Akrilamid (ACR) dünya çapında yaygın olarak tüketilen gıdalarda meydana gelen organik bir kimyasa... more Akrilamid (ACR) dünya çapında yaygın olarak tüketilen gıdalarda meydana gelen organik bir kimyasaldır. ACR reaktif oksijen moleküllerinin oluşumuna ve antioksidanların azalmasına yol açar. Bu çalışmanın amacı; karaciğer (KC), beyin ve böbrek dokusu total oksidan durum (TOS), antioksidan durum (TAS) ve KC ve beyin dokusu okside LDL (ox-LDL) düzeylerini uzun süre ACR + standart diyet ve ACR + yüksek yağlı diyet verilen sıçanlarda kontroller ile karşılaştırarak incelenmesidir. Toplam 48 adet 5-6 haftalık Wistar ırkı erkek sıçanlar iki diyet grubuna ayrılmış ve bir grup %20 yüksek yağ içerikli diyet ile diğer grup ise %2.7 standart yağ içerikli diyet ile beslenmiştir. Her iki diyet grubundaki hayvanlar 0, 2, 10 ve 20 mg/ kg/gün dozlarındaki ACR ile içme suları vasıtasıyla 28 gün boyunca muamele edilmiştir. Çalışma sonunda doku örneklerinde TAS, TOS veox-LDL analiz edilmiştir. ACR dozu yükseldikçe KC ve beyin ox-LDL/protein ve TOS/protein düzeyleri arttı. Ancak, 8 grup arasında KC dokusunda ox-LDL/protein (p=0.087) ve TOS/protein(p=0.751) düzeylerinde anlamlı fark yoktu. Beyin dokusunda ox-LDL/protein (p=0.808) seviyesinde anlamlı fark yok iken TOS/protein (p<0.001) seviyesinde anlamlı fark vardı. Böbrek dokusunda 8 grup arasında TOS/protein (p=0.052) seviyesinde anlamlı fark bulunamadı. Her üç dokuda ACR dozu yükseldikçe TAS/protein (p< 0.001) anlamlı olarak azaldığı tespit edildi. Sonuç olarak, 2, 10 ve 20 mg/kg dozlarında ACR + standart diyet ve ACR + yüksek yağlı diyet verilen sıçanlarda doku TOS ve ox-LDL seviyelerinde artış ve doku TAS seviyelerinde anlamlı azalma olduğunu göstermiştir. ACR oksidatif strese yol açmaktadır.
Acıbadem üniversitesi sağlık bilimleri dergisi, Jun 1, 2016
Metabolik Sedrom, dislipidemi, Tip 2 Diabetes Mellitus ve kardiovasküler hastalık gibi kronik has... more Metabolik Sedrom, dislipidemi, Tip 2 Diabetes Mellitus ve kardiovasküler hastalık gibi kronik hastalıklar için major risk faktörüdür. Son yıllarda obezitenin moleküler temelinin anlaşılmasında ilerlemelere rağmen, obezite'ye karşı kullanılan ilaçlar fizyolojik spesifite eksikliğine ve yan etkilere sahiptir. MicroRNA'lar (miRNA), kısa (~22 nt), kodlanamayan endojen RNA molekülleridir ve mRNA'nın 3' untranslated bölgesine bağlanarak birçok geni transkripsiyon sonrası düzenler. Bir miRNA birçok hedeflere sahiptir. Gelecekte miRNA'lar kronik hastalıklarının erken teşhisine yardımcı olacak ve yeni terapötik hedefler sağlayacaktır. miRNA'lar obezitede lipid metabolizması düzenlenmesinde bildirilmiştir. Bu derlemede adiposit biyolojisi ve obezite ile arasındaki ilgi, miRNA biyogenezi, düzenlenmesi, fonksiyonları ve obezitede lipid metabolizmasının düzenlenmesindeki rolü incelenecektir.
Aspartate aminotransferase (AST) macroenzyme leads to an increase in AST without the presence of ... more Aspartate aminotransferase (AST) macroenzyme leads to an increase in AST without the presence of any disease. In present report, an isolated increase of AST was detected in a 6-year old girl following the investigations prior to tonsillectomy. As to her history, physical examination and other laboratory test results, no abnormal findings were detected. AST levels were measured as 591 IU/L and 585 IU/L after repeated tests. While found as <5.4 IU/L (recovery <0.8%, reference interval 42.0-82.2%) after polyethylene glycol (PEG) precipitation, AST was determined as 561 IU/L (recovery 95.4%) as a result of the assessment performed with non-specific human antimouse antibodies (HAMA). No diseases were encountered in the patient, and reason for the increase of AST was considered to be macroAST. Because continuously increased enzyme value may lead to various invasive and expensive diagnostic tests, macroAST should be taken into account in the evaluation process.
Infertility is described as the absence of pregnancy after one year of regular sexual intercourse... more Infertility is described as the absence of pregnancy after one year of regular sexual intercourse. It affects approximately 12%-15% of couples, with male infertility accounting for 40% of that number. The aetiopathogenesis of male infertility is not fully known; intensive research is underway to clarify this multifactorial condition (Blomberg
To evaluate the relationship between serum calprotectin and oxidized low density lipoprotein (LDL... more To evaluate the relationship between serum calprotectin and oxidized low density lipoprotein (LDL) in preeclamptic patients. Additionally, we aimed to measure serum malondialdehyde (MDA) levels as an oxidative stress marker. Materials and Methods: Serum calprotectin, oxidized LDL, and MDA levels were measured in a crosssectional study of 61 preeclamptic patients (39 severe preeclampsia and 22 mild preeclampsia cases) and 20 healthy pregnant subjects. Serum MDA concentration was measured with spectrophotometric methods, and oxidized LDL and calprotectin were measured via enzyme-linked immunoassay (ELISA). Results: Serum calprotectin (P < 0.0001 and P = 0.014) and MDA (P = 0.001 and P = 0.028) levels were significantly higher in the severe and mild preeclampsia groups than in the healthy control group. Serum oxidized LDL concentration was slightly higher, but not did reach a statistically significant level, in the severe preeclampsia group than in the healthy pregnant group (P = 0.2). There was a significant negative correlation between the levels of calprotectin and oxidized LDL (r = -0.34, P = 0.006) in the mild and severe preeclampsia groups. The correlation between calprotectin and MDA was not statistically significant (P = 0.08). The findings suggest that increased calprotectin is associated with preeclampsia. Further study is necessary in order to determine whether or not high calprotectin levels prevent oxidation of circulating LDL in preeclampsia cases.
International Journal for Vitamin and Nutrition Research, Nov 1, 2019
. Vitamin D deficiency is associated with several non-homeostatic conditions and/or diseases like... more . Vitamin D deficiency is associated with several non-homeostatic conditions and/or diseases like inflammation, atherosclerosis, cardiovascular disease and mortality. YKL-40 is a glycoprotein, secreted by macrophages, neutrophils and different cell types and it is also associated with inflammation and pathological tissue remodeling. In this study, we aimed to evaluate relationship between the proinflammatory biomarkers YKL-40 and hs-CRP levels and vitamin D deficiency. Our study group includes 45 subjects with vitamin D deficiency (Group 1) (20 M, 25 F; mean age 37.72 ± 7.70 years) and 40 age and sex-matched healthy subjects with normal serum levels of vitamin D (Group 2) (19 M, 21 F; mean age 39.26 ± 7.41 years). Plasma 25 (OH) vitamin D levels were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. Plasma YKL-40 analysis was performed by ELISA. Serum hs-CRP levels were measured by nephelometric method. Plasma vitamin D levels below 20 ng/mL were accepted as vitamin D deficiency. Although we could not find any significant differences by means of serum hs-CRP levels between Group 1 and Group 2 (2.21 (0.27–11.70); 1.79 (0.16–9.85) mg/L, p = 0.247), plasma YKL-40 levels were significantly higher in group 1 than group2 (70.47 (17.84–198.50); 47.14 (4.80–135.48) ng/mL, p = 0.047). In literature, vitamin D deficiency is associated with inflammation. In our study, we found similar hs-CRP levels between groups and higher YKL-40 levels in group 1. Vitamin D deficiency may be related to high YKL-40 levels in terms of causing chronic inflammation.
DOAJ (DOAJ: Directory of Open Access Journals), Mar 1, 2017
Background: Upon diagnosis, Pulmonary Tuberculosis patients are treated for TB for a period of 6-... more Background: Upon diagnosis, Pulmonary Tuberculosis patients are treated for TB for a period of 6-9 months. At present, there exists very little indication of the efficacy of the particular treatment. A few previous studies have shown that soluble urokinase plasminogen activator receptor (SuPAR) may be used as treatment efficacy marker. SuPAR is a cellular receptor for serine protease urokinase plasminogen activator (uPA). Bacterial endotoxins and cytokines of the innate immune system stimulate the secretion of uPA in monocytes & neutrophils. Serum SuPAR levels are elevated when TB is active and decrease when the patient responds positively to therapy. Objective: To investigate if SuPAR levels decline upon treatment and whether serum SuPAR levels may be used as a biomarker to monitor Tuberculosis treatment efficacy. Design: The study was conducted in the department of Biochemistry at VIMS, Ballari, Karnataka. The study subjects were randomly selected from RNTCP centre of VIMS. Controls: Twelve tuberculin skin test positive healthy controls from the community. Cases: A total of 60 cases were enrolled for the study and were divided into 3 groups with 20 in each, based on the duration of TB treatment. Group I (n = 20): Newly diagnosed pulmonary TB patients before initiation of DOTS. Group II (n = 20): TB patients, 2-3 months after initiation of DOTS. Group III (n = 20): TB patients who had completed 6 months of DOTS. Methodology: Hb%, TC, DC(P)%, DC(L)% & ESR were measured by standard procedures. Serum suPAR was measured by the quantitative sandwich enzyme immunoassay technique using the R & D systems Human uPAR Quantikine ELISA Kit. Results: The suPAR levels were elevated before treatment (3.27 + 2.08 ng/ml) and dropped significantly in groups after 2 months of initiation of therapy (2.18 + 1.17 ng/ml) and after completion of 6 months of treatment (1.50 + 0.93 ng/ml). Conclusion: The decrease in suPAR levels in PTB patients with treatment is a manifestation of treatment efficacy. Hence suPAR levels can be used to guide clinical decisions in TB management.
Background and aim: Acute pancreatitis (AP), an inflammatory disorder of the pancreas, is associa... more Background and aim: Acute pancreatitis (AP), an inflammatory disorder of the pancreas, is associated with significant morbidity and mortality. The pathogenesis of AP has been suggested to -involve high oxidative stress (OS), combined with inadequate antioxidant status. We aimed to investigate the levels of serum total anti-oxidant status (TAS), total oxidant status (TOS) and ischemia-modified albumin (IMA) in patients with mild AP2016. Methods: Thirty subjects with mild AP and 29 healthy controls were enrolled into the study. The levels of TAS, TOS and IMA, C-reactive protein (CRP), high sensitivity CRP (hs-CRP) and fibrinogen were measured in both groups. Results: TAS levels were significantly lower (p = 0.037), while IMA levels were significantly higher (p < 0.001) in patients, compared to controls. TOS levels were similar between two groups. Fibrinogen, CRP and hs-CRP levels were significantly higher in patients than those of controls (p < 0.001 for all parameters). IMA levels were positively correlated with amylase and lipase levels (r = 0.448, p = 0.001 and r = 0.469, p < 0.001, respectively). There was a negative correlation between TAS levels, and amylase and lipase levels (r = -0.277, p = 0.035 and r = -0.278, p = 0.034, respectively). Conclusion: OS is reported to be associated with the inflammatory process and the severity of AP. In our study, among OS parameters, an increase in IMA levels and a decrease in TAS levels were observed in mild AP patients.
Objective: Thyroid hormones are closely associated with cardiac autonomic functions. Both hypothy... more Objective: Thyroid hormones are closely associated with cardiac autonomic functions. Both hypothyroidism and hyperthyroidism may be associated with bradyarrhymia including atrioventricular block. We aimed to evaluate the incidence and outcome of thyroid dysfunction in a bradyarrhymia cohort in a tertiary center. Methods: This study included patients hospitalized for atrioventricular block and bradyarrhymia between 2008-2011 in a tertiary reference hospital were evaluated retrospectively. The levels of throid levels during hospitalisation were recorded. Patients were grouped based on thyroid status. The relationship between thyroid hormone status and AV block was evaluated. Patients were followed up for requirement of permanent pacemaker. Results: Mean age of the patients were 68,2 ± 15,4. Of the patients evaluated 92.6% of the patients were euthyroid state and 7.4 % of the patients had thyroid dysfunction. 72 percent of the patients were diagnosed during index hospitalisation for thyroid dysfunction. The site of the atrioventricular block(AV-Nodal, infra-nodal-undetermined) were not different between groups.The reversibility of the AV block were more common in patients with diabetes mellitus, hypertension and hypothyroidism. %80 of the patients were required to implant pacemaker during hospitalisation. Conclusions: The presence of the thyroid dysfunction was commonly encountered in the clinical practice and the rate of permanent pacemaker implantation was similar to euthyroid patients in our chort. Further studies are needed to interpret the impact of thyroid fuction in these patients.
Background: Childhood obesity is an increasing health challenge related to increased risk of chro... more Background: Childhood obesity is an increasing health challenge related to increased risk of chronic diseases. microRNAs (miRNAs) are noncoding short RNA molecules regulating multiple biological processes linked to obesity. Objectives: We aimed at evaluating the association between circulating miRNA levels and lipid metabolism in obese and non-obese children and adolescents. Methods: By constituting study group, 45 obese children and adolescents were recruited. To perform comparisons with study group, 41 lean controls were matched for age and sex. Using real-time quantitative PCR analysis, circulating miRNAs were evaluated in both groups. Results: Circulating miR-335 (P < 0.001), miR-143 (P = 0.001) and miR-758 (P = 0.006) in obese children were significantly lower than those of controls. However, circulating miR-27 (P = 0.032), miR-378 (P < 0.001) and miR-370 (P = 0.045) in obese children were significantly higher, compared with those of controls. In addition, circulating miR-33 in obese children was higher than those of controls, but no significant difference was present (P = 0.687). Conclusion: Our findings showed that a significant association is present between circulating miR-370, miR-33, miR-378, miR-27, miR-335, miR-143 and miR-758 values, and childhood obesity. Low levels of miR-335, miR-143 and miR-758, and high levels of miR-27, miR-378, miR-33 and miR-370 may have been responsible for elevated triglycerides and low-density lipoprotein (LDL-C) levels, and low level of high-density lipoprotein (HDL-C) in obese subjects. Therefore, miRNAs may be a good novel biomarker for childhood obesity.
İskemi modifiye albumin iskemi sonucu oluşan reaktif oksijen türevlerinin yol açtığı albuminde mo... more İskemi modifiye albumin iskemi sonucu oluşan reaktif oksijen türevlerinin yol açtığı albuminde modifikasyon ile oluşur. Albumin kobalt bağlama testi kullanılarak ölçülür. Bu test geçici olmayan kardiyak hasarın başlangıcından önce iskeminin erken saptanmasında kullanılır. Bu yazıda iskemi modifiye albuminin patofizyolojisi, analizi ve klinik uygulamalarını inceledik.
Revista Da Associacao Medica Brasileira, Jun 1, 2022
OBJECTIVE: Hypertension is a major modifiable risk factor for cardiovascular disease and prematur... more OBJECTIVE: Hypertension is a major modifiable risk factor for cardiovascular disease and premature death worldwide. Phoenixin is a newly identified neuropeptide with multiple bioactivity. However, there was no published data about phoenixin levels in hypertension. The aim of this study was to evaluate the relationship between phoenixin and hypertension. METHODS: This study was performed in 36 patients with hypertension and 36 healthy controls. Serum phoenixin-14 and phoenixin-20 levels were determined by Enzyme-Linked ImmunoSorbent Assay method. RESULTS: Serum phoenixin-14 and phoenixin-20 values were significantly lower in hypertension patients compared with the control group (p<0.001). The levels of phoenixin-14 were negatively correlated with weight (r=-0.376; p<0.005), body mass index (r=-0.407; p<0.001), systolic blood pressure (r=-0.586; p<0.001), and diastolic blood pressure (r=-0.319; p<0.01). There was a negative correlation between serum phoenixin-20 and weight (r=-0.378; p<0.005), body mass index (r=-0.383; p<0.005), systolic blood pressure (r=-0.551; p<0.001), and diastolic blood pressure (r=-0.306; p<0.01). We used receiver operating characteristic curve analyses to compare the diagnosis value of Phoenixin-14 and Phoenixin-20 levels in hypertensive patients. We found that Phoenixin-14 value is an area under the curve of 0.87 (cutoff value 404.7 ng/L, sensitivity 92%, specificity 72%) and Phoenixin-20 value is an area under the curve of 0.83 (cutoff value 209.9 ng/L, sensitivity 86%, specificity 75%). Phoenixin-14 did nearly show equally compared to phoenixin-20 in predicting hypertension. CONCLUSION: Serum phoenixin-14 and phoenixin-20 may be related to the pathogenesis of hypertension. Our findings indicated that serum phoenixin-14 and phoenixin-20 may serve as a novel biomarker for the diagnosis of hypertension.
Tip 1 diyabet mellitus metabolik bir hastalık olup, patogenezinde oksidatif stres rol oynamaktadi... more Tip 1 diyabet mellitus metabolik bir hastalık olup, patogenezinde oksidatif stres rol oynamaktadir. Oksidatif stres serbest oksijen radikalleri ve lipid peroksidlerinin artışı ve antioksidan kapasitesinin azalmasına bağlıdır. Bu çalışmada tip 1 diyabet mellitus'da oksidatif stresin etkisini değerlendirmek için serum, lökösit ve eritrosit lipid peroksidasyonu, ve lökösit ve eritrosit glutatyon peroksidaz ve süperoksid dizmutaz aktivitelerine ilaveten serum ve lökösit vitamin C seviyelerini sağlıklı kontroller ile karşılaştırdık. Yöntem: Çalışmaya hasta grubu olarak 34 çocuk ve adolesan (20 kız ve 14 erkek) ve kontrol grubu 29 çocuk ve adölesan (13 kız ve 16 erkek) dahil edildi. Lipid peroksidasyonunun son ürünü malondialdehid, tiyobarbitürik asid reaktivite yöntemi, vitamin C seviyeleri dinitrofenilhidrazin metodu ile ölçüldü. Glutatyon peroksidaz ve süperoksid dizmutaz düzeyleri ticari kit kullanılarak tesbit edildi. Analiz sonuçlarının istatiksel analizleri SPSS 20.0 bilgisayar proğramı ile yapıldı. Bulgular: Tip 1 diyabet mellitus çocuk ve adölesanlar kontroller ile karşılaştırıldığında serum, lökosit ve eritrosit malondialdehid seviyeleri artmasına karşın lökosit ve eritrosit glutatyon peroksidaz ve süperoksid dizmutaz aktiviteleri, ve serum ve lökosit vitamin C seviyelerinde azalma tesbit edilmişti. Fakat bu değişikliklerde istatistiksel olarak anlamlı fark gözlenmedi (p > 0.05). Bu parametreler ile HbA1c ve glukoz seviyeleri arasında korelasyon yoktu. Sonuç: Tip I diyabet mellitus cocuk ve adolesanlarda lipid peroksidasyon artışına ve antioksidan sisteminde azalmaya eğilim olmakla birlikte bu durumun düzenli kullanılan insülin tedavisi ile düzeltildiği ve diğer faktörler tarafında etkilendiği kanaatindeyiz.
Background: Vascular calcification has been found to be associated with increased risk of cardiov... more Background: Vascular calcification has been found to be associated with increased risk of cardiovascular (CV) morbidity and mortality. Various bone-associated proteins has been suggested to be related with this process. In this study, we aimed to evaluate whether serum levels of bone morphogenic protein-4 (BMP-4) and matrix Gla protein (MGP) differed in patients who were found to have normal epicardial coronary arteries or a culprit lesion in the coronary angiography leading to acute coronary syndrome (ACS). Methods: Patients admitted to emergency department with the diagnosis of ACS and underwent primary percutaneous coronary intervention between October 2015 e April 2016 were consecutively recruited as the patient group. Age and gender-matched subjects who underwent coronary angiography following non-invasive ischemia assessment made the control group. Results: A total of 90 subjects (63.00AE 14.02 years, 70% male) were included in this study. MGP (<0.001) and BMP-4 (<0.001) levels were significantly elevated when compared to subjects with normal coronary arteries. Fasting blood glucose (p¼ 0.024), HDL-cholesterol(p¼ 0.002), CRP (p¼ 0.001) levels and LVEF (p¼ 0.021) were significantly correlated with serum MGP levels. HDL-cholesterol (p¼ 0.001) and CRP (p¼ 0.030) levels were also significantly correlated with serum BMP-4 levels. In the model including HDL-cholesterol, CRP, MGP, and BMP-4, only MGP (odds ratio[OR]: 1.018, p¼ 0.019) and BMP-4 (OR: 1.313, p¼ 0.023) were found to be independently associated with ACS. Conclusion: This study shows that serum BMP-4 and MGP are independently associated with ACS occurrence, when adjusted for other CV risk factors. These biomarkers may have a diagnostic potential in ACS patients.
Akrilamid (ACR) dünya çapında yaygın olarak tüketilen gıdalarda meydana gelen organik bir kimyasa... more Akrilamid (ACR) dünya çapında yaygın olarak tüketilen gıdalarda meydana gelen organik bir kimyasaldır. ACR reaktif oksijen moleküllerinin oluşumuna ve antioksidanların azalmasına yol açar. Bu çalışmanın amacı; karaciğer (KC), beyin ve böbrek dokusu total oksidan durum (TOS), antioksidan durum (TAS) ve KC ve beyin dokusu okside LDL (ox-LDL) düzeylerini uzun süre ACR + standart diyet ve ACR + yüksek yağlı diyet verilen sıçanlarda kontroller ile karşılaştırarak incelenmesidir. Toplam 48 adet 5-6 haftalık Wistar ırkı erkek sıçanlar iki diyet grubuna ayrılmış ve bir grup %20 yüksek yağ içerikli diyet ile diğer grup ise %2.7 standart yağ içerikli diyet ile beslenmiştir. Her iki diyet grubundaki hayvanlar 0, 2, 10 ve 20 mg/ kg/gün dozlarındaki ACR ile içme suları vasıtasıyla 28 gün boyunca muamele edilmiştir. Çalışma sonunda doku örneklerinde TAS, TOS veox-LDL analiz edilmiştir. ACR dozu yükseldikçe KC ve beyin ox-LDL/protein ve TOS/protein düzeyleri arttı. Ancak, 8 grup arasında KC dokusunda ox-LDL/protein (p=0.087) ve TOS/protein(p=0.751) düzeylerinde anlamlı fark yoktu. Beyin dokusunda ox-LDL/protein (p=0.808) seviyesinde anlamlı fark yok iken TOS/protein (p<0.001) seviyesinde anlamlı fark vardı. Böbrek dokusunda 8 grup arasında TOS/protein (p=0.052) seviyesinde anlamlı fark bulunamadı. Her üç dokuda ACR dozu yükseldikçe TAS/protein (p< 0.001) anlamlı olarak azaldığı tespit edildi. Sonuç olarak, 2, 10 ve 20 mg/kg dozlarında ACR + standart diyet ve ACR + yüksek yağlı diyet verilen sıçanlarda doku TOS ve ox-LDL seviyelerinde artış ve doku TAS seviyelerinde anlamlı azalma olduğunu göstermiştir. ACR oksidatif strese yol açmaktadır.
Acıbadem üniversitesi sağlık bilimleri dergisi, Jun 1, 2016
Metabolik Sedrom, dislipidemi, Tip 2 Diabetes Mellitus ve kardiovasküler hastalık gibi kronik has... more Metabolik Sedrom, dislipidemi, Tip 2 Diabetes Mellitus ve kardiovasküler hastalık gibi kronik hastalıklar için major risk faktörüdür. Son yıllarda obezitenin moleküler temelinin anlaşılmasında ilerlemelere rağmen, obezite'ye karşı kullanılan ilaçlar fizyolojik spesifite eksikliğine ve yan etkilere sahiptir. MicroRNA'lar (miRNA), kısa (~22 nt), kodlanamayan endojen RNA molekülleridir ve mRNA'nın 3' untranslated bölgesine bağlanarak birçok geni transkripsiyon sonrası düzenler. Bir miRNA birçok hedeflere sahiptir. Gelecekte miRNA'lar kronik hastalıklarının erken teşhisine yardımcı olacak ve yeni terapötik hedefler sağlayacaktır. miRNA'lar obezitede lipid metabolizması düzenlenmesinde bildirilmiştir. Bu derlemede adiposit biyolojisi ve obezite ile arasındaki ilgi, miRNA biyogenezi, düzenlenmesi, fonksiyonları ve obezitede lipid metabolizmasının düzenlenmesindeki rolü incelenecektir.
Aspartate aminotransferase (AST) macroenzyme leads to an increase in AST without the presence of ... more Aspartate aminotransferase (AST) macroenzyme leads to an increase in AST without the presence of any disease. In present report, an isolated increase of AST was detected in a 6-year old girl following the investigations prior to tonsillectomy. As to her history, physical examination and other laboratory test results, no abnormal findings were detected. AST levels were measured as 591 IU/L and 585 IU/L after repeated tests. While found as <5.4 IU/L (recovery <0.8%, reference interval 42.0-82.2%) after polyethylene glycol (PEG) precipitation, AST was determined as 561 IU/L (recovery 95.4%) as a result of the assessment performed with non-specific human antimouse antibodies (HAMA). No diseases were encountered in the patient, and reason for the increase of AST was considered to be macroAST. Because continuously increased enzyme value may lead to various invasive and expensive diagnostic tests, macroAST should be taken into account in the evaluation process.
Infertility is described as the absence of pregnancy after one year of regular sexual intercourse... more Infertility is described as the absence of pregnancy after one year of regular sexual intercourse. It affects approximately 12%-15% of couples, with male infertility accounting for 40% of that number. The aetiopathogenesis of male infertility is not fully known; intensive research is underway to clarify this multifactorial condition (Blomberg
To evaluate the relationship between serum calprotectin and oxidized low density lipoprotein (LDL... more To evaluate the relationship between serum calprotectin and oxidized low density lipoprotein (LDL) in preeclamptic patients. Additionally, we aimed to measure serum malondialdehyde (MDA) levels as an oxidative stress marker. Materials and Methods: Serum calprotectin, oxidized LDL, and MDA levels were measured in a crosssectional study of 61 preeclamptic patients (39 severe preeclampsia and 22 mild preeclampsia cases) and 20 healthy pregnant subjects. Serum MDA concentration was measured with spectrophotometric methods, and oxidized LDL and calprotectin were measured via enzyme-linked immunoassay (ELISA). Results: Serum calprotectin (P < 0.0001 and P = 0.014) and MDA (P = 0.001 and P = 0.028) levels were significantly higher in the severe and mild preeclampsia groups than in the healthy control group. Serum oxidized LDL concentration was slightly higher, but not did reach a statistically significant level, in the severe preeclampsia group than in the healthy pregnant group (P = 0.2). There was a significant negative correlation between the levels of calprotectin and oxidized LDL (r = -0.34, P = 0.006) in the mild and severe preeclampsia groups. The correlation between calprotectin and MDA was not statistically significant (P = 0.08). The findings suggest that increased calprotectin is associated with preeclampsia. Further study is necessary in order to determine whether or not high calprotectin levels prevent oxidation of circulating LDL in preeclampsia cases.
International Journal for Vitamin and Nutrition Research, Nov 1, 2019
. Vitamin D deficiency is associated with several non-homeostatic conditions and/or diseases like... more . Vitamin D deficiency is associated with several non-homeostatic conditions and/or diseases like inflammation, atherosclerosis, cardiovascular disease and mortality. YKL-40 is a glycoprotein, secreted by macrophages, neutrophils and different cell types and it is also associated with inflammation and pathological tissue remodeling. In this study, we aimed to evaluate relationship between the proinflammatory biomarkers YKL-40 and hs-CRP levels and vitamin D deficiency. Our study group includes 45 subjects with vitamin D deficiency (Group 1) (20 M, 25 F; mean age 37.72 ± 7.70 years) and 40 age and sex-matched healthy subjects with normal serum levels of vitamin D (Group 2) (19 M, 21 F; mean age 39.26 ± 7.41 years). Plasma 25 (OH) vitamin D levels were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. Plasma YKL-40 analysis was performed by ELISA. Serum hs-CRP levels were measured by nephelometric method. Plasma vitamin D levels below 20 ng/mL were accepted as vitamin D deficiency. Although we could not find any significant differences by means of serum hs-CRP levels between Group 1 and Group 2 (2.21 (0.27–11.70); 1.79 (0.16–9.85) mg/L, p = 0.247), plasma YKL-40 levels were significantly higher in group 1 than group2 (70.47 (17.84–198.50); 47.14 (4.80–135.48) ng/mL, p = 0.047). In literature, vitamin D deficiency is associated with inflammation. In our study, we found similar hs-CRP levels between groups and higher YKL-40 levels in group 1. Vitamin D deficiency may be related to high YKL-40 levels in terms of causing chronic inflammation.
DOAJ (DOAJ: Directory of Open Access Journals), Mar 1, 2017
Background: Upon diagnosis, Pulmonary Tuberculosis patients are treated for TB for a period of 6-... more Background: Upon diagnosis, Pulmonary Tuberculosis patients are treated for TB for a period of 6-9 months. At present, there exists very little indication of the efficacy of the particular treatment. A few previous studies have shown that soluble urokinase plasminogen activator receptor (SuPAR) may be used as treatment efficacy marker. SuPAR is a cellular receptor for serine protease urokinase plasminogen activator (uPA). Bacterial endotoxins and cytokines of the innate immune system stimulate the secretion of uPA in monocytes & neutrophils. Serum SuPAR levels are elevated when TB is active and decrease when the patient responds positively to therapy. Objective: To investigate if SuPAR levels decline upon treatment and whether serum SuPAR levels may be used as a biomarker to monitor Tuberculosis treatment efficacy. Design: The study was conducted in the department of Biochemistry at VIMS, Ballari, Karnataka. The study subjects were randomly selected from RNTCP centre of VIMS. Controls: Twelve tuberculin skin test positive healthy controls from the community. Cases: A total of 60 cases were enrolled for the study and were divided into 3 groups with 20 in each, based on the duration of TB treatment. Group I (n = 20): Newly diagnosed pulmonary TB patients before initiation of DOTS. Group II (n = 20): TB patients, 2-3 months after initiation of DOTS. Group III (n = 20): TB patients who had completed 6 months of DOTS. Methodology: Hb%, TC, DC(P)%, DC(L)% & ESR were measured by standard procedures. Serum suPAR was measured by the quantitative sandwich enzyme immunoassay technique using the R & D systems Human uPAR Quantikine ELISA Kit. Results: The suPAR levels were elevated before treatment (3.27 + 2.08 ng/ml) and dropped significantly in groups after 2 months of initiation of therapy (2.18 + 1.17 ng/ml) and after completion of 6 months of treatment (1.50 + 0.93 ng/ml). Conclusion: The decrease in suPAR levels in PTB patients with treatment is a manifestation of treatment efficacy. Hence suPAR levels can be used to guide clinical decisions in TB management.
Background and aim: Acute pancreatitis (AP), an inflammatory disorder of the pancreas, is associa... more Background and aim: Acute pancreatitis (AP), an inflammatory disorder of the pancreas, is associated with significant morbidity and mortality. The pathogenesis of AP has been suggested to -involve high oxidative stress (OS), combined with inadequate antioxidant status. We aimed to investigate the levels of serum total anti-oxidant status (TAS), total oxidant status (TOS) and ischemia-modified albumin (IMA) in patients with mild AP2016. Methods: Thirty subjects with mild AP and 29 healthy controls were enrolled into the study. The levels of TAS, TOS and IMA, C-reactive protein (CRP), high sensitivity CRP (hs-CRP) and fibrinogen were measured in both groups. Results: TAS levels were significantly lower (p = 0.037), while IMA levels were significantly higher (p < 0.001) in patients, compared to controls. TOS levels were similar between two groups. Fibrinogen, CRP and hs-CRP levels were significantly higher in patients than those of controls (p < 0.001 for all parameters). IMA levels were positively correlated with amylase and lipase levels (r = 0.448, p = 0.001 and r = 0.469, p < 0.001, respectively). There was a negative correlation between TAS levels, and amylase and lipase levels (r = -0.277, p = 0.035 and r = -0.278, p = 0.034, respectively). Conclusion: OS is reported to be associated with the inflammatory process and the severity of AP. In our study, among OS parameters, an increase in IMA levels and a decrease in TAS levels were observed in mild AP patients.
Objective: Thyroid hormones are closely associated with cardiac autonomic functions. Both hypothy... more Objective: Thyroid hormones are closely associated with cardiac autonomic functions. Both hypothyroidism and hyperthyroidism may be associated with bradyarrhymia including atrioventricular block. We aimed to evaluate the incidence and outcome of thyroid dysfunction in a bradyarrhymia cohort in a tertiary center. Methods: This study included patients hospitalized for atrioventricular block and bradyarrhymia between 2008-2011 in a tertiary reference hospital were evaluated retrospectively. The levels of throid levels during hospitalisation were recorded. Patients were grouped based on thyroid status. The relationship between thyroid hormone status and AV block was evaluated. Patients were followed up for requirement of permanent pacemaker. Results: Mean age of the patients were 68,2 ± 15,4. Of the patients evaluated 92.6% of the patients were euthyroid state and 7.4 % of the patients had thyroid dysfunction. 72 percent of the patients were diagnosed during index hospitalisation for thyroid dysfunction. The site of the atrioventricular block(AV-Nodal, infra-nodal-undetermined) were not different between groups.The reversibility of the AV block were more common in patients with diabetes mellitus, hypertension and hypothyroidism. %80 of the patients were required to implant pacemaker during hospitalisation. Conclusions: The presence of the thyroid dysfunction was commonly encountered in the clinical practice and the rate of permanent pacemaker implantation was similar to euthyroid patients in our chort. Further studies are needed to interpret the impact of thyroid fuction in these patients.
Background: Childhood obesity is an increasing health challenge related to increased risk of chro... more Background: Childhood obesity is an increasing health challenge related to increased risk of chronic diseases. microRNAs (miRNAs) are noncoding short RNA molecules regulating multiple biological processes linked to obesity. Objectives: We aimed at evaluating the association between circulating miRNA levels and lipid metabolism in obese and non-obese children and adolescents. Methods: By constituting study group, 45 obese children and adolescents were recruited. To perform comparisons with study group, 41 lean controls were matched for age and sex. Using real-time quantitative PCR analysis, circulating miRNAs were evaluated in both groups. Results: Circulating miR-335 (P < 0.001), miR-143 (P = 0.001) and miR-758 (P = 0.006) in obese children were significantly lower than those of controls. However, circulating miR-27 (P = 0.032), miR-378 (P < 0.001) and miR-370 (P = 0.045) in obese children were significantly higher, compared with those of controls. In addition, circulating miR-33 in obese children was higher than those of controls, but no significant difference was present (P = 0.687). Conclusion: Our findings showed that a significant association is present between circulating miR-370, miR-33, miR-378, miR-27, miR-335, miR-143 and miR-758 values, and childhood obesity. Low levels of miR-335, miR-143 and miR-758, and high levels of miR-27, miR-378, miR-33 and miR-370 may have been responsible for elevated triglycerides and low-density lipoprotein (LDL-C) levels, and low level of high-density lipoprotein (HDL-C) in obese subjects. Therefore, miRNAs may be a good novel biomarker for childhood obesity.
İskemi modifiye albumin iskemi sonucu oluşan reaktif oksijen türevlerinin yol açtığı albuminde mo... more İskemi modifiye albumin iskemi sonucu oluşan reaktif oksijen türevlerinin yol açtığı albuminde modifikasyon ile oluşur. Albumin kobalt bağlama testi kullanılarak ölçülür. Bu test geçici olmayan kardiyak hasarın başlangıcından önce iskeminin erken saptanmasında kullanılır. Bu yazıda iskemi modifiye albuminin patofizyolojisi, analizi ve klinik uygulamalarını inceledik.
Revista Da Associacao Medica Brasileira, Jun 1, 2022
OBJECTIVE: Hypertension is a major modifiable risk factor for cardiovascular disease and prematur... more OBJECTIVE: Hypertension is a major modifiable risk factor for cardiovascular disease and premature death worldwide. Phoenixin is a newly identified neuropeptide with multiple bioactivity. However, there was no published data about phoenixin levels in hypertension. The aim of this study was to evaluate the relationship between phoenixin and hypertension. METHODS: This study was performed in 36 patients with hypertension and 36 healthy controls. Serum phoenixin-14 and phoenixin-20 levels were determined by Enzyme-Linked ImmunoSorbent Assay method. RESULTS: Serum phoenixin-14 and phoenixin-20 values were significantly lower in hypertension patients compared with the control group (p<0.001). The levels of phoenixin-14 were negatively correlated with weight (r=-0.376; p<0.005), body mass index (r=-0.407; p<0.001), systolic blood pressure (r=-0.586; p<0.001), and diastolic blood pressure (r=-0.319; p<0.01). There was a negative correlation between serum phoenixin-20 and weight (r=-0.378; p<0.005), body mass index (r=-0.383; p<0.005), systolic blood pressure (r=-0.551; p<0.001), and diastolic blood pressure (r=-0.306; p<0.01). We used receiver operating characteristic curve analyses to compare the diagnosis value of Phoenixin-14 and Phoenixin-20 levels in hypertensive patients. We found that Phoenixin-14 value is an area under the curve of 0.87 (cutoff value 404.7 ng/L, sensitivity 92%, specificity 72%) and Phoenixin-20 value is an area under the curve of 0.83 (cutoff value 209.9 ng/L, sensitivity 86%, specificity 75%). Phoenixin-14 did nearly show equally compared to phoenixin-20 in predicting hypertension. CONCLUSION: Serum phoenixin-14 and phoenixin-20 may be related to the pathogenesis of hypertension. Our findings indicated that serum phoenixin-14 and phoenixin-20 may serve as a novel biomarker for the diagnosis of hypertension.
Tip 1 diyabet mellitus metabolik bir hastalık olup, patogenezinde oksidatif stres rol oynamaktadi... more Tip 1 diyabet mellitus metabolik bir hastalık olup, patogenezinde oksidatif stres rol oynamaktadir. Oksidatif stres serbest oksijen radikalleri ve lipid peroksidlerinin artışı ve antioksidan kapasitesinin azalmasına bağlıdır. Bu çalışmada tip 1 diyabet mellitus'da oksidatif stresin etkisini değerlendirmek için serum, lökösit ve eritrosit lipid peroksidasyonu, ve lökösit ve eritrosit glutatyon peroksidaz ve süperoksid dizmutaz aktivitelerine ilaveten serum ve lökösit vitamin C seviyelerini sağlıklı kontroller ile karşılaştırdık. Yöntem: Çalışmaya hasta grubu olarak 34 çocuk ve adolesan (20 kız ve 14 erkek) ve kontrol grubu 29 çocuk ve adölesan (13 kız ve 16 erkek) dahil edildi. Lipid peroksidasyonunun son ürünü malondialdehid, tiyobarbitürik asid reaktivite yöntemi, vitamin C seviyeleri dinitrofenilhidrazin metodu ile ölçüldü. Glutatyon peroksidaz ve süperoksid dizmutaz düzeyleri ticari kit kullanılarak tesbit edildi. Analiz sonuçlarının istatiksel analizleri SPSS 20.0 bilgisayar proğramı ile yapıldı. Bulgular: Tip 1 diyabet mellitus çocuk ve adölesanlar kontroller ile karşılaştırıldığında serum, lökosit ve eritrosit malondialdehid seviyeleri artmasına karşın lökosit ve eritrosit glutatyon peroksidaz ve süperoksid dizmutaz aktiviteleri, ve serum ve lökosit vitamin C seviyelerinde azalma tesbit edilmişti. Fakat bu değişikliklerde istatistiksel olarak anlamlı fark gözlenmedi (p > 0.05). Bu parametreler ile HbA1c ve glukoz seviyeleri arasında korelasyon yoktu. Sonuç: Tip I diyabet mellitus cocuk ve adolesanlarda lipid peroksidasyon artışına ve antioksidan sisteminde azalmaya eğilim olmakla birlikte bu durumun düzenli kullanılan insülin tedavisi ile düzeltildiği ve diğer faktörler tarafında etkilendiği kanaatindeyiz.
Background: Vascular calcification has been found to be associated with increased risk of cardiov... more Background: Vascular calcification has been found to be associated with increased risk of cardiovascular (CV) morbidity and mortality. Various bone-associated proteins has been suggested to be related with this process. In this study, we aimed to evaluate whether serum levels of bone morphogenic protein-4 (BMP-4) and matrix Gla protein (MGP) differed in patients who were found to have normal epicardial coronary arteries or a culprit lesion in the coronary angiography leading to acute coronary syndrome (ACS). Methods: Patients admitted to emergency department with the diagnosis of ACS and underwent primary percutaneous coronary intervention between October 2015 e April 2016 were consecutively recruited as the patient group. Age and gender-matched subjects who underwent coronary angiography following non-invasive ischemia assessment made the control group. Results: A total of 90 subjects (63.00AE 14.02 years, 70% male) were included in this study. MGP (<0.001) and BMP-4 (<0.001) levels were significantly elevated when compared to subjects with normal coronary arteries. Fasting blood glucose (p¼ 0.024), HDL-cholesterol(p¼ 0.002), CRP (p¼ 0.001) levels and LVEF (p¼ 0.021) were significantly correlated with serum MGP levels. HDL-cholesterol (p¼ 0.001) and CRP (p¼ 0.030) levels were also significantly correlated with serum BMP-4 levels. In the model including HDL-cholesterol, CRP, MGP, and BMP-4, only MGP (odds ratio[OR]: 1.018, p¼ 0.019) and BMP-4 (OR: 1.313, p¼ 0.023) were found to be independently associated with ACS. Conclusion: This study shows that serum BMP-4 and MGP are independently associated with ACS occurrence, when adjusted for other CV risk factors. These biomarkers may have a diagnostic potential in ACS patients.
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