Papers by Virgínia Pedrosa
Dietary addition of recombinant Bacillus subtilis expressing a fungal phytase increases phosphorus fixation in muscle of Pacific white shrimp Litopenaeus vannamei
Aquaculture International
Fishes
The effects of temperature manipulation, addition of sperm solution, and exposure to alkalized pH... more The effects of temperature manipulation, addition of sperm solution, and exposure to alkalized pH and/or hydrogen peroxide (H2O2) as possible spawning inducers in laboratory-conditioned and unconditioned adults of the yellow clam (Amarilladesma mactroides) were evaluated. In three trials, clams were laboratory-conditioned for 14 days and exposed to thermal shocks (from 20 °C to 23–29 °C), while clams from three additional trials were not conditioned but acclimatized for 45 min before spawning induction. Although conditioning advanced gonad maturity and increased the condition factor, none of the thermal treatments triggered spawning in these first trials. Histological analysis indicated that the gonads of conditioned clams were not mature. Alternatively, unfertilized and fertilized eggs, and larvae were observed after unconditioned clams were induced to spawn. The gonads of unconditioned clams were in an advanced stage of maturity. Exposure to temperature shocks, alone or in combina...
Brazilian Journal of Biology
The yellow clam is a sand-burrowing bivalve that inhabits the dissipative beaches from southern B... more The yellow clam is a sand-burrowing bivalve that inhabits the dissipative beaches from southern Brazil to the north coast of Argentina. In the last decades, populations of this species have been impacted by mass mortality events, overfishing and other anthropogenic activities. The production of juveniles in captivity would allow feasibility studies to be carried out to restore the natural stock as well as the production in aquaculture systems. Given the scarcity of studies on the maintenance of this species in captivity, a culture system and a management protocol were developed and tested. Wild-caught clams (total length ≥50 mm) were used in a series of 14 day-long trials. Survival was higher in clams that were allowed to bury into the sand. A permanent ink marker covered with a thin layer of a quick-hardening adhesive proved to be a reliable method to tag clams. The maintenance of yellow clams in this system resulted in high survival and growth, increases in the condition factor an...
Aquaculture, 2021
The aim of this article is to understand the relationship between two of the Sustainable Developm... more The aim of this article is to understand the relationship between two of the Sustainable Development Goals (UN Agenda 2030)-Good health and well-being (SDG 3) and Clean water and sanitation (SDG 6)-and the statistics of the COVID-19 pandemic (number of cases and deaths) in Brazilian cities. To analyze this relationship, we used secondary data from public organizations on the SDG panorama by city and conducted a moderated regression analysis. The sample was composed of 649 cities with a population exceeding 50 thousand inhabitants. The results show that the higher were the indicators used to measure SDGs, the lower was the number of cases and deaths from the disease. We have also proved that cities' population density and their distance from the pandemic epicenter moderate this relationship, since a higher level of these moderation variables increases the impact of a lower level of SDGs 3 and 6 coverage in society on the number of cases and deaths from COVID-19. Thus, the efficient and effective investment to reach SDGs 3 and 6 is directly associated with cities' ability to successfully deal with infectious diseases and the resulting number of deaths. As for its contribution, this research innovates by establishing a model for analyzing the impact of compliance with SDGs on cities' performance in their fight against COVID-19, which may also suit other nations.
Inovações na Nutrição Animal: desafios da produção de qualidade, 2021
AquaTechnica: Revista Iberoamericana de Acuicultura., 2021
En este trabajo describimos dos neurofibromas en Betta splendens estudiados con microscopía óptic... more En este trabajo describimos dos neurofibromas en Betta splendens estudiados con microscopía óptica, inmunohistoquímica y microscopía electrónica. El espécimen estudiado, que pertenecía a un criador privado, presentaba marcada distensión abdominal, fue trasladado para diagnóstico al Laboratorio de Inmunología y Patología de Organismos Acuáticos de la Universidade Federal do Rio Grande – FURG. Fue diagnosticado como neurofibroma, positivo para el anticuerpo contra proteína S-100. Con microscopia electrónica, mostraba signos de degeneración en algunas células y contenía mastocitos distribuidos regularmente en la masa neoplásica. Con inmunohistoquímica confirmamos la histogénesis de la neoplasia, en la que existen dos neurofibromas, y con microscopía electrónica confirmamos que las células granulares que se encuentran entre el tejido neoplásico son MC.
Brazilian Journal of Veterinary Pathology, 2020
The present study reports a case of leucism in South American Lungfish Lepidosiren paradoxa captu... more The present study reports a case of leucism in South American Lungfish Lepidosiren paradoxa captured in Corrientes, Argentina. It was observed a change in cutaneous pigmentation, and it was concluded that it was leucism and not albinism, since there was a decrease of tegumentary melanin pigment and normal pigmentation in the retina.
Mycobacteria are widespread in the natural environment, both in terrestrial and aquatic environme... more Mycobacteria are widespread in the natural environment, both in terrestrial and aquatic environments, and some species can be zoonotic. In aquatic organisms, especially in fish and crustaceans, they are diagnosed with certain frequency. Taking into account that mycobacteria affecting fish, as well as the species causing leprosy, have less mycolic acid, the authors used the Fite-Faraco (FF) technique in this work and compared it with the classic Ziehl Neelsen (ZN) staining. In the Laboratory of Immunology and Pathology of Aquatic Organisms (LIPOA), the authors chose from their tissue archive tissue material belonging to 10 specimens of fish of different species, belonging to LIPOA, which were reported cases over a period of 15 years (from 2002 to 2017). The Original Research Article Romano et al.; AJRAVS, 6(1): 27-34, 2020; Article no.AJRAVS.59001 28 specimens had in common the presence of lesions classified as granulomatous inflammation, but with the ZN technique it was not possible...
Frontiers in Physiology, 2021
Graphical GH overexpression increases the intestinal mass and absorptive surface area both throug... more Graphical GH overexpression increases the intestinal mass and absorptive surface area both through the direct effects of GH transgenics (up-regulating the expression of its cognate receptor and insulin-like growth factor, igf1a) and through indirect effects through an increase in the amount of food consumed. In addition, hyperphagia provides a greater amount of nutrients in the intestinal lumen and stimulates the synthesis of di- and tri-peptide transporters, which are the main route of absorption of dietary products from protein degradation.
Mast cells and eosinophilic granule cells in Oncorhynchus mykiss: Are they similar or different?
Fish and Shellfish Immunology Reports, 2021
Asian Journal of Research in Zoology, 2021
The aim of this study was to determine the relationship between EGC degranulation in fish injecte... more The aim of this study was to determine the relationship between EGC degranulation in fish injected with formalin-killed Escherichia coli and the effect of dexamethasone, diphenhydramine supplied separately and before formalin-killed E. coli. We performed a quantitative analysis of the number of cell granules and demonstrated that: compared to the EGCs of animals, the injection of dead E. coli with formalin generated degranulation of the EGC, while the administration of dexamethasone alone did not show significant differences with control group animals. The administration of diphenhydramine alone did not show significant differences neither with the animals of the dexamethasone treated group nor with those of the control group. When dexamethasone was administered one hour before the E. coli injection, degranulation was apparently inhibited and the number of granules did not show significant differences either with the animals in the control group or with those treated with dexamethas...
Asian Journal of Research in Zoology, 2021
A capsulated nodular neoplasm measuring 9 cm in diameter, located between the swim bladder and th... more A capsulated nodular neoplasm measuring 9 cm in diameter, located between the swim bladder and the kidney sample of Cyprinuscarpio, was in laboratory. The neoplasm had a solid consistency in one sector and very adherent to the capsule. Histopathological examination of the neoplasm revealed a monotonous proliferation of spindle cells, and pleomorphic, sometimes in a vortex pattern and interspersed with collagen fibers. Cells had numerous well-oriented myofibrils giving them a deep red. The cytoplasm is eosinophilic and the nuclei are hyperchromatic located in the center with blunt or "cigar-shaped" ends. In the immunohistochemical examination, the tumor cells were positive for desmin, smooth muscle actin and K-47, the latter antibody showing significant cell proliferation. Due to histopathological and immunohistochemical findings, diagnosis was made with leiomyosarcoma.
Asian Journal of Research in Zoology, 2021
Tunics hyperplasia of blood vessels can be quantified by a range of techniques that relate to mod... more Tunics hyperplasia of blood vessels can be quantified by a range of techniques that relate to modifications of area or thickness on histological sections. We studied hyperplasia of tunics of coronary arteries in a fish species, Rachycentron canadum for establishing the relationship between tunics and luminal obstruction by morphometry. The morphometric analysis was performed on 158 arterial vessels of 40 fish samples. The histological slides of cardiac and vascular tissue were stained using hematoxylin and eosin and observed under an optical microscope. The data relating to the proportion of the lumen area compared to the area of the tunic media were analyzed according to Beta regression models. The lumen proportion showed a decreasing relationship with weight and length of the analyzed animals. A linear relationship was not observed between lumen proportion and areas and/or radius of arterial tunics. It is concluded that the increase in weight gain contributes to a reduction of lum...
Annual Research & Review in Biology, 2020
In fish there is an innumerable variety of neoplasias that arise essentially from all cell types.... more In fish there is an innumerable variety of neoplasias that arise essentially from all cell types. Neolasia here, we will focus on the neoplasias that appear spontaneously in these animals and will not cover the experimentally induced neoplasias and/or the animal models of neoplasias. As for diagnosis, in general, specialists in aquatic organism pathology are not so familiar with the diagnosis of neoplasias. Infectious pathology, as opposed to non-infectious pathology, is the predominant condition in this area and, of course, these are of greater importance because some infectious diseases generate great economic losses, while neoplasias are isolated pathologies, with some exceptions. In the last 20 years, 10 neoplasias in different species have been diagnosed in our laboratory, and we reported their characteristics in this paper. We also made a detailed bibliography review and observed how 90 neoplasias among 56 species of teleosteal fish were reported. Neoplasias in fish, unlike ot...
Ultrastructural study of the thymus of Mugil liza Valenciennes, 1836 juveniles
Fish and Shellfish Immunology Reports, 2021
Brazilian Journal of Development, 2020
Thymus is considered a key component of the vertebrates' immune system. It is an organ that is on... more Thymus is considered a key component of the vertebrates' immune system. It is an organ that is only found in these animals whose development may be accompanied through the evolutionary scale, being considered as a facilitator and regulator in the interaction of lymphoid cells and lymphoid organs. In this study not only a morphological but also functional homology were demonstrated, in that it affects the immune system, of the humans and teleost fish thymus. The material of study was composed of the thymus tissue samples of humans who were donated by the
Aquaculture Research, 2020
Aquaculture is a farming activity under constant expansion. The sector has practically doubled it... more Aquaculture is a farming activity under constant expansion. The sector has practically doubled its annual production since 2000 (FAO, 2018). Brazil is one of the major producers of freshwater fish in the world and the fourth largest producer of Nile tilapia (Oreochromis niloticus) (IBGE, 2016). The species was introduced in Brazil in 1970, and due to its African origins, Nile tilapia has adapted well to the tropical climatic conditions of the country (Melo & Stipp, 2001). An important characteristic of the species is its ability to withstand water salinity levels, which renders its culture an attractive alternative, mainly in estuarine and coastal regions (Pereira et al., 2016). The growing expansion of aquaculture led to its intensification, which has been achieved through a reduction in production areas and an increase in fish stocking density. These practices ultimately result in decreased animal performance (Sánchez-Muros et al., 2016), which has in turn led to indiscriminate use of antibiotics as growth promoters. As a consequence, a number of problems have arisen regarding food safety in animal nutrition such as selection
Brazilian Journal of Veterinary Pathology, 2020
Fungal infections that affect marine mammals, like most fungal infections, can be secondary to en... more Fungal infections that affect marine mammals, like most fungal infections, can be secondary to environmental stress or other infectious diseases. This article describes a case of pulmonary infection by Aspergillus spp. in a specimen of Pontoporia blainvillei found dead near the town of San Clemente de Tuyu and near the bay of Samborombón, Buenos Aires, Argentina. The lung was collapsed with necrotic foci, fragments were collected, fixed in 10% formalin and submitted to histopathological examination. Microscopically, a marked change of the pulmonary architecture was observed, with total alveolar collapse, extensive necrotic areas, where septal hyphae were observed weakly, when stained with HE and clearly evidenced with Grocott staining. Since the potential causes of immunosuppression were not evident, the authors consider it probable that infection by Aspergillus spp. was established in the lungs that presented a primary, possibly bacterial pneumonia, as a result of some degree of immunosuppression.
Asian Journal of Research in Zoology, 2020
Lymphocystis disease has been reported worldwide in several species of freshwater fish and marine... more Lymphocystis disease has been reported worldwide in several species of freshwater fish and marine fish, naturally infected in the wild environment, or in intensive crops in farms. Nodular warty lesions of irregular surface were observed in the tegument and fins and mouth in a species of croaker (Micropogonias furnieri) caught in Cassino beach Rio Grande RS, Brazil and flounder (Paralichthys orbignyanus) caught in the city of San Clemente, Argentina. The skin lesions fragments were fixed in 20% buffered formalin, and the histological sections were stained with hematoxylin and eosin and Periodic Acid-Schiff (PAS), where microscopic alterations were visualized in the form of hyaline capsule with small basophilic structures in nodules and fibroblastic cells proliferation. The reported cases were based on the disease macroscopic findings characteristic of a lymphocystis disease, along with the histopathology, which confirmed the presence of the disease in the analyzed tissues.
Asian Journal of Medicine and Health, 2020
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Papers by Virgínia Pedrosa