Papers by Liliana Daniela Vasile
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie
The aims of this study were to determine the diagnostic accuracy and reproducibility of the laser... more The aims of this study were to determine the diagnostic accuracy and reproducibility of the laser fluorescence device (LF), and the relationship between laser fluorescence readings taken at the entrance of the fissure, ICDAS visual examination caries detection system and the histological depth of the lesion. Two hundred and forty teeth (122 human third molars and 118 bicuspids) were selected from 62 patients enrolled in the study. Visual and LF examinations (Diagnodent, Kavo, Biberach, Germany) of the occlusal surfaces were performed in vivo. After tooth extraction, histological sections were evaluated by stereomicroscopy in vitro. Statistical analysis was performed using multiple statistical methods (SPSS ver. 17). Intra-examiner reproducibility for the LF measurements was excellent: intra-class-correlation coefficient (ICC) for LF was 0.957. Kappa values for each examiner's reproducibility were 0.74-0.82. The diagnostic performance of the LF device gave a good overall diagnost...
Electronomicroscopic evaluation of the microlesional aspects in the pulp dentinal complex after repeated whitening therapy
SPIE Proceedings, 2012
The aim of this study was to examine cellular and matriceal dynamics within pulp tissue of the te... more The aim of this study was to examine cellular and matriceal dynamics within pulp tissue of the teeth with repeated bleaching. Material and method - The study was made on 25 patients aged between 15 and 45, to whom bleaching method of the premolars with indication of extraction in orthodontic purposes was applied. None of the subjects smoked and throughout the investigation no antibiotics had been used. We initiated an intensive oral hygiene program, and we removed the supragingival and subgingival deposits. Oral hygiene and the gingival health were evaluated before every session of bleaching. During each visit the dentition was cleaned professionally and if needed the subjects were reinstucted in proper oral hygiene. After 3 and 5 successive bleachings of the teeth, we removed the dental pulps and we extracted the premolars. The pulpal biopsies were fixed in buffed formaldehyde 10% for 48 hours, then paraffinized, sectioned at 3-5 μ and stained with topographic, H&E and trichrome stained. For the electonomicroscopic study we used the Lehner technique to process the biopsies (n=3) after the reinclusion of the pieces from the paraffine blocks in Epon, postfixated in buffered glutaraldehyde, micro sectioned at 0,5 μ, contrastated with Pb citrate (stained) and examination in transmission electronic microscopy with Philips microscope. Results - At cellular and matriceal level we observed a marked collagen fibrillogenesis in the presence of active fibroblasts, with well developed cellular organites and fibroclastic aspects which suggest matriceal active repair. The microvascular network presents an activated endothelium with turgescent endothelial cells, with intracitoplasmatic resorbtion vacuols, well developed Golgi Complex. Conclusion - We interpreed the cell - matriceal lesions in the context of the acute inflammatory process in the first lesional phase and chronic scleroatrophic process after successive bleaching.
[Some aspects of pulmonary vascular remodelling in smokers and patients with mild COPD]
Pneumologia (Bucharest, Romania)
An important feature of the pathophysiology of COPD patients is represented by the remodelling of... more An important feature of the pathophysiology of COPD patients is represented by the remodelling of pulmonary vasculature. Within this process, a pathogenic element might be the endothelial dysfunction induced by the combined effect of several factors such as: cigarette smoke, inflammatory mediators, hypoxia, mechanical stress, whose contribution differs according to the stage of the disease. The aims of our study were to characterize the structural pulmonary vascular abnormalities present in smokers and patients with mild COPD and to assess their pathophysiological importance. Serial tissue sections from fragments of pulmonary exeresis have been subjected to both morphometric analysis and histochemical examination of muscular pulmonary arteries. Our results have shown a process of arteriolar muscularization and intimal thickening of small muscular arteries in both smokers (intimal index 23.11% +/- 3.04%) and patients with mild COPD (25.62% +/- 3.06%) as compared to controls (17.86% +...
Stereomicroscopic study of the human tooth caries: clinical and morphological correlations
SPIE Proceedings, 2012
Objectives: Stereomicroscopy allows a three-dimensional study of the images and of laterality at ... more Objectives: Stereomicroscopy allows a three-dimensional study of the images and of laterality at superior quality in comparison with other methods. Those advantages are given by the large examination fields and the wide work distances. The adding of the clinical and morphological data at the results gathered with stereomicroscopy and the stereo micrometry is useful in order to appreciate the deepness and the widening of the carious process, and the necessity to reconsider the therapeutically strategy. Materials and methods: During 2009-2011 the study material was represented by 10 surgically removed impacted third molars, and by 20 premolars extracted for orthodontic purposes, with closed and macroscopically apparently integer surfaces. 13 premolars with different degrees of carious affectation and periodontal lesions, which were surgically extracted without trauma, were also selected. The in situ measurements at the occlusal site were realized through the utilization of a fluorescent laser device - DIAGNOdent. The basic principles in stereomicroscopy stood at the base of the obliquely and circularly coaxial illumination techniques, one with optical alignment adjustment of the optical microscope and mechanical adjustment for the optimal illumination and micrometry. The Olympus Microscope SZ ×7 and an Olympus camera with 2,5 × digital zoom and a 3× optical zoom has been used to study the samples in stereomicroscopy and through polarized light it. Results: The DiagnoDent measured the following data: out of 43 apparently healthy teeth, 18 presented values between 2 and 13 (D1), 13 showed values between 14 and 24 (D2), 12 measured values over 24 (D3). After the histological examination in stereomicroscopy and in the polarized light: 25 teeth were healthy, 10 presented caries extended in dental enamel and 8 presented dentinal caries. Stereomicroscopy has allowed the morphological study, the color absorption, the appreciation of the lesions' deepness and substance loss that is very useful in grading the progression of the carious lesion. Conclusions: The stereomicroscopic study correlated with clinical and morphological data allowed to appreciate the extent of tissue involved in the carious process, but also the understanding of the enamel, dentine and cement matrix demineralization process, in proximity with the morpho-embryological markings of the human tooth structure.
SPIE Proceedings, 2011
Scop şi obiective. Leziunile cervicale necarioase (LCN) sunt pierderi de substanţă dură dentară d... more Scop şi obiective. Leziunile cervicale necarioase (LCN) sunt pierderi de substanţă dură dentară de la nivelul joncţiunii smalţ -cement. Ele pot fi provocate de către abraziune, eroziune şi/sau suprasolicitări ocluzale para-axiale. Dinţi frontali extraşi au fost studiaţi prin tomografie optic coerentă en face (eFTOC) pentru a evalua ponderea factorilor ocluzali în etiologia LCN. Material şi metodă. Au fost analizaţi 35 de dinţi frontali extraşi de la pacienţi cu bruxism activ şi interferenţe ocluzale excentrice, prezentând LCN pe suprafeţele vestibulare. Ceilalţi 5 dinţi frontali studiaţi, cu o morfologie coronară normală, nu au fost supuşi pre-extracţional la suprasolicitări ocluzale. Probele dentare au fost examinate cu ajutorul unui dispozitiv TOC en face ce lucrează la 1300 nm (B-scan şi C-scan). Rezultate. Dinţii frontali cu o morfologie coronară normală au prezentat pe imaginile de eFTOC o microstructură omogenă a ţesuturilor dure cervicale. La dinţii frontali suprasolicitaţi ocluzal, investigaţia eFOCT a evidenţiat fisuri cu un tipar caracteristic în zona LCN. Concluzii. eFTOC este o tehnică imagistică care identifică eficient efectele microstructurale pe care suprasolicitarea ocluzală le produce asupra ţesuturilor dure din regiunea cervicală a dinţilor frontali. Cuvinte cheie: leziuni cervicale necarioase, abfracţie, suprasolicitare ocluzală, bruxism, tomografie optic coerentă en face.
Preliminary optical coherence tomography investigation of the temporo-mandibular joint disc
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XIV, 2010
Aim and objectives. The morphology and position of the temporo-mandibular disc are key issues in ... more Aim and objectives. The morphology and position of the temporo-mandibular disc are key issues in the diagnosis and treatment of arthrogenous temporo-mandibular disorders. Magnetic resonance imaging and arthroscopy are used today to identify: flattening of the ...
<title>Stereomicroscopic evaluation of the joint cartilage and bone tissue in osteoporosis</title>
Biophotonics: Photonic Solutions for Better Health Care III, 2012
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Papers by Liliana Daniela Vasile