Background 1,3-b-glucanases (group 2 of pathogenesis-related proteins) are enzymes widely distrib... more Background 1,3-b-glucanases (group 2 of pathogenesis-related proteins) are enzymes widely distributed among higher plants and have been recently proven to be significant allergens. Objective The aim of this work was to study the potential implication of 1,3-b-glucanases in crossreactivities among latex, pollen and vegetable foods. Methods The cDNA encoding the N-terminal domain (NtD) of Ole e 9, a major allergenic 1,3-bglucanase from olive pollen, was amplified by polymerase chain reaction and produced as a recombinant protein in Pichia pastoris (recombinant N-terminal domain, rNtD). Circular dichroism, ELISA, immunoblotting and immunoblotting inhibition experiments were carried out. Sera from olive pollen allergic patients and a rNtD-specific polyclonal antiserum were used. Results The NtD of Ole e 9 has been produced at high yield in the yeast P. pastoris and possesses 1,3-b-glucanase activity. The expressed polypeptide conserves IgE and IgG immunodominant epitopes of the whole Ole e 9. A rNtD-specific polyclonal antiserum and sera from olive pollen allergic patients allowed detection of IgG and IgE reactive peptidic epitopes common to 1,3-bglucanase Ole e 9 in extracts from ash and birch pollen, tomato, potato, bell-pepper, banana and latex. Conclusion rNtD and homologous glucanases are new molecules to be used in diagnostic protocols as they could help to identify allergic pollen patients who are at risk for developing allergic symptoms to fruits, vegetables and latex.
First measurements of dihadron correlations for charged particles are presented for central PbPb ... more First measurements of dihadron correlations for charged particles are presented for central PbPb collisions at a nucleon-nucleon center-of-mass energy of 2.76 TeV over a broad range in relative pseudorapidity (∆η) and the full range of relative azimuthal angle (∆φ). The data were collected with the CMS detector, at the LHC. A broadening of the away-side (∆φ ≈ π) azimuthal correlation is observed at all ∆η, as compared to the measurements in pp collisions. Furthermore, long-range dihadron correlations in ∆η are observed for particles with similar φ values. This phenomenon, also known as the "ridge", persists up to at least |∆η| = 4. For particles with transverse momenta
18 / Abstract / 19 / / 20 / The crystal structure of nitropentaamminecobalt(III) nitroprusside se... more 18 / Abstract / 19 / / 20 / The crystal structure of nitropentaamminecobalt(III) nitroprusside sesquihydrate, [Co(NH 3 ) 5 NO 2 ][Fe(CN) 5 NO] ×/1.5H 2 O has / 21 / been determined by Patterson and Fourier methods employing 3881 independent X-ray reflections with I /2s (I ) and refined by / 22 / full-matrix least-squares to R 1 0/0.032. The substance crystallizes in the monoclinic C 2/c (C 2h 6 ) space group No. 15, with a 0/ / 23 / 28.408(3), b 0/6.422(1), c 0/22.401(3) Å , b 0/123.55(1)8 and Z0/8. The metastable state I (MSI) of the nitroprusside anion and the / 24 / photochemical isomerization reaction of the nitropentaamminecobalt(III) cation to the nitrito isomer were produced by irradiation / 25 / with Ar ' laser light of 488 nm wavelength at 77 K. TGA and DTA of the non-excited substance were performed under nitrogen / 26 / atmosphere. # 2002 Published by Elsevier Science Ltd. / 27 / Keywords: PPPPentacyanonitrosylferrate(2(/) ion; Metastable excited states; X-ray diffraction; FIRRS; TGA; DTA / 28 / 1. Introduction / 29 / An extensive work on nitroprussides, which includes / 30 / alkaline, alkaline-earth, and transition metals salts has / 31 / been previously performed ([1 Á/10] and references / 32 / therein). These studies provided interesting results, / 33 / especially about the vibrational and photophysical NO / 34 / behavior. The strongly polar NO stretching vibration / 35 / proved to be largely affected by the environment, as / 36 / suggested by resonant (interanionic) ([10] and references / 37 / therein) and non-resonant (lattice) effects experienced / 38 / by this stretching mode in salts containing the / 39 / [Fe(CN) 5 NO] 2( (NP) anion. [9,11] The MNO group, / 40 / in this and other pentaligandnitrosyl anions, [12 Á/17] / 41 / seems to be the site of the generation of two very long-/ 42 / lived metastable states (MSI and MSII) by irradiation at / 43 / low temperature with light in the 300 Á/550 nm wave-/ 44 / length range. A geometrical interpretation has been / 45 / proposed for MSI and MSII with a nearly linear / 46 / structure (FeON) for MSI and T-shaped Fe N O for MSII / 47 / [18] as results of very refined single crystal X-ray / 48 / diffraction studies. Recently, additional information / 49 / was obtained in favor of the linear, isonitrosyl structure / 50 / of MSI from the IR studies of 15 NO, N 18 O and 54 Fe-/ 51 / substituted excited sodium nitroprusside dihydrate / 52 / (SNP) and a DFT vibrational calculation [19].
Background 1,3-b-glucanases (group 2 of pathogenesis-related proteins) are enzymes widely distrib... more Background 1,3-b-glucanases (group 2 of pathogenesis-related proteins) are enzymes widely distributed among higher plants and have been recently proven to be significant allergens. Objective The aim of this work was to study the potential implication of 1,3-b-glucanases in crossreactivities among latex, pollen and vegetable foods. Methods The cDNA encoding the N-terminal domain (NtD) of Ole e 9, a major allergenic 1,3-bglucanase from olive pollen, was amplified by polymerase chain reaction and produced as a recombinant protein in Pichia pastoris (recombinant N-terminal domain, rNtD). Circular dichroism, ELISA, immunoblotting and immunoblotting inhibition experiments were carried out. Sera from olive pollen allergic patients and a rNtD-specific polyclonal antiserum were used. Results The NtD of Ole e 9 has been produced at high yield in the yeast P. pastoris and possesses 1,3-b-glucanase activity. The expressed polypeptide conserves IgE and IgG immunodominant epitopes of the whole Ole e 9. A rNtD-specific polyclonal antiserum and sera from olive pollen allergic patients allowed detection of IgG and IgE reactive peptidic epitopes common to 1,3-bglucanase Ole e 9 in extracts from ash and birch pollen, tomato, potato, bell-pepper, banana and latex. Conclusion rNtD and homologous glucanases are new molecules to be used in diagnostic protocols as they could help to identify allergic pollen patients who are at risk for developing allergic symptoms to fruits, vegetables and latex.
First measurements of dihadron correlations for charged particles are presented for central PbPb ... more First measurements of dihadron correlations for charged particles are presented for central PbPb collisions at a nucleon-nucleon center-of-mass energy of 2.76 TeV over a broad range in relative pseudorapidity (∆η) and the full range of relative azimuthal angle (∆φ). The data were collected with the CMS detector, at the LHC. A broadening of the away-side (∆φ ≈ π) azimuthal correlation is observed at all ∆η, as compared to the measurements in pp collisions. Furthermore, long-range dihadron correlations in ∆η are observed for particles with similar φ values. This phenomenon, also known as the "ridge", persists up to at least |∆η| = 4. For particles with transverse momenta
18 / Abstract / 19 / / 20 / The crystal structure of nitropentaamminecobalt(III) nitroprusside se... more 18 / Abstract / 19 / / 20 / The crystal structure of nitropentaamminecobalt(III) nitroprusside sesquihydrate, [Co(NH 3 ) 5 NO 2 ][Fe(CN) 5 NO] ×/1.5H 2 O has / 21 / been determined by Patterson and Fourier methods employing 3881 independent X-ray reflections with I /2s (I ) and refined by / 22 / full-matrix least-squares to R 1 0/0.032. The substance crystallizes in the monoclinic C 2/c (C 2h 6 ) space group No. 15, with a 0/ / 23 / 28.408(3), b 0/6.422(1), c 0/22.401(3) Å , b 0/123.55(1)8 and Z0/8. The metastable state I (MSI) of the nitroprusside anion and the / 24 / photochemical isomerization reaction of the nitropentaamminecobalt(III) cation to the nitrito isomer were produced by irradiation / 25 / with Ar ' laser light of 488 nm wavelength at 77 K. TGA and DTA of the non-excited substance were performed under nitrogen / 26 / atmosphere. # 2002 Published by Elsevier Science Ltd. / 27 / Keywords: PPPPentacyanonitrosylferrate(2(/) ion; Metastable excited states; X-ray diffraction; FIRRS; TGA; DTA / 28 / 1. Introduction / 29 / An extensive work on nitroprussides, which includes / 30 / alkaline, alkaline-earth, and transition metals salts has / 31 / been previously performed ([1 Á/10] and references / 32 / therein). These studies provided interesting results, / 33 / especially about the vibrational and photophysical NO / 34 / behavior. The strongly polar NO stretching vibration / 35 / proved to be largely affected by the environment, as / 36 / suggested by resonant (interanionic) ([10] and references / 37 / therein) and non-resonant (lattice) effects experienced / 38 / by this stretching mode in salts containing the / 39 / [Fe(CN) 5 NO] 2( (NP) anion. [9,11] The MNO group, / 40 / in this and other pentaligandnitrosyl anions, [12 Á/17] / 41 / seems to be the site of the generation of two very long-/ 42 / lived metastable states (MSI and MSII) by irradiation at / 43 / low temperature with light in the 300 Á/550 nm wave-/ 44 / length range. A geometrical interpretation has been / 45 / proposed for MSI and MSII with a nearly linear / 46 / structure (FeON) for MSI and T-shaped Fe N O for MSII / 47 / [18] as results of very refined single crystal X-ray / 48 / diffraction studies. Recently, additional information / 49 / was obtained in favor of the linear, isonitrosyl structure / 50 / of MSI from the IR studies of 15 NO, N 18 O and 54 Fe-/ 51 / substituted excited sodium nitroprusside dihydrate / 52 / (SNP) and a DFT vibrational calculation [19].
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