Antibiotic resistance or microbial drug resistance is emerging as a serious threat to human healt... more Antibiotic resistance or microbial drug resistance is emerging as a serious threat to human healthcare globally, and the multidrug-resistant (MDR) strains are imposing major hurdles to the progression of drug discovery programs. Newer antibiotic-resistance mechanisms in microbes contribute to the inefficacy of the existing drugs along with the prolonged illness and escalating expenditures. The injudicious usage of the conventional and commonly available antibiotics in human health, hygiene, veterinary and agricultural practices is proving to be a major driver for evolution, persistence and spread of antibiotic-resistance at a frightening rate. The drying pipeline of new and potent antibiotics is adding to the severity. Therefore, novel and effective new drugs and innovative therapies to treat MDR infections are urgently needed. Apart from the different natural and synthetic drugs being tested, plant secondary metabolites or phytochemicals are proving efficient in combating the drug-...
The viscous fluid flow past a semi-infinite porous solid, which is proportionally sheared at one ... more The viscous fluid flow past a semi-infinite porous solid, which is proportionally sheared at one boundary with the possibility of the fluid slipping according to Navier’s slip or second order slip, is considered here. Such an assumption takes into consideration several of the boundary conditions used in the literature, and is a generalization of them. Upon introducing a similarity transformation, the governing equations for the problem under consideration reduces to a system of nonlinear partial differential equations. Interestingly, we were able to obtain an exact analytical solution for the velocity, though the equation is nonlinear. The flow through the porous solid is assumed to obey the Brinkman equation, and is considered relevant to several applications.
The aim of this study was to determine whether intra-oral de novo regenerated mucosa (D) that gre... more The aim of this study was to determine whether intra-oral de novo regenerated mucosa (D) that grew over free fibula flap reconstructed-mandibles resembled the donor tissue i.e. external skin (S) of the lateral leg, or the recipient site tissue, i.e. keratinized oral mucosa (K). Differential proteome analysis was performed with ten tissue samples from each of the three groups: de novo regenerated mucosa (D), external skin (S), and keratinized oral mucosa (K). Expression differences of cornulin and involucrin were validated by Western blot analysis and their spatial distributions in the respective tissues were ascertained by immunohistochemistry. From all three investigated tissue types a total of 1188 proteins were identified, 930 of which were reproducibly and robustly quantified by proteome analysis. The best differentiating proteins were assembled in an oral mucosa proteome signature that encompasses 56 differentially expressed proteins. Principal component analysis of both, the 9...
Oral cancer is the most common type of cancer among men in India and other countries in South Asi... more Oral cancer is the most common type of cancer among men in India and other countries in South Asia. Late diagnosis contributes significantly to this mortality, highlighting the need for effective and specific point-of-care diagnostic tools. The same regions with high prevalence of oral cancer have seen extensive growth in mobile phone infrastructure, which enables widespread access to telemedicine services. In this work, we describe the evaluation of an automated tablet-based mobile microscope as an adjunct for telemedicine-based oral cancer screening in India. Brush biopsy, a minimally invasive sampling technique was combined with a simplified staining protocol and a tablet-based mobile microscope to facilitate local collection of digital images and remote evaluation of the images by clinicians. The tablet-based mobile microscope (CellScope device) combines an iPad Mini with collection optics, LED illumination and Bluetooth-controlled motors to scan a slide specimen and capture hig...
The purpose of this study was to evaluate the anesthetic efficiency of local infiltration anesthe... more The purpose of this study was to evaluate the anesthetic efficiency of local infiltration anesthesia administered with a pressure syringe (P-INF) via a special technique versus direct block anesthesia of the inferior alveolar nerve (IANB) for tooth extraction in the posterior mandible. In a prospective randomized study, 101 teeth in 101 patients were extracted in the posterior mandible under local anesthesia whereby two different administration techniques were used (P-INF n = 48; IANB n = 53). Primary objectives were comparisons of anesthetic success rate (yes/no) and efficacy (full/sufficient vs. insufficient). Secondary objectives were patients' pain perception during treatment, pain of injection (numerical rating scale), need for second injections (always IANB), time until onset of anesthetic action (min), and duration of local numbness (min). IANB was successful in all cases, whereas initial P-INF achieved 35% of success only. Furthermore, IANB reached significant higher val...
International Journal of Modern Education and Computer Science
Mathematics is universally accepted as mother of all science. Despite that, Department of Personn... more Mathematics is universally accepted as mother of all science. Despite that, Department of Personnel and Training (DOPT) has recently issued a circular mentioning that a person having master degree in mathematics cannot be considered for the post of scientists. The open question of ‗Is mathematics a science?' is revisited in this paper under the new perspective to explore scientific practices that sans mathematics arrived knocking, challenging basic understanding of precision and practical sense that makes science. Considering the fact that in India, most crucial policy decisions at a higher level of abstraction in every conceivable arena of our national life are taken by either GOM (Group of Ministers) or GOS (Group of Secretaries), apprehension raises a basic query 'Who decides?' Some decision causes much unexpected consequence, which is noticed when it takes its toll and becomes virtually irreversible. This recent decision of Flexible Complementing Scheme (FCS), wherein mathematics is not considered as science, has potential to damage the very scientific culture and practices in India. This paper is an attempt to place mathematics in its right perspective and to highlight the damage that this decision might do. The paper also suggests ways to control the damage.
Effective chemoprevention is critical for improving outcomes of oral cancer. As single agents, cu... more Effective chemoprevention is critical for improving outcomes of oral cancer. As single agents, curcumin and metformin, are reported to exhibit chemopreventive properties, in vitro as well as in patients with oral cancer. In this study, the chemopreventive efficacy of this drug combination was tested in a 4NQO (4-nitro-quinoline-oxide) induced, mice oral carcinogenesis model. Molecular analysis revealed a cancer stem cell (CSC)-driven, oral carcinogenic progression in this model, wherein a progressive increase in the expression of CSC-specific markers (CD44 and CD133) was observed from 8th to 25th week, at transcript (40 to 100 fold) and protein levels (p≤0.0001). Chemopreventive treatment of the animals at 17th week with curcumin and metformin, indicated that the combination regimen decreased tumor volume when compared to the control arm (0.69 + 0.03 vs 6.66 + 2.4mm(3) ; p = 0.04) and improved overall survival of the animals (p = 0.03). Assessment of the molecular status showed an o...
Following publication of this article [1], it has come to our attention that an incomplete versio... more Following publication of this article [1], it has come to our attention that an incomplete version of Fig. 7 was included in this article. The complete figure is shown below with the missing text included to the left of the chart.
Modeling of microbial metabolism is a topic of growing importance in biotechnology. Mathematical ... more Modeling of microbial metabolism is a topic of growing importance in biotechnology. Mathematical modeling helps provide a mechanistic understanding for the studied process, separating the main drivers from the circumstantial ones, bounding the outcomes of experiments and guiding engineering approaches. Among different modeling schemes, the quantification of intracellular metabolic fluxes (i.e. the rate of each reaction in cellular metabolism) is of particular interest for metabolic engineering because it describes how carbon and energy flow throughout the cell. In addition to flux analysis, new methods for the effective use of the ever more readily available and abundant -omics data (i.e. transcriptomics, proteomics and metabolomics) are urgently needed. The jQMM library presented here provides an open-source, Python-based framework for modeling internal metabolic fluxes and leveraging other -omics data for the scientific study of cellular metabolism and bioengineering purposes. Fir...
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, Jan 30, 2016
Aim of the study was to assess the role of angiogenesis in the process of malignant transformatio... more Aim of the study was to assess the role of angiogenesis in the process of malignant transformation of clinical diagnosed oral leukoplakia (OL). 131 histological preparations (oral leukoplakia/hyperkeratosis without dysplasia (OL; n=49), oral leukoplakia/hyperkeratosis with mild dysplasia (OL-SIN1; n=33), with moderate dysplasia (OL-SIN2; n=13) and leukoplakia-derived oral squamous cell carcinoma (OL-OSCC; n=36)) were evaluated for microvessel density (MVD), vessel diameter as well as for vascular endothelial growth factor (VEGF-A) expression. Data were compared within the groups. For MVD, there were significant differences between OL and OL-SIN 2/OL-OSCC (P < 0.05) and between OL-SIN 1 and OL-OSCC (P < 0.05). For OL-OSCC, vessel diameters were significantly increased compared to OL (P < 0.05). Expression of VEGF-A increased significantly gradually from OL-SIN 1 to OSCC (each P < 0.05). This was especially evident for lesions of the tongue when compared to the others. Ang...
Background: Establishing individual identification of a decedent only by dental means is a mammot... more Background: Establishing individual identification of a decedent only by dental means is a mammoth task in forensic odontology. Palatal rugae's uniqueness, its resistance to heat, and stability throughout life have been proved by its use as an alternative aid in individual identification where comparison of fingerprints and other records is difficult. Aims and Objectives: The aim of the present study was to analyze the role of palatal rugoscopy in personal identification and sex determination of Nalgonda pediatric population. Materials and Methods: The study group consisted of 100 children having mixed dentition within the age range of 8-11 years, residing in Nalgonda district. Palatal rugae pattern, shape of the incisive papillae, length of the median palatal raphae, and shape of the dental arches were analyzed using Chi-square and Mann-Whitney tests between males and females. Results: Wavy and curved patterns appeared to be most prevalent in both males and females but with no significant difference. The number of primary rugae in females and secondary rugae in males, on left side of the palate, was significantly more than their counterparts (P < 0.05). When rugae unification was observed, diverging type was significantly more in males than in females. Parabolic dental arch form, elliptical type of incisive papilla, and medium length of median palatal raphae was observed in majority of the subjects. Conclusion: The present study hypothesizes the uniqueness of the rugae in personal identification as no two palates showed similar type of rugae in either of the genders. The rugae pattern also contributes minimally towards sex determination as there was no significant difference observed between the two variables.
In patients with reconstructed mandibles using free fibula flaps, management of soft tissues arou... more In patients with reconstructed mandibles using free fibula flaps, management of soft tissues around implants supporting dental rehabilitation, is often a clinical problem. The aim of this paper is to describe a new technique, namely &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;Sub-periosteal dissection and denture-guided epithelial regeneration (SD-DGER)&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;, as a method of peri-implant soft tissue management in these patients. The technique consists of performing a subperiosteal dissection with creation of buccal and lingual flaps. These flaps form the buccal and lingual vestibule. Implants are placed and an interim denture is immediately loaded onto the implants to guide the regenerated epithelium. A keratinized mucosal layer is formed on the bare fibula bone in six months time. This technique was successful in producing fixed keratinized epithelial tissue around implants in patients with mandibles reconstructed using the free fibula flap in patients who did not undergo radiotherapy. The sub-periosteal dissection with denture guided epithelial regeneration is a predictable form of peri-implant soft tissue management in selected patients with reconstructed jaws.
Fetal liver-and thymus-derived NK1.1 ؉ cells do not express known Ly-49 receptors. Despite the ab... more Fetal liver-and thymus-derived NK1.1 ؉ cells do not express known Ly-49 receptors. Despite the absence of Ly-49 inhibitory receptors, fetal and neonatal NK1.1 ؉ Ly-49 ؊ cells can distinguish between class I high and class I low target cells, suggesting the existence of other class I-specific inhibitory receptors. We demonstrate that fetal NK1.1 ؉ Ly-49 ؊ cell lysates contain CD94 protein and that a significant proportion of fetal NK cells are bound by Qa1 b tetramers. Fetal and adult NK cells efficiently lyse lymphoblasts from K b؊/؊ D b؊/؊ mice. Qa1 bspecific peptides Qdm and HLA-CW4 leader peptide specifically inhibited the lysis of these blasts by adult and fetal NK cells. Qdm peptide also inhibited the lysis of Qa1 b-transfected human 721.221 cells by fetal NK cells. Taken together, these results suggest that the CD94/NKG2A receptor complex is the major known inhibitory receptor for class I (Qa1 b) molecules on developing fetal NK cells.
Current limitations in quantitatively predicting biological behavior hinder our efforts to engine... more Current limitations in quantitatively predicting biological behavior hinder our efforts to engineer biological systems to produce biofuels and other desired chemicals. Here, we present a new method for calculating metabolic fluxes, key targets in metabolic engineering, that incorporates data from 13C labeling experiments and genome-scale models. The data from 13C labeling experiments provide strong flux constraints that eliminate the need to assume an evolutionary optimization principle such as the growth rate optimization assumption used in Flux Balance Analysis (FBA). This effective constraining is achieved by making the simple but biologically relevant assumption that flux flows from core to peripheral metabolism and does not flow back. The new method is significantly more robust than FBA with respect to errors in genome-scale model reconstruction. Furthermore, it can provide a comprehensive picture of metabolite balancing and predictions for unmeasured extracellular fluxes as co...
The aim of this clinical paper is to introduce a technique to plan for functional maxillofacial r... more The aim of this clinical paper is to introduce a technique to plan for functional maxillofacial reconstructions. Preoperative dental casts were made of the patient and mock surgery performed on the casts. A fibula analogue was then placed in an ideal functional reconstruction position. New dentures were fabricated on the fibula analogue and drill holes for the placement of implants were placed through the denture. This denture formed as a guide to position the fibula transplant during surgery. This technique was useful in producing functional and rehabilitative outcomes in cases of both maxillary and mandibular reconstructive surgeries. The Jugaad technique-denture based inverse planning-is a cost effective method for planning and executing maxillofacial reconstructions using mock surgery on casts and interim dentures.
Antibiotic resistance or microbial drug resistance is emerging as a serious threat to human healt... more Antibiotic resistance or microbial drug resistance is emerging as a serious threat to human healthcare globally, and the multidrug-resistant (MDR) strains are imposing major hurdles to the progression of drug discovery programs. Newer antibiotic-resistance mechanisms in microbes contribute to the inefficacy of the existing drugs along with the prolonged illness and escalating expenditures. The injudicious usage of the conventional and commonly available antibiotics in human health, hygiene, veterinary and agricultural practices is proving to be a major driver for evolution, persistence and spread of antibiotic-resistance at a frightening rate. The drying pipeline of new and potent antibiotics is adding to the severity. Therefore, novel and effective new drugs and innovative therapies to treat MDR infections are urgently needed. Apart from the different natural and synthetic drugs being tested, plant secondary metabolites or phytochemicals are proving efficient in combating the drug-...
The viscous fluid flow past a semi-infinite porous solid, which is proportionally sheared at one ... more The viscous fluid flow past a semi-infinite porous solid, which is proportionally sheared at one boundary with the possibility of the fluid slipping according to Navier’s slip or second order slip, is considered here. Such an assumption takes into consideration several of the boundary conditions used in the literature, and is a generalization of them. Upon introducing a similarity transformation, the governing equations for the problem under consideration reduces to a system of nonlinear partial differential equations. Interestingly, we were able to obtain an exact analytical solution for the velocity, though the equation is nonlinear. The flow through the porous solid is assumed to obey the Brinkman equation, and is considered relevant to several applications.
The aim of this study was to determine whether intra-oral de novo regenerated mucosa (D) that gre... more The aim of this study was to determine whether intra-oral de novo regenerated mucosa (D) that grew over free fibula flap reconstructed-mandibles resembled the donor tissue i.e. external skin (S) of the lateral leg, or the recipient site tissue, i.e. keratinized oral mucosa (K). Differential proteome analysis was performed with ten tissue samples from each of the three groups: de novo regenerated mucosa (D), external skin (S), and keratinized oral mucosa (K). Expression differences of cornulin and involucrin were validated by Western blot analysis and their spatial distributions in the respective tissues were ascertained by immunohistochemistry. From all three investigated tissue types a total of 1188 proteins were identified, 930 of which were reproducibly and robustly quantified by proteome analysis. The best differentiating proteins were assembled in an oral mucosa proteome signature that encompasses 56 differentially expressed proteins. Principal component analysis of both, the 9...
Oral cancer is the most common type of cancer among men in India and other countries in South Asi... more Oral cancer is the most common type of cancer among men in India and other countries in South Asia. Late diagnosis contributes significantly to this mortality, highlighting the need for effective and specific point-of-care diagnostic tools. The same regions with high prevalence of oral cancer have seen extensive growth in mobile phone infrastructure, which enables widespread access to telemedicine services. In this work, we describe the evaluation of an automated tablet-based mobile microscope as an adjunct for telemedicine-based oral cancer screening in India. Brush biopsy, a minimally invasive sampling technique was combined with a simplified staining protocol and a tablet-based mobile microscope to facilitate local collection of digital images and remote evaluation of the images by clinicians. The tablet-based mobile microscope (CellScope device) combines an iPad Mini with collection optics, LED illumination and Bluetooth-controlled motors to scan a slide specimen and capture hig...
The purpose of this study was to evaluate the anesthetic efficiency of local infiltration anesthe... more The purpose of this study was to evaluate the anesthetic efficiency of local infiltration anesthesia administered with a pressure syringe (P-INF) via a special technique versus direct block anesthesia of the inferior alveolar nerve (IANB) for tooth extraction in the posterior mandible. In a prospective randomized study, 101 teeth in 101 patients were extracted in the posterior mandible under local anesthesia whereby two different administration techniques were used (P-INF n = 48; IANB n = 53). Primary objectives were comparisons of anesthetic success rate (yes/no) and efficacy (full/sufficient vs. insufficient). Secondary objectives were patients' pain perception during treatment, pain of injection (numerical rating scale), need for second injections (always IANB), time until onset of anesthetic action (min), and duration of local numbness (min). IANB was successful in all cases, whereas initial P-INF achieved 35% of success only. Furthermore, IANB reached significant higher val...
International Journal of Modern Education and Computer Science
Mathematics is universally accepted as mother of all science. Despite that, Department of Personn... more Mathematics is universally accepted as mother of all science. Despite that, Department of Personnel and Training (DOPT) has recently issued a circular mentioning that a person having master degree in mathematics cannot be considered for the post of scientists. The open question of ‗Is mathematics a science?' is revisited in this paper under the new perspective to explore scientific practices that sans mathematics arrived knocking, challenging basic understanding of precision and practical sense that makes science. Considering the fact that in India, most crucial policy decisions at a higher level of abstraction in every conceivable arena of our national life are taken by either GOM (Group of Ministers) or GOS (Group of Secretaries), apprehension raises a basic query 'Who decides?' Some decision causes much unexpected consequence, which is noticed when it takes its toll and becomes virtually irreversible. This recent decision of Flexible Complementing Scheme (FCS), wherein mathematics is not considered as science, has potential to damage the very scientific culture and practices in India. This paper is an attempt to place mathematics in its right perspective and to highlight the damage that this decision might do. The paper also suggests ways to control the damage.
Effective chemoprevention is critical for improving outcomes of oral cancer. As single agents, cu... more Effective chemoprevention is critical for improving outcomes of oral cancer. As single agents, curcumin and metformin, are reported to exhibit chemopreventive properties, in vitro as well as in patients with oral cancer. In this study, the chemopreventive efficacy of this drug combination was tested in a 4NQO (4-nitro-quinoline-oxide) induced, mice oral carcinogenesis model. Molecular analysis revealed a cancer stem cell (CSC)-driven, oral carcinogenic progression in this model, wherein a progressive increase in the expression of CSC-specific markers (CD44 and CD133) was observed from 8th to 25th week, at transcript (40 to 100 fold) and protein levels (p≤0.0001). Chemopreventive treatment of the animals at 17th week with curcumin and metformin, indicated that the combination regimen decreased tumor volume when compared to the control arm (0.69 + 0.03 vs 6.66 + 2.4mm(3) ; p = 0.04) and improved overall survival of the animals (p = 0.03). Assessment of the molecular status showed an o...
Following publication of this article [1], it has come to our attention that an incomplete versio... more Following publication of this article [1], it has come to our attention that an incomplete version of Fig. 7 was included in this article. The complete figure is shown below with the missing text included to the left of the chart.
Modeling of microbial metabolism is a topic of growing importance in biotechnology. Mathematical ... more Modeling of microbial metabolism is a topic of growing importance in biotechnology. Mathematical modeling helps provide a mechanistic understanding for the studied process, separating the main drivers from the circumstantial ones, bounding the outcomes of experiments and guiding engineering approaches. Among different modeling schemes, the quantification of intracellular metabolic fluxes (i.e. the rate of each reaction in cellular metabolism) is of particular interest for metabolic engineering because it describes how carbon and energy flow throughout the cell. In addition to flux analysis, new methods for the effective use of the ever more readily available and abundant -omics data (i.e. transcriptomics, proteomics and metabolomics) are urgently needed. The jQMM library presented here provides an open-source, Python-based framework for modeling internal metabolic fluxes and leveraging other -omics data for the scientific study of cellular metabolism and bioengineering purposes. Fir...
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, Jan 30, 2016
Aim of the study was to assess the role of angiogenesis in the process of malignant transformatio... more Aim of the study was to assess the role of angiogenesis in the process of malignant transformation of clinical diagnosed oral leukoplakia (OL). 131 histological preparations (oral leukoplakia/hyperkeratosis without dysplasia (OL; n=49), oral leukoplakia/hyperkeratosis with mild dysplasia (OL-SIN1; n=33), with moderate dysplasia (OL-SIN2; n=13) and leukoplakia-derived oral squamous cell carcinoma (OL-OSCC; n=36)) were evaluated for microvessel density (MVD), vessel diameter as well as for vascular endothelial growth factor (VEGF-A) expression. Data were compared within the groups. For MVD, there were significant differences between OL and OL-SIN 2/OL-OSCC (P < 0.05) and between OL-SIN 1 and OL-OSCC (P < 0.05). For OL-OSCC, vessel diameters were significantly increased compared to OL (P < 0.05). Expression of VEGF-A increased significantly gradually from OL-SIN 1 to OSCC (each P < 0.05). This was especially evident for lesions of the tongue when compared to the others. Ang...
Background: Establishing individual identification of a decedent only by dental means is a mammot... more Background: Establishing individual identification of a decedent only by dental means is a mammoth task in forensic odontology. Palatal rugae's uniqueness, its resistance to heat, and stability throughout life have been proved by its use as an alternative aid in individual identification where comparison of fingerprints and other records is difficult. Aims and Objectives: The aim of the present study was to analyze the role of palatal rugoscopy in personal identification and sex determination of Nalgonda pediatric population. Materials and Methods: The study group consisted of 100 children having mixed dentition within the age range of 8-11 years, residing in Nalgonda district. Palatal rugae pattern, shape of the incisive papillae, length of the median palatal raphae, and shape of the dental arches were analyzed using Chi-square and Mann-Whitney tests between males and females. Results: Wavy and curved patterns appeared to be most prevalent in both males and females but with no significant difference. The number of primary rugae in females and secondary rugae in males, on left side of the palate, was significantly more than their counterparts (P < 0.05). When rugae unification was observed, diverging type was significantly more in males than in females. Parabolic dental arch form, elliptical type of incisive papilla, and medium length of median palatal raphae was observed in majority of the subjects. Conclusion: The present study hypothesizes the uniqueness of the rugae in personal identification as no two palates showed similar type of rugae in either of the genders. The rugae pattern also contributes minimally towards sex determination as there was no significant difference observed between the two variables.
In patients with reconstructed mandibles using free fibula flaps, management of soft tissues arou... more In patients with reconstructed mandibles using free fibula flaps, management of soft tissues around implants supporting dental rehabilitation, is often a clinical problem. The aim of this paper is to describe a new technique, namely &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;Sub-periosteal dissection and denture-guided epithelial regeneration (SD-DGER)&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;quot;, as a method of peri-implant soft tissue management in these patients. The technique consists of performing a subperiosteal dissection with creation of buccal and lingual flaps. These flaps form the buccal and lingual vestibule. Implants are placed and an interim denture is immediately loaded onto the implants to guide the regenerated epithelium. A keratinized mucosal layer is formed on the bare fibula bone in six months time. This technique was successful in producing fixed keratinized epithelial tissue around implants in patients with mandibles reconstructed using the free fibula flap in patients who did not undergo radiotherapy. The sub-periosteal dissection with denture guided epithelial regeneration is a predictable form of peri-implant soft tissue management in selected patients with reconstructed jaws.
Fetal liver-and thymus-derived NK1.1 ؉ cells do not express known Ly-49 receptors. Despite the ab... more Fetal liver-and thymus-derived NK1.1 ؉ cells do not express known Ly-49 receptors. Despite the absence of Ly-49 inhibitory receptors, fetal and neonatal NK1.1 ؉ Ly-49 ؊ cells can distinguish between class I high and class I low target cells, suggesting the existence of other class I-specific inhibitory receptors. We demonstrate that fetal NK1.1 ؉ Ly-49 ؊ cell lysates contain CD94 protein and that a significant proportion of fetal NK cells are bound by Qa1 b tetramers. Fetal and adult NK cells efficiently lyse lymphoblasts from K b؊/؊ D b؊/؊ mice. Qa1 bspecific peptides Qdm and HLA-CW4 leader peptide specifically inhibited the lysis of these blasts by adult and fetal NK cells. Qdm peptide also inhibited the lysis of Qa1 b-transfected human 721.221 cells by fetal NK cells. Taken together, these results suggest that the CD94/NKG2A receptor complex is the major known inhibitory receptor for class I (Qa1 b) molecules on developing fetal NK cells.
Current limitations in quantitatively predicting biological behavior hinder our efforts to engine... more Current limitations in quantitatively predicting biological behavior hinder our efforts to engineer biological systems to produce biofuels and other desired chemicals. Here, we present a new method for calculating metabolic fluxes, key targets in metabolic engineering, that incorporates data from 13C labeling experiments and genome-scale models. The data from 13C labeling experiments provide strong flux constraints that eliminate the need to assume an evolutionary optimization principle such as the growth rate optimization assumption used in Flux Balance Analysis (FBA). This effective constraining is achieved by making the simple but biologically relevant assumption that flux flows from core to peripheral metabolism and does not flow back. The new method is significantly more robust than FBA with respect to errors in genome-scale model reconstruction. Furthermore, it can provide a comprehensive picture of metabolite balancing and predictions for unmeasured extracellular fluxes as co...
The aim of this clinical paper is to introduce a technique to plan for functional maxillofacial r... more The aim of this clinical paper is to introduce a technique to plan for functional maxillofacial reconstructions. Preoperative dental casts were made of the patient and mock surgery performed on the casts. A fibula analogue was then placed in an ideal functional reconstruction position. New dentures were fabricated on the fibula analogue and drill holes for the placement of implants were placed through the denture. This denture formed as a guide to position the fibula transplant during surgery. This technique was useful in producing functional and rehabilitative outcomes in cases of both maxillary and mandibular reconstructive surgeries. The Jugaad technique-denture based inverse planning-is a cost effective method for planning and executing maxillofacial reconstructions using mock surgery on casts and interim dentures.
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