Objetivo: Avaliar in vitro a microinfiltracao em restauracoes confeccionadas em molares deciduos ... more Objetivo: Avaliar in vitro a microinfiltracao em restauracoes confeccionadas em molares deciduos com diferentes tecnicas adesivas. Metodo: Foram confeccionados preparos cavitarios nas faces vestibular e lingual, divididos aleatoriamente em quatro grupos: G1 (n=10) sistema adesivo autocondicionante (Adper Prompt L-Pop); G2 (n=10) sistema adesivo convencional (Adper Single Bond 2); G3 (n=10) condicionamento com acido fosforico a 37% e sistema adesivo autocondicionante (Self-etch Bond); G4 (n=10) sistema adesivo autocondicionante (Self-etch Bond). Os grupos 1 e 2 foram restaurados com resina composta Concept Advanced (Vigodent) e os grupos 3 e 4 com a resina composta Z100 (3M). Em seguida, procederam-se a ciclagem termica (±5o e ±55oC, 125 ciclos, 10 segundos em cada banho), impermeabilizacao (cola de presa rapida e esmalte de unha), imersao em Fucsina basica a 0,5% e seccionamento no sentido vestibulo-lingual. A avaliacao foi realizada em microscopio estereoscopico, com aumento de 25 ...
This study was conducted to investigate whether polymorphisms of genes involved in immune checkpo... more This study was conducted to investigate whether polymorphisms of genes involved in immune checkpoints can predict the clinical outcomes of patients with advanced stage non-small cell lung cancer (NSCLC) after 1st line paclitaxel-cisplatin chemotherapy. A total of 379 NSCLC patients were enrolled. Twelve single nucleotide polymorphisms (SNPs) of PD-1, PD-L1, and CTLA-4 genes were selected and genotyped. The associations of SNPs with chemotherapy response and overall survival (OS) were analyzed. Among the 12 SNPs investigated, PD-L1 rs2297136T > C and rs4143815C > G were significantly associated with clinical outcomes after chemotherapy. The rs2297136T > C was significantly associated with both better chemotherapy response and better OS, and the rs4143815C > G had a significantly better response to chemotherapy. Consistent with the individual genotype analyses, rs2297136C-rs4143815G haplotype (ht4) carrying variant alleles at both loci was significantly associated with bet...
Although non-small cell lung cancer (NSCLC) remains a deadly disease, new predictive biomarkers h... more Although non-small cell lung cancer (NSCLC) remains a deadly disease, new predictive biomarkers have emerged to assist in managing the disease, of which one of the most promising is the programmed death-ligand 1 (PD-L1). Each, PD-L1 variant seem to modulate the function of immune checkpoints differently and affect response to adjuvant treatment and outcome in NSCLC patients. We thus investigated the influence of these PD-L1 genetic variations in genetically admixed NSCLC tissue samples, and correlated these values with clinicopathological characteristics, including prognosis. Materials and Methods: We evaluated PD-L1 non-coding genetic variants and protein expression in lung adenocarcinomas (ADC), squamous cell carcinomas (SqCC), and large cell carcinomas (LCC) in silico. Microarray paraffin blocks from 70 samples of ADC (N=33), SqCC (N=24), and LCC (N=13) were used to create PD-L1 multiplex immunofluorescence assays with a Cell Signaling E1L3N clone. Fifteen polymorphisms of the PD-L1 gene were investigated by targeted sequencing and evaluated in silico using dedicated tools. Results: Although PD-L1 polymorphisms seemed not to interfere with protein expression, PD-L1 expression varied among different histological subtypes, as did clinical outcomes, with the rs4742098A>G, rs4143815G>C, and rs7041009G>A variants being associated with relapse (P=0.01; P=0.05; P=0.02, respectively). The rs7041009 GG genotype showed a significant correlation with younger and alive patients compared to carriers of the A allele (P=0.02 and P<0.01, respectively). The Cox regression model showed that the rs7041009 GG genotype may influence OS (P<0.01) as a co-dependent factor associated with radiotherapy and recurrence in NSCLC patients. Furthermore, the Kaplan-Meier survival curves showed that rs7041009 and rs4742098 might impact PPS in relapsed patients. In silico approaches identified the variants as benign. Conclusion: PD-L1 non-coding variants play an important role in modulating immune checkpoint function and may be explored as immunotherapy biomarkers. We highlight the rs7041009 variant, which impacts OS and PPS in NSCLC patients.
Objective Between 2010 and 2017, it was registered one of the most intense droughts in the Brazil... more Objective Between 2010 and 2017, it was registered one of the most intense droughts in the Brazilian Northeastern Semiarid region, when the rainfall was below the historical level for the region. This context was related to water scarcity for human consumption and productive activities in this territory, where the semiarid backlands of the state of Pernambuco are located. The objective of this study was to assess the condition of food security and its associated factors in areas affected by drought in the semiarid zone of Pernambuco, Brazil. Methods It was a cross-sectional study on a populational base. Data were collected by socioeconomic and demographic surveys, and the Brazilian Food Insecurity Scale, which were applied to 252 households, from September to October 2015. The data was analyzed by socioeconomic and demographic characterization of the households, and the identification of the food insecurity prevalence and its associated factors, by Poisson regression. Results The pr...
Thymulin has been shown to present anti-inflammatory and anti-fibrotic properties in experimental... more Thymulin has been shown to present anti-inflammatory and anti-fibrotic properties in experimental lung diseases. We hypothesized that a biologically active thymulin analog gene, methionine serum thymus factor, delivered by highly compacted DNA nanoparticles composed of single molecule of plasmid DNA compacted with block copolymers of poly-L-lysine and polyethylene glycol (CK 30 PEG) that have been found safe in a human phase I clinical trial, may prevent lung inflammation and remodeling in a mouse model of allergic asthma. Thymulin plasmids were detected in the lungs of ovalbumin-challenged asthmatic mice up to 27 days after
Introducao : Uma comunidade de pratica consiste num grupo de pessoas que se unem em torno de um m... more Introducao : Uma comunidade de pratica consiste num grupo de pessoas que se unem em torno de um mesmo topico ou interesse, trabalhando em conjunto para melhorar o que fazem atraves de interacoes regulares. A Comunidade Formativa do Estoril reune o grupo de orientadores e internos de medicina geral e familiar e saude publica da zona do Estoril, com o objetivo de alargar o seu leque de experiencias e vivencias, espelhando os principios de uma comunidade de pratica. Este artigo relata a experiencia desta Comunidade e tem como principal objetivo dar a conhecer as suas praticas, incentivar a partilha de experiencias entre grupos e inspirar a formacao de novas comunidades. Descricao : A Comunidade Formativa do Estoril nasceu no ano de 2015, composta pelos internos e orientadores de formacao especifica em medicina geral e familiar e de saude publica de quatro das unidades funcionais do edificio de Sao Joao do Estoril do Agrupamento de Centros de Saude (ACeS) de Cascais. Desde a criacao da ...
This is a preliminary version of the scoping review protocol, published as a preprint to elicit f... more This is a preliminary version of the scoping review protocol, published as a preprint to elicit feedback from the scientific community. Constructive feedback is highly welcome and can be sent via e-mail, Twitter, or using hypothes.is . The protocol will be subsequently revised in light of the feedback and a final version will be uploaded. Correspondence concerning this protocol should be addressed to Vasco Brazão. Contact: [email protected] , alternatively @vascobrazao .
Conclusion: HC-based MPS changed therapeutic strategy in close to half of the patients with lung ... more Conclusion: HC-based MPS changed therapeutic strategy in close to half of the patients with lung cancer, and was associated with high overall response rate, which may translate into a survival benefit.
RESUMO Objetivo: Determinar a prevalência do excesso de peso e fatores associados em mulheres em ... more RESUMO Objetivo: Determinar a prevalência do excesso de peso e fatores associados em mulheres em idade reprodutiva no município de Vitória de Santo Antão, Pernambuco. Métodos: Estudo transversal com 322 mulheres, conduzido entre os meses de janeiro e abril de 2013 através de busca ativa nos domicílios. O estado nutricional foi classificado pelo índice de massa corporal para as maiores de 18 anos e pelo índice de massa corporal/idade para as adolescentes. A variável dependente foi o excesso de peso (sobrepeso/obesidade) e os fatores associados (variáveis independentes) foram: as condições socioeconômicas e demográficas (idade, escolaridade, alfabetização, trabalho remunerado, classe econômica, condição de segurança alimentar e nutricional, número de pessoas no domicílio, condições de saneamento básico, piso, teto, número de cômodos e de dormitórios), comportamental (prática de atividade física) e história reprodutiva (menarca, número de gestações e método contraceptivo). A regressão ...
C95. FUTURE BIO-THERAPIES IN CRITICAL CARE MEDICINE: OF MICE AND MEN, 2010
Page 1. / Mini Symposium / Tuesday, May 18/1:30 C95 FUTURE BIO-THERAPIES IN CRITICAL CARE MEDICIN... more Page 1. / Mini Symposium / Tuesday, May 18/1:30 C95 FUTURE BIO-THERAPIES IN CRITICAL CARE MEDICINE: OF MICE AND MEN PM-4:00 PM / Room 271-273 (Second Level), Morial Convention Center Exercise Modulates ...
Critical leg ischemia is associated with a high risk of amputation when revascularization is not ... more Critical leg ischemia is associated with a high risk of amputation when revascularization is not possible. Cell therapy based on bone marrow-derived mononuclear cells or with peripheral mononuclear cells, collected after stimulation with G-CSF has been used in an attempt to stimulate angiogenesis. Although several studies have raised the hope that such cell therapy may be effective in critical leg ischemia, no direct demonstration of angiogenesis induced by bone marrow-derived mononuclear cell/peripheral mononuclear cell injection has been reported in man. The aim of this study was to identify and to evaluate the extent of the angiogenic process associated with cell therapy in critical leg ischemia in man. To address this question, this pathological study was conducted in patients enrolled in the OPTIPEC clinical trial (Optimization of Progenitor Endothelial Cells in the Treatment of Critical leg ischemia), an interventional cell therapy study in critical leg ischemia. Amputation specimens from these patients were submitted to a standardized dissection protocol. In three patients, an active angiogenesis was observed in the distal part of the ischemic limb but not in the gastrocnemius muscle, the site of bone marrow cell injection. All the newly formed vessels were positive for endothelial cell markers (CD31, CD34, von Willebrand factor) and negative for markers of lymphatic vessels (podoplanin). Immunohistochemical staining for Ki-67 and c-kit showed extensive endothelial cell proliferation within the new vessels. Bone marrow-derived mononuclear cell therapy in patients with critical leg ischemia induces an active, substained angiogenesis in the ischemic and distal parts of the treated limb, although this may not prevent amputation in some patients with very severe ischemia.
We hypothesized that bone marrow-derived mononuclear cell (BMDMC) therapy protects the lung and c... more We hypothesized that bone marrow-derived mononuclear cell (BMDMC) therapy protects the lung and consequently the heart in experimental elastase-induced emphysema. Twenty-four female C57BL/6 mice were intratracheally instilled with saline (C group) or porcine pancreatic elastase (E group) once a week during 4 weeks. C and E groups were randomized into subgroups receiving saline (SAL) or male BMDMCs (2 × 10 6 , CELL) intravenously 3 h after the first saline or elastase instillation. Compared to E-SAL group, E-CELL mice showed, at 5 weeks: lower mean linear intercept, neutrophil infiltration, elastolysis, collagen fiber deposition in alveolar septa and pulmonary vessel wall, lung cell apoptosis, right ventricle wall thickness and area, higher endothelial growth factor and insulin-like growth factor mRNA expressions in lung tissue, and reduced platelet-derived growth factor, transforming growth factor-, and caspase-3 expressions. In conclusion, BMDMC therapy was effective at modulating the inflammatory and remodeling processes in the present model of elastase-induced emphysema.
Objetivo: Avaliar in vitro a microinfiltracao em restauracoes confeccionadas em molares deciduos ... more Objetivo: Avaliar in vitro a microinfiltracao em restauracoes confeccionadas em molares deciduos com diferentes tecnicas adesivas. Metodo: Foram confeccionados preparos cavitarios nas faces vestibular e lingual, divididos aleatoriamente em quatro grupos: G1 (n=10) sistema adesivo autocondicionante (Adper Prompt L-Pop); G2 (n=10) sistema adesivo convencional (Adper Single Bond 2); G3 (n=10) condicionamento com acido fosforico a 37% e sistema adesivo autocondicionante (Self-etch Bond); G4 (n=10) sistema adesivo autocondicionante (Self-etch Bond). Os grupos 1 e 2 foram restaurados com resina composta Concept Advanced (Vigodent) e os grupos 3 e 4 com a resina composta Z100 (3M). Em seguida, procederam-se a ciclagem termica (±5o e ±55oC, 125 ciclos, 10 segundos em cada banho), impermeabilizacao (cola de presa rapida e esmalte de unha), imersao em Fucsina basica a 0,5% e seccionamento no sentido vestibulo-lingual. A avaliacao foi realizada em microscopio estereoscopico, com aumento de 25 ...
This study was conducted to investigate whether polymorphisms of genes involved in immune checkpo... more This study was conducted to investigate whether polymorphisms of genes involved in immune checkpoints can predict the clinical outcomes of patients with advanced stage non-small cell lung cancer (NSCLC) after 1st line paclitaxel-cisplatin chemotherapy. A total of 379 NSCLC patients were enrolled. Twelve single nucleotide polymorphisms (SNPs) of PD-1, PD-L1, and CTLA-4 genes were selected and genotyped. The associations of SNPs with chemotherapy response and overall survival (OS) were analyzed. Among the 12 SNPs investigated, PD-L1 rs2297136T > C and rs4143815C > G were significantly associated with clinical outcomes after chemotherapy. The rs2297136T > C was significantly associated with both better chemotherapy response and better OS, and the rs4143815C > G had a significantly better response to chemotherapy. Consistent with the individual genotype analyses, rs2297136C-rs4143815G haplotype (ht4) carrying variant alleles at both loci was significantly associated with bet...
Although non-small cell lung cancer (NSCLC) remains a deadly disease, new predictive biomarkers h... more Although non-small cell lung cancer (NSCLC) remains a deadly disease, new predictive biomarkers have emerged to assist in managing the disease, of which one of the most promising is the programmed death-ligand 1 (PD-L1). Each, PD-L1 variant seem to modulate the function of immune checkpoints differently and affect response to adjuvant treatment and outcome in NSCLC patients. We thus investigated the influence of these PD-L1 genetic variations in genetically admixed NSCLC tissue samples, and correlated these values with clinicopathological characteristics, including prognosis. Materials and Methods: We evaluated PD-L1 non-coding genetic variants and protein expression in lung adenocarcinomas (ADC), squamous cell carcinomas (SqCC), and large cell carcinomas (LCC) in silico. Microarray paraffin blocks from 70 samples of ADC (N=33), SqCC (N=24), and LCC (N=13) were used to create PD-L1 multiplex immunofluorescence assays with a Cell Signaling E1L3N clone. Fifteen polymorphisms of the PD-L1 gene were investigated by targeted sequencing and evaluated in silico using dedicated tools. Results: Although PD-L1 polymorphisms seemed not to interfere with protein expression, PD-L1 expression varied among different histological subtypes, as did clinical outcomes, with the rs4742098A>G, rs4143815G>C, and rs7041009G>A variants being associated with relapse (P=0.01; P=0.05; P=0.02, respectively). The rs7041009 GG genotype showed a significant correlation with younger and alive patients compared to carriers of the A allele (P=0.02 and P<0.01, respectively). The Cox regression model showed that the rs7041009 GG genotype may influence OS (P<0.01) as a co-dependent factor associated with radiotherapy and recurrence in NSCLC patients. Furthermore, the Kaplan-Meier survival curves showed that rs7041009 and rs4742098 might impact PPS in relapsed patients. In silico approaches identified the variants as benign. Conclusion: PD-L1 non-coding variants play an important role in modulating immune checkpoint function and may be explored as immunotherapy biomarkers. We highlight the rs7041009 variant, which impacts OS and PPS in NSCLC patients.
Objective Between 2010 and 2017, it was registered one of the most intense droughts in the Brazil... more Objective Between 2010 and 2017, it was registered one of the most intense droughts in the Brazilian Northeastern Semiarid region, when the rainfall was below the historical level for the region. This context was related to water scarcity for human consumption and productive activities in this territory, where the semiarid backlands of the state of Pernambuco are located. The objective of this study was to assess the condition of food security and its associated factors in areas affected by drought in the semiarid zone of Pernambuco, Brazil. Methods It was a cross-sectional study on a populational base. Data were collected by socioeconomic and demographic surveys, and the Brazilian Food Insecurity Scale, which were applied to 252 households, from September to October 2015. The data was analyzed by socioeconomic and demographic characterization of the households, and the identification of the food insecurity prevalence and its associated factors, by Poisson regression. Results The pr...
Thymulin has been shown to present anti-inflammatory and anti-fibrotic properties in experimental... more Thymulin has been shown to present anti-inflammatory and anti-fibrotic properties in experimental lung diseases. We hypothesized that a biologically active thymulin analog gene, methionine serum thymus factor, delivered by highly compacted DNA nanoparticles composed of single molecule of plasmid DNA compacted with block copolymers of poly-L-lysine and polyethylene glycol (CK 30 PEG) that have been found safe in a human phase I clinical trial, may prevent lung inflammation and remodeling in a mouse model of allergic asthma. Thymulin plasmids were detected in the lungs of ovalbumin-challenged asthmatic mice up to 27 days after
Introducao : Uma comunidade de pratica consiste num grupo de pessoas que se unem em torno de um m... more Introducao : Uma comunidade de pratica consiste num grupo de pessoas que se unem em torno de um mesmo topico ou interesse, trabalhando em conjunto para melhorar o que fazem atraves de interacoes regulares. A Comunidade Formativa do Estoril reune o grupo de orientadores e internos de medicina geral e familiar e saude publica da zona do Estoril, com o objetivo de alargar o seu leque de experiencias e vivencias, espelhando os principios de uma comunidade de pratica. Este artigo relata a experiencia desta Comunidade e tem como principal objetivo dar a conhecer as suas praticas, incentivar a partilha de experiencias entre grupos e inspirar a formacao de novas comunidades. Descricao : A Comunidade Formativa do Estoril nasceu no ano de 2015, composta pelos internos e orientadores de formacao especifica em medicina geral e familiar e de saude publica de quatro das unidades funcionais do edificio de Sao Joao do Estoril do Agrupamento de Centros de Saude (ACeS) de Cascais. Desde a criacao da ...
This is a preliminary version of the scoping review protocol, published as a preprint to elicit f... more This is a preliminary version of the scoping review protocol, published as a preprint to elicit feedback from the scientific community. Constructive feedback is highly welcome and can be sent via e-mail, Twitter, or using hypothes.is . The protocol will be subsequently revised in light of the feedback and a final version will be uploaded. Correspondence concerning this protocol should be addressed to Vasco Brazão. Contact: [email protected] , alternatively @vascobrazao .
Conclusion: HC-based MPS changed therapeutic strategy in close to half of the patients with lung ... more Conclusion: HC-based MPS changed therapeutic strategy in close to half of the patients with lung cancer, and was associated with high overall response rate, which may translate into a survival benefit.
RESUMO Objetivo: Determinar a prevalência do excesso de peso e fatores associados em mulheres em ... more RESUMO Objetivo: Determinar a prevalência do excesso de peso e fatores associados em mulheres em idade reprodutiva no município de Vitória de Santo Antão, Pernambuco. Métodos: Estudo transversal com 322 mulheres, conduzido entre os meses de janeiro e abril de 2013 através de busca ativa nos domicílios. O estado nutricional foi classificado pelo índice de massa corporal para as maiores de 18 anos e pelo índice de massa corporal/idade para as adolescentes. A variável dependente foi o excesso de peso (sobrepeso/obesidade) e os fatores associados (variáveis independentes) foram: as condições socioeconômicas e demográficas (idade, escolaridade, alfabetização, trabalho remunerado, classe econômica, condição de segurança alimentar e nutricional, número de pessoas no domicílio, condições de saneamento básico, piso, teto, número de cômodos e de dormitórios), comportamental (prática de atividade física) e história reprodutiva (menarca, número de gestações e método contraceptivo). A regressão ...
C95. FUTURE BIO-THERAPIES IN CRITICAL CARE MEDICINE: OF MICE AND MEN, 2010
Page 1. / Mini Symposium / Tuesday, May 18/1:30 C95 FUTURE BIO-THERAPIES IN CRITICAL CARE MEDICIN... more Page 1. / Mini Symposium / Tuesday, May 18/1:30 C95 FUTURE BIO-THERAPIES IN CRITICAL CARE MEDICINE: OF MICE AND MEN PM-4:00 PM / Room 271-273 (Second Level), Morial Convention Center Exercise Modulates ...
Critical leg ischemia is associated with a high risk of amputation when revascularization is not ... more Critical leg ischemia is associated with a high risk of amputation when revascularization is not possible. Cell therapy based on bone marrow-derived mononuclear cells or with peripheral mononuclear cells, collected after stimulation with G-CSF has been used in an attempt to stimulate angiogenesis. Although several studies have raised the hope that such cell therapy may be effective in critical leg ischemia, no direct demonstration of angiogenesis induced by bone marrow-derived mononuclear cell/peripheral mononuclear cell injection has been reported in man. The aim of this study was to identify and to evaluate the extent of the angiogenic process associated with cell therapy in critical leg ischemia in man. To address this question, this pathological study was conducted in patients enrolled in the OPTIPEC clinical trial (Optimization of Progenitor Endothelial Cells in the Treatment of Critical leg ischemia), an interventional cell therapy study in critical leg ischemia. Amputation specimens from these patients were submitted to a standardized dissection protocol. In three patients, an active angiogenesis was observed in the distal part of the ischemic limb but not in the gastrocnemius muscle, the site of bone marrow cell injection. All the newly formed vessels were positive for endothelial cell markers (CD31, CD34, von Willebrand factor) and negative for markers of lymphatic vessels (podoplanin). Immunohistochemical staining for Ki-67 and c-kit showed extensive endothelial cell proliferation within the new vessels. Bone marrow-derived mononuclear cell therapy in patients with critical leg ischemia induces an active, substained angiogenesis in the ischemic and distal parts of the treated limb, although this may not prevent amputation in some patients with very severe ischemia.
We hypothesized that bone marrow-derived mononuclear cell (BMDMC) therapy protects the lung and c... more We hypothesized that bone marrow-derived mononuclear cell (BMDMC) therapy protects the lung and consequently the heart in experimental elastase-induced emphysema. Twenty-four female C57BL/6 mice were intratracheally instilled with saline (C group) or porcine pancreatic elastase (E group) once a week during 4 weeks. C and E groups were randomized into subgroups receiving saline (SAL) or male BMDMCs (2 × 10 6 , CELL) intravenously 3 h after the first saline or elastase instillation. Compared to E-SAL group, E-CELL mice showed, at 5 weeks: lower mean linear intercept, neutrophil infiltration, elastolysis, collagen fiber deposition in alveolar septa and pulmonary vessel wall, lung cell apoptosis, right ventricle wall thickness and area, higher endothelial growth factor and insulin-like growth factor mRNA expressions in lung tissue, and reduced platelet-derived growth factor, transforming growth factor-, and caspase-3 expressions. In conclusion, BMDMC therapy was effective at modulating the inflammatory and remodeling processes in the present model of elastase-induced emphysema.
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