Papers by Vladimir Biocanin
Stomatoloski Glasnik Srbije, 2023
Gram-negative bacteria include more than 20 genera. The most commonly isolated genera are: Bacter... more Gram-negative bacteria include more than 20 genera. The most commonly isolated genera are: Bacteriodes spp., Porphiromonas spp., Fusobacterium spp. and Prevotella spp. The following genera were isolated somewhat less frequently: Tanerella spp., Leptotricha spp., Veilonella spp.,Wollinela spp., Selenomonas spp. and Treponema spp. Anaerobic bacteria have an anaerobic type of metabolism and therefore their incubation is significantly longer and more demanding than aerobic bacteria. The genera Prevotella spp., Porphyromonas spp. and Fusobacterium spp. are part of the resident flora of the oral cavity and in unfavorable conditions cause periodontal diseases, and sometimes dentogenic infections and systemic diseases, such as Alzheimer's disease, cardiovascular diseases, metabolic diseases and inflammatory bowel diseases. The three most important steps for the successful diagnosis of anaerobic bacteria are: proper sampling with avoiding sample contamination, rapid transport of samples to the microbiological laboratory and proper handling of samples. A combination of beta-lactam with the addition of beta-lactamase inhibitors, metronidazole, clindamycin and moxifloxacin is used for treatment for infections caused by anaerobic Gram-negative bacteria. It is important to note that antibiotics should be used only with a clear indication and to choose the right antibiotic in the optimal dose. The aim of this review is to point out the role of Gram-negative anaerobic bacteria in periodontal diseases, and its isolation, identification and antibiotic susceptibility.
Journal of Infection in Developing Countries, May 31, 2023
Introduction: This study aimed to investigate the possibility of applying the hyperlight fluid fu... more Introduction: This study aimed to investigate the possibility of applying the hyperlight fluid fusion essential complex in dental plaque control, and to evaluate the effectiveness of new and modern agents used for the prevention and early treatment of gingivitis. Methodology: The study included 60 subjects randomly divided into two groups. The control group was assigned to 0.12% chlorhexidine (CHX) mouth rinse, whereas the test group used a solution based on hyper-harmonized hydroxylated fullerene water complex (3HFWC), twice daily for 2 weeks. The plaque, gingivitis and bleeding scores were evaluated and recorded. Collected plaque samples were seeded on blood agar and incubated aerobically at a temperature of 37 °C for 24-48 hours. In order to isolate anaerobic bacteria, samples were seeded on Schaedler Agar and incubated anaerobically at 37 °C for seven days. Serial dilutions in saline from 10 1-10 6 were made, and grown colonies were counted and identified using the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF) system. Results: The reduction in the number of bacteria was significant in both control and test groups. The reduction was greater in the control group compared to the experimental group, but without statistically significant difference. Conclusions: 3HFWC treatment causes significant reduction in the number of dental plaque microorganisms. Since 3HFWC solution exhibited a bacteriostatic effect similar to chlorhexidine it could be an adequate addition to solution of a growing problem in prevention and early treatment of gingivitis and periodontitis.
Journal of Laser Applications
Periodontal (gum) diseases present one of the main threats to oral and general health; they are v... more Periodontal (gum) diseases present one of the main threats to oral and general health; they are very prevalent across the globe and, if left untreated, lead to tooth loss, with a significant impact on the quality of life. In addition, the impact of gum infection and inflammation on diabetes and cardiovascular disease complications has been well documented. Having in mind the key role of bacteria and the host’s immune response in the development of periodontal diseases but also the subgingival niche anatomy that complicates the adequate management of the dental biofilm and post-treatment healing, the field of periodontics is open to new or additional therapeutic methods that would complement the conventional approaches to periodontal care. Lasers were one of the first instruments used for photon therapy in dentistry due to their ability to emit a collimated, coherent light beam with monochromatic and synchronous wavelengths. We reviewed here the possibilities of the application and t...
Frontiers in Medicine, 2021
Campylobacter jejuni (C. jejuni) is one of the most frequent causes of bacterial enterocolitis gl... more Campylobacter jejuni (C. jejuni) is one of the most frequent causes of bacterial enterocolitis globally. The disease in human is usually self-limiting, but when complications arise antibiotic therapy is required at a time when resistance to antibiotics is increasing worldwide. Mechanisms of antibiotic resistance in bacteria are diverse depending on antibiotic type and usage and include: enzymatic destruction or drug inactivation; alteration of the target enzyme; alteration of cell membrane permeability; alteration of ribosome structure and alteration of the metabolic pathway(s). Resistance of Campylobacter spp. to antibiotics, especially fluoroquinolones is now a major public health problem in developed and developing countries. In this review the mechanisms of resistance to fluoroquinolones, macrolides, tetracycline, aminoglycoside and the role of integrons in resistance of Campylobacter (especially at the molecular level) are discussed, as well as the mechanisms of resistance to β...
Timocki medicinski glasnik, 2021
The tendency to replace lost teeth by implanting foreign material is as old as civilization itsel... more The tendency to replace lost teeth by implanting foreign material is as old as civilization itself. The accelerated development of implantology as a science started only in the middle of the last century. The essence of implantology is the process of osseointegration. The greatest merits for this phenomenon belong to prof. Per Ingvar Brenemark, who accidentally discovered the possibility of complete incorporation of a titanium implant into the surrounding bone. With the discovery of osseointegration and defining the conditions that enable it, the period of implantology development begins with the improvement of endosseous implantation into an efficient method of prosthetic rehabilitation, predictable outcome and extended lifespan of implants and dental restoration in function. Implantology initially aimed to improve the function and quality of life of partial and complete edentulism patients, and since the 1990s it has become prosthetically guided not only by functional but also by ...
PONS - medicinski casopis, 2018
Metabolic syndrome is a set of numerous risk factors for the development of cardiovascular diseas... more Metabolic syndrome is a set of numerous risk factors for the development of cardiovascular disease and type 2 diabetes mellitus (DM2). Metabolic syndrome includes an amalgam of biochemical disorders: increased blood pressure, blood sugar, excess body fat around the waist, increased triglycerides and cholesterol levels. Although there are several Metabolic syndrome definitions today with different criteria for setting diagnosis, its significance is not reduced. Early detection and adequate treatment will prevent the occurrence of metabolic syndrome complications. The aim of this paper is to critically compare different criteria for diagnosing metabolic syndrome, bearing in mind that there are no unified positions for defining this for life dangerous and highly represented syndrome.
PONS - medicinski casopis, 2019
Ceramics International, 2021
Abstract The purpose of this study was to investigate the influence of different radiopacifiers o... more Abstract The purpose of this study was to investigate the influence of different radiopacifiers on the physicochemical and biological properties of novel calcium silicate based endodontic ceramic enriched with bioactive nano-particulated hydroxyapatite – ECHA. Namely, ECHA was used as a basis for mixing with the following radiopacifiers: strontium fluoride (SrF2), zirconium dioxide (ZrO2) and bismuth oxide (Bi2O3). For comparison, Portland cement (PC) and mineral trioxide aggregate (MTA) were used. The following physicochemical characteristics were examined: the radiopacity, setting time, compressive strength, porosity, wettability and pH value. The biocompatibility of the cements was assessed by crystal violet, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) and cell adhesion assays. The highest radiopacity was obtained for the ECHA + Bi2O3 mixture and MTA that were statistically significant in comparison to other materials (p 0.05). Experimental cements exhibited statistically higher contact angles of glycerol than PC and MTA (p
Iranian Journal of Public Health
Background: Elevated blood pressure (BP) is one of the leading causes for developing major cardio... more Background: Elevated blood pressure (BP) is one of the leading causes for developing major cardiovascular events and still represents a major public health challenge worldwide. We aimed to provide data on predictors of poor adherence to medication in hypertensive patients in Serbia. Methods: Observational, analytical study was undertaken at a group of 388 patients who refilled their medications in the Pharmacy Institution, “Apoteka Kragujevac”, Kragujevac, Serbia between Jan and Mar 2019. Afterward, we conducted a case-control study to evaluate the influence of the variables associated with the adherence. We used a self-developed questionnaire and SF-36 to assess the influence of the quality of life on medication adherence. Results: Results revealed four independent predictors of non-adherence: increased number of medications, living in a city, forgetfulness of the dosing regimen and low energy. The odds of non-adherence were the highest among the participants living in the city and...
Serbian Dental Journal
Introduction. Implant placement with simultaneous bone augmentation presents procedure with high ... more Introduction. Implant placement with simultaneous bone augmentation presents procedure with high risk of complications. In those situations, one of the surgical procedures with promising outcomes is the split crest technique. Case report. A 69-year-old edentulous patient was referred to the Clinic of Oral Surgery, at the Faculty of Dentistry in Pancevo. The width of the mandibular alveolar ridge in the inter-canine region was 2 mm. For the ridge splitting and expansion, a special split and expansion kit was used (Esset KitR, Osstem, South Korea). The procedure started with crestal remover carbide cylindrical bur of 7 mm diameter for flattening of the alveolar ridge to the width of 4 mm. Then, a sharp lance drill was used to mark the places for implant osteotomies and placement. After that, 1.8 mm twist drill was used to prepare a bed of 10 mm depth, and a 13 mm saw was directed vertically. Set of expansion drills were used to expand the alveolar ridge gradually. Two implants 3.5x10 ...
Vojnosanitetski pregled, 2021
Background/Aim. The materials used nowadays for bone replacement do not fully meet the requiremen... more Background/Aim. The materials used nowadays for bone replacement do not fully meet the requirements for complete regeneration, which is why new ones are being tested. Despite numerous attempts to improve bone tissue regeneration, no fulfilling material has been found yet. This study investigated the influence of poly-lactide-co-glycolide (PLGA) and polyethyleneimine (PEI) as coatings for hydroxyapatite (HAP) bone carriers on bone tissue regenerative potential in rabbits? calvarial defect. Methods. Calvarial defects measuring 6 mm in diameter were made in 19 skeletally mature rabbits. Defects were filled with one of the following materials: PLGA coated HAP (HAP + PLGA), PEI coated HAP (HAP + PEI), and bovine HAP ? Bio-Oss? (positive control). Unfilled defects represented negative control. Histological analysis was performed in order to determine the inflammatory response of the host tissue. The formation of the new bone was evaluated using histomorphometric analysis. All analyses hav...
International Journal of Environmental Research and Public Health
The COVID-19 pandemic has drastically changed the routine way of life, having consequences in man... more The COVID-19 pandemic has drastically changed the routine way of life, having consequences in many segments of life, including dental practice and education. The aim of this study was to evaluate the frequency of probable bruxism in a sample of dental students in Serbia and to estimate the potential association between psychological factors related to the COVID-19 pandemic and the presence of bruxism. A cross-sectional study included 178 dental students in Serbia, who were interviewed using a specially-designed self-administered online questionnaire, which consisted of three sections, and after that, a clinical examination for the presence of bruxism symptoms in the oral cavity. Psychological status was evaluated using the Depression, Anxiety and Stress Scale-21 (DASS-21) and the Fear of COVID-19 Scale (FCV-19S). Saliva samples were taken to analyze salivary cortisol levels. The prevalence of probable bruxism was 34.8%. Respondents with probable bruxism had significantly higher DASS...
European Journal of Dental and Oral Health
The presence of supernumerary teeth is one of the most common factors that can alter the normal e... more The presence of supernumerary teeth is one of the most common factors that can alter the normal eruption pattern. We present here a rare phenomenon of four, molariform mesiodentes in a systemically healthy, non-syndromic patient, with non-contributory family history, causing delayed eruption of permanent, upper, central incisors. Multiple supernumerary teeth can be an important component of distinctive disorders and a valuable clue for their early diagnosis. However, this case highlights the importance of non-hereditary factors in the pathogenesis of multiple supernumerary teeth in a non-syndromic patient.
Serbian Dental Journal, 2020
Sodium hypochlorite (NaOCl) is a strong antiseptic agent that is usually used as endodontic irrig... more Sodium hypochlorite (NaOCl) is a strong antiseptic agent that is usually used as endodontic irrigant for dissolving organic parts of pulp and dentin and neutralizing toxic products. However, the use of sodium hypochlorite may cause destruction of blood vessels, soft tissues and bone necrosis if extruded into the periapical region. Urgent treatment of the NaOCl accident should be oriented on pain relief, infection control and recovery. This paper shows a case of periradicular tissue necrosis after accidental extrusion on NaOCl through fausse route of right maxillary canine, and complete tissue regeneration after surgical debridement and bone augmentation with addition of platelet rich fibrin (PRF) that occurred in a 53-year old patient at the dental clinic of Faculty of Dentistry in Pancevo.
Introduction: Intentional partial odontectomy or coronectomy is primarily advocated in situations... more Introduction: Intentional partial odontectomy or coronectomy is primarily advocated in situations where there are intimate relationships between the roots of the tooth indicated for extraction and the inferior alveolar nerve (IAN). The aim of this report is to present a patient having two neighbouring infra-occluded teeth in the right mandible indicated for extraction prior to prosthetic rehabilitation, which were coronectomied as to prevent injuring of the IAN and causing iatrogenic fracture of the mandibular body. Patient’s report: Coronectomy of both teeth was performed as recommended in literature, and the patient did not have any special discomfort after the operation or deficit in sensitive nerve function, and the wound healed uneventfully. The patient was followed regularly during a two- year’s follow-up. Conclusion: In this patient, coronectomy appeared quite beneficial solution for avoiding serious problems (injuring of the IAN and possible fracture of the mandible) and mak...
Serbian Journal of Experimental and Clinical Research
Precise estimation of life quality is of special importance in patients with chronic diseases, su... more Precise estimation of life quality is of special importance in patients with chronic diseases, such as arterial hypertension. There are many questionnaires for that purpose. SF 36 with 8 domains has been proved as one of the most appropriate. To date, there was no translated and validated SF 36 in the Serbian language for hypertensive patients. The aim of this study was to test validity and reliability of the SF-36 in Serbian patients with diagnosed arterial hypertension. Cronbach’s alpha coefficient was calculated to assess the internal consistency of the Serbian version of the SF-36. After deducting the overlap between each of the 36 items and its related domain, the collective validity was considered to be good if the correlation coefficient remains > 0.4. Only 2.54% answers to the questions were missing. The values of all 8 domains were higher in men than in women. Cronbach alpha coefficient was high for the SF-36, 0.897, and it suggests that the SF-36 had good internal relia...
Clinical Oral Investigations, 2013
Journal of endodontics, Jan 12, 2018
The outcome of periapical surgery has been directly improved with the introduction of novel mater... more The outcome of periapical surgery has been directly improved with the introduction of novel material formulations. The aim of the study was to compare the retrograde obturation quality of the following materials: calcium silicate (Biodentine; Septodont, Saint-Maur-des-Fosses, France), mineral trioxide aggregate (MTA+; Cerkamed Company, Stalowa Wola, Poland), and glass ionomer cement (Fuji IX; GC Corporation, Tokyo, Japan). Materials' wettability was calculated concerning the contact angles of the cements measured using a glycerol drop. Cements' porosity was determined using mercury intrusion porosimetry and micro-computed tomographic (μCT) imaging. Extracted upper human incisors were retrofilled, and μCT analysis was applied to calculate the volume of the gap between the retrograde filling material and root canal dentin. Experiments were performed before and after soaking the materials in simulated body fluid (SBF). No statistically significant differences were found among t...
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Papers by Vladimir Biocanin