Papers by Thiago Rodrigues
Revista Árvore
The objective of this work was to evaluate the job conditions and aspects related to training, he... more The objective of this work was to evaluate the job conditions and aspects related to training, health and safety of forest fire crew members at Distrito Federal. The work was carried out in some Protected Areas including Água Limpa Farm, Ecological Reserve of Brazilian Institute of Geography and Statistics (IBGE) and Brasília Botanical Garden. The data was collected through individual interviews with 53 fire crew members. The results showed that the fire crew members of Brasília Botanical Garden had a high number of trained workers for forest fires (92,8%), all members obtained first aid courses and there was a high number of health problems (33,3%). The fire crew members of IBGE Ecological Reserve showed a high percentage of satisfaction with the activity (84,6%) and forest fire training (92,3%), all members obtained first aid courses and there was a low accident rate (7,7%). At Água Limpa Farm there were few fire crew members with training (39,1%), high percentage of accidents (17...
BioMed Research International, 2015
Repetitive element sequences are adjacent, repeating patterns, also called motifs, and can be of ... more Repetitive element sequences are adjacent, repeating patterns, also called motifs, and can be of different lengths; repetitions can involve their exact or approximate copies. They have been widely used as molecular markers in population biology. Given the sizes of sequenced genomes, various bioinformatics tools have been developed for the extraction of repetitive elements from DNA sequences. However, currently available tools do not provide options for identifying repetitive elements in the genome or proteome, displaying a user-friendly web interface, and performing-exhaustive searches. ProGeRF is a web site for extracting repetitive regions from genome and proteome sequences. It was designed to be efficient, fast, and accurate and primarily user-friendly web tool allowing many ways to view and analyse the results. ProGeRF (Proteome and Genome Repeat Finder) is freely available as a standalone program, from which the users can download the source code, and as a web tool. It was developed using the hash table approach to extract perfect and imperfect repetitive regions in a (multi)FASTA file, while allowing a linear time complexity.
International Journal of Atmospheric Sciences, 2013
The savanna of Central Brazil (locally known as cerrado) has a long history of land cover change ... more The savanna of Central Brazil (locally known as cerrado) has a long history of land cover change due to human activity. These changes have led to the degradation of cerrado forests and woodlands, leading to the expansion of grass-dominated cerrados and pastures. Thus, the aim of this study was to evaluate the temporal variation in energy flux in areas of degraded, grass-dominated cerrado (locally known as campo sujo) in Central Brazil. The amount of partitioned into H declined as monthly rainfall increased and reached a level of approximately 30% during the wet season, while the amount of partitioned into increased as monthly rainfall increased and reached a level of approximately 60% during the wet season. As a result, H was significantly higher than during the dry season, resulting in a Bowen ratio ( = H/ ) of 3-5, while Le was higher than H during the wet season, resulting in a ≈ 1. These data indicate that the energy partitioning of grass-dominated cerrado is relatively more sensitive to water availability than cerrado woodlands and forests, and have important implications for local and regional energy balance.
Journal of Geophysical Research: Biogeosciences, 2014
1] Tropical savanna (locally known as cerrado) composes 24% of Brazil and is characterized by hig... more 1] Tropical savanna (locally known as cerrado) composes 24% of Brazil and is characterized by high climatic variation; however, patterns of energy exchange are poorly understood, especially for mixed grasslands (locally known as campo sujo). We used eddy covariance to measure latent (L e ) and sensible (H) heat flux of a mixed grassland and linked meteorological and remote sensing data to determine the controls on these fluxes. We hypothesized that (1) seasonal variations in H and L e would be large due to variations in precipitation; (2) ecosystem phenology, estimated using the enhanced vegetation index (EVI), would be the best predictor of seasonal variation in L e ; and (3) cerrado, transitional, and humid evergreen forests would have similar rates of average annual L e despite large seasonal variation in cerrado L e . Our data suggest that campo sujo exhibits large seasonal fluctuations in energy balance that are driven by rainfall and that responses to rainfall pulses are rapid and dynamic, especially during the dry season. Path analysis indicated that temporal variations in the EVI did not significantly affect L e or G c , but this was because all three variables (EVI, L e , and G c ) responded similarly to temporal variations in surface water availability. Compared to other tropical ecosystems, wetter sites had higher rates of L e during the dry season but similar rates during the wet season when water was not limiting. Over annual time periods, average rates of L e increased significantly as average annual rainfall increased, due to dry-season water limitations in the more seasonal tropical ecosystems.
Revista brasileira de anestesiologia
Some formulations have been proposed to reduce the adverse reactions due to the lipid emulsion co... more Some formulations have been proposed to reduce the adverse reactions due to the lipid emulsion containing soybean oil used as propofol carrier. This study for endoscopy sedation was aimed at evaluating and comparing the safety, effectiveness and adverse effects of the use of propofol nanoemulsion compared to propofol currently commercialized. In this prospective study, 150 patients were submitted to upper digestive endoscopy. These patients were allocated into two groups: the control group (CONT Group; n=75) and the nanoemulsion group (NE Group; n=75). HR, SBP, DBP, SpO(2) and BIS (which is considered to be appropriate between 65 and 75 during procedure) were monitored. Gender, age, weight, height, BMI, ASA physical status, times and doses were analyzed, as well as adverse effects (phlogistic signs and pain on injection, apnea, nausea/vomiting) and alterations in monitoring variables. A p-value < 0.05 was considered significant. The groups had similar results concerning anthropom...
Archaea (Vancouver, B.C.), 2014
The Cerrado is a biome that corresponds to 24% of Brazil's territory. Only recently microbial... more The Cerrado is a biome that corresponds to 24% of Brazil's territory. Only recently microbial communities of this biome have been investigated. Here we describe for the first time the diversity of archaeal communities from freshwater lake sediments of the Cerrado in the dry season and in the transition period between the dry and rainy seasons, when the first rains occur. Gene libraries were constructed, using Archaea-specific primers for the 16S rRNA and amoA genes. Analysis revealed marked differences between the archaeal communities found in the two seasons. I.1a and I.1c Thaumarchaeota were found in greater numbers in the transition period, while MCG Archaea was dominant on the dry season. Methanogens were only found in the dry season. Analysis of 16S rRNA sequences revealed lower diversity on the transition period. We detected archaeal amoA sequences in both seasons, but there were more OTUs during the dry season. These sequences were within the same cluster as Nitrosotalea ...
Amino Acid Coding with Sliding Window Technique
Brazilian Workshop on Bioinformatics, 2003
Air, Soil and Water Research, 2013
Research involving the thermal soil dynamics of wetland areas has not yet been explored in a way ... more Research involving the thermal soil dynamics of wetland areas has not yet been explored in a way that promotes a deeper understanding of the dynamics of this region. This makes it necessary for further studies to contribute to the understanding of this biome. In the present work, we studied the thermal dynamics of the soil contrasting seasonal conditions in the Vochysia Divergens Forest. The Fourier equation was used to analyze the influence of the thermal conductivity and thermal gradient on the soil heat flux. We determined how variable water content causes the system to behave differently in the four seasons, observing seasonality in soil when completely dry and when completely flooded.
Semina: Ciências Agrárias, 2012
Pesquisas envolvendo a dinâmica térmica do solo pantaneiro ainda não foram exploradas de forma qu... more Pesquisas envolvendo a dinâmica térmica do solo pantaneiro ainda não foram exploradas de forma que se tenha um maior conhecimento dessa dinâmica na região, tornando-se necessário estudos que discutam suas propriedades térmicas, de forma a contribuir com o entendimento desse bioma. Dessa maneira, o presente trabalho analisou a variabilidade média do fluxo de calor no solo de um dia característico de cada período: chuvoso e seco, uma vez que a região apresenta uma sazonalidade no pulso de inundação e pluviosidade, afetando as trocas de energia entre a superfície e a atmosfera. Foi feita uma análise da influência da condutividade térmica e do gradiente térmico nos valores do fluxo de calor no solo. Observou-se que a variabilidade dos componentes térmicos do solo acompanhou o pulso de inundação da região pantaneira, pois a presença de água foi determinante na variação sazonal do fluxo de calor no solo, do gradiente de temperatura e da condutividade térmica do solo no Pantanal. Palavras-chave: Condutividade térmica, fluxo de calor no solo, gradiente de temperatura
Revista Brasileira de Meteorologia, 2013
Research involving the flux of energy in the soil has been intensified in order to increase the u... more Research involving the flux of energy in the soil has been intensified in order to increase the understanding of the geophysical behavior of the Pantanal-Brazil. In present study was examined the seasonal variation of the thermal soil conductivity in the Pantanal for the study of energy flow in the soil to Pantanal region. The average values obtained by the Fourier equation showed that the soil thermal conductivity in the wet and dry seasons was 8.69 W.m -1 .ºC -1 and 6.65 W.m -1 .ºC -1 respectively. The seasonal variation of the thermal conductivity of the soil was 30.68% higher in the wet season than in the dry season due to soil moisture in the wet season. It was also noted that the seasonal variation of temperature in the soil layer was higher in the wet season than in the dry season due to a lower incidence of solar radiation in this season.
Renewable Energy, 2014
Biomass has become important as an alternative to fossil fuels and as a means to decrease greenho... more Biomass has become important as an alternative to fossil fuels and as a means to decrease greenhouse gas (GHG) emissions, particularly in tropical regions such as Brazil. Therefore, the demand for energy crops has increased strongly, and among such crops, palm oil is distinctive because of its productivity and well-developed production techniques. This paper intends to evaluate crude palm oil's GHG balance through a life-cycle assessment approach. This study is based on the average data of an ideal palm oil system in the northern region of Brazil. In the production of crude palm oil, a large amount of CO 2 sequestration occurs during the growth of palm oil trees. In contrast, the greatest emissions are biotic CO 2 , which returns to the atmosphere and emissions from fertilizer production. The GHG balance of an oil palm plantation is approximately À208 kg CO 2 -equiv./1000 kg crude palm oil per year.
Molecular Genetics and Metabolism, 2011
Background and aims: Peroxisome proliferator-activated receptor α is a nuclear receptor involved ... more Background and aims: Peroxisome proliferator-activated receptor α is a nuclear receptor involved in the regulation of several biochemical pathways. Polymorphisms within its gene have been associated with several metabolic traits. We aimed to investigate the association of L162V and Intron 7GN C polymorphisms with serum level markers and common morbidities affecting an older adult/elderly cohort from Cuiaba City, Mato Grosso State, Brazil, as well as to compare the results with a previously studied population from São Paulo City, Brazil. Methods and results: The studied population consisted of 570 subjects from Cuiaba City, Brazil, who were subjected to clinical interviews and blood collection for laboratory examinations and DNA extraction. Dyslipidemia was defined when participants were taking oral hypolipemiants or those with total cholesterol above 200 mg/dL, HDL-c below 40 mg/dL, LDL-c above 130 mg/dL and TG above 150 mg/dL. Restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR) was used for polymorphism genotyping. Individual polymorphism and haplotype data were available for analyses. In the studied sample, allele frequencies were 0.052 and 0.292 for 162V and Intron 7C, respectively. In brief, 162V allele was associated with dyslipidemia (p = 0.025), and after correction for alcohol consumption and waist-to-rip ratio, a tendency of association could still be observed (p = 0.050). In addition, Intron 7C allele was associated with dyslipidemia even after correction for the same variables (p = 0.029). When compared to our previous study from São Paulo, we found some divergences regarding these results, which may be explained by differences between the two populations. Haplotype association analyses revealed an association between L/C haplotype and dyslipidemia (p =0.021) and between V/C haplotype and lower LDL-c levels when compared to L/G haplotype (p =0.044). Conclusion: These results may help to clarify the role of PPARα gene in lipid and lipoprotein metabolism and the evaluation of its polymorphisms and haplotypes as being characterized as genetic risk factors for metabolic disturbances.
Microbial Ecology, 2010
In order to characterize the bacterial community diversity associated to mucus of the coral Mussi... more In order to characterize the bacterial community diversity associated to mucus of the coral Mussismilia hispida, four 16S rDNA libraries were constructed and 400 clones from each library were analyzed from two healthy colonies, one diseased colony and the surrounding water. Nine bacterial phyla were identified in healthy M. hispida, with a dominance of Proteobacteria, Actinobacteria, Acidobacteria, Lentisphaerae, and Nitrospira. The most commonly found species were related to the genera Azospirillum, Hirschia, Fabibacter, Blastochloris, Stella, Vibrio, Flavobacterium, Ochrobactrum, Terasakiella, Alkalibacter, Staphylococcus, Azospirillum, Propionibacterium, Arcobacter, and Paenibacillus. In contrast, diseased M. hispida had a predominance of one single species of Bacteroidetes, corresponding to more than 70% of the sequences. Rarefaction curves using evolutionary distance of 1% showed a greater decrease in bacterial diversity in the diseased M. hispida, with a reduction of almost 85% in OTUs in comparison to healthy colonies. ∫-Libshuff analyses show that significant p values obtained were <0.0001, demonstrating that the four libraries are significantly different. Furthermore, the sympatric corals M. hispida and Mussismilia braziliensis appear to have different bacterial community compositions according to Principal Component Analysis and Lineage-specific Analysis. Moreover, lineages that contribute to those differences were identified as α-Proteobacteria, Bacteroidetes, and Firmicutes. The results obtained in this study suggest hostmicrobe co-evolution in Mussismilia, and it was the first study on the diversity of the microbiota of the endemic and endangered of extinction Brazilian coral M. hispida from Abrolhos bank.
Journal of Cleaner Production, 2011
This paper sets out to describe the environmental impact assessment for wood charcoal briquettes ... more This paper sets out to describe the environmental impact assessment for wood charcoal briquettes produced from eucalyptus wood in Brazil, with specific reference to those impacts associated with Global Warming Potential. To achieve that objective, the work was undertaken in accordance with ISO 14040 "Environmental management e Life cycle assessment e Principles and framework" which describes essential LCA characteristics and good practices. Charcoal briquettes are produced from two basic raw materials, charcoal fines and starch. The fines result from the production of charcoal from sustainably managed eucalyptus plantations. Starch is extracted from babaçu pulp in the Amazon region. Multi eoutput processes were allocated based on income from the different by-products. The results showed that more than 90% of incoming CO 2 was due to biomass production for charcoal, and the remainder to starch biomass production. Based on Brazilian data, as well as information provided by the GaBi4.3 database, it turned out that supplying the energy content of 1 kg of briquettes resulted in the sequestration of 3.9690 kg of CO 2 , i.e. around 4 kg of CO 2 per kg of briquettes produced. CO 2 emissions throughout the briquette production process are totally compensated for by the environmental quality of the raw materials used.
Protein Classification with Extended-Sequence Coding by Sliding Window
IEEE/ACM Transactions on Computational Biology and Bioinformatics, 2000
A large number of unclassified sequences is still found in public databases, which suggests that ... more A large number of unclassified sequences is still found in public databases, which suggests that there is still need for new investigations in the area. In this contribution, we present a methodology based on Artificial Neural Networks for protein functional classification. A new protein coding scheme, called here Extended-Sequence Coding by Sliding Windows, is presented with the goal of overcoming some of the difficulties of the well method Sequence Coding by Sliding Window. The new protein coding scheme uses more than one sliding window length with a weight factor that is proportional to the window length, avoiding the ambiguity problem without ignoring the identity of small subsequences Accuracy for Sequence Coding by Sliding Windows ranged from 60.1 to 77.7 percent for the first bacterium protein set and from 61.9 to 76.7 percent for the second one, whereas the accuracy for the proposed Extended-Sequence Coding by Sliding Windows scheme ranged from 70.7 to 97.1 percent for the first bacterium protein set and from 61.1 to 93.3 percent for the second one. Additionally, protein sequences classified inconsistently by the Artificial Neural Networks were analyzed by CD-Search revealing that there are some disagreement in public repositories, calling the attention for the relevant issue of error propagation in annotated databases due the incorrect transferred annotations.
Clustering and artificial neural networks: classification of variable lengths of Helminth antigens in set of domains
Genetics and Molecular Biology, 2004
A new scheme for representing proteins of different lengths in number of amino acids that can be ... more A new scheme for representing proteins of different lengths in number of amino acids that can be presented to a fixed number of inputs Artificial Neural Networks (ANNs) speel-out classification is described. K-Means&amp;#x27;s clustering of the new vectors with subsequent classification was ...
Association of APOE, GCPII and MMP9 polymorphisms with common diseases and lipid levels in an older adult/elderly cohort
Gene, 2014
The characterization of candidate gene polymorphisms in elderly populations is an important tool ... more The characterization of candidate gene polymorphisms in elderly populations is an important tool for the identification of risk factors for age-related diseases and conditions. We aimed to genotype the APOE polymorphisms (rs429358 and rs7412), rs61886492 (1561C&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;T) and rs202720 of GCPII gene and rs3918242 (-1562C&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;T) of MMP9 gene in an older-adult/elderly cohort from Cuiabá city, Mato Grosso Brazil as well as to characterize risk factors for morbidities and conditions affecting this cohort. The studied population consisted of 570 subjects from Cuiabá city, Brazil, who were subjected to clinical interviews and blood collection for laboratory examinations and DNA extraction. Restriction Fragment Length Polymorphism Polymerase Chain Reaction (RFLP-PCR), sequence-specific primer PCR (SSP-PCR) and TaqMan® allelic discrimination assay were used for genotyping. The frequencies of APOE ε2 and ε4 were 6.6% and 14.8%, respectively, and the frequencies of GCPII rs61886492 T allele, GCPII rs202720 C allele and MMP9 rs3918242 T allele were, respectively, 3.0%, 26.6% and 10.1%. Significant associations between APOE ε2 allele with lower total cholesterol and LDL-cholesterol were found. In addition, MMP9 rs3918242 T allele was associated with higher LDL-cholesterol levels, suggesting a link between lipid metabolism alteration and cardiovascular disease. The present findings contributed to characterize risk factors specific for the studied population and to better understand the molecular physiopathology of common morbidities and conditions affecting older-adult/elderly people.
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Papers by Thiago Rodrigues