The aims of this study were to achieve correct cultivar classification of leaf samples by using p... more The aims of this study were to achieve correct cultivar classification of leaf samples by using phyllometric parameters to determine the performance of parameters applied as the discriminant criteria and to determine the minimal number of leaf parameters needed to accurately classify leaf samples within cultivars. Seventy-nine phyllometric parameters were measured/calculated on 360 leaf samples from eleven grapevine cultivars, gathered during several years, from different growing conditions and with some differences in the sampling methods applied. Stepwise discriminant analysis was used to rank the phyllometric parameters according to their efficiency in the discrimination of cultivars. A series of discriminant analyses was performed with successive introduction of new parameters as discriminatory elements until 100 % correct classification of leaf samples into the correct cultivar was achieved. This was achieved using only the seven highest ranking phyllometric parameters from the...
Non-targeted metabolomics research of human volatile urinary metabolome can be used to identify p... more Non-targeted metabolomics research of human volatile urinary metabolome can be used to identify potential biomarkers associated with the changes in metabolism related to various health disorders. To ensure reliable analysis of urinary volatile organic metabolites (VOMs) by gas chromatography-mass spectrometry (GC-MS), parameters affecting the headspace-solid phase microextraction (HS-SPME) procedure have been evaluated and optimized. The influence of incubation and extraction temperatures and times, coating fibre material and salt addition on SPME efficiency was investigated by multivariate optimization methods using reduced factorial and Doehlert matrix designs. The results showed optimum values for temperature to be 60°C, extraction time 50min, and incubation time 35min. The proposed conditions were applied to investigate urine samples' stability regarding different storage conditions and freeze-thaw processes. The sum of peak areas of urine samples stored at 4°C, -20°C, and -...
Association Abieti-Fagetum "pannonicum" Raus 1969 has an invalid name and still undefin... more Association Abieti-Fagetum "pannonicum" Raus 1969 has an invalid name and still undefined position in geobotanical and phytosociological sense. In 2000 and 2001, within the Abieti-Fagetum ass. on central part of Medvednica mountain, the flora and different habitat factors of 12 plots (50 x 50 m) were investigated. On the whole, 119 taxa of vascular plants were recorded (one subendemic and three vulnerable). In spite of being considered a differential species for Dinaric fir-beech forests, the species Sorbus aucuparia L. was recorded with a high percent of share. The life form analysis confirmed that Medvednica’ s flora belongs to the moderate continental climate. The influence of habitat factors on floristic variability of the plots was estimated by Canonical Correspondence Analysis. The changes in altitude, pH and inclination have the strongest influence on the changes of floristic structure.
Sympatric species with similar niches compete for common resources, often resulting in spatio-tem... more Sympatric species with similar niches compete for common resources, often resulting in spatio-temporal avoidance. To date, little is known about the spatio-temporal interactions of ungulates in forested habitats. Using camera traps, we investigated the spatio-temporal responses of alpine chamois to the presence of red deer in the mountain forests of northern Croatia during the four seasons of 2018/2019. We hypothesized that chamois activity patterns should be affected by the presence of red deer, leading to either temporal or spatial segregation between the species. Our results showed that chamois tended to be more active during the day when red deer were absent, and showed contrasting patterns of activity across seasons, from bimodal to multimodal. However, temporal overlap coefficients between chamois and red deer were moderate throughout the study period, ranging from 0.57 in summer to 0.68 in autumn. Conversely, we found inconsistent/random occupancy patterns of chamois and red deer at different camera trap locations in different seasons. Our results are among the few available data on the interaction of these two species in forested areas and suggest weak effects of red deer on the spatio-temporal behaviour of Alpine chamois. However, finer-scale data may be required to better understand the relationship between these species.
This study evaluates different techniques available for identifying clusters and boundary detecti... more This study evaluates different techniques available for identifying clusters and boundary detection within populations. It nicely demonstrates the potential of Bayesian spatial clustering algorithms."-Dr. Guido R. M. M. Haenen "While somewhat specialized, this study can have valuable practical utility in understanding where geographic edges lie between subspecies.
Simple Summary Two species of chamois (Rupicapra rupicapra and R. pyrenaica) are currently recogn... more Simple Summary Two species of chamois (Rupicapra rupicapra and R. pyrenaica) are currently recognized by taxonomy and further subdivided into seven and three subspecies, respectively. However, recent research based on molecular markers finds this classification questionable. We aim to increase the resolution of published research on chamois phylogeny by including mitogenomes of all available subspecies, including the previously unpublished mitogenomes of R. r. balcanica and R. r. tatrica subspecies. The inferred phylogeny based on the full mitogenomes confirms the previously reported genus subdivision in three clades and its monophyletic positioning within the Caprinae. Phylogeny and taxonomy of Rupicapra species thus remain controversial prompting for the inclusion of archeological remains to solve the controversy. Abstract Although the two species of chamois (Rupicapra rupicapra and R. pyrenaica) are currently classified as least-concern by the IUCN (International Union for Conser...
ABSTRACT Sazetak Kako bi se utvrdio nastanak i razvoj populacije dubrovacke ovce u Republic! Hrva... more ABSTRACT Sazetak Kako bi se utvrdio nastanak i razvoj populacije dubrovacke ovce u Republic! Hrvatskoj tipizirani su lokusi mikrosatelitne DNA. Ocijenjene su alelne varijante, stupanj heterozigotnosti i stupanj polimorfnosti pojedinih markera. Tipizirano je 10 mikrosatelitnih lokusa na 44 jedinke dubrovacke ovce. ProsjeCna vrijednost alela po mikrosatelitnom lokusu iznosi 4,60, a srednja vrijednosti oCekivane heterozigotnosti He=0,6575. Najveci broj uocenih alela tipiziranih lokusa iznosi osam, a prisutan je na lokusu OarAEl 19. Prosjecna PIC vrijednost potvrduje visoku polimorfnost istrazivanih mikrosatelitnih markera (PIC > 0,50). Srednja F|s vrijednost istrazivanih lokusa u populaciji dubrovacke ovce je pozitivna (Fjs = 0,094) Sto upucuje na postojanje inbridinga sto je i logiCno s obzirom na velicinu ukupne populacije i na razmjenu ovnova izmedu pojedinih stada. Kljucne rijeci: alelna varijanta, dubrovacka ovca, geneticka struktura, mikrosatelitni lokus Uvod Ocuvanje prirodnog stani §ta i genetskog polimorfizma vrlo je vazno za prezivljavanja mnogih ugrozenih vrsta, kao i onih vrsta cije je prirodno staniste drasticno smanjeno. Stogaje, narocito zaugrozene populacije, potrebno ocuvati sto veci stupanj heterozigotnosti u populaciji, jer je tada veci i postotak prezivljavanja. Medutim, cesto je situacija suprotna, te ugrozene populacije imaju vrlo nizak stupanj heterozigotnosti, sto je najcesca posljedica efekta «uskog grla» (Carvalho, 1993). Hrvatska, zbog posebnog zemljopisnog polozaja posjeduje bogatu bioloSku raznolikost. Intenzifikacija poljoprivredne proizvodnje prouzrocila je zamjenu nisko produktivnih autohtonih pasmina s visoko produktivnim, selekcioniranim pasminama. Posljednja dva desetljeca Hrvatska se u skladu sa svjetskim trendovima pocela baviti upoznavanjem i zastitom autohtonih pasmina domacih zivotinja. Uspjesno je zapoCelo uredenje maticnih knjiga autohtonih pasmina kao i prvih programa oCuvanja i zastite. Prije negoli bi se mogao implementirati program oCuvanja geneticke razlicitosti potrebno ga je objektivno ispitati.
The aim of this study was to evaluate the effect of a supplementation diet for hens consisting of... more The aim of this study was to evaluate the effect of a supplementation diet for hens consisting of dried basil herb and flowers of calendula and dandelion for color, carotenoid content, iron-induced oxidative stability, and sensory properties of egg yolk compared with commercial pigment (control) and marigold flower. The plant parts were supplemented in diets at two levels: 1% and 3%. In response to dietary content, yolks from all diets differed in carotenoid profile (p < 0.001). The 3% supplementation level resulted in a similar total carotenoid content as the control (21.25 vs. 21.79 μg/g), but by 3-fold lower compared to the 3% marigold (66.95 μg/g). The tested plants did not achieve yolk color fan values as the control (13.47) or 3% marigold (11.47), and among them, calendula had the highest values (9.73). Despite the low carotenoid content in diets supplemented with basil herb, iron-induced malondialdehyde (MDA) concentration was low as for marigold (on average 106.83 vs. 92....
There is increasing recognition of the occurrence of non-native species that are invasive and pot... more There is increasing recognition of the occurrence of non-native species that are invasive and potentially contribute to biodiversity loss. A two-year camera trap survey was undertaken on Mountain Mosor, Croatia to determine the daily and seasonal activity patterns of recently introduced non-native aoudad (Ammotragus lervia). Aoudad were most active in open rocky habitats and least active in forest habitats. The effect of habitat on the recorded number of aoudad was significant, while the effects of month and the interaction month × habitat were not. The results showed a typical bimodal activity pattern of aoudad, with a modest peak in activity between 5:00 and 9:00 a.m., and a second, more pronounced activity peak between 5:00 and 7:00 p.m. Since the native habitat of aoudad is similar to that in the Mediterranean region, the inferred range of daily and seasonal activities show that the species is well adapted to the new habitat.
The present study was undertaken to evaluate efficiency of callogenesis and regeneration by somat... more The present study was undertaken to evaluate efficiency of callogenesis and regeneration by somatic embryogenesis of the subendemic Iris species Iris illyrica from Croatia and to select highly regenerative donor plants/genotypes. Leaf base segments were used as explants. Callogenesis and somatic embryogenesis were induced on MS media supplemented with: (1) 4.52 M 2,4-dichlorophenoxyacetic acid (2,4-D) + 4.83 M 1-naphthaleneacetic acid (NAA) + 0.46 M kinetin (Kin); (2) 4.52 M 2,4-D + 4.6 M Kin; (3) 13.4 M NAA + 2.3 M Kin. Transfer of embryogenic calli onto hormone-free medium enabled the development of mature somatic embryos. Frequency of callogenesis was influenced by the donor plant. Among 15 donor plants tested, 3 of them exhibited high regeneration capability and produced 87 regenerants. Seven morphological traits were observed in order to assess phenotypic variability of flowering regenerants. Regenerants with higher values of fall and standard width and length show potential for further breeding of new varieties of Illyrian iris. Flowering and non-flowering regenerants had the same ploidy level as donor plants. Also, the nuclear DNA content (2C = 12.936 ± 0.038 pg) of this species was estimated for the first time using flow cytometry.
The present study was undertaken to evaluate efficiency of callogenesis and regeneration by somat... more The present study was undertaken to evaluate efficiency of callogenesis and regeneration by somatic embryogenesis of the subendemic Iris species Iris illyrica from Croatia and to select highly regenerative donor plants/genotypes. Leaf base segments were used as explants. Callogenesis and somatic embryogenesis were induced on MS media supplemented with: (1) 4.52 M 2,4-dichlorophenoxyacetic acid (2,4-D) + 4.83 M 1-naphthaleneacetic acid (NAA) + 0.46 M kinetin (Kin); (2) 4.52 M 2,4-D + 4.6 M Kin; (3) 13.4 M NAA + 2.3 M Kin. Transfer of embryogenic calli onto hormone-free medium enabled the development of mature somatic embryos. Frequency of callogenesis was influenced by the donor plant. Among 15 donor plants tested, 3 of them exhibited high regeneration capability and produced 87 regenerants. Seven morphological traits were observed in order to assess phenotypic variability of flowering regenerants. Regenerants with higher values of fall and standard width and length show potential for further breeding of new varieties of Illyrian iris. Flowering and non-flowering regenerants had the same ploidy level as donor plants. Also, the nuclear DNA content (2C = 12.936 ± 0.038 pg) of this species was estimated for the first time using flow cytometry.
Soil bulk density (BD) is often used to assess soil properties related to soil structure and gene... more Soil bulk density (BD) is often used to assess soil properties related to soil structure and general soil quality, or to convert weight units to volume/area units. Because collecting undisturbed soil samples (cores) and directly measuring BD is laborious and time-consuming, data on BD are often lacking. Pedotransfer functions (PTFs) can be used to predict BD from more readily available (easily measured) properties. However, specific PTFs for specific range of soils should be developed. In this study, soil organic matter (SOM) and clay contents of 90 forest Pseudogleys horizons, distributed across 11 locations in continental Croatia, were used to develop a multiple linear regression equation that predicts BD. The results have shown that the increase in BD due to the unit increase in clay content is lower than the decrease in BD due to the unit decrease in SOM content. PTF performance was relatively high (R 2 adj=77.5%, RMSE=0.1 g*cm-3 , MAE=0.08 g*cm-3), showing that missing data on soil BD of Croatian Pseudogleys (or other similar soils, formed on similar parent materials) can be estimated reasonably well by using it. However, care should be taken when dealing with arable soils, which have different properties, compared with the natural soils considered herein.
This is an open access article under the terms of the Creative Commons Attribution License, which... more This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
The aims of this study were to achieve correct cultivar classification of leaf samples by using p... more The aims of this study were to achieve correct cultivar classification of leaf samples by using phyllometric parameters to determine the performance of parameters applied as the discriminant criteria and to determine the minimal number of leaf parameters needed to accurately classify leaf samples within cultivars. Seventy-nine phyllometric parameters were measured/calculated on 360 leaf samples from eleven grapevine cultivars, gathered during several years, from different growing conditions and with some differences in the sampling methods applied. Stepwise discriminant analysis was used to rank the phyllometric parameters according to their efficiency in the discrimination of cultivars. A series of discriminant analyses was performed with successive introduction of new parameters as discriminatory elements until 100 % correct classification of leaf samples into the correct cultivar was achieved. This was achieved using only the seven highest ranking phyllometric parameters from the...
Non-targeted metabolomics research of human volatile urinary metabolome can be used to identify p... more Non-targeted metabolomics research of human volatile urinary metabolome can be used to identify potential biomarkers associated with the changes in metabolism related to various health disorders. To ensure reliable analysis of urinary volatile organic metabolites (VOMs) by gas chromatography-mass spectrometry (GC-MS), parameters affecting the headspace-solid phase microextraction (HS-SPME) procedure have been evaluated and optimized. The influence of incubation and extraction temperatures and times, coating fibre material and salt addition on SPME efficiency was investigated by multivariate optimization methods using reduced factorial and Doehlert matrix designs. The results showed optimum values for temperature to be 60°C, extraction time 50min, and incubation time 35min. The proposed conditions were applied to investigate urine samples' stability regarding different storage conditions and freeze-thaw processes. The sum of peak areas of urine samples stored at 4°C, -20°C, and -...
Association Abieti-Fagetum "pannonicum" Raus 1969 has an invalid name and still undefin... more Association Abieti-Fagetum "pannonicum" Raus 1969 has an invalid name and still undefined position in geobotanical and phytosociological sense. In 2000 and 2001, within the Abieti-Fagetum ass. on central part of Medvednica mountain, the flora and different habitat factors of 12 plots (50 x 50 m) were investigated. On the whole, 119 taxa of vascular plants were recorded (one subendemic and three vulnerable). In spite of being considered a differential species for Dinaric fir-beech forests, the species Sorbus aucuparia L. was recorded with a high percent of share. The life form analysis confirmed that Medvednica’ s flora belongs to the moderate continental climate. The influence of habitat factors on floristic variability of the plots was estimated by Canonical Correspondence Analysis. The changes in altitude, pH and inclination have the strongest influence on the changes of floristic structure.
Sympatric species with similar niches compete for common resources, often resulting in spatio-tem... more Sympatric species with similar niches compete for common resources, often resulting in spatio-temporal avoidance. To date, little is known about the spatio-temporal interactions of ungulates in forested habitats. Using camera traps, we investigated the spatio-temporal responses of alpine chamois to the presence of red deer in the mountain forests of northern Croatia during the four seasons of 2018/2019. We hypothesized that chamois activity patterns should be affected by the presence of red deer, leading to either temporal or spatial segregation between the species. Our results showed that chamois tended to be more active during the day when red deer were absent, and showed contrasting patterns of activity across seasons, from bimodal to multimodal. However, temporal overlap coefficients between chamois and red deer were moderate throughout the study period, ranging from 0.57 in summer to 0.68 in autumn. Conversely, we found inconsistent/random occupancy patterns of chamois and red deer at different camera trap locations in different seasons. Our results are among the few available data on the interaction of these two species in forested areas and suggest weak effects of red deer on the spatio-temporal behaviour of Alpine chamois. However, finer-scale data may be required to better understand the relationship between these species.
This study evaluates different techniques available for identifying clusters and boundary detecti... more This study evaluates different techniques available for identifying clusters and boundary detection within populations. It nicely demonstrates the potential of Bayesian spatial clustering algorithms."-Dr. Guido R. M. M. Haenen "While somewhat specialized, this study can have valuable practical utility in understanding where geographic edges lie between subspecies.
Simple Summary Two species of chamois (Rupicapra rupicapra and R. pyrenaica) are currently recogn... more Simple Summary Two species of chamois (Rupicapra rupicapra and R. pyrenaica) are currently recognized by taxonomy and further subdivided into seven and three subspecies, respectively. However, recent research based on molecular markers finds this classification questionable. We aim to increase the resolution of published research on chamois phylogeny by including mitogenomes of all available subspecies, including the previously unpublished mitogenomes of R. r. balcanica and R. r. tatrica subspecies. The inferred phylogeny based on the full mitogenomes confirms the previously reported genus subdivision in three clades and its monophyletic positioning within the Caprinae. Phylogeny and taxonomy of Rupicapra species thus remain controversial prompting for the inclusion of archeological remains to solve the controversy. Abstract Although the two species of chamois (Rupicapra rupicapra and R. pyrenaica) are currently classified as least-concern by the IUCN (International Union for Conser...
ABSTRACT Sazetak Kako bi se utvrdio nastanak i razvoj populacije dubrovacke ovce u Republic! Hrva... more ABSTRACT Sazetak Kako bi se utvrdio nastanak i razvoj populacije dubrovacke ovce u Republic! Hrvatskoj tipizirani su lokusi mikrosatelitne DNA. Ocijenjene su alelne varijante, stupanj heterozigotnosti i stupanj polimorfnosti pojedinih markera. Tipizirano je 10 mikrosatelitnih lokusa na 44 jedinke dubrovacke ovce. ProsjeCna vrijednost alela po mikrosatelitnom lokusu iznosi 4,60, a srednja vrijednosti oCekivane heterozigotnosti He=0,6575. Najveci broj uocenih alela tipiziranih lokusa iznosi osam, a prisutan je na lokusu OarAEl 19. Prosjecna PIC vrijednost potvrduje visoku polimorfnost istrazivanih mikrosatelitnih markera (PIC &gt; 0,50). Srednja F|s vrijednost istrazivanih lokusa u populaciji dubrovacke ovce je pozitivna (Fjs = 0,094) Sto upucuje na postojanje inbridinga sto je i logiCno s obzirom na velicinu ukupne populacije i na razmjenu ovnova izmedu pojedinih stada. Kljucne rijeci: alelna varijanta, dubrovacka ovca, geneticka struktura, mikrosatelitni lokus Uvod Ocuvanje prirodnog stani §ta i genetskog polimorfizma vrlo je vazno za prezivljavanja mnogih ugrozenih vrsta, kao i onih vrsta cije je prirodno staniste drasticno smanjeno. Stogaje, narocito zaugrozene populacije, potrebno ocuvati sto veci stupanj heterozigotnosti u populaciji, jer je tada veci i postotak prezivljavanja. Medutim, cesto je situacija suprotna, te ugrozene populacije imaju vrlo nizak stupanj heterozigotnosti, sto je najcesca posljedica efekta «uskog grla» (Carvalho, 1993). Hrvatska, zbog posebnog zemljopisnog polozaja posjeduje bogatu bioloSku raznolikost. Intenzifikacija poljoprivredne proizvodnje prouzrocila je zamjenu nisko produktivnih autohtonih pasmina s visoko produktivnim, selekcioniranim pasminama. Posljednja dva desetljeca Hrvatska se u skladu sa svjetskim trendovima pocela baviti upoznavanjem i zastitom autohtonih pasmina domacih zivotinja. Uspjesno je zapoCelo uredenje maticnih knjiga autohtonih pasmina kao i prvih programa oCuvanja i zastite. Prije negoli bi se mogao implementirati program oCuvanja geneticke razlicitosti potrebno ga je objektivno ispitati.
The aim of this study was to evaluate the effect of a supplementation diet for hens consisting of... more The aim of this study was to evaluate the effect of a supplementation diet for hens consisting of dried basil herb and flowers of calendula and dandelion for color, carotenoid content, iron-induced oxidative stability, and sensory properties of egg yolk compared with commercial pigment (control) and marigold flower. The plant parts were supplemented in diets at two levels: 1% and 3%. In response to dietary content, yolks from all diets differed in carotenoid profile (p < 0.001). The 3% supplementation level resulted in a similar total carotenoid content as the control (21.25 vs. 21.79 μg/g), but by 3-fold lower compared to the 3% marigold (66.95 μg/g). The tested plants did not achieve yolk color fan values as the control (13.47) or 3% marigold (11.47), and among them, calendula had the highest values (9.73). Despite the low carotenoid content in diets supplemented with basil herb, iron-induced malondialdehyde (MDA) concentration was low as for marigold (on average 106.83 vs. 92....
There is increasing recognition of the occurrence of non-native species that are invasive and pot... more There is increasing recognition of the occurrence of non-native species that are invasive and potentially contribute to biodiversity loss. A two-year camera trap survey was undertaken on Mountain Mosor, Croatia to determine the daily and seasonal activity patterns of recently introduced non-native aoudad (Ammotragus lervia). Aoudad were most active in open rocky habitats and least active in forest habitats. The effect of habitat on the recorded number of aoudad was significant, while the effects of month and the interaction month × habitat were not. The results showed a typical bimodal activity pattern of aoudad, with a modest peak in activity between 5:00 and 9:00 a.m., and a second, more pronounced activity peak between 5:00 and 7:00 p.m. Since the native habitat of aoudad is similar to that in the Mediterranean region, the inferred range of daily and seasonal activities show that the species is well adapted to the new habitat.
The present study was undertaken to evaluate efficiency of callogenesis and regeneration by somat... more The present study was undertaken to evaluate efficiency of callogenesis and regeneration by somatic embryogenesis of the subendemic Iris species Iris illyrica from Croatia and to select highly regenerative donor plants/genotypes. Leaf base segments were used as explants. Callogenesis and somatic embryogenesis were induced on MS media supplemented with: (1) 4.52 M 2,4-dichlorophenoxyacetic acid (2,4-D) + 4.83 M 1-naphthaleneacetic acid (NAA) + 0.46 M kinetin (Kin); (2) 4.52 M 2,4-D + 4.6 M Kin; (3) 13.4 M NAA + 2.3 M Kin. Transfer of embryogenic calli onto hormone-free medium enabled the development of mature somatic embryos. Frequency of callogenesis was influenced by the donor plant. Among 15 donor plants tested, 3 of them exhibited high regeneration capability and produced 87 regenerants. Seven morphological traits were observed in order to assess phenotypic variability of flowering regenerants. Regenerants with higher values of fall and standard width and length show potential for further breeding of new varieties of Illyrian iris. Flowering and non-flowering regenerants had the same ploidy level as donor plants. Also, the nuclear DNA content (2C = 12.936 ± 0.038 pg) of this species was estimated for the first time using flow cytometry.
The present study was undertaken to evaluate efficiency of callogenesis and regeneration by somat... more The present study was undertaken to evaluate efficiency of callogenesis and regeneration by somatic embryogenesis of the subendemic Iris species Iris illyrica from Croatia and to select highly regenerative donor plants/genotypes. Leaf base segments were used as explants. Callogenesis and somatic embryogenesis were induced on MS media supplemented with: (1) 4.52 M 2,4-dichlorophenoxyacetic acid (2,4-D) + 4.83 M 1-naphthaleneacetic acid (NAA) + 0.46 M kinetin (Kin); (2) 4.52 M 2,4-D + 4.6 M Kin; (3) 13.4 M NAA + 2.3 M Kin. Transfer of embryogenic calli onto hormone-free medium enabled the development of mature somatic embryos. Frequency of callogenesis was influenced by the donor plant. Among 15 donor plants tested, 3 of them exhibited high regeneration capability and produced 87 regenerants. Seven morphological traits were observed in order to assess phenotypic variability of flowering regenerants. Regenerants with higher values of fall and standard width and length show potential for further breeding of new varieties of Illyrian iris. Flowering and non-flowering regenerants had the same ploidy level as donor plants. Also, the nuclear DNA content (2C = 12.936 ± 0.038 pg) of this species was estimated for the first time using flow cytometry.
Soil bulk density (BD) is often used to assess soil properties related to soil structure and gene... more Soil bulk density (BD) is often used to assess soil properties related to soil structure and general soil quality, or to convert weight units to volume/area units. Because collecting undisturbed soil samples (cores) and directly measuring BD is laborious and time-consuming, data on BD are often lacking. Pedotransfer functions (PTFs) can be used to predict BD from more readily available (easily measured) properties. However, specific PTFs for specific range of soils should be developed. In this study, soil organic matter (SOM) and clay contents of 90 forest Pseudogleys horizons, distributed across 11 locations in continental Croatia, were used to develop a multiple linear regression equation that predicts BD. The results have shown that the increase in BD due to the unit increase in clay content is lower than the decrease in BD due to the unit decrease in SOM content. PTF performance was relatively high (R 2 adj=77.5%, RMSE=0.1 g*cm-3 , MAE=0.08 g*cm-3), showing that missing data on soil BD of Croatian Pseudogleys (or other similar soils, formed on similar parent materials) can be estimated reasonably well by using it. However, care should be taken when dealing with arable soils, which have different properties, compared with the natural soils considered herein.
This is an open access article under the terms of the Creative Commons Attribution License, which... more This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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