Papers by Tugba Yilmaz-Ozden
The present study was conducted to evaluate the protective effect of the water extract of the Ama... more The present study was conducted to evaluate the protective effect of the water extract of the Amaranthus lividus L., widely used as a vegetable in West Black Sea Region of Turkey, on carbon tetrachloride (CCl4)-induced oxidative stress in the kidney tissues of rats. A. lividus was collected from Bartin, Turkey. The stems with leaves and flowers of A. lividus were extracted in boiling water. The extract was filtered and evaporated to dryness under reduced pressure in a rotary evaporator. Male Wistar albino rats were pretreated with the water extract of A.lividus (250 and 500 mg/kg body weight; p.o.) once daily for 9 days and then on the 10th day CCl4 (1.5 mL/kg body weight; i.p.) in olive oil (1:1, v/v) was applied. All rats were sacrificed 24 h after CCl4 administration, kidneys were dissected out and used for biochemical studies. Antioxidant status in kidney tissues was estimated by determining the activities of the antioxidative enzymes such as catalase (CAT), superoxide dismutase...
Oxidative stress, a disbalance in favor of the prooxidants and disfavoring the antioxidants, pote... more Oxidative stress, a disbalance in favor of the prooxidants and disfavoring the antioxidants, potentially leading to cell damage [1]. Natural dietary antioxidants are extensively studied for their ability to protect cells from various damage. Amaranthus plants are widely distrubuted throughout the world and they are able to produce grains and leafy vegetables. Amaranthus lividus L. (Family:Amaranthaceae) is used as popular vegetable in West Black Sea Region of Turkey. In vitro antioxidant potential of the plant has been investigated in our earlier study [2]. The objective of the present study was to investigate the antioxidant activity of A. lividus L. against carbon tetrachloride (CCl4)-induced hepatotoxicity in rats. The stems with leaves and flowers of A. lividus were collected from Bartin, Turkey (ISTE 83401). A crude water extract was prepared by heating powdered A. lividus (10 g) with 100 mL distilled water for 30 min. The extract was filtered and evaporated to dryness under re...
Journal of Food Biochemistry, 2015
ABSTRACT The antioxidant, anti-inflammatory, acetylcholinesterase (AChE) inhibitory and antimicro... more ABSTRACT The antioxidant, anti-inflammatory, acetylcholinesterase (AChE) inhibitory and antimicrobial activities of extract from the Centaurea antiochia Boiss. var. praealta (Boiss. & Bal.) Wagenitz, an endemic plant species from Turkey, were investigated. The results indicated that the extract inhibits FeCl3/ascorbic acid-induced phosphatidylcholine liposome oxidation, scavenges stable 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) cation radicals, and reduces Fe3+ to Fe2+ in the ferric-reducing antioxidant power (FRAP) assay. The extract inhibited AChE and both cyclooxygenase (COX)-1 and COX-2, which suggests this species might be a potential source of plant-derived anti-inflammatory and anti-AChE substances. The extract may also act as an antimicrobial agent because it inhibited the growth of Gram-positive and Gram-negative bacteria as well as yeast. These findings may scientifically explain some uses of this species in Turkish folk medicine as an antimicrobial, anti-inflammatory and wound healing agent.Practical ApplicationsThe Centaurea species are known by the vernacular name “peygamber çiçeği” in Turkish folk medicine. Ethnobotanical reports indicate the Centaurea species have antidiabetic, anti-inflammatory, wound healing, digestive, stomachic, diuretic, astringent, hypotensive, antipyretic, laxative, analgesic, tonic, hemostatic and antibacterial properties.Considering the important role oxidative stress and inflammation play in the pathogenesis of neurological diseases, C. antiochia var. praealta may be used as a medicinal plant to treat Alzheimer's disease. This study also confirms the efficacy of this plant as a natural antimicrobial agent.
Bosnian journal of basic medical sciences / Udruženje basičnih mediciniskih znanosti = Association of Basic Medical Sciences, 2014
Between their broad spectrum of action, vanadium compounds are shown to have insulin mimetic/enha... more Between their broad spectrum of action, vanadium compounds are shown to have insulin mimetic/enhancing effects. Increasing evidence in experimental and clinical studies suggests that oxidative stress plays a major role in the pathogenesis of diabetes and on the onset of diabetic complications. Thus, preventive therapy can alleviate the possible side effects of the disease. The aim of the present study was to investigate the effect of vanadyl sulfate supplementation on the antioxidant system in the stomach tissue of diabetic rats. Male Swiss albino rats were randomly divided into 4 groups: control; control+vanadyl sulfate; diabetic; diabetic+vanadyl sulfate. Diabetes was induced by intraperitoneal injection of streptozotocin (STZ; 65 mg/kg body weight). Vanadyl sulfate (100 mg/kg body weight) was given daily by gavage for 60 days. At the last day of the experiment, stomach tissues were taken and homogenized to make a 10% (w/v) homogenate. Catalase (CAT), superoxide dismutase (SOD), g...
Toxicology and Industrial Health, 2014
This study was designed to evaluate the protective effect of water extract of Amaranthus lividus ... more This study was designed to evaluate the protective effect of water extract of Amaranthus lividus L. (A. lividus) (Amaranthaceae) on carbon tetrachloride (CCl4)-induced toxicity in kidneys of rats. For this purpose, male albino Wistar rats were pretreated with A. lividus (250 and 500 mg/kg body weight (b.w.)) daily for 9 days and a single dose of CCl4 was applied intraperitoneally (50% in olive oil; 1.5 mL/kg b.w.) on the 10th day. All rats were killed 24 h after CCl4 administration, and kidneys were excised and used for determination of histopathological and biochemical parameters. CCl4 administration caused a remarkable increase in lipid peroxidation (LPO) and glutathione levels and glutathione-S-transferase, glutathione peroxidase, glutathione reductase, superoxide dismutase, myeloperoxidase (MPO) activities and a decrease in catalase (CAT) activity when compared to the control group. Pretreatment with A. lividus (250 and 500 mg/kg b.w.) significantly prevented the elevation in LPO level and MPO activity as well as protected the decrease in CAT activity but did not alter other biochemical parameters. The protective effect of A. lividus was further evident through the decreased histological alterations in kidneys. In conclusion, this study has indicated that A. lividus possesses protective and antioxidant effects against CCl4-induced oxidative kidney damage.
Trace Elements and Electrolytes, 2014
Food Chemistry, 2009
Water, methanol and ethyl acetate extracts from stems with leaves and flowers of Amaranthus livid... more Water, methanol and ethyl acetate extracts from stems with leaves and flowers of Amaranthus lividus L., one of the most popular leafy vegetable consumed in the west Black Sea region of Turkey, were tested in vitro for their ability to inhibit peroxidation of phosphatidylcholine liposomes induced with Fe3+/ascorbate, to scavenge ABTS+, DPPH and hydroxyl radicals, to reduce Fe (III) to
BioMetals, 2011
In recent years, the role of free radical damage consequent to oxidative stress is widely discuss... more In recent years, the role of free radical damage consequent to oxidative stress is widely discussed in diabetic complications. In this aspect, the protection of cell integrity by trace elements is a topic to be investigated. Vanadium is a trace element believed to be important for normal cell function and development. The aim of the present study was to investigate the effect of vanadyl sulfate supplementation on the antioxidant system in the muscle tissue of diabetic rats. Diabetes was induced by intraperitoneal injection of streptozotocin (STZ, 65 mg/kg body weight) to male Swiss albino rats. The rats were randomly divided into 4 groups: Group I, control; Group II, vanadyl sulfate control; Group III, STZ-diabetic untreated; Group IV, STZ-diabetic treated with vanadyl sulfate. Vanadyl sulfate (100 mg/kg) was given daily by gavage for 60 days. At the last day of the experiment, rats were killed, muscle tissues were taken, homogenized in cold saline to make a 10% (w/v) homogenate. Body weights and blood glucose levels were estimated at 0, 30 and 60th days. Antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST), as well as carbonic anhydrase (CA), myeloperoxidase (MPO) activities and protein carbonyl content (PCC) were determined in muscle tissue. Vanadyl sulfate administration improved the loss in body weight due to STZ-induced diabetes and decreased the rise in blood glucose levels. It was shown that vanadium supplementation to diabetic rats significantly decrease serum antioxidant enzyme levels, which were significantly raised by diabetes in muscle tissue showing that this trace element could be used as preventive for diabetic complications.
In this study, the antioxidant and antiacetylcholinesterase activities of Sorbus torminalis (L.) ... more In this study, the antioxidant and antiacetylcholinesterase activities of Sorbus torminalis (L.) Crantz fruits were evaluated. Total phenolic and flavonoid compounds, 2,2-azino-bis (3-ethylbenzothioazoline-6-sulfonic acid), 2,2-diphenyl-1-picrylhydrazyl, and superoxide anion radicals scavenging activities and ferric-reducing antioxidant power of water, ethyl
acetate, acetone, and methanol extracts were determined for the measurement of the
antioxidant activity. Quercetin and a-tocopherol were used as standard antioxidants. The inhibitory effect of the water extract on acetylcholinesterase (AChE) was evaluated using the Ellman method and galantamine was used as a standard. Water extract had the highest total phenolic concentration and the strongest antioxidant activity followed by ethyl acetate and acetone extracts whereas methanol extract has the lowest phenolics and weakest antioxidant activity. Moreover, water extract showed moderate ability to inhibit AChE. It was concluded that fruits of S. torminalis have antioxidant and anti-AChE activities and that the plant might be a natural source of antioxidants and AChE inhibitors.
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Papers by Tugba Yilmaz-Ozden
acetate, acetone, and methanol extracts were determined for the measurement of the
antioxidant activity. Quercetin and a-tocopherol were used as standard antioxidants. The inhibitory effect of the water extract on acetylcholinesterase (AChE) was evaluated using the Ellman method and galantamine was used as a standard. Water extract had the highest total phenolic concentration and the strongest antioxidant activity followed by ethyl acetate and acetone extracts whereas methanol extract has the lowest phenolics and weakest antioxidant activity. Moreover, water extract showed moderate ability to inhibit AChE. It was concluded that fruits of S. torminalis have antioxidant and anti-AChE activities and that the plant might be a natural source of antioxidants and AChE inhibitors.
acetate, acetone, and methanol extracts were determined for the measurement of the
antioxidant activity. Quercetin and a-tocopherol were used as standard antioxidants. The inhibitory effect of the water extract on acetylcholinesterase (AChE) was evaluated using the Ellman method and galantamine was used as a standard. Water extract had the highest total phenolic concentration and the strongest antioxidant activity followed by ethyl acetate and acetone extracts whereas methanol extract has the lowest phenolics and weakest antioxidant activity. Moreover, water extract showed moderate ability to inhibit AChE. It was concluded that fruits of S. torminalis have antioxidant and anti-AChE activities and that the plant might be a natural source of antioxidants and AChE inhibitors.