednje kasnih tj. srednje ranih hibrida suncokreta, pokazuju visok potencijal rodnostii imaju visok sadržaj ulja u semenu. Prilagođeni su gajenju u različitim agroekološkim uslovima jer imaju široku adaptabilnost na različite uslove proizvodnje. Imaju i visok stepen otpornosti i tolerantnosti prema najznačajnijim parazitima suncokreta u regionu, kao što su Phomopsis, bela trulež, rđa, volovod i dr. Seme hibrida je odličnog kvaliteta i klijavosti. U radu su prikazani rezultati prinosa semena komisijskih ogleda Odeljenja za priznavanje sorti Ministarstva poljoprivrede i zaštite životne sredine Republike Srbije, kao i rezultati ostvareni u mikro ogledima Instituta za ratarstvo i povrtarstvo i poljoprivrednim gazdinstvima u Srbiji.
Broomrape (Orobanche cumana Wallr.) is considered to be one of the most severe constrains in su... more Broomrape (Orobanche cumana Wallr.) is considered to be one of the most severe constrains in sunflower (Helianthus annuus L.) production. Rapid appearance of new races requires continuous search for new sources of resistance in sunflower germplasm. The objective of this research was to screen new sunflower inbred lines, originating from different interspecific hybridisations or Russian open pollinated varieties, for broomrape resistance using different races of parasite. Newly developed sunflower cms and Rf lines were screened for resistance to broomrape in greenhouse and in field trials in two locations in Serbia, infested with the race E of the parasite, during the period 2007-2010. Research was broadened in 2011 to locations in Spain and Romania which are previously known for presence of highly virulent races F and G. Differential lines, up to race F, were planted together with selected inbred lines. Reaction of the tested sunflower lines to broomrape was evaluated by calculating incidence, i.e. ratio of infected sunflower plants per total number of plants. Tested inbred lines significantly differed regarding their resistance to broomrape. In the first period of research, resistant inbred lines were selected. The experiment performed in five locations in three countries (Serbia, Spain and Romania) showed different reaction of selected sunflower inbred lines to broomrape, which depended on location i.e. race composition of the broomrape. Selected inbred lines were completely resistant to broomrape race E, present in Northern Serbia. Only several inbred lines showed resistance to races present in location in Spain and Romania. Also, differential lines were mostly susceptible to parasite indicating presence of race which overcome race F. Based on broomrape incidence of tested inbred lines and differentials, race G is present in locations in Spain and Romania and maybe more aggressive race.
Journal of Agricultural Sciences, Belgrade, 2015
Rezime: Cilj rada bio je da se ispita uticaj biostimulatora, sa ili bez upotrebe fungicida, na pa... more Rezime: Cilj rada bio je da se ispita uticaj biostimulatora, sa ili bez upotrebe fungicida, na parametre kvaliteta semena i rani porast klijanca suncokreta. Testiranje je sprovedeno u Institutu za ratarstvo i povrtarstvo u Novom Sadu na citoplazmatski muško sterilnoj liniji suncokreta HA-26-PR. U ogled je bilo uključeno šest tretmana: Slavol S (25%), Slavol S (25%) + fungicid (a.m. metalaksil-m), Bioplant Flora (2%), Bioplant Flora (2%) + fungicid (a.m. metalaksil-m), Slavol S (25%) + Bioplant Flora (2%), Slavol S (25%) + Bioplant Flora (2%) + fungicid (a. m. metalaksil-m) i kontrola. Rezultati istraživanja pokazali su da tretiranje semena biostimulatorima pre setve ima značajan uticaj na parametre klijanja semena i rani porast klijanca suncokreta. Pojedinačna primena preparata, sa ili bez upotrebe fungicida, dala je pozitivan efekat, dok je njihova kombinacija dovela do negativnog dejstva. Pojedinačnom primenom preparata, sa ili bez kombinacije sa fungicidom, može se povećati energija klijanja za 5%, klijavost za 6%, ali i skratiti vreme klijanja semena. Takođe, njihovom upotrebom ostvaruje se pozitivan efekat i na rani porast klijanca suncokreta. Ključne reči: energija klijanja, klijavost semena, dužina klijanca, masa klijanca. Uvod Prva istraživanja vezana za primenu biostimulatora u biljnoj proizvodnji odnosila su se na ekstrakt morske alge (Ascophyllum nodosum), huminske kiseline, tiamina i askorbata, na više vrsta povrtarskih kultura uzgajanih u kontrolisanim uslovima (Zeljković, 2013). Biostimulatori su supstance koje
Ratarstvo i povrtarstvo, 2011
Izvod: Ogled je postavljen 2009. na Rimskim Šančevima sa ciljem da se ispita uticaj hemijske desi... more Izvod: Ogled je postavljen 2009. na Rimskim Šančevima sa ciljem da se ispita uticaj hemijske desikacije na masu 1000 semena kod tri linije suncokreta, kao i da se utvrdi optimalni momenat za njeno izvođenje. Desikacija je vršena preparatom Reglone forte sa dozom 2 l/ha. Tretiranje je vršeno svakih 7 dana, počev od završetka cvetanja do postizanja žetvene zrelosti. Kod sve tri linije utvrđena je visoko značajna razlika u masi 1000 semena, kao i visoko značajan uticaj momenta desikacije na masu 1000 semena. Najniža masa utvrđena je kod tretmana 7 dana posle cvetanja, a najviša kod tretmana 28 dana posle cvetanja, pri vlažnosti semena od 22,23%. Može se zaključiti da je sa aspekta mase 1000 semena, upravo to optimalno vreme za izvođenje hemijske desikacije. Između tretiranja 28 dana posle cvetanja i kasnijih tretmana nije bilo značajne razlike u masi 1000 semena, što govori da je završeno nakupljanje rezervnih materija u semenu.
Helia, 2012
Sunflower is a plant species with a prominent period of seed dormancy. Physiological maturation p... more Sunflower is a plant species with a prominent period of seed dormancy. Physiological maturation period can be accelerated under laboratory conditions using methods for breaking seed dormancy such as: previous cooling at 5-10°C, previous heating at 30-35°C, gibberellic acid treatment, KNO 3 , ethrel, etc. The aim of this study was to determine the duration of seed dormancy period of different sunflower hybrids and parent lines produced in Serbia, and to determine which of the methods for breaking seed dormancy gives the best results. Studies were carried out on five commercial sunflower hybrids and their parental components. Seed germination was determined after harvesting using standard laboratory methods and tetrazolium tests. The following methods were used for seed dormancy breaking: low temperature treatment of 5-10°C, high temperature treatment of 30-35°C, and treatment with gibberellic acid. The tests were performed in the period of 30, 50, and 90 days after harvesting. The application of gibberellic acid method gave the lowest values of seed germination, while statistical significant differences between the control and method of low and high temperatures were not found. Sunflower hybrids Sremac, Duško, NS-H-111, Velja and Branko, as well as the female lines of hybrid NS-H-111, and male lines of hybrids Sremac and Velja had no prominent seed dormancy 30 days after harvesting. Seed dormancy in female lines of hybrids Sremac, Duško, Velja and Branko and male lines of hybrids Duško, NS-H-111 and Branko was decreased 90 days after storage, but it was not completely removed.
Helia, 2012
Broomrape (Orobanche cumana Wallr.) presents a serious problem in sunflower production in a numbe... more Broomrape (Orobanche cumana Wallr.) presents a serious problem in sunflower production in a number of countries, it reduces sunflower seed yield and negatively affects other sunflower traits in sunflower production. Continued work on creating new sunflower hybrids resistant to broomrape requires testing of breeding materials in both field conditions and controlled conditions of a greenhouse. The best solution is to do the testing simultaneously in order to verify the congruence of the evaluation of the broomrape resistance achieved by these two methods. The screening of chosen new 15 sunflower hybrids for broomrape resistance was done in 2009 and 2010 in infected field on two locations in Vojvodina Province and in 2010 in greenhouse. Both field and the greenhouse tests rated the hybrids exclusively on the basis of presence or absence of the parasite. The infection of hybrids with broomrape has shown that out of 15 hybrids tested, 9 hybrids were resistant. These hybrids were fully resistant in both years in field conditions and controlled greenhouse conditions during one year. Fully resistant hybrids in field conditions and in greenhouse conditions NS-H-6385 and NS-H-6396 had significantly higher seed yield, seed oil content and oil yield in relation to standard.
Helia, 2011
Four interspecies populations, originating from three annual (H. debilis, H. praecox, H. desertic... more Four interspecies populations, originating from three annual (H. debilis, H. praecox, H. deserticola) and one perennial (H. resinosus) wild species, were used to produce 13 new (cms) inbred lines with cytoplasmic male sterility, three Rf restorer lines with good general combining ability used as testers and their F 1 hybrids. A two-year trial with three replicates was set up using the line × tester method. The inbred A-lines, Rf-testers and F 1 hybrids differed significantly in the mean values for both studied traits. The mode of inheritance for leaf area was dominance and superdominance of the better parent and for plant height it was superdominance of the better parent. Highly significant positive values of general combining ability for both traits were found in inbred lines NS-G-7, NS-G-8, NS-G-9. The greatest highly significant positive specific combining ability value was found in NS-G-6 × RUS-Rf-OL-168 for the total leaf area per plant and in NS-G-6 × RHA-N-49 for plant height. The non-additive gene effect had greater influence on both traits. A significant heterotic effect was found for both traits in relation to the parental average so as in relation to the better parent.
Helia, 2011
For much of the past century, mutagenesis has gained popularity in plant genetics research as a m... more For much of the past century, mutagenesis has gained popularity in plant genetics research as a means of inducing novel genetic variation. Induced mutations have been applied for the past 40 years to produce mutant cultivars in sunflower by changing plant characteristics that significantly increase plant yield and quality. The present study is focused on generating baseline data to elucidate the role of genotypic differences in the response of sunflower to induced mutagenesis with the aim of expanding the applicability of the use of induced mutant stocks in the genetic improvement of the crop and in its functional genomics. The strategy adopted was to estimate the optimal treatment conditions (doses of mutagens) through relating the extent of damage in seedling progeny to the exposure levels of the initiating propagates to mutagens. Seeds of fifteen elite sunflower genotypes of commonly used as breeding stocks and grown on commercial scales were treated with a range of mutagens: gamma rays (γ rays); fast neutrons and with ethyl-methane-sulphonate (EMS) at different treatment doses. The three mutagenic agents affected seedling height, reducing it with increasing dosage. Based on the mutagen damage on seedling height, the 50% and 30% damage indices (D 50 and D 30 , respectively) were estimated for the 15 sunflower genotypes for the three mutagens. The D 50 (D 30) values for the sunflower lines ranged from 120 to 325 Gy (5 to 207 Gy) for gamma irradiation; 9 to 21 Gy (0.1 to 10 Gy) for fast neutrons and 0.69 to 1.55% (0.01 to 0.68%) concentration of EMS.
Helia, 2010
Hybrid sunflower seed, regularly protected with chemicals, is used for sowing in the second or th... more Hybrid sunflower seed, regularly protected with chemicals, is used for sowing in the second or the third year if not used in the first year after production. In that case, it is stored in warehouses, and the length of storage period may significantly affect the quality of seeds. An experiment was conducted in Laboratory for Seed Testing of Institute of Field and Vegetable Crops in Novi Sad, in order to examine the effect of storage period on germination of treated sunflower seeds of three commercial hybrids of the Novi Sad Institute. The seed was treated with fungicides-metalaxyl and fludioxonil, as well as with insecticides-thiamethoxam and imidacloprid, while untreated seeds served as control. The sowing was repeated throughout the year, at three-month intervals. The obtained results indicated that, on average, the hybrid Sremac had the highest (94.61%), and the hybrid Šumadinac the lowest seed germination (90.29%). After one year of storage, seed germination declined significantly. All three hybrids treated with fungicides and the control had a significantly higher germination than hybrids treated with insecticides. This regularity was not evident right after the treatment-it became noticeable during the storage.
Helia, 2010
In the present study, thirteen SSRs (Simple Sequence Repeats) were employed for screening the lev... more In the present study, thirteen SSRs (Simple Sequence Repeats) were employed for screening the level of similarity between sunflower parental lines and progeny obtained in final stages of selection. Another aim of the study was to compare the success of making isogenic lines through selection. Line P (donor line) was used as a donor of downy mildew gene Pl 6 and backcrossed with four commercial inbred lines (A, B, C and D) susceptible to downy mildew. HAP3, a DNA-specific primer, was used to test the success of transfer of Pl 6 gene from the donor line to the progeny lines in the F 6 and F 7 generations. It was found that all progeny lines were resistant to downy mildew. Results obtained by using thirteen primers showed that progeny lines were similar to their respective recipient parent, but further studies have to be conducted using a larger number of primers and a larger number of genotypes.
Helia, 2010
Sunflower is attacked by more than 40 different diseases of which only a certain number causes se... more Sunflower is attacked by more than 40 different diseases of which only a certain number causes serious reduction of seed yield. One of the most damaging diseases is downy mildew, which is caused by the fungus Plasmopara halstedii (Farl.) Berl. et de Toni (syn. Plasmopara helianthi Novot.). Sunflower downy mildew has great economic importance in all countries where sunflower is grown. When the meteorological conditions during the vegetation period of sunflower become favourable for disease development, the damages produce considerable reducing of the seed yield and the oil content. The best way of controlling the fungus is to grow resistant cultivars and because of that the major objective of this study was to develop sunflower genotypes genetically resistant to dominant races of downy mildew in Serbia. During this work two co-dominant CAPS markers for Pl 6 gene were developed which can also be used for Pl 7 gene. For introduction of these genes in breeding program marker assisted selection (MAS) was used. Developed commercial sunflower inbred lines exhibit resistance to all known races of downy mildew in Serbia indicated incorporation of resistance to downy mildew in well-known and widely produced hybrids. Besides that, Pl-genes were introduced to a large number of new inbred lines and new downy mildew resistant hybrids. These new hybrids reach higher seed and oil yields then hybrids widely produced.
Helia, 2010
Broomrape (Orobanche cumana Wallr.) presents a serious problem in sunflower production in a numbe... more Broomrape (Orobanche cumana Wallr.) presents a serious problem in sunflower production in a number of countries. The population of broomrape has been stable in Serbia for a long period of time, but the racial composition has changed in recent years, with race E being predominant in the regions of north Bačka and Banat. Cultivated sunflower is genetically narrow and deficient in many desirable genes. Sources of resistance genes to broomrape can be found in a number of wild sunflower species. New 7 Rf inbred lines (RHA-D
Helia, 2010
Path coefficient analysis was used to separate direct and indirect effects of studied traits on s... more Path coefficient analysis was used to separate direct and indirect effects of studied traits on seed oil content and seed yield, and to identify traits that could be used as selection criteria in sunflower breeding. Significant and highly significant correlations were found between most of the examined traits. Highly significant negative correlations have been established between stem diameter, total leaf area per plant, head diameter and 1,000-seed weight on one side and seed oil content on the other. Highly significant positive correlations have been established between seed yield on one side and stem diameter, total leaf area per plant, head diameter, total number of seeds per head, and the 1,000-seed weight on the other. The weight of 1,000 seeds had a highly significant direct negative effect on seed oil content and a highly significant direct positive effect on seed yield. The total number of seeds per head has demonstrated a highly significant direct positive effect on seed yield and oil content. The total leaf number per plant has demonstrated a significant direct positive effect on seed yield. A path coefficient analysis of seed yield at phenotypic level indicated that maximum direct effects were shownby the weight of 1,000 seeds, total number of seeds per head and total leaf number per plant. The total number of seeds per head was the most important trait that determined seed yield and oil content. The obtained results can be used in sunflower breeding programs aimed at the development of sunflower hybrids that combine high genetic potentials for seed yield and seed oil content.
Helia, 2006
Effect of harvest date on seed viability has been examined in 3 different sunflower genotypes. Ha... more Effect of harvest date on seed viability has been examined in 3 different sunflower genotypes. Harvesting started 7 days after fertilization. It was done 10 times, at 3-4-day intervals. Seed moisture was determined before each harvest. Trials were conducted in India and Serbia. Seed viability was determined 3 months after the harvest. Genotype ranking regarding seed viability, was not the same in the two locations; higher average seed germination was registered in Serbia. There were no significant increases in seed viability once the average seed moisture at the time of harvest reached 41% (India) and 48% (Serbia). Differences existed between the locations in minimum and maximum daily temperatures. Regression analysis showed that highest seed germination rates were reached when seed moisture content at harvest went below 32%, in most cases when seed moisture reached 22-23%.
Helia, 2009
One of the directions in sunflower breeding is the selection for resistance to broomrape. The pop... more One of the directions in sunflower breeding is the selection for resistance to broomrape. The population of broomrape has been stable in Serbia for a long period of time, but the racial composition has changed in recent years, with race E being predominant in the regions of north Bačka and Banat. Cultivated sunflower is genetically narrow and deficient in many desirable genes. Its genetic variability can be increased by the use of wild sunflower species and interspecific hybridization. The resistance to broomrape in 15 new experimental hybrids was tested on locations in Serbia (Pačir) during three years (2006, 2007 and 2008) and in Romania (Baragan, Braila) during 2008. These hybrids were produced by crossing female inbred lines (Ha-26PR-A, PH-BC 2-92-A and Ha-98-A) susceptible to broomrape race E and new Rf inbred lines (RHA-D-2, RHA-D-5, RHA-D-6, RHA-D-7, RHA-D-8), developed from interspecific populations originating from H. deserticola and resistant to race E. All examined hybrids were resistant to broomrape in both locations. Since broomrape race F is present in the location in Romania, it is expected that the new Rf inbred lines-male components of the examined hybrids, possess the resistance gene for race F also.
Ratarstvo i povrtarstvo, 2014
The aim of this paper was to examine the effect of biostmulators on seed quality parameters-germi... more The aim of this paper was to examine the effect of biostmulators on seed quality parameters-germination energy and germination, depending on the genotype and age of sunflower seeds. Testing has been conducted at the Institute of Field and Vegetable Crops on four cytoplasmic male sterile lines: OCMS-98 (L1), HA-NS-26 (L2), PH-BC2-74 (L3) and VL-A-8 (L4). Seed was produced in the period from 2010 to 2012. Two seed variants were tested-treated with metalaxyl-m and non-treated. Prior to sowing, seed was treated with fertilizes Slavol S, Bioplant Flora, and their combination. Selecting the right biostimulator for a particular genotype may lead to increased germination energy and germination, which has the positive effect on the number of plants per unit area, and hence the yield.
Sunflower genotypes tolerance to charcoal rot (Macrophomina phaseolina (Tassi) Goid.) under the field conditions
Genetika
Sunflower (Helianthus annuus L.) is one of the most important crops grown in the world, but it ca... more Sunflower (Helianthus annuus L.) is one of the most important crops grown in the world, but it can be attacked by more than 30 different pathogens. The areal of Macrophomina phaseolina (Tassi) Goid., one of the sunflower pathogens nowadays often found in temperate regions, has been spreading over the last few years due to climate change. The most effective eco-friendly method for controlling charcoal rot caused by M. phaseolina is growing resistant sunflower cultivars. Due to that, 24 commercially available hybrids and 70 inbred lines were tested for M. phaseolina tolerance in a two-year trial. Under the field conditions, two different inoculation methods were used - the Unwounded Stem Base Inoculation (USBI) and Toothpick (TP) method. This study identified five highly tolerant hybrids and 12 inbred lines that can be used in breeding programs for improvement of future genotypes. Sunflower genotype screening tests for Macrophomina tolerance indicated that both inoculation methods sho...
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Papers by Vladimir Miklic