Menopause is a stage in a woman’s life characterized by twelve months of amenorrhoea. This transi... more Menopause is a stage in a woman’s life characterized by twelve months of amenorrhoea. This transition happens due to changes in ovarian follicular activity, leading to endocrine, biological and clinical modifications. The main hormones related to these changes and symptoms are oestradiol, LH, FSH, AMH, Inhibin B and GnRH. It is important to point out that the skin is very affected by all these hormone changes, leading to a decrease in collagen content, water content, elasticity, thickness and impacting on all skin layers quality. Aiming to help women go through this period of their lifetimes with a better quality of life, cosmetic and pharmaceutical industries have studied formulations to improve skin quality. In order to study the safety and efficacy of these products, in vitro methods have been developed in order to mimic menopause and aged skin. In addition to that, many clinical methodologies for skin features assessment have also been improved and applied to evaluate the effica...
Mesenchymal stem cells (MSCs) are undifferentiated cells that are capable to self-renew and diffe... more Mesenchymal stem cells (MSCs) are undifferentiated cells that are capable to self-renew and differentiate into many cell types. These cells have been largely evaluated when it comes to therapies for cardiovascular diseases in order to recover the damaged tissue. It is known that the biological behavior of MSCs depends on the microenvironment where they are, which is mainly composed of cells, extracellular matrix (ECM) and soluble factors. Thus, we isolated resident cardiac cells from human auricle and ventricle by explant culture technique and then we characterized these cells’ phenotype, ECM and conditioned medium (soluble factors). We found that both cell populations are composed of MSCs, cardiac fibroblasts and possibly pericytes/myofibroblasts. These cells were cultured on monolayer and then we isolated their ECM by decellularization with a Triton X-100 0.5% solution. On the other hand, we obtained conditioned medium (CM) by both cell populations. We observed that the CMs are co...
The admission in higher education brings changes for the student regarding teaching, learning, re... more The admission in higher education brings changes for the student regarding teaching, learning, relationships and future perspectives. Because the role of the university is to take care of the health of its community's members, this research had as its objective the analyses of conducts and health habits, with a sample of 352 academic students in a public university of Parana State. The study involved the use of qualitative and quantitative methodology. It was applied a questionnaire aiming to diagnose lifestyles related to the health, such as feeding behavior, sleeping, hydration, physical activity, alcohol consumption, tobacco and illicit drugs use. The main events of health that have interfered in the student activities were reported. The most relevant points regarding the university freshmen’s conduct are the lack of physical exercises, the high levels of alcohol consumption and a significant percentage of sexual practices that
Anais do I Simpósio Internacional de Imunobiológicos: os atuais desafios para a construção de embiente favorável à inovação e ao desenvolvimento tecnológico
Toxicology in vitro : an international journal published in association with BIBRA, Jan 17, 2018
Nanoparticles (NPs) have emerged as new potential tools for many applications in previous years. ... more Nanoparticles (NPs) have emerged as new potential tools for many applications in previous years. Among all types of NPs, bismuth NPs (BiNPs) have a very low cost and potential for many applications, ranging from medicine to industry. Although the toxic effects of bismuth have been studied, little is known about its toxicity at the nanoscale level. Therefore, in this study, we aimed to investigate the cytotoxic effects of BiNPs produced by laser ablation synthesis in solution (LASiS) in a reference mammalian cell line to evaluate their cytotoxicity (BALB/c 3 T3 cells). We also stabilized BiNPs in two different solutions: culture medium supplemented with fetal bovine serum (FBS) and bovine serum albumin (BSA). The cytotoxicity of BiNPs in culture medium (IC:28.51 ± 9.96 μg/ml) and in BSA (IC:25.54 ± 8.37 μg/ml) was assessed, and they were not significantly different. Second, the LD was predicted, and BiNPs were estimated as GHS class 4. We also found that cell death occurs due to apop...
Apoptosis : an international journal on programmed cell death, 2017
Some therapeutics for parasitic, cardiac and neurological diseases activate apoptosis. Therefore,... more Some therapeutics for parasitic, cardiac and neurological diseases activate apoptosis. Therefore, the study of apoptotic proteins in pathogenic organisms is relevant. However, the molecular mechanism of apoptosis in unicellular organisms remain elusive, despite morphological evidence of its occurrence. In Trypanosoma cruzi, the causative agent of Chagas disease, metacaspase 3 (TcMCA3), seems to have a key role in parasite apoptosis. Accordingly, this work provides data concerning TcMCA3 regulation through its interaction with procaspase-activating compound 1 (PAC-1), a procaspase 3 activator. Indeed, PAC-1 reduced T. cruzi epimastigote viability with an IC50 of 14.12 µM and induced loss of mitochondrial potential and exposure of phosphatidylserine, features of the apoptotic process. Notwithstanding, those PAC-1-inducible effects were not conserved in metacyclic trypomastigotes. Moreover, PAC-1 reduced the viability of mammalian cells with a greater IC50 (25.70 µM) compared to T. cru...
In the heart, tissue-derived signals play a central role on recruiting/activating stem cell sourc... more In the heart, tissue-derived signals play a central role on recruiting/activating stem cell sources to induce cardiac lineage specification for maintenance of tissue homeostasis and repair. Cardiac resident stromal cells (CRSCs) may play a pivotal role in cardiac repair throughout their secretome. Here, we performed the characterization of CRSCs and their secretome by analyzing the composition of their culture-derived extracellular matrix (ECM) and conditioned medium (CM) and by investigating their potential effect on adipose-derived stem cell (ADSC) and progenitor cell behavior. We confirmed that CRSCs are a heterogeneous cell population whose secretome is composed by proteins related to cellular growth, immune response and cardiovascular development and function. We also observed that CRSC secretome was unable to change the behavior of ADSCs, except for proliferation. Additionally, CM from CRSCs demonstrated the potential to drive proliferation and cardiac differentiation of H9c2 cells and also the ability to induce angiogenesis in vitro. Our data suggest that the CRSCs can be a source of important modulating signals for cardiac progenitor cell recruitment/activation.
El ingreso a la educación superior trae cambios para los estudiantes bajo la óptica de la educaci... more El ingreso a la educación superior trae cambios para los estudiantes bajo la óptica de la educación, el aprendizaje, las relaciones interpersonales y las perspectivas del futuro. Entendiendo que el papel de la universidad es cuidar de la salud de los miembros de su comunidad, este estudio tuvo como objetivo averiguar las conductas y los hábitos de salud en una muestra de 352 estudiantes ingresantes de una universidad pública del estado de Paraná. El estudio implicó el uso de la metodología cualitativa y cuantitativa. Fue aplicado un cuestionario con el objetivo de diagnosticar los estilos de vida relacionados con la salud, como alimentación, sueño, hidratación, actividad física, consumo de alcohol, uso de tabaco y drogas ilícitas. Los principales eventos de salud que han interferido en las actividades estudiantiles fueron registrados. Los puntos destacados en la conducta de los ingresantes son la limitación para la práctica de ejercicio físico, el elevado consumo de bebidas alcohólicas y un porcentaje significativo de prácticas sexuales que ameritan atención. En si tratar al IMC el promedio general encontrado en el diurne y nocturnal estaba de 22,81 kg/m 2. El nivel de la instrucción no se parece tener papel protector en la adopción del estilo de vida saludable. Los resultados pueden servir de ayuda a las universidades en el desarrollo de programas de promoción de la salud.
Human adipose-derived stem cells (ADSC) were evaluated as cell culture model for cytotoxicity ass... more Human adipose-derived stem cells (ADSC) were evaluated as cell culture model for cytotoxicity assay and toxicity prediction by using the neutral red uptake assay (NRU). In this study, we compared ADSC and the murine cell line BALB/c 3T3 clone A31 to predict the toxicity of 12 reference substances as recommended by the Interagency Coordinating Committee on the Validation of Alternative Methods. We predicted the LD50 for RC-rat-only weight and RC-rat-only millimole regressions for both cell culture models. For RC rat-only weight regression, both cells had the same accordance (50%), while for RC rat-only millimole regression, the accordance was 50% for ADSC and 42% for 3T3s. Thus, ADSC have similar capability for GHS class prediction as the 3T3 cell line for the evaluated reference substances. Therefore, ADSCs showed the potential to be considered a novel model for use in evaluating cytotoxicity in drug development and industry as well as for regulatory purposes to reduce or replace the use of laboratory animals with acceptable sensitivity for toxicity prediction in humans. These cells can be used to complete the results from other models, mainly because of its human origin. Moreover, it is less expensive in comparison with other existing models.
The human papilloma virus (HPV-human papilloma virus) can infect up to 80% of people, especially ... more The human papilloma virus (HPV-human papilloma virus) can infect up to 80% of people, especially sexually active. The risk of infection and symptoms are different between the sexes. It is known that there are over 150 serotypes and these are grouped by their tropism. Although most infections follow a benign course, the persistent infection with certain serotypes may lead to the development of cancer. The serotypes 16 and 18 are involved in a severe form of injury, resulting in cancer, particularly cervical cancer. Serotypes 6 and 11 are described as causes of anogenital warts. The virus, approximately 8.000 bp, it installs itself in the cell and through the expression of oncoproteins E6 and E7 leading to inhibition of proteins such as p53 and pRB, important in apoptosis and cell cycle arrest. Current vaccines generate immunity against serotypes 6, 11, 16 and 18. The vaccine can be tetravalent (four serotypes 6, 11, 16 and 18) or bivalent (16, 18). Both have cross-protection against infection by serotypes not included in the vaccines, but have no therapeutic character. This study was proposed to review progress on the HPV infection, the immunological aspects and prophylactic vaccines available.
Menopause is a stage in a woman’s life characterized by twelve months of amenorrhoea. This transi... more Menopause is a stage in a woman’s life characterized by twelve months of amenorrhoea. This transition happens due to changes in ovarian follicular activity, leading to endocrine, biological and clinical modifications. The main hormones related to these changes and symptoms are oestradiol, LH, FSH, AMH, Inhibin B and GnRH. It is important to point out that the skin is very affected by all these hormone changes, leading to a decrease in collagen content, water content, elasticity, thickness and impacting on all skin layers quality. Aiming to help women go through this period of their lifetimes with a better quality of life, cosmetic and pharmaceutical industries have studied formulations to improve skin quality. In order to study the safety and efficacy of these products, in vitro methods have been developed in order to mimic menopause and aged skin. In addition to that, many clinical methodologies for skin features assessment have also been improved and applied to evaluate the effica...
Mesenchymal stem cells (MSCs) are undifferentiated cells that are capable to self-renew and diffe... more Mesenchymal stem cells (MSCs) are undifferentiated cells that are capable to self-renew and differentiate into many cell types. These cells have been largely evaluated when it comes to therapies for cardiovascular diseases in order to recover the damaged tissue. It is known that the biological behavior of MSCs depends on the microenvironment where they are, which is mainly composed of cells, extracellular matrix (ECM) and soluble factors. Thus, we isolated resident cardiac cells from human auricle and ventricle by explant culture technique and then we characterized these cells’ phenotype, ECM and conditioned medium (soluble factors). We found that both cell populations are composed of MSCs, cardiac fibroblasts and possibly pericytes/myofibroblasts. These cells were cultured on monolayer and then we isolated their ECM by decellularization with a Triton X-100 0.5% solution. On the other hand, we obtained conditioned medium (CM) by both cell populations. We observed that the CMs are co...
The admission in higher education brings changes for the student regarding teaching, learning, re... more The admission in higher education brings changes for the student regarding teaching, learning, relationships and future perspectives. Because the role of the university is to take care of the health of its community's members, this research had as its objective the analyses of conducts and health habits, with a sample of 352 academic students in a public university of Parana State. The study involved the use of qualitative and quantitative methodology. It was applied a questionnaire aiming to diagnose lifestyles related to the health, such as feeding behavior, sleeping, hydration, physical activity, alcohol consumption, tobacco and illicit drugs use. The main events of health that have interfered in the student activities were reported. The most relevant points regarding the university freshmen’s conduct are the lack of physical exercises, the high levels of alcohol consumption and a significant percentage of sexual practices that
Anais do I Simpósio Internacional de Imunobiológicos: os atuais desafios para a construção de embiente favorável à inovação e ao desenvolvimento tecnológico
Toxicology in vitro : an international journal published in association with BIBRA, Jan 17, 2018
Nanoparticles (NPs) have emerged as new potential tools for many applications in previous years. ... more Nanoparticles (NPs) have emerged as new potential tools for many applications in previous years. Among all types of NPs, bismuth NPs (BiNPs) have a very low cost and potential for many applications, ranging from medicine to industry. Although the toxic effects of bismuth have been studied, little is known about its toxicity at the nanoscale level. Therefore, in this study, we aimed to investigate the cytotoxic effects of BiNPs produced by laser ablation synthesis in solution (LASiS) in a reference mammalian cell line to evaluate their cytotoxicity (BALB/c 3 T3 cells). We also stabilized BiNPs in two different solutions: culture medium supplemented with fetal bovine serum (FBS) and bovine serum albumin (BSA). The cytotoxicity of BiNPs in culture medium (IC:28.51 ± 9.96 μg/ml) and in BSA (IC:25.54 ± 8.37 μg/ml) was assessed, and they were not significantly different. Second, the LD was predicted, and BiNPs were estimated as GHS class 4. We also found that cell death occurs due to apop...
Apoptosis : an international journal on programmed cell death, 2017
Some therapeutics for parasitic, cardiac and neurological diseases activate apoptosis. Therefore,... more Some therapeutics for parasitic, cardiac and neurological diseases activate apoptosis. Therefore, the study of apoptotic proteins in pathogenic organisms is relevant. However, the molecular mechanism of apoptosis in unicellular organisms remain elusive, despite morphological evidence of its occurrence. In Trypanosoma cruzi, the causative agent of Chagas disease, metacaspase 3 (TcMCA3), seems to have a key role in parasite apoptosis. Accordingly, this work provides data concerning TcMCA3 regulation through its interaction with procaspase-activating compound 1 (PAC-1), a procaspase 3 activator. Indeed, PAC-1 reduced T. cruzi epimastigote viability with an IC50 of 14.12 µM and induced loss of mitochondrial potential and exposure of phosphatidylserine, features of the apoptotic process. Notwithstanding, those PAC-1-inducible effects were not conserved in metacyclic trypomastigotes. Moreover, PAC-1 reduced the viability of mammalian cells with a greater IC50 (25.70 µM) compared to T. cru...
In the heart, tissue-derived signals play a central role on recruiting/activating stem cell sourc... more In the heart, tissue-derived signals play a central role on recruiting/activating stem cell sources to induce cardiac lineage specification for maintenance of tissue homeostasis and repair. Cardiac resident stromal cells (CRSCs) may play a pivotal role in cardiac repair throughout their secretome. Here, we performed the characterization of CRSCs and their secretome by analyzing the composition of their culture-derived extracellular matrix (ECM) and conditioned medium (CM) and by investigating their potential effect on adipose-derived stem cell (ADSC) and progenitor cell behavior. We confirmed that CRSCs are a heterogeneous cell population whose secretome is composed by proteins related to cellular growth, immune response and cardiovascular development and function. We also observed that CRSC secretome was unable to change the behavior of ADSCs, except for proliferation. Additionally, CM from CRSCs demonstrated the potential to drive proliferation and cardiac differentiation of H9c2 cells and also the ability to induce angiogenesis in vitro. Our data suggest that the CRSCs can be a source of important modulating signals for cardiac progenitor cell recruitment/activation.
El ingreso a la educación superior trae cambios para los estudiantes bajo la óptica de la educaci... more El ingreso a la educación superior trae cambios para los estudiantes bajo la óptica de la educación, el aprendizaje, las relaciones interpersonales y las perspectivas del futuro. Entendiendo que el papel de la universidad es cuidar de la salud de los miembros de su comunidad, este estudio tuvo como objetivo averiguar las conductas y los hábitos de salud en una muestra de 352 estudiantes ingresantes de una universidad pública del estado de Paraná. El estudio implicó el uso de la metodología cualitativa y cuantitativa. Fue aplicado un cuestionario con el objetivo de diagnosticar los estilos de vida relacionados con la salud, como alimentación, sueño, hidratación, actividad física, consumo de alcohol, uso de tabaco y drogas ilícitas. Los principales eventos de salud que han interferido en las actividades estudiantiles fueron registrados. Los puntos destacados en la conducta de los ingresantes son la limitación para la práctica de ejercicio físico, el elevado consumo de bebidas alcohólicas y un porcentaje significativo de prácticas sexuales que ameritan atención. En si tratar al IMC el promedio general encontrado en el diurne y nocturnal estaba de 22,81 kg/m 2. El nivel de la instrucción no se parece tener papel protector en la adopción del estilo de vida saludable. Los resultados pueden servir de ayuda a las universidades en el desarrollo de programas de promoción de la salud.
Human adipose-derived stem cells (ADSC) were evaluated as cell culture model for cytotoxicity ass... more Human adipose-derived stem cells (ADSC) were evaluated as cell culture model for cytotoxicity assay and toxicity prediction by using the neutral red uptake assay (NRU). In this study, we compared ADSC and the murine cell line BALB/c 3T3 clone A31 to predict the toxicity of 12 reference substances as recommended by the Interagency Coordinating Committee on the Validation of Alternative Methods. We predicted the LD50 for RC-rat-only weight and RC-rat-only millimole regressions for both cell culture models. For RC rat-only weight regression, both cells had the same accordance (50%), while for RC rat-only millimole regression, the accordance was 50% for ADSC and 42% for 3T3s. Thus, ADSC have similar capability for GHS class prediction as the 3T3 cell line for the evaluated reference substances. Therefore, ADSCs showed the potential to be considered a novel model for use in evaluating cytotoxicity in drug development and industry as well as for regulatory purposes to reduce or replace the use of laboratory animals with acceptable sensitivity for toxicity prediction in humans. These cells can be used to complete the results from other models, mainly because of its human origin. Moreover, it is less expensive in comparison with other existing models.
The human papilloma virus (HPV-human papilloma virus) can infect up to 80% of people, especially ... more The human papilloma virus (HPV-human papilloma virus) can infect up to 80% of people, especially sexually active. The risk of infection and symptoms are different between the sexes. It is known that there are over 150 serotypes and these are grouped by their tropism. Although most infections follow a benign course, the persistent infection with certain serotypes may lead to the development of cancer. The serotypes 16 and 18 are involved in a severe form of injury, resulting in cancer, particularly cervical cancer. Serotypes 6 and 11 are described as causes of anogenital warts. The virus, approximately 8.000 bp, it installs itself in the cell and through the expression of oncoproteins E6 and E7 leading to inhibition of proteins such as p53 and pRB, important in apoptosis and cell cycle arrest. Current vaccines generate immunity against serotypes 6, 11, 16 and 18. The vaccine can be tetravalent (four serotypes 6, 11, 16 and 18) or bivalent (16, 18). Both have cross-protection against infection by serotypes not included in the vaccines, but have no therapeutic character. This study was proposed to review progress on the HPV infection, the immunological aspects and prophylactic vaccines available.
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