Skip to main content: My Account; Help; About; Home. ePublications@SCU. ePublications@SCU. < P... more Skip to main content: My Account; Help; About; Home. ePublications@SCU. ePublications@SCU. < Previous; Next >; Home > Plant Science papers > 406. Southern Cross Plant Science. Title. Detection of SNPs in ESTs from wheat and barley. Authors. Peter C. Bundock, Southern Cross UniversityFollow Timothy A. Holton John T. Christopher, Southern Cross University Robert J. Henry, Southern Cross UniversityFollow. Custom Citation (Optional). Bundock ...
Abstract The Australian Agriculture Research Institute is embarking on a large-scale grape EST an... more Abstract The Australian Agriculture Research Institute is embarking on a large-scale grape EST analysis project. cDNA libraries are being prepared from a range of tissues, developmental stages and cultivars with the aim of producing a data base of up to 50,000 sequences, covering a high proportion of grape genes. 2,466 sequences have been obtained from a leaf cDNA library, and 2,438 from a berry library. Analysis of the leaf and berry EST's indicate 59% of clones have significant homology with known plant genes, 16 ...
Skip to main content: My Account; Help; About; Home. ePublications@SCU. ePublications@SCU. < P... more Skip to main content: My Account; Help; About; Home. ePublications@SCU. ePublications@SCU. < Previous; Next >; Home > Plant Science papers > 471. Southern Cross Plant Science. Title. DNA based cereal variety identification. Authors. Timothy A. Holton Robert J. Henry, Southern Cross UniversityFollow H S. Saini Mervyn Shepherd, Southern Cross UniversityFollow. Custom Citation (Optional). Holton, TA, Henry, RJ, Saini, HS & Shepherd, M 1999 ...
... Ferdinand / Henney, Adriano / Hirata, Rosario DC / Ingelman-Sundberg, Magnus / Llerena, Adria... more ... Ferdinand / Henney, Adriano / Hirata, Rosario DC / Ingelman-Sundberg, Magnus / Llerena, Adrian / Manolopoulos, Vangelis G. / Meyer, Urs A. / Patrinos, George / Seeringer, Angela / Schaik, Ron ... Hfl acts in the corolla, stigma and pollen, whilst Hf2 only acts in the corolla limb. ...
'Classical' flower breeding by continuous crossing and selection has its limitations; f... more 'Classical' flower breeding by continuous crossing and selection has its limitations; for example, no one has succeeded in breeding a blue rose or an orange petunia. However, the ability to introduce individual genes into plants (molecular breeding) has made the ...
The regions of the spinach and pea chloroplast genomes containing the ATP synthase genes atpA, at... more The regions of the spinach and pea chloroplast genomes containing the ATP synthase genes atpA, atpF and atpH have been sequenced. The encoded proteins, CF1 alpha, CF0I and CF0III, are well conserved between spinach and pea, and analogous to the alpha, b and c subunits of the Escherichia coli ATP synthase complex. The atpF gene is split by a single intron, and the exon/intron boundaries have been defined by isolating and sequencing a partial cDNA clone. Two other genes, designated atpI and rps2, located upstream from atpH, have also been sequenced. They encode a 27,000 Mr hydrophobic protein analogous to the F0a subunit of E. coli ATP synthase and a basic protein analogous to the S2 protein of the E. coli 30 S ribosomal subunit. Transcriptional analysis by electron microscopy of RNA-DNA hybrids, Northern blotting and primer extension experiments shows that these genes are transcribed and processed into a complex set of transcripts, with 5&#39; ends mapping upstream from the rps2, atpI and atpH genes.
The genes encoding the structural components of nitrogenase, niffrl, n#D and nifX, from the fast-... more The genes encoding the structural components of nitrogenase, niffrl, n#D and nifX, from the fast-growing, broad-host-range ~hi~obium strain ANU240 have been identified and characterized. They are duplicated and linked in an operon nifHDK in both copies. Sequence analysis of the nifH gene from each copy, together with partial sequence analysis of the nz$D and nzjiY genes, and restriction endonuclease analysis suggested that the duplication is precise. Comparison of the Fe-protein sequence from strain ANU240 with that from other nitrogen-fixing organisms revealed that, despite its broad host range and certain physiological properties characteristic of Bradyrhizobium strains, ANU240 is more closely related to the narrow-host-range Rhizobium strains than to the broad-host-range Brudyrhizobium strains. The promoter regions of both copies of the nif genes contain the consensus sequence characteristic of nif promoters, and functional analysis of the two promoters suggested that both nif operons are transcribed in noduIes.
The most widely used molecular marker system is based on the use of RFLPs. These are highly relia... more The most widely used molecular marker system is based on the use of RFLPs. These are highly reliable and informative but they are technically demanding to assay and expensive. Marker assays based on the Polymerase Chain Reaction (PCR) offer a far more user-friendly marker system. PCR based techniques such as RAPDs are unreliable and generally not transferable between crosses. However, microsatellites (di- or tri-nucleotide repeat sequences) also known as Simple Sequence Repeats (SSR) offer highly reliable, co-dominant markers. Microsatellites are becoming more widely used for marker assisted breeding and variety identification due to their high level of polymorphism and ease of use. Forty-three barley microsatellite markers were used to genotype 180 barley varieties. Primer pairs were synthesized and screened on a panel of four genetically diverse genotypes. Seventeen of them giving unambiguous and polymorphic DNA banding patterns were selected for allele typing study. Allele typing...
Harker, N, Hayden, M, Henry, RJ, Holton, TA, Karakousis, A, Langridge, P, McLauchlin, A, Morell, ... more Harker, N, Hayden, M, Henry, RJ, Holton, TA, Karakousis, A, Langridge, P, McLauchlin, A, Morell, MK, Preston, L, Shariflou, MR &amp; Sharp, PJ 1999, &#x27;Microsatellite analysis of Australian wheat varieties&#x27;, in JF Panozzo, M Ratcliffe, M Wooton &amp; CW Wrigley (eds), Cereals 99: Proceedings of the 49th Australian Cereal Chemistry Conference, Melbourne, Vic., 23-16 September, Cereal Chemistry Division, Royal Australian Chemical Institute, North Melbourne, Vic.
A full length cDNA clone encoding rose dihydroflavonol 4-reductase (DFR) was isolated from a cDNA... more A full length cDNA clone encoding rose dihydroflavonol 4-reductase (DFR) was isolated from a cDNA library derived from rose petals by screening with the cDNA of Petunia hybrida DFR. Sequence comparison of the rose DFR with reported DFR genes revealed that they are homologous to each other. The amount of DFR mRNA in rose petals was developmentally regulated and paralleled anthocyanin production in petals. Sepals, thorns, styles and stamens also contained anthocyanins and DFR mRNA. No DFR mRNA was observed in mature leaves and a small amount of the transcript was detected in young leaves. A petunia cultivar, whose colour was pale pink due to a deficiency in flavonoid 3'-hydroxylase and flavonoid 3',5'-hydroxylase, was transformed with a binary vector containing a rose DFR cDNA cloned behind a constitutive promoter. Petals and anthers of the resultant transgenic petunia plants were salmon pink and contained pelargonidin, an anthocyanidin rarely found in petunia.
... Flavonoid biosynthesis genes from grape (Vitis vinifera) isolated by EST analysis. Daniel LE ... more ... Flavonoid biosynthesis genes from grape (Vitis vinifera) isolated by EST analysis. Daniel LE Waters, Southern Cross University Timothy A. Holton Effie M. Ablett, Southern Cross University L Slade Lee, Southern Cross University Robert J. Henry, Southern Cross University. ...
The quality of tropical grasses is a major limitation to animal production in tropical and subtro... more The quality of tropical grasses is a major limitation to animal production in tropical and subtropical areas. This is mainly associated with the lower digestibility because C4 grasses have higher fibre levels. Any improvement in quality would require a reduction in the lignin and an increase in the digestion of the neutral detergent fibre content of these plants (Clark and Wilson 1993). Kikuyu (Pennisetum clandestinum) is an important grass for the dairy and beef industries of the subtropics of Australia, South Africa and New Zealand (Mears 1970). Increased digestibility could substantially improve animal production in these industries. These experiments investigated the variation in agronomic and quality of natural populations selected from diverse regions within Australia. Runners of 14 kikuyu selections were collected by project staff or local agronomists from areas considered to have grown kikuyu for over 30 years while Whittet and Noonan were established by seed. Entries were e...
Skip to main content: My Account; Help; About; Home. ePublications@SCU. ePublications@SCU. < P... more Skip to main content: My Account; Help; About; Home. ePublications@SCU. ePublications@SCU. < Previous; Next >; Home > Plant Science papers > 406. Southern Cross Plant Science. Title. Detection of SNPs in ESTs from wheat and barley. Authors. Peter C. Bundock, Southern Cross UniversityFollow Timothy A. Holton John T. Christopher, Southern Cross University Robert J. Henry, Southern Cross UniversityFollow. Custom Citation (Optional). Bundock ...
Abstract The Australian Agriculture Research Institute is embarking on a large-scale grape EST an... more Abstract The Australian Agriculture Research Institute is embarking on a large-scale grape EST analysis project. cDNA libraries are being prepared from a range of tissues, developmental stages and cultivars with the aim of producing a data base of up to 50,000 sequences, covering a high proportion of grape genes. 2,466 sequences have been obtained from a leaf cDNA library, and 2,438 from a berry library. Analysis of the leaf and berry EST's indicate 59% of clones have significant homology with known plant genes, 16 ...
Skip to main content: My Account; Help; About; Home. ePublications@SCU. ePublications@SCU. < P... more Skip to main content: My Account; Help; About; Home. ePublications@SCU. ePublications@SCU. < Previous; Next >; Home > Plant Science papers > 471. Southern Cross Plant Science. Title. DNA based cereal variety identification. Authors. Timothy A. Holton Robert J. Henry, Southern Cross UniversityFollow H S. Saini Mervyn Shepherd, Southern Cross UniversityFollow. Custom Citation (Optional). Holton, TA, Henry, RJ, Saini, HS & Shepherd, M 1999 ...
... Ferdinand / Henney, Adriano / Hirata, Rosario DC / Ingelman-Sundberg, Magnus / Llerena, Adria... more ... Ferdinand / Henney, Adriano / Hirata, Rosario DC / Ingelman-Sundberg, Magnus / Llerena, Adrian / Manolopoulos, Vangelis G. / Meyer, Urs A. / Patrinos, George / Seeringer, Angela / Schaik, Ron ... Hfl acts in the corolla, stigma and pollen, whilst Hf2 only acts in the corolla limb. ...
'Classical' flower breeding by continuous crossing and selection has its limitations; f... more 'Classical' flower breeding by continuous crossing and selection has its limitations; for example, no one has succeeded in breeding a blue rose or an orange petunia. However, the ability to introduce individual genes into plants (molecular breeding) has made the ...
The regions of the spinach and pea chloroplast genomes containing the ATP synthase genes atpA, at... more The regions of the spinach and pea chloroplast genomes containing the ATP synthase genes atpA, atpF and atpH have been sequenced. The encoded proteins, CF1 alpha, CF0I and CF0III, are well conserved between spinach and pea, and analogous to the alpha, b and c subunits of the Escherichia coli ATP synthase complex. The atpF gene is split by a single intron, and the exon/intron boundaries have been defined by isolating and sequencing a partial cDNA clone. Two other genes, designated atpI and rps2, located upstream from atpH, have also been sequenced. They encode a 27,000 Mr hydrophobic protein analogous to the F0a subunit of E. coli ATP synthase and a basic protein analogous to the S2 protein of the E. coli 30 S ribosomal subunit. Transcriptional analysis by electron microscopy of RNA-DNA hybrids, Northern blotting and primer extension experiments shows that these genes are transcribed and processed into a complex set of transcripts, with 5&#39; ends mapping upstream from the rps2, atpI and atpH genes.
The genes encoding the structural components of nitrogenase, niffrl, n#D and nifX, from the fast-... more The genes encoding the structural components of nitrogenase, niffrl, n#D and nifX, from the fast-growing, broad-host-range ~hi~obium strain ANU240 have been identified and characterized. They are duplicated and linked in an operon nifHDK in both copies. Sequence analysis of the nifH gene from each copy, together with partial sequence analysis of the nz$D and nzjiY genes, and restriction endonuclease analysis suggested that the duplication is precise. Comparison of the Fe-protein sequence from strain ANU240 with that from other nitrogen-fixing organisms revealed that, despite its broad host range and certain physiological properties characteristic of Bradyrhizobium strains, ANU240 is more closely related to the narrow-host-range Rhizobium strains than to the broad-host-range Brudyrhizobium strains. The promoter regions of both copies of the nif genes contain the consensus sequence characteristic of nif promoters, and functional analysis of the two promoters suggested that both nif operons are transcribed in noduIes.
The most widely used molecular marker system is based on the use of RFLPs. These are highly relia... more The most widely used molecular marker system is based on the use of RFLPs. These are highly reliable and informative but they are technically demanding to assay and expensive. Marker assays based on the Polymerase Chain Reaction (PCR) offer a far more user-friendly marker system. PCR based techniques such as RAPDs are unreliable and generally not transferable between crosses. However, microsatellites (di- or tri-nucleotide repeat sequences) also known as Simple Sequence Repeats (SSR) offer highly reliable, co-dominant markers. Microsatellites are becoming more widely used for marker assisted breeding and variety identification due to their high level of polymorphism and ease of use. Forty-three barley microsatellite markers were used to genotype 180 barley varieties. Primer pairs were synthesized and screened on a panel of four genetically diverse genotypes. Seventeen of them giving unambiguous and polymorphic DNA banding patterns were selected for allele typing study. Allele typing...
Harker, N, Hayden, M, Henry, RJ, Holton, TA, Karakousis, A, Langridge, P, McLauchlin, A, Morell, ... more Harker, N, Hayden, M, Henry, RJ, Holton, TA, Karakousis, A, Langridge, P, McLauchlin, A, Morell, MK, Preston, L, Shariflou, MR &amp; Sharp, PJ 1999, &#x27;Microsatellite analysis of Australian wheat varieties&#x27;, in JF Panozzo, M Ratcliffe, M Wooton &amp; CW Wrigley (eds), Cereals 99: Proceedings of the 49th Australian Cereal Chemistry Conference, Melbourne, Vic., 23-16 September, Cereal Chemistry Division, Royal Australian Chemical Institute, North Melbourne, Vic.
A full length cDNA clone encoding rose dihydroflavonol 4-reductase (DFR) was isolated from a cDNA... more A full length cDNA clone encoding rose dihydroflavonol 4-reductase (DFR) was isolated from a cDNA library derived from rose petals by screening with the cDNA of Petunia hybrida DFR. Sequence comparison of the rose DFR with reported DFR genes revealed that they are homologous to each other. The amount of DFR mRNA in rose petals was developmentally regulated and paralleled anthocyanin production in petals. Sepals, thorns, styles and stamens also contained anthocyanins and DFR mRNA. No DFR mRNA was observed in mature leaves and a small amount of the transcript was detected in young leaves. A petunia cultivar, whose colour was pale pink due to a deficiency in flavonoid 3'-hydroxylase and flavonoid 3',5'-hydroxylase, was transformed with a binary vector containing a rose DFR cDNA cloned behind a constitutive promoter. Petals and anthers of the resultant transgenic petunia plants were salmon pink and contained pelargonidin, an anthocyanidin rarely found in petunia.
... Flavonoid biosynthesis genes from grape (Vitis vinifera) isolated by EST analysis. Daniel LE ... more ... Flavonoid biosynthesis genes from grape (Vitis vinifera) isolated by EST analysis. Daniel LE Waters, Southern Cross University Timothy A. Holton Effie M. Ablett, Southern Cross University L Slade Lee, Southern Cross University Robert J. Henry, Southern Cross University. ...
The quality of tropical grasses is a major limitation to animal production in tropical and subtro... more The quality of tropical grasses is a major limitation to animal production in tropical and subtropical areas. This is mainly associated with the lower digestibility because C4 grasses have higher fibre levels. Any improvement in quality would require a reduction in the lignin and an increase in the digestion of the neutral detergent fibre content of these plants (Clark and Wilson 1993). Kikuyu (Pennisetum clandestinum) is an important grass for the dairy and beef industries of the subtropics of Australia, South Africa and New Zealand (Mears 1970). Increased digestibility could substantially improve animal production in these industries. These experiments investigated the variation in agronomic and quality of natural populations selected from diverse regions within Australia. Runners of 14 kikuyu selections were collected by project staff or local agronomists from areas considered to have grown kikuyu for over 30 years while Whittet and Noonan were established by seed. Entries were e...
Uploads
Papers by Timothy Holton